Restriction map for
pF1KB6194
(Circular) (Six-base) MAPSORT of: pF1KB6194.seq Check: 131 from: 1 to: 5231
pF1KB6194 5231 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 815 1610 2269 815
Size: 795 659 3777
Fragments arranged by size:
3777 795 659
Acc65I G'GTAC_C
Cuts at: 2247 2247
Size: 5231
AclI AA'CG_TT
Cuts at: 5071 5071
Size: 5231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 1023 1642 3056 3488 4235 4503 995
Size: 28 619 1414 432 747 268 1723
Fragments arranged by size:
1723 1414 747 619 432 268 28
AfeI AGC'GCT
Cuts at: 980 980
Size: 5231
AflII C'TTAA_G
Cuts at: 1017 1017
Size: 5231
AflIII A'CryG_T
Cuts at: 1436 1694 2583 3687 4664 1436
Size: 258 889 1104 977 2003
Fragments arranged by size:
2003 1104 977 889 258
AgeI A'CCGG_T
Cuts at: 3619 3619
Size: 5231
AhdI GACnn_n'nnGTC
Cuts at: 150 1794 150
Size: 1644 3587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 928 928
Size: 5231
AlwNI CAG_nnn'CTG
Cuts at: 1192 2536 4103 1192
Size: 1344 1567 2320
Fragments arranged by size:
2320 1567 1344
ApaLI G'TGCA_C
Cuts at: 732 1377 4001 732
Size: 645 2624 1962
Fragments arranged by size:
2624 1962 645
ApoI r'AATT_y
Cuts at: 505 1300 2107 2235 4584 4840 505
Size: 795 807 128 2349 256 896
Fragments arranged by size:
2349 896 807 795 256 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 5231
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5231
AvaI C'yCGr_G
Cuts at: 2203 2251 2203
Size: 48 5183
BamHI G'GATC_C
Cuts at: 2256 2256
Size: 5231
BanI G'GyrC_C
Cuts at: 547 2247 2661 2909 2944 547
Size: 1700 414 248 35 2834
Fragments arranged by size:
2834 1700 414 248 35
BanII G_rGCy'C
Cuts at: 374 832 1868 2245 3275 374
Size: 458 1036 377 1030 2330
Fragments arranged by size:
2330 1036 1030 458 377
BbeI G_GCGC'C
Cuts at: 2665 2913 2665
Size: 248 4983
BbsI GAAGACnn'nnnn_
Cuts at: 418 1281 418
Size: 863 4368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 577 611 2079 2113 577
Size: 34 1468 34 3695
Fragments arranged by size:
3695 1468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3129 3890 5231 3129
Size: 761 1341 3129
Fragments arranged by size:
3129 1341 761
BclI T'GATC_A
Cuts at: 2153 2751 2153
Size: 598 4633
BfrBI ATG'CAT
Cuts at: 898 898
Size: 5231
BglII A'GATC_T
Cuts at: 1034 1754 2746 4783 1034
Size: 720 992 2037 1482
Fragments arranged by size:
2037 1482 992 720
BlpI GC'TnA_GC
Cuts at: 2343 2343
Size: 5231
Bme1580I G_kGCm'C
Cuts at: 736 1381 2856 2949 4005 736
Size: 645 1475 93 1056 1962
Fragments arranged by size:
1962 1475 1056 645 93
BmgBI CAC'GTC
Cuts at: 576 576
Size: 5231
BmrI ACTGGGnnnn_n'
Cuts at: 2031 2443 2524 2859 5003 2031
Size: 412 81 335 2144 2259
Fragments arranged by size:
2259 2144 412 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 393 3598 393
Size: 3205 2026
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1933 1933
Size: 5231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 871 2407 3286 3778 4076 425
Size: 446 1536 879 492 298 1580
Fragments arranged by size:
