Restriction map for pF1KB6188
 (Circular) (Six-base) MAPSORT of: pF1KB6188.seq  Check: 1331  from: 1  to: 5222



pF1KB6188  5222 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1919    1919 
   Size:       5222
 
AccI GT'mk_AC

Cuts at:   2260    2260 
   Size:       5222
 
Acc65I G'GTAC_C

Cuts at:   2238    2238 
   Size:       5222
 
AclI AA'CG_TT

Cuts at:    557    5062     557 
   Size:       4505     717
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    346     707    1004    1085    1105    1352    3047    3479    4226 
   Size:        361     297      81      20     247    1695     432     747

Cuts at:   4226    4494     346 
   Size:        268    1074

  Fragments arranged by size: 

                1695    1074     747     432     361     297     268     247
                  81      20
 
AflIII A'CryG_T

Cuts at:    148    2574    3678    4655     148 
   Size:       2426    1104     977     715

  Fragments arranged by size: 

                2426    1104     977     715
 
AgeI A'CCGG_T

Cuts at:   3610    3610 
   Size:       5222
 
AleI CACnn'nnGTG

Cuts at:   2065    2065 
   Size:       5222
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    832     832 
   Size:       5222
 
AlwNI CAG_nnn'CTG

Cuts at:   1164    2161    2527    4094    1164 
   Size:        997     366    1567    2292

  Fragments arranged by size: 

                2292    1567     997     366
 
ApaI G_GGCC'C

Cuts at:    787    1489    1710    2013     787 
   Size:        702     221     303    3996

  Fragments arranged by size: 

                3996     702     303     221
 
ApaLI G'TGCA_C

Cuts at:   3992    3992 
   Size:       5222
 
ApoI r'AATT_y

Cuts at:   2226    4575    4831    2226 
   Size:       2349     256    2617

  Fragments arranged by size: 

                2617    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5222
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5222
 
AvaI C'yCGr_G

Cuts at:   2013    2242    2013 
   Size:        229    4993
 
BamHI G'GATC_C

Cuts at:   2247    2247 
   Size:       5222
 
BanI G'GyrC_C

Cuts at:    495    1555    2238    2652    2900    2935     495 
   Size:       1060     683     414     248      35    2782

  Fragments arranged by size: 

                2782    1060     683     414     248      35
 
BanII G_rGCy'C

Cuts at:    329     787    1271    1489    1499    1710    2013    2236    3266 
   Size:        458     484     218      10     211     303     223    1030

Cuts at:   3266     329 
   Size:       2285

  Fragments arranged by size: 

                2285    1030     484     458     303     223     218     211
                  10
 
BbeI G_GCGC'C

Cuts at:   1559    2656    2904    1559 
   Size:       1097     248    3877

  Fragments arranged by size: 

                3877    1097     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    487     709    1305    1880     487 
   Size:        222     596     575    3829

  Fragments arranged by size: 

                3829     596     575     222
 
BbvCI CC'TCA_GC

Cuts at:    406     406 
   Size:       5222
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1801    1835    1801 
   Size:         34    5188
 
BciVI GTATCCnnnnn_n'

Cuts at:   3120    3881    5222    3120 
   Size:        761    1341    3120

  Fragments arranged by size: 

                3120    1341     761
 
BclI T'GATC_A

Cuts at:   1103    2742    1103 
   Size:       1639    3583
 
BglI GCCn_nnn'nGGC

Cuts at:    987    1252    2173    2182     987 
   Size:        265     921       9    4027

  Fragments arranged by size: 

                4027     921     265       9
 
BglII A'GATC_T

Cuts at:    357    2737    4774     357 
   Size:       2380    2037     805

  Fragments arranged by size: 

                2380    2037     805
 
BlpI GC'TnA_GC

Cuts at:    127     172    2334     127 
   Size:         45    2162    3015

  Fragments arranged by size: 

                3015    2162      45
 
Bme1580I G_kGCm'C

Cuts at:    787    1489    1710    2013    2096    2847    2940    3996     787 
   Size:        702     221     303      83     751      93    1056    2013

  Fragments arranged by size: 

                2013    1056     751     702     303     221      93      83
            
 
BmgBI CAC'GTC

Cuts at:    300     300 
   Size:       5222
 
BmrI ACTGGGnnnn_n'

Cuts at:    195     931     940    1517    2434    2515    2850    4994     195 
   Size:        736       9     577     917      81     335    2144     423

  Fragments arranged by size: 

                2144     917     736     577     423     335      81       9
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3589    3589 
   Size:       5222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    648    1186    1261    1453    1733     648 
   Size:        538      75     192     280    4137

