Restriction map for
pF1KB6194
(Linear) (Six-base) MAPSORT of: KIBB6194.seq Check: 7785 from: 1 to: 2118
KIBB6194.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 707 1502 2118
Size: 707 795 616
Fragments arranged by size:
795 707 616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 915 1534 2118
Size: 887 28 619 584
Fragments arranged by size:
887 619 584 28
AfeI AGC'GCT
Cuts at: 0 872 2118
Size: 872 1246
AflII C'TTAA_G
Cuts at: 0 909 2118
Size: 909 1209
AflIII A'CryG_T
Cuts at: 0 1328 1586 2118
Size: 1328 258 532
Fragments arranged by size:
1328 532 258
AhdI GACnn_n'nnGTC
Cuts at: 0 42 1686 2118
Size: 42 1644 432
Fragments arranged by size:
1644 432 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 820 2118
Size: 820 1298
AlwNI CAG_nnn'CTG
Cuts at: 0 1084 2118
Size: 1084 1034
ApaLI G'TGCA_C
Cuts at: 0 624 1269 2118
Size: 624 645 849
Fragments arranged by size:
849 645 624
ApoI r'AATT_y
Cuts at: 0 397 1192 1999 2118
Size: 397 795 807 119
Fragments arranged by size:
807 795 397 119
AvaI C'yCGr_G
Cuts at: 0 2095 2118
Size: 2095 23
BanI G'GyrC_C
Cuts at: 0 439 2118
Size: 439 1679
BanII G_rGCy'C
Cuts at: 0 266 724 1760 2118
Size: 266 458 1036 358
Fragments arranged by size:
1036 458 358 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 1173 2118
Size: 310 863 945
Fragments arranged by size:
945 863 310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 469 503 1971 2005 2118
Size: 469 34 1468 34 113
Fragments arranged by size:
1468 469 113 34 34
BclI T'GATC_A
Cuts at: 0 2045 2118
Size: 2045 73
BfrBI ATG'CAT
Cuts at: 0 790 2118
Size: 790 1328
BglII A'GATC_T
Cuts at: 0 926 1646 2118
Size: 926 720 472
Fragments arranged by size:
926 720 472
Bme1580I G_kGCm'C
Cuts at: 0 628 1273 2118
Size: 628 645 845
Fragments arranged by size:
845 645 628
BmgBI CAC'GTC
Cuts at: 0 468 2118
Size: 468 1650
BmrI ACTGGGnnnn_n'
Cuts at: 0 1923 2118
Size: 1923 195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 285 2118
Size: 285 1833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1825 2118
Size: 1825 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 763 2118
Size: 317 446 1355
Fragments arranged by size:
1355 446 317
BsaI GGTCTCn'nnnn_
Cuts at: 0 961 2118
Size: 961 1157
BsaBI GATnn'nnATC
Cuts at: 0 133 2118
Size: 133 1985
BsaWI w'CCGG_w
Cuts at: 0 942 2118
Size: 942 1176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1030 1060 1300 1330 1573 1603 2118
Size: 1030 30 240 30 243 30 515
Fragments arranged by size:
1030 515 243 240 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1765 1768 2118
Size: 1765 3 350
Fragments arranged by size:
1765 350 3
BseYI C'CCAG_C
Cuts at: 0 1653 1863 2118
Size: 1653 210 255
Fragments arranged by size:
1653 255 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 2118
Size: 306 1812
BsiEI CG_ry'CG
Cuts at: 0 1957 2118
Size: 1957 161
BsiHKAI G_wGCw'C
Cuts at: 0 95 266 628 892 1273 1760 2043 2118
Size: 95 171 362 264 381 487 283 75
Fragments arranged by size:
487 381 362 283 264 171 95 75
BsmI GAATG_Cn'
Cuts at: 0 347 357 2118
Size: 347 10 1761
Fragments arranged by size:
1761 347 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 397 2118
Size: 397 1721
Bsp1286I G_dGCh'C
Cuts at: 0 95 266 628 724 892 1273 1760 2043
Size: 95 171 362 96 168 381 487 283
Cuts at: 2043 2118
Size: 75
Fragments arranged by size:
487 381 362 283 171 168 96 95
75
BspHI T'CATG_A
Cuts at: 0 2087 2118
Size: 2087 31
BsrBI CCG'CTC
Cuts at: 0 2106 2118
Size: 2106 12
BsrDI GCAATG_nn'
Cuts at: 0 585 2118
Size: 585 1533
BsrGI T'GTAC_A
Cuts at: 0 1336 2118
Size: 1336 782
BssSI C'ACGA_G
Cuts at: 0 478 529 2118
Size: 478 51 1589
Fragments arranged by size:
1589 478 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1776 2118
Size: 1776 342
BstYI r'GATC_y
Cuts at: 0 926 1646 2118
Size: 926 720 472
Fragments arranged by size:
926 720 472
Bsu36I CC'TnA_GG
Cuts at: 0 1022 2118
Size: 1022 1096
BtgI C'CryG_G
Cuts at: 0 257 1737 1795 2118
Size: 257 1480 58 323
Fragments arranged by size:
1480 323 257 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1620 2118
Size: 1620 498
BtsI GCAGTG_nn'
Cuts at: 0 485 2118
Size: 485 1633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 822 857 2118