1580 1536 879 492 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 1069 4935 1069
Size: 3866 1365
BsaAI yAC'GTr
Cuts at: 2584 2584
Size: 5231
BsaBI GATnn'nnATC
Cuts at: 6 241 2745 4782 6
Size: 235 2504 2037 455
Fragments arranged by size:
2504 2037 455 235
BsaHI Gr'CG_yC
Cuts at: 2662 2910 3612 5108 2662
Size: 248 702 1496 2785
Fragments arranged by size:
2785 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1050 2420 2941 3619 3893 4040 4451 1050
Size: 1370 521 678 274 147 411 1830
Fragments arranged by size:
1830 1370 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1138 1168 1408 1438 1681 1711 1138
Size: 30 240 30 243 30 4658
Fragments arranged by size:
4658 243 240 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1873 1876 1873
Size: 3 5228
BseYI C'CCAG_C
Cuts at: 1761 1971 2656 3414 3991 4487 1761
Size: 210 685 758 577 496 2505
Fragments arranged by size:
2505 758 685 577 496 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 414
Size: 5231
BsiEI CG_ry'CG
Cuts at: 104 2065 2773 2819 4027 104
Size: 1961 708 46 1208 1308
Fragments arranged by size:
1961 1308 1208 708 46
BsiHKAI G_wGCw'C
Cuts at: 203 374 736 1000 1381 1868 2151 2245 3023
Size: 171 362 264 381 487 283 94 778
Cuts at: 3023 3213 4005 203
Size: 190 792 1429
Fragments arranged by size:
1429 792 778 487 381 362 283 264
190 171 94
BsmI GAATG_Cn'
Cuts at: 455 465 455
Size: 10 5221
BsmBI CGTCTCn'nnnn_
Cuts at: 505 505
Size: 5231
Bsp1286I G_dGCh'C
Cuts at: 203 374 736 832 1000 1381 1868 2151 2245
Size: 171 362 96 168 381 487 283 94
Cuts at: 2245 2856 2949 3023 3213 3275 4005 203
Size: 611 93 74 190 62 730 1429
Fragments arranged by size:
1429 730 611 487 381 362 283 190
171 168 96 94 93 74 62
BspEI T'CCGG_A
Cuts at: 4451 4451
Size: 5231
BspHI T'CATG_A
Cuts at: 2195 4407 2195
Size: 2212 3019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2281 2495 2797 3178 2281
Size: 214 302 381 4334
Fragments arranged by size:
4334 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2214 3523 3577 5061 5226 50
Size: 2164 1309 54 1484 165 55
Fragments arranged by size:
2164 1484 1309 165 55 54
BsrDI GCAATG_nn'
Cuts at: 693 3143 693
Size: 2450 2781
BsrFI r'CCGG_y
Cuts at: 3229 3410 3619 3229
Size: 181 209 4841
Fragments arranged by size:
4841 209 181
BsrGI T'GTAC_A
Cuts at: 1444 1444
Size: 5231
BssSI C'ACGA_G
Cuts at: 586 637 3502 3860 586
Size: 51 2865 358 1957
Fragments arranged by size:
2865 1957 358 51
BstAPI GCAn_nnn'nTGC
Cuts at: 2608 2608
Size: 5231
BstBI TT'CG_AA
Cuts at: 3592 3592
Size: 5231
BstXI CCAn_nnnn'nTGG
Cuts at: 1884 1884
Size: 5231
BstYI r'GATC_y
Cuts at: 1 1034 1754 2256 2723 2746 3081 3327 4339
Size: 1033 720 502 467 23 335 246 1012
Cuts at: 4339 4425 4437 4783 1
Size: 86 12 346 449
Fragments arranged by size:
1033 1012 720 502 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1130 1130
Size: 5231
BtgI C'CryG_G
Cuts at: 365 1845 1903 365
Size: 1480 58 3693
Fragments arranged by size:
3693 1480 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1728 3181 3362 3525 1728