  Fragments arranged by size: 

                4137     538     280     192      75
 
Bpu10I CC'TnA_GC

Cuts at:    406     406 
   Size:       5222
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1255    2398    3277    3769    4067    1255 
   Size:       1143     879     492     298    2410

  Fragments arranged by size: 

                2410    1143     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1571    1984    4926    1571 
   Size:        413    2942    1867

  Fragments arranged by size: 

                2942    1867     413
 
BsaAI yAC'GTr

Cuts at:   2575    2575 
   Size:       5222
 
BsaBI GATnn'nnATC

Cuts at:      6    2038    2074    2736    4773       6 
   Size:       2032      36     662    2037     455

  Fragments arranged by size: 

                2037    2032     662     455      36
 
BsaHI Gr'CG_yC

Cuts at:   1556    2653    2901    3603    5099    1556 
   Size:       1097     248     702    1496    1679

  Fragments arranged by size: 

                1679    1496    1097     702     248
 
BsaWI w'CCGG_w

Cuts at:   2411    2932    3610    3884    4031    4442    2411 
   Size:        521     678     274     147     411    3191

  Fragments arranged by size: 

                3191     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    274     304     494     524     674     704    1927    1957    2170 
   Size:         30     190      30     150      30    1223      30     213

Cuts at:   2170    2200     274 
   Size:         30    3296

  Fragments arranged by size: 

                3296    1223     213     190     150      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    250     390     651     700     792    1373    1590    2041     250 
   Size:        140     261      49      92     581     217     451    3431

  Fragments arranged by size: 

                3431     581     451     261     217     140      92      49
            
 
BseYI C'CCAG_C

Cuts at:    747     895    1425    1499    1504    1661    1920    2647    3405 
   Size:        148     530      74       5     157     259     727     758

Cuts at:   3405    3982    4478     747 
   Size:        577     496    1491

  Fragments arranged by size: 

                1491     758     727     577     530     496     259     157
                 148      74       5
 
BsiEI CG_ry'CG

Cuts at:    104    2764    2810    4018     104 
   Size:       2660      46    1208    1308

  Fragments arranged by size: 

                2660    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    473    1271    2236    3014    3204    3996     473 
   Size:        798     965     778     190     792    1699

  Fragments arranged by size: 

                1699     965     798     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1224    1846    1224 
   Size:        622    4600
 
BsmBI CGTCTCn'nnnn_

Cuts at:    430     430 
   Size:       5222
 
Bsp1286I G_dGCh'C

Cuts at:    329     473     787    1271    1489    1499    1710    2013    2096 
   Size:        144     314     484     218      10     211     303      83

Cuts at:   2096    2236    2847    2940    3014    3204    3266    3996     329 
   Size:        140     611      93      74     190      62     730    1555

  Fragments arranged by size: 

                1555     730     611     484     314     303     218     211
                 190     144     140      93      83      74      62      10
            
 
BspEI T'CCGG_A

Cuts at:   4442    4442 
   Size:       5222
 
BspHI T'CATG_A

Cuts at:   4398    4398 
   Size:       5222
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    176    1919    2272    2486    2788    3169     176 
   Size:       1743     353     214     302     381    2229

  Fragments arranged by size: 

                2229    1743     381     353     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1036    3514    3568    5052    5217      50 
   Size:        986    2478      54    1484     165      55

  Fragments arranged by size: 

                2478    1484     986     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1325    2139    3134    1325 
   Size:        814     995    3413

  Fragments arranged by size: 

                3413     995     814
 
BsrFI r'CCGG_y

Cuts at:    990    1610    3220    3401    3610     990 
   Size:        620    1610     181     209    2602

  Fragments arranged by size: 

                2602    1610     620     209     181
 
BssSI C'ACGA_G

Cuts at:   3493    3851    3493 
   Size:        358    4864
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2599    2599 
   Size:       5222
 
BstBI TT'CG_AA

Cuts at:   3583    3583 
   Size:       5222
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1777    1777 
   Size:       5222
 
BstYI r'GATC_y

Cuts at:      1     357    1694    2247    2714    2737    3072    3318    4330 
   Size:        356    1337     553     467      23     335     246    1012

Cuts at:   4330    4416    4428    4774       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1337    1012     553     467     449     356     346     335
                 246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    804    1490    1636     804 
   Size:        686     146    4390

  Fragments arranged by size: 

                4390     686     146
 
BtgI C'CryG_G

Cuts at:    107     331    1006    1732    1976    2004     107 
   Size:        224     675     726     244      28    3325

  Fragments arranged by size: 

