Restriction map for pF1KB6272
 (Linear) (Six-base) MAPSORT of: KIBB6272.seq  Check: 7497  from: 1  to: 2319



KIBB6272.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AclI AA'CG_TT

Cuts at:      0    1089    2319 
   Size:       1089    1230
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     836    2319 
   Size:        836    1483
 
AhdI GACnn_n'nnGTC

Cuts at:      0     561    2319 
   Size:        561    1758
 
AleI CACnn'nnGTG

Cuts at:      0     794     974    2319 
   Size:        794     180    1345

  Fragments arranged by size: 

                1345     794     180
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1067    2319 
   Size:       1067    1252
 
ApaI G_GGCC'C

Cuts at:      0    1525    2319 
   Size:       1525     794
 
AvaI C'yCGr_G

Cuts at:      0     168    2030    2319 
   Size:        168    1862     289

  Fragments arranged by size: 

                1862     289     168
 
BanI G'GyrC_C

Cuts at:      0      33    2319 
   Size:         33    2286
 
BanII G_rGCy'C

Cuts at:      0     628     991    1525    1609    2078    2319 
   Size:        628     363     534      84     469     241

  Fragments arranged by size: 

                 628     534     469     363     241      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     464    1126    2319 
   Size:        464     662    1193

  Fragments arranged by size: 

                1193     662     464
 
BbvCI CC'TCA_GC

Cuts at:      0    1113    2319 
   Size:       1113    1206
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1274    1308    2319 
   Size:       1274      34    1011

  Fragments arranged by size: 

                1274    1011      34
 
BfrBI ATG'CAT

Cuts at:      0     309    1560    2319 
   Size:        309    1251     759

  Fragments arranged by size: 

                1251     759     309
 
BglII A'GATC_T

Cuts at:      0    1948    2319 
   Size:       1948     371
 
Bme1580I G_kGCm'C

Cuts at:      0    1525    2319 
   Size:       1525     794
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     738    2319 
   Size:        738    1581
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     162     821     978    1286    1289    2188    2319 
   Size:         37     125     659     157     308       3     899     131

  Fragments arranged by size: 

                 899     659     308     157     131     125      37       3
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     471    1113    1608    2319 
   Size:        471     642     495     711

  Fragments arranged by size: 

                 711     642     495     471
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2086    2319 
   Size:       2086     233
 
BsaBI GATnn'nnATC

Cuts at:      0     965    2319 
   Size:        965    1354
 
BsaWI w'CCGG_w

Cuts at:      0    1505    1722    2319 
   Size:       1505     217     597

  Fragments arranged by size: 

                1505     597     217
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     184     214    2319 
   Size:        184      30    2105

  Fragments arranged by size: 

                2105     184      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     981    1101    1349    1815    2319 
   Size:         31     950     120     248     466     504

  Fragments arranged by size: 

                 950     504     466     248     120      31
 
BseYI C'CCAG_C

Cuts at:      0     265    2319 
   Size:        265    2054
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113    2110    2319 
   Size:        113    1997     209

  Fragments arranged by size: 

                1997     209     113
 
BsiEI CG_ry'CG

Cuts at:      0    2082    2319 
   Size:       2082     237
 
BsiHKAI G_wGCw'C

Cuts at:      0     991    2078    2319 
   Size:        991    1087     241

  Fragments arranged by size: 

                1087     991     241
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1424    2285    2319 
   Size:       1424     861      34

  Fragments arranged by size: 

                1424     861      34
 
Bsp1286I G_dGCh'C

Cuts at:      0     628     991    1525    1609    2078    2319 
   Size:        628     363     534      84     469     241

  Fragments arranged by size: 

                 628     534     469     363     241      84
 
BspEI T'CCGG_A

Cuts at:      0    1505    2319 
   Size:       1505     814
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1880    2319 
   Size:       1880     439
 
BsrBI CCG'CTC

Cuts at:      0    2010    2319 
   Size:       2010     309
 
BsrFI r'CCGG_y

Cuts at:      0    1249    1621    2269    2319 
   Size:       1249     372     648      50

  Fragments arranged by size: 

