Restriction map for pF1KB6194
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        ApaI        AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BseAI       BsiWI       BspDI       BspEI       
BspMI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DrdI        
EcoNI       EcoO65I     FseI        FspI        Hin1I       
HindIII     KasI        KpnI        KspI        MfeI        
MluI        MroI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AflII         909  CTTAAG               C^TTAAG
AlwI          631  GGATC                GGATC(4/5)
AlwNI        1079  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1021  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       870  AGCGCT               AGC^GCT
AvaI         2095  CCCGGG               C^[CT]CG[GA]G
BanI          439  GGTGCC               G^G[CT][GA]CC
BclI         2045  TGATCA               T^GATCA
BfaI         1519  CTAG                 C^TAG
BfrI          909  CTTAAG               C^TTAAG
BpmI         1804  CTGGAG               CTGGAG(16/14)
BsaBI         129  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          955  GGTCTC               GGTCTC(1/5)
BsaWI         942  TCCGGT               [AT]^CCGG[AT]
BsgI          285  GTGCAG               GTGCAG(16/14)
BsiEI        1954  CGGCCG               CG[GA][CT]^CG
BsmBI         403  GAGACG               CGTCTC(1/5)
BsoBI        2095  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1336  TGTACA               T^GTACA
BspHI        2087  TCATGA               T^CATGA
BsrBI        2104  GAGCGG               CCGCTC(-3/-3)
BsrDI         578  GCAATG               GCAATG(2/0)
BsrGI        1336  TGTACA               T^GTACA
BstXI        1769  CCATCCGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1021  CCTCAGG              CC^T[ACGT]AGG
DraI          700  TTTAAA               TTT^AAA
DraIII       1672  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1954  CGGCCG               C^GGCCG
EclXI        1954  CGGCCG               C^GGCCG
Eco47III      870  AGCGCT               AGC^GCT
Eco52I       1954  CGGCCG               C^GGCCG
Eco81I       1021  CCTCAGG              CC^T[ACGT]AGG
EcoRV        2112  GATATC               GAT^ATC
EcoT22I       788  ATGCAT               ATGCA^T
FauI           41  GCGGG                CCCGC(4/6)
FbaI         2045  TGATCA               T^GATCA
HgaI         1323  GACGC                GACGC(5/10)
HincII         73  GTTAAC               GT[CT]^[GA]AC
HindII         73  GTTAAC               GT[CT]^[GA]AC
HpaI           73  GTTAAC               GTT^AAC
MaeI         1519  CTAG                 C^TAG
MaeIII        377  GTGAC                ^GT[ACGT]AC
MamI          129  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI          788  ATGCAT               ATGCA^T
PflMI        1671  CCACTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         2087  TCATGA               T^CATGA
SmaI         2095  CCCGGG               CCC^GGG
SspBI        1336  TGTACA               T^GTACA
StuI         1226  AGGCCT               AGG^CCT
Tsp45I        377  GTGAC                ^GT[GC]AC
Van91I       1671  CCACTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        2095  CCCGGG               C^CCGGG
XmaI         2095  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccI          706  GTCTAC               GT^[AC][GT]AC
             1501  GTCTAC              
AflIII       1328  ACATGT               A^C[GA][CT]GT
             1586  ACATGT              
AhdI           37  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1681  GACTGGTGGTC         
Alw44I        624  GTGCAC               G^TGCAC
             1269  GTGCAC              
ApaLI         624  GTGCAC               G^TGCAC
             1269  GTGCAC              
AspEI          37  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1681  GACTGGTGGTC         
BalI          830  TGGCCA               TGG^CCA
              875  TGGCCA              
BbsI          303  GAAGAC               GAAGAC(2/6)
             1180  GTCTTC              
BcgI          480  CGAGTTCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1982  GCAGCAATTTCG        
BglII         926  AGATCT               A^GATCT
             1646  AGATCT              
BpuAI         303  GAAGAC               GAAGAC(2/6)
             1180  GTCTTC              
BseRI        1750  GAGGAG               GAGGAG(10/8)
             1753  GAGGAG              
BsmI          341  GAATGC               GAATGC(1/-1)
              351  GAATGC              
BspLU11I     1328  ACATGT               A^CATGT
             1586  ACATGT              
BssSI         478  CACGAG               CACGAG(-5/-1)
              529  CACGAG              
BstYI         926  AGATCT               [GA]^GATC[CT]
             1646  AGATCT              
CfoI          432  GCGC                 GCG^C
              871  GCGC                
Eam1105I       37  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1681  GACTGGTGGTC         
EcoRI        1192  GAATTC               G^AATTC
             1999  GAATTC              
EcoT14I      1298  CCTTGG               C^C[AT][AT]GG
             1349  CCAAGG              
HaeII         431  AGCGCC               [GA]GCGC^[CT]
              870  AGCGCT              
HhaI          432  GCGC                 GCG^C
              871  GCGC                
HinP1I        432  GCGC                 G^CGC
              871  GCGC                
HphI         1830  GGTGA                GGTGA(8/7)
             1969  TCACC               
MflI          926  AGATCT               [GA]^GATC[CT]
             1646  AGATCT              
MluNI         830  TGGCCA               TGG^CCA
              875  TGGCCA              
MscI          830  TGGCCA               TGG^CCA
              875  TGGCCA              
MslI          798  CATGAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1770  CATCCGCATG          
SacI          262  GAGCTC               GAGCT^C
             1756  GAGCTC              
SapI         1758  GCTCTTC              GCTCTTC(1/4)
             1836  GCTCTTC             
StyI         1298  CCTTGG               C^C[AT][AT]GG
             1349  CCAAGG              
TfiI          112  GAATC                G^A[AT]TC
              674  GATTC               
XhoII         926  AGATCT               [GA]^GATC[CT]
             1646  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
Asp700I     AspHI       AvaII       BanII       BbvI        
BcnI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MaeII       MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XmnI        
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