                3325     726     675     244     224      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    404    3172    3353    3516     404 
   Size:       2768     181     163    2110

  Fragments arranged by size: 

                2768    2110     181     163
 
BtsI GCAGTG_nn'

Cuts at:    835    2465     835 
   Size:       1630    3592
 
DraI TTT'AAA

Cuts at:   2221    2221 
   Size:       5222
 
DrdI GACnn_nn'nnGTC

Cuts at:   2928    3786    2928 
   Size:        858    4364
 
EaeI y'GGCC_r

Cuts at:    230    2807    2981    3372    3399    4744     230 
   Size:       2577     174     391      27    1345     708

  Fragments arranged by size: 

                2577    1345     708     391     174      27
 
EagI C'GGCC_G

Cuts at:   2807    2807 
   Size:       5222
 
EarI CTCTTCn'nnn_

Cuts at:    256     399    1015    1327    1597    3245    3455     256 
   Size:        143     616     312     270    1648     210    2023

  Fragments arranged by size: 

                2023    1648     616     312     270     210     143
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    1198    3430    3740    3886    5032     127 
   Size:       1071    2232     310     146    1146     317

  Fragments arranged by size: 

                2232    1146    1071     317     310     146
 
EcoICRI GAG'CTC

Cuts at:   1269    2234    1269 
   Size:        965    4257
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    346     648     707    1004    1085    1105    1186    1261    1352 
   Size:        302      59     297      81      20      81      75      91

Cuts at:   1352    1453    1733    3047    3479    4226    4494     346 
   Size:        101     280    1314     432     747     268    1074

  Fragments arranged by size: 

                1314    1074     747     432     302     297     280     268
                 101      91      81      81      75      59      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:    958     958 
   Size:       5222
 
EcoO109I rG'GnC_Cy

Cuts at:    690    1245    1475    1485    1486    1706    2009    2010    2361 
   Size:        555     230      10       1     220     303       1     351

Cuts at:   2361     690 
   Size:       3551

  Fragments arranged by size: 

                3551     555     351     303     230     220      10       1
                   1
 
EcoRI G'AATT_C

Cuts at:   2226    2226 
   Size:       5222
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3241    3241 
   Size:       5222
 
FspI TGC'GCA

Cuts at:   3003    3003 
   Size:       5222
 
HaeII r_GCGC'y

Cuts at:   1559    1674    2656    2904    3926    4905    1559 
   Size:        115     982     248    1022     979    1876

  Fragments arranged by size: 

                1876    1022     982     979     248     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    274     306     514     546     565     597     674     700     706 
   Size:         32     208      32      19      32      77      26       6

Cuts at:    706     732     787     819    1981    2013    3064    3094    3096 
   Size:         26      55      32    1162      32    1051      30       2

Cuts at:   3096    3126     274 
   Size:         30    2370

  Fragments arranged by size: 

                2370    1162    1051     208      77      55      32      32
                  32      32      32      30      30      26      26      19
                   6       2
 
HincII GTy'rAC

Cuts at:    630    2261     630 
   Size:       1631    3591
 
KasI G'GCGC_C

Cuts at:   1555    2652    2900    1555 
   Size:       1097     248    3877

  Fragments arranged by size: 

                3877    1097     248
 
KpnI G_GTAC'C

Cuts at:   2242    2242 
   Size:       5222
 
MfeI C'AATT_G

Cuts at:   1859    1859 
   Size:       5222
 
MluI A'CGCG_T

Cuts at:    148    4655     148 
   Size:       4507     715
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1794    3893    4077    1794 
   Size:       2099     184    2939

  Fragments arranged by size: 

                2939    2099     184
 
MscI TGG'CCA

Cuts at:   2983    2983 
   Size:       5222
 
MslI CAynn'nnrTG

Cuts at:   2065    3211    3338    2065 
   Size:       1146     127    3949

  Fragments arranged by size: 

                3949    1146     127
 
MspA1I CmG'CkG

Cuts at:   1809    2333    2647    3007    4020    4265    1809 
   Size:        524     314     360    1013     245    2766

  Fragments arranged by size: 

                2766    1013     524     360     314     245
 
NaeI GCC'GGC

Cuts at:    992    1612    3403     992 
   Size:        620    1791    2811

  Fragments arranged by size: 

                2811    1791     620
 
NarI GG'CG_CC

Cuts at:   1556    2653    2901    1556 
   Size:       1097     248    3877

  Fragments arranged by size: 

                3877    1097     248
 
NcoI C'CATG_G

Cuts at:    107     331    1976     107 
   Size:        224    1645    3353

  Fragments arranged by size: 