                1249     648     372      50
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     877    2319 
   Size:        877    1442
 
BstBI TT'CG_AA

Cuts at:      0     825    2319 
   Size:        825    1494
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     273    1162    2319 
   Size:        273     889    1157

  Fragments arranged by size: 

                1157     889     273
 
BstYI r'GATC_y

Cuts at:      0    1948    2319 
   Size:       1948     371
 
Bsu36I CC'TnA_GG

Cuts at:      0     333    2319 
   Size:        333    1986
 
BtgI C'CryG_G

Cuts at:      0     800    1592    2319 
   Size:        800     792     727

  Fragments arranged by size: 

                 800     792     727
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     391    2319 
   Size:        391    1928
 
BtsI GCAGTG_nn'

Cuts at:      0     405     875     981    1523    2319 
   Size:        405     470     106     542     796

  Fragments arranged by size: 

                 796     542     470     405     106
 
DraI TTT'AAA

Cuts at:      0     120    2319 
   Size:        120    2199
 
EaeI y'GGCC_r

Cuts at:      0     963    2018    2079    2150    2267    2319 
   Size:        963    1055      61      71     117      52

  Fragments arranged by size: 

                1055     963     117      71      61      52
 
EagI C'GGCC_G

Cuts at:      0    2079    2319 
   Size:       2079     240
 
EarI CTCTTCn'nnn_

Cuts at:      0     791    1044    1191    1709    2225    2319 
   Size:        791     253     147     518     516      94

  Fragments arranged by size: 

                 791     518     516     253     147      94
 
EcoICRI GAG'CTC

Cuts at:      0     989    2076    2319 
   Size:        989    1087     243

  Fragments arranged by size: 

                1087     989     243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     162     821     836     978    1286    1289    2188 
   Size:         37     125     659      15     142     308       3     899

Cuts at:   2188    2319 
   Size:        131

  Fragments arranged by size: 

                 899     659     308     142     131     125      37      15
                   3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1787    2140    2319 
   Size:       1787     353     179

  Fragments arranged by size: 

                1787     353     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0     185     476     555    1435    1661    1779    1971    2319 
   Size:        185     291      79     880     226     118     192     348

  Fragments arranged by size: 

                 880     348     291     226     192     185     118      79
            
 
EcoRV GAT'ATC

Cuts at:      0    1926    2319 
   Size:       1926     393
 
FseI GG_CCGG'CC

Cuts at:      0    2273    2319 
   Size:       2273      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     547     562     579     594    1287    1319    1634    1666 
   Size:        547      15      17      15     693      32     315      32

Cuts at:   1666    1831    1863    1870    1902    1915    1947    2224    2256 
   Size:        165      32       7      32      13      32     277      32

Cuts at:   2256    2319 
   Size:         63

  Fragments arranged by size: 

                 693     547     315     277     165      63      32      32
                  32      32      32      32      17      15      15      13
                   7
 
MscI TGG'CCA

Cuts at:      0     965    2020    2319 
   Size:        965    1055     299

  Fragments arranged by size: 

                1055     965     299
 
MslI CAynn'nnrTG

Cuts at:      0     700     794     974    1082    1160    1534    2319 
   Size:        700      94     180     108      78     374     785

  Fragments arranged by size: 

                 785     700     374     180     108      94      78
 
MspA1I CmG'CkG

Cuts at:      0    1384    2319 
   Size:       1384     935
 
NaeI GCC'GGC

Cuts at:      0    1251    2271    2319 
   Size:       1251    1020      48

  Fragments arranged by size: 

                1251    1020      48
 
NcoI C'CATG_G

Cuts at:      0     800    1592    2319 
   Size:        800     792     727

  Fragments arranged by size: 

                 800     792     727
 
NgoMIV G'CCGG_C

Cuts at:      0    1249    2269    2319 
   Size:       1249    1020      50

  Fragments arranged by size: 

                1249    1020      50
 
NsiI A_TGCA'T

Cuts at:      0     311    1562    2319 
   Size:        311    1251     757

  Fragments arranged by size: 

                1251     757     311
 
NspI r_CATG'y

Cuts at:      0     146    1560    1797    2319 
   Size:        146    1414     237     522

