Restriction map for pF1KB6188
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcsI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BamHI       BbrPI       BciVI       BfrI        
BlnI        BsaAI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MluNI       MroI        MscI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AclI          448  AACGTT               AA^CGTT
AcyI         1447  GGCGCC               G[GA]^CG[CT]C
AfaI         1078  GTAC                 GT^AC
AflIII         40  ACGCGT               A^C[GA][CT]GT
AlwI         1587  GATCC                GGATC(4/5)
AvaI         1905  CCCGGG               C^[CT]CG[GA]G
BbeI         1447  GGCGCC               GGCGC^C
BbvCI         297  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1704  GCAACTATATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          995  TGATCA               T^GATCA
BglII         249  AGATCT               A^GATCT
BsaHI        1447  GGCGCC               G[GA]^CG[CT]C
BsmBI         316  CGTCTC               CGTCTC(1/5)
BsoBI        1905  CCCGGG               C^[CT]CG[GA]G
BsrBI         926  CCGCTC               CCGCTC(-3/-3)
BstXI        1662  CCAACCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI          122  TGGCCG               [CT]^GGCC[GA]
EcoNI         846  CCTCATCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          995  TGATCA               T^GATCA
Hin1I        1447  GGCGCC               G[GA]^CG[CT]C
HincII        520  GTCAAC               GT[CT]^[GA]AC
HindII        520  GTCAAC               GT[CT]^[GA]AC
KasI         1447  GGCGCC               G^GCGCC
MaeIII       1635  GTCAC                ^GT[ACGT]AC
MfeI         1751  CAATTG               C^AATTG
MluI           40  ACGCGT               A^CGCGT
MseI         1646  TTAA                 T^TAA
MslI         1953  CACCAGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1699  CAGCTG               C[AC]G^C[GT]G
MunI         1751  CAATTG               C^AATTG
NarI         1447  GGCGCC               GG^CGCC
Psp1406I      448  AACGTT               AA^CGTT
PvuII        1699  CAGCTG               CAG^CTG
RsaI         1078  GTAC                 GT^AC
SacI         1159  GAGCTC               GAGCT^C
ScaI         1077  AGTACT               AGT^ACT
SfiI         2067  GGCCCACCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1447  GGCGCC               GGC^GCC
SmaI         1905  CCCGGG               CCC^GGG
SmlI         1162  CTCAAG               C^T[CT][GA]AG
Tru9I        1646  TTAA                 T^TAA
Tsp45I       1635  GTCAC                ^GT[GC]AC
Tsp509I      1752  AATT                 ^AATT
XcmI         1844  CCAAGATGTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1905  CCCGGG               C^CCGGG
XmaI         1905  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccII          41  CGCG                 CG^CG
              314  CGCG                
AlwNI        1051  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2048  CAGTCTCTG           
AspHI         361  GTGCTC               G[AT]GC[AT]^C
             1159  GAGCTC              
BanI          387  GGCACC               G^G[CT][GA]CC
             1447  GGCGCC              
BfaI          112  CTAG                 C^TAG
             1474  CTAG                
BlpI           18  GCTCAGC              GC^T[ACGT]AGC
               63  GCTTAGC             
Bpu1102I       18  GCTCAGC              GC^T[ACGT]AGC
               63  GCTTAGC             
BsaBI        1926  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1962  GATTCACATC          
BsaI         1469  GAGACC               GGTCTC(1/5)
             1882  GAGACC              
BsiHKAI       361  GTGCTC               G[AT]GC[AT]^C
             1159  GAGCTC              
BsmI         1110  GAATGC               GAATGC(1/-1)
             1738  GCATTC              
BspMI          59  ACCTGC               ACCTGC(4/8)
             1802  ACCTGC              
BsrDI        1218  CATTGC               GCAATG(2/0)
             2024  GCAATG              
BsrFI         882  GCCGGC               [GA]^CCGG[CT]
             1502  GCCGGC              
BstUI          41  CGCG                 CG^CG
              314  CGCG                
BstYI         249  AGATCT               [GA]^GATC[CT]
             1586  AGATCC              
CelII          18  GCTCAGC              GC^T[ACGT]AGC
               63  GCTTAGC             
CfoI         1448  GCGC                 GCG^C
             1563  GCGC                
Cfr10I        882  GCCGGC               [GA]^CCGG[CT]
             1502  GCCGGC              
FauI          925  CCCGC                CCCGC(4/6)
              951  CCCGC               
HaeII        1447  GGCGCC               [GA]GCGC^[CT]
             1562  AGCGCC              
HhaI         1448  GCGC                 GCG^C
             1563  GCGC                
HinP1I       1448  GCGC                 G^CGC
             1563  GCGC                
MaeI          112  CTAG                 C^TAG
             1474  CTAG                
MaeII         191  ACGT                 A^CGT
              449  ACGT                
MamI         1926  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1962  GATTCACATC          
MflI          249  AGATCT               [GA]^GATC[CT]
             1586  AGATCC              
MvnI           41  CGCG                 CG^CG
              314  CGCG                
NaeI          882  GCCGGC               GCC^GGC
             1502  GCCGGC              
NcoI          223  CCATGG               C^CATGG
             1868  CCATGG              
NgoMIV        882  GCCGGC               G^CCGGC
             1502  GCCGGC              
PpuMI         581  AGGTCCT              [GA]G^G[AT]CC[CT]
             1366  GGGTCCT             
PshAI          76  GACTGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1610  GACAGAAGTC          
StuI          530  AGGCCT               AGG^CCT
             2075  AGGCCT              
TaqI          864  TCGA                 T^CGA
             1934  TCGA                
TthHB8I       864  TCGA                 T^CGA
             1934  TCGA                
XhoII         249  AGATCT               [GA]^GATC[CT]
             1586  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AluI        AocI        ApaI        AspI        
AvaII       BanII       BbsI        BbvI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Bsu36I      Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HinfI       HpaII       HphI        
ItaI        Ksp632I     MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflFI       PleI        PstI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        TseI        TspRI       
Tth111I     
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