Size: 822 35 1261
Fragments arranged by size:
1261 822 35
DraI TTT'AAA
Cuts at: 0 702 2118
Size: 702 1416
DraIII CAC_nnn'GTG
Cuts at: 0 1677 2118
Size: 1677 441
EaeI y'GGCC_r
Cuts at: 0 830 875 1954 2118
Size: 830 45 1079 164
Fragments arranged by size:
1079 830 164 45
EagI C'GGCC_G
Cuts at: 0 1954 2118
Size: 1954 164
EarI CTCTTCn'nnn_
Cuts at: 0 195 1279 1765 1843 1852 2118
Size: 195 1084 486 78 9 266
Fragments arranged by size:
1084 486 266 195 78 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1873 2118
Size: 1873 245
EcoICRI GAG'CTC
Cuts at: 0 264 1758 2118
Size: 264 1494 360
Fragments arranged by size:
1494 360 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 915 1534 1825 2118
Size: 887 28 619 291 293
Fragments arranged by size:
887 619 293 291 28
EcoO109I rG'GnC_Cy
Cuts at: 0 44 543 746 1509 1821 2118
Size: 44 499 203 763 312 297
Fragments arranged by size:
763 499 312 297 203 44
EcoRI G'AATT_C
Cuts at: 0 1192 1999 2118
Size: 1192 807 119
Fragments arranged by size:
1192 807 119
EcoRV GAT'ATC
Cuts at: 0 2114 2118
Size: 2114 4
HaeII r_GCGC'y
Cuts at: 0 435 874 2118
Size: 435 439 1244
Fragments arranged by size:
1244 439 435
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 229 261 623 655 689 721
Size: 28 32 169 32 362 32 34 32
Cuts at: 721 1672 1704 2030 2062 2118
Size: 951 32 326 32 56
Fragments arranged by size:
951 362 326 169 56 34 32 32
32 32 32 32 28
HincII GTy'rAC
Cuts at: 0 75 2118
Size: 75 2043
HpaI GTT'AAC
Cuts at: 0 75 2118
Size: 75 2043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1028 2118
Size: 53 975 1090
Fragments arranged by size:
1090 975 53
MscI TGG'CCA
Cuts at: 0 832 877 2118
Size: 832 45 1241
Fragments arranged by size:
1241 832 45
MslI CAynn'nnrTG
Cuts at: 0 802 1774 2118
Size: 802 972 344
Fragments arranged by size:
972 802 344
NsiI A_TGCA'T
Cuts at: 0 792 2118
Size: 792 1326
NspI r_CATG'y
Cuts at: 0 690 794 1332 1590 2118
Size: 690 104 538 258 528
Fragments arranged by size:
690 538 528 258 104
PasI CC'CwG_GG
Cuts at: 0 164 2118
Size: 164 1954
PciI A'CATG_T
Cuts at: 0 1328 1586 2118
Size: 1328 258 532
Fragments arranged by size:
1328 532 258
PflMI CCAn_nnn'nTGG
Cuts at: 0 1677 2118
Size: 1677 441
PpuMI rG'GwC_Cy
Cuts at: 0 44 746 1509 1821 2118
Size: 44 702 763 312 297
Fragments arranged by size:
763 702 312 297 44
PstI C_TGCA'G
Cuts at: 0 1115 1478 1984 2118
Size: 1115 363 506 134
Fragments arranged by size:
1115 506 363 134
SacI G_AGCT'C
Cuts at: 0 266 1760 2118
Size: 266 1494 358
Fragments arranged by size:
1494 358 266
SanDI GG'GwC_CC
Cuts at: 0 746 2118
Size: 746 1372
SapI GCTCTTCn'nnn_
Cuts at: 0 1765 1843 2118
Size: 1765 78 275
Fragments arranged by size:
1765 275 78
SfcI C'TryA_G
Cuts at: 0 1111 1474 1503 1980 2118
Size: 1111 363 29 477 138
Fragments arranged by size:
1111 477 363 138 29
SmaI CCC'GGG
Cuts at: 0 2097 2118
Size: 2097 21
SmlI C'TyrA_G
Cuts at: 0 332 778 909 2118
Size: 332 446 131 1209
Fragments arranged by size:
1209 446 332 131
StuI AGG'CCT
Cuts at: 0 1228 2118
Size: 1228 890
StyI C'CwwG_G
Cuts at: 0 1298 1349 2118
Size: 1298 51 769
Fragments arranged by size:
1298 769 51
TatI w'GTAC_w
Cuts at: 0 1336 2118
Size: 1336 782
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 2118
Size: 1051 32 1035
Fragments arranged by size:
1051 1035 32
XmaI C'CCGG_G
Cuts at: 0 2095 2118
Size: 2095 23
XmnI GAAnn'nnTTC
Cuts at: 0 1463 1612 1837 2068 2118
Size: 1463 149 225 231 50
Fragments arranged by size:
1463 231 225 149 50
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AhdI AlfI AlwNI
ApaLI ApoI AvaI BanI BanII BbsI BcgI BclI
BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrGI
BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII HpaI MmeI
MscI MslI NsiI NspI PasI PciI PflMI PpuMI
PstI SacI SanDI SapI SfcI SmaI SmlI StuI
StyI TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AleI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BciVI BglI BlpI BmtI Bpu10I BsaAI BsaHI BsiWI
BspEI BspMI BsrFI BssHII BstAPI BstBI BstEII BstZ17I
ClaI DrdI EcoNI FalI FseI FspI FspAI HindIII
KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XcmI XhoI ZraI