  Fragments arranged by size: 

                1414     522     237     146
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1518    2148    2281    2319 
   Size:       1518     630     133      38

  Fragments arranged by size: 

                1518     630     133      38
 
PfoI T'CCnGG_A

Cuts at:      0     178     508    2319 
   Size:        178     330    1811

  Fragments arranged by size: 

                1811     330     178
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183     214     246    2319 
   Size:        151      32      31      32    2073

  Fragments arranged by size: 

                2073     151      32      32      31
 
PpuMI rG'GwC_Cy

Cuts at:      0     476     555    1435    1661    1779    1971    2319 
   Size:        476      79     880     226     118     192     348

  Fragments arranged by size: 

                 880     476     348     226     192     118      79
 
PspOMI G'GGCC_C

Cuts at:      0    1521    2319 
   Size:       1521     798
 
PspXI vC'TCGA_Gb

Cuts at:      0     168    2319 
   Size:        168    2151
 
PvuII CAG'CTG

Cuts at:      0    1384    2319 
   Size:       1384     935
 
SacI G_AGCT'C

Cuts at:      0     991    2078    2319 
   Size:        991    1087     241

  Fragments arranged by size: 

                1087     991     241
 
SanDI GG'GwC_CC

Cuts at:      0    1661    2319 
   Size:       1661     658
 
ScaI AGT'ACT

Cuts at:      0     748    2319 
   Size:        748    1571
 
SexAI A'CCwGG_T

Cuts at:      0     735    1715    2319 
   Size:        735     980     604

  Fragments arranged by size: 

                 980     735     604
 
SfcI C'TryA_G

Cuts at:      0    1068    1807    2319 
   Size:       1068     739     512

  Fragments arranged by size: 

                1068     739     512
 
SmlI C'TyrA_G

Cuts at:      0     168    2101    2319 
   Size:        168    1933     218

  Fragments arranged by size: 

                1933     218     168
 
SphI G_CATG'C

Cuts at:      0    1797    2319 
   Size:       1797     522
 
StuI AGG'CCT

Cuts at:      0     783    1411    2319 
   Size:        783     628     908

  Fragments arranged by size: 

                 908     783     628
 
StyI C'CwwG_G

Cuts at:      0     188     282     558     684     800     982    1592    2264 
   Size:        188      94     276     126     116     182     610     672

Cuts at:   2264    2319 
   Size:         55

  Fragments arranged by size: 

                 672     610     276     188     182     126     116      94
                  55
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1585    2319 
   Size:       1585     734
 
TatI w'GTAC_w

Cuts at:      0     746    2319 
   Size:        746    1573
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     246     278    1653    1685    2319 
   Size:        246      32    1375      32     634

  Fragments arranged by size: 

                1375     634     246      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1393    2319 
   Size:       1393     926
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     186    1599    1672    2319 
   Size:        186    1413      73     647

  Fragments arranged by size: 

                1413     647     186      73
 
XhoI C'TCGA_G

Cuts at:      0     168    2319 
   Size:        168    2151
 
XmnI GAAnn'nnTTC

Cuts at:      0     720    2319 
   Size:        720    1599
 

 Enzymes that do cut:

     AclI     AcuI     AhdI     AleI    AlwNI     ApaI     AvaI     BanI
    BanII     BbsI    BbvCI     BcgI    BfrBI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FseI    Hin4I     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NsiI     NspI    PflMI     PfoI     PpiI    PpuMI
   PspOMI    PspXI    PvuII     SacI    SanDI     ScaI    SexAI     SfcI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BciVI     BclI     BglI     BlpI    BmgBI
     BmtI     BplI     BsaI    BsaAI    BsaHI    BsiWI     BsmI    BspHI
    BsrDI    BsrGI   BssHII    BssSI   BstEII  BstZ17I     ClaI    CspCI
   DraIII     DrdI     EciI    EcoRI     FalI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NarI     NdeI     NheI     NotI     NruI     PacI     PasI     PciI
     PmeI     PmlI    PshAI     PsiI     PsrI     PstI     PvuI    RsrII
    SacII     SalI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XmaI
     ZraI
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