Size: 1453 181 163 3434
Fragments arranged by size:
3434 1453 181 163
BtsI GCAGTG_nn'
Cuts at: 593 2474 593
Size: 1881 3350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 930 965 930
Size: 35 5196
DraI TTT'AAA
Cuts at: 810 2230 810
Size: 1420 3811
DraIII CAC_nnn'GTG
Cuts at: 1785 1785
Size: 5231
DrdI GACnn_nn'nnGTC
Cuts at: 2937 3795 2937
Size: 858 4373
EaeI y'GGCC_r
Cuts at: 938 983 2062 2816 2990 3381 3408 4753 938
Size: 45 1079 754 174 391 27 1345 1416
Fragments arranged by size:
1416 1345 1079 754 391 174 45 27
EagI C'GGCC_G
Cuts at: 2062 2816 2062
Size: 754 4477
EarI CTCTTCn'nnn_
Cuts at: 303 1387 1873 1951 1960 3254 3464 303
Size: 1084 486 78 9 1294 210 2070
Fragments arranged by size:
2070 1294 1084 486 210 78 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1981 3439 3749 3895 5041 1981
Size: 1458 310 146 1146 2171
Fragments arranged by size:
2171 1458 1146 310 146
EcoICRI GAG'CTC
Cuts at: 372 1866 2243 372
Size: 1494 377 3360
Fragments arranged by size:
3360 1494 377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 1023 1642 1933 3056 3488 4235 4503 995
Size: 28 619 291 1123 432 747 268 1723
Fragments arranged by size:
1723 1123 747 619 432 291 268 28
EcoO109I rG'GnC_Cy
Cuts at: 152 651 854 1617 1929 2370 152
Size: 499 203 763 312 441 3013
Fragments arranged by size:
3013 763 499 441 312 203
EcoRI G'AATT_C
Cuts at: 1300 2107 2235 1300
Size: 807 128 4296
Fragments arranged by size:
4296 807 128
EcoRV GAT'ATC
Cuts at: 2222 2222
Size: 5231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 5231
FspI TGC'GCA
Cuts at: 3012 3012
Size: 5231
HaeII r_GCGC'y
Cuts at: 543 982 2665 2913 3935 4914 543
Size: 439 1683 248 1022 979 860
Fragments arranged by size:
1683 1022 979 860 439 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 337 369 731 763 797 829 1780
Size: 32 169 32 362 32 34 32 951
Cuts at: 1780 1812 2138 2170 3073 3103 3105 3135 136
Size: 32 326 32 903 30 2 30 2232
Fragments arranged by size:
2232 951 903 362 326 169 34 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 183 2270 183
Size: 2087 3144
HpaI GTT'AAC
Cuts at: 183 183
Size: 5231
KasI G'GCGC_C
Cuts at: 2661 2909 2661
Size: 248 4983
KpnI G_GTAC'C
Cuts at: 2251 2251
Size: 5231
MluI A'CGCG_T
Cuts at: 4664 4664
Size: 5231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 1136 3902 4086 161
Size: 975 2766 184 1306
Fragments arranged by size:
2766 1306 975 184
MscI TGG'CCA
Cuts at: 940 985 2992 940
Size: 45 2007 3179
Fragments arranged by size:
3179 2007 45
MslI CAynn'nnrTG
Cuts at: 910 1882 3220 3347 910
Size: 972 1338 127 2794
Fragments arranged by size:
2794 1338 972 127
MspA1I CmG'CkG
Cuts at: 2342 2656 3016 4029 4274 2342
Size: 314 360 1013 245 3299
Fragments arranged by size:
3299 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3412 3412
Size: 5231
NarI GG'CG_CC
Cuts at: 2662 2910 2662
Size: 248 4983
NgoMIV G'CCGG_C
Cuts at: 3410 3410
Size: 5231
NsiI A_TGCA'T
Cuts at: 900 900
Size: 5231