                3353    1645     224
 
NgoMIV G'CCGG_C

Cuts at:    990    1610    3401     990 
   Size:        620    1791    2811

  Fragments arranged by size: 

                2811    1791     620
 
NspI r_CATG'y

Cuts at:   2275    2775    3682    2275 
   Size:        500     907    3815

  Fragments arranged by size: 

                3815     907     500
 
PasI CC'CwG_GG

Cuts at:   1482    1549    1482 
   Size:         67    5155
 
PciI A'CATG_T

Cuts at:   3678    3678 
   Size:       5222
 
PfoI T'CCnGG_A

Cuts at:    692    1132     692 
   Size:        440    4782
 
PmeI GTTT'AAAC

Cuts at:   2221    2221 
   Size:       5222
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4361    4393    4361 
   Size:         32    5190
 
PpuMI rG'GwC_Cy

Cuts at:    690    1475     690 
   Size:        785    4437
 
PshAI GACnn'nnGTC

Cuts at:    188    1722     188 
   Size:       1534    3688
 
PspOMI G'GGCC_C

Cuts at:    783    1485    1706    2009     783 
   Size:        702     221     303    3996

  Fragments arranged by size: 

                3996     702     303     221
 
PstI C_TGCA'G

Cuts at:    197     842     917    1160    2166    2269    2954     197 
   Size:        645      75     243    1006     103     685    2465

  Fragments arranged by size: 

                2465    1006     685     645     243     103      75
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5222
 
PvuII CAG'CTG

Cuts at:   1809    2647    3007    1809 
   Size:        838     360    4024

  Fragments arranged by size: 

                4024     838     360
 
RsrII CG'GwC_CG

Cuts at:   3417    3417 
   Size:       5222
 
SacI G_AGCT'C

Cuts at:   1271    2236    1271 
   Size:        965    4257
 
SalI G'TCGA_C

Cuts at:   2259    2259 
   Size:       5222
 
SapI GCTCTTCn'nnn_

Cuts at:    256     399    1015    1597    3245    3455     256 
   Size:        143     616     582    1648     210    2023

  Fragments arranged by size: 

                2023    1648     616     582     210     143
 
SbfI CC_TGCA'GG

Cuts at:   2269    2269 
   Size:       5222
 
ScaI AGT'ACT

Cuts at:   1187    4739    1187 
   Size:       3552    1670
 
SfcI C'TryA_G

Cuts at:     33     193     838     851     913    1156    2162    2265    2950 
   Size:        160     645      13      62     243    1006     103     685

Cuts at:   2950    3943    4134      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121    1006     993     685     645     243     191     160
                 103      62      13
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2182    2182 
   Size:       5222
 
SfoI GGC'GCC

Cuts at:   1557    2654    2902    1557 
   Size:       1097     248    3877

  Fragments arranged by size: 

                3877    1097     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5222
 
SmaI CCC'GGG

Cuts at:   2015    2244    2015 
   Size:        229    4993
 
SmlI C'TyrA_G

Cuts at:   1270    2377    3292    3784    4046    1270 
   Size:       1107     915     492     262    2446

  Fragments arranged by size: 

                2446    1107     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5222
 
SphI G_CATG'C

Cuts at:   2275    2775    2275 
   Size:        500    4722
 
StuI AGG'CCT

Cuts at:    640    2185     640 
   Size:       1545    3677
 
StyI C'CwwG_G

Cuts at:    107     331     491    1559    1976    2356    2710     107 
   Size:        224     160    1068     417     380     354    2619

  Fragments arranged by size: 

                2619    1068     417     380     354     224     160
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1841    1880    2805    2938    3397    3606    1841 
   Size:         39     925     133     459     209    3457

  Fragments arranged by size: 

                3457     925     459     209     133      39
 
TatI w'GTAC_w

Cuts at:   1185    4737    1185 
   Size:       3552    1670
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    494     526    2170    2202    4739    4771     494 
   Size:         32    1644      32    2537      32     945

  Fragments arranged by size: 

                2537    1644     945      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    486     708    1995    3019     486 
   Size:        222    1287    1024    2689

  Fragments arranged by size: 

                2689    1287    1024     222
 
XbaI T'CTAG_A

Cuts at:   2253    2253 
   Size:       5222
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1959    2717    1959 
   Size:        758    4464
 
XmaI C'CCGG_G

Cuts at:   2013    2242    2013 
   Size:        229    4993
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI     PfoI     PmeI
     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AloI     AscI    AvrII     BaeI
    BfrBI     BmtI     BsgI    BsiWI    BsrGI   BssHII   BstEII  BstZ17I
     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI
     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI     PmlI
     PsiI    PspXI     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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