NspI r_CATG'y
Cuts at: 798 902 1440 1698 2284 2784 3691 798
Size: 104 538 258 586 500 907 2338
Fragments arranged by size:
2338 907 586 538 500 258 104
PasI CC'CwG_GG
Cuts at: 272 272
Size: 5231
PciI A'CATG_T
Cuts at: 1436 1694 3687 1436
Size: 258 1993 2980
Fragments arranged by size:
2980 1993 258
PflMI CCAn_nnn'nTGG
Cuts at: 1785 1785
Size: 5231
PmeI GTTT'AAAC
Cuts at: 2230 2230
Size: 5231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 4370
Size: 32 5199
PpuMI rG'GwC_Cy
Cuts at: 152 854 1617 1929 152
Size: 702 763 312 3454
Fragments arranged by size:
3454 763 702 312
PstI C_TGCA'G
Cuts at: 1223 1586 2092 2278 2963 1223
Size: 363 506 186 685 3491
Fragments arranged by size:
3491 685 506 363 186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5231
PvuII CAG'CTG
Cuts at: 2656 3016 2656
Size: 360 4871
RsrII CG'GwC_CG
Cuts at: 3426 3426
Size: 5231
SacI G_AGCT'C
Cuts at: 374 1868 2245 374
Size: 1494 377 3360
Fragments arranged by size:
3360 1494 377
SalI G'TCGA_C
Cuts at: 2268 2268
Size: 5231
SanDI GG'GwC_CC
Cuts at: 854 854
Size: 5231
SapI GCTCTTCn'nnn_
Cuts at: 1873 1951 3254 3464 1873
Size: 78 1303 210 3640
Fragments arranged by size:
3640 1303 210 78
SbfI CC_TGCA'GG
Cuts at: 2278 2278
Size: 5231
ScaI AGT'ACT
Cuts at: 4748 4748
Size: 5231
SfcI C'TryA_G
Cuts at: 33 1219 1582 1611 2088 2274 2959 3952 4143
Size: 1186 363 29 477 186 685 993 191
Cuts at: 4143 33
Size: 1121
Fragments arranged by size:
1186 1121 993 685 477 363 191 186
29
SfoI GGC'GCC
Cuts at: 2663 2911 2663
Size: 248 4983
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5231
SmaI CCC'GGG
Cuts at: 2205 2253 2205
Size: 48 5183
SmlI C'TyrA_G
Cuts at: 440 886 1017 2386 3301 3793 4055 440
Size: 446 131 1369 915 492 262 1616
Fragments arranged by size:
1616 1369 915 492 446 262 131
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5231
SphI G_CATG'C
Cuts at: 2284 2784 2284
Size: 500 4731
StuI AGG'CCT
Cuts at: 1336 1336
Size: 5231
StyI C'CwwG_G
Cuts at: 1406 1457 2365 2719 1406
Size: 51 908 354 3918
Fragments arranged by size:
3918 908 354 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2814 2947 3406 3615 2814
Size: 133 459 209 4430
Fragments arranged by size:
4430 459 209 133
TatI w'GTAC_w
Cuts at: 1444 4746 1444
Size: 3302 1929
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1159 1191 4748 4780 1159
Size: 32 3557 32 1610
Fragments arranged by size:
3557 1610 32 32
Tth111I GACn'n_nGTC
Cuts at: 3028 3028
Size: 5231
XbaI T'CTAG_A
Cuts at: 2262 2262
Size: 5231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2726 2726
Size: 5231
XmaI C'CCGG_G
Cuts at: 2203 2251 2203
Size: 48 5183
XmnI GAAnn'nnTTC
Cuts at: 1571 1720 1945 2176 1571
Size: 149 225 231 4626
Fragments arranged by size:
4626 231 225 149
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AleI AloI ApaI AscI AvrII BaeI
BbvCI BglI BmtI Bpu10I BsiWI BssHII BstEII BstZ17I
ClaI EcoNI FseI FspAI HindIII MfeI NcoI NdeI
NheI NotI NruI PacI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI