Restriction map for pF1KB6183
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AccII       AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AviII       AvrII       BcgI        BciVI       
BclI        BfrI        BlnI        BsaBI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspLU11I    BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        EagI        EclXI       Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MseI        MunI        MvnI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         549  GACGTC               GACGT^C
AccI           89  GTCGAC               GT^[AC][GT]AC
AccIII       1922  TCCGGA               T^CCGGA
AhdI          751  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1510  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1112  AGCGCT               AGC^GCT
ApaI           58  GGGCCC               GGGCC^C
AspEI         751  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1835  TGGCCA               TGG^CCA
BamHI         250  GGATCC               G^GATCC
BbeI           40  GGCGCC               GGCGC^C
BfaI          643  CTAG                 C^TAG
BglII        1382  AGATCT               A^GATCT
BseAI        1922  TCCGGA               T^CCGGA
BsmI         1297  GAATGC               GAATGC(1/-1)
BspEI        1922  TCCGGA               T^CCGGA
BspHI        1610  TCATGA               T^CATGA
BsrBI        1254  CCGCTC               CCGCTC(-3/-3)
Bsu36I       1510  CCTGAGG              CC^T[ACGT]AGG
DraIII       1126  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           67  GACCAGGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      751  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1112  AGCGCT               AGC^GCT
Eco81I       1510  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1305  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII         89  GTCGAC               GT[CT]^[GA]AC
HindII         89  GTCGAC               GT[CT]^[GA]AC
KasI           40  GGCGCC               G^GCGCC
MaeI          643  CTAG                 C^TAG
MluNI        1835  TGGCCA               TGG^CCA
MroI         1922  TCCGGA               T^CCGGA
MscI         1835  TGGCCA               TGG^CCA
NarI           40  GGCGCC               GG^CGCC
PpuMI         790  GGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1610  TCATGA               T^CATGA
SalI           89  GTCGAC               G^TCGAC
SfoI           40  GGCGCC               GGC^GCC
StuI         1850  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

BbrPI        1222  CACGTG               CAC^GTG
             1324  CACGTG              
BbvCI        1777  CCTCAGC              CCTCAGC(-5/-2)
              312  GCTGAGG             
BlpI          829  GCTGAGC              GC^T[ACGT]AGC
             1256  GCTCAGC             
Bpu1102I      829  GCTGAGC              GC^T[ACGT]AGC
             1256  GCTCAGC             
BsaI         1663  GAGACC               GGTCTC(1/5)
             1446  GGTCTC              
BsaWI        1841  ACCGGA               [AT]^CCGG[AT]
             1922  TCCGGA              
BssSI         398  CACGAG               CACGAG(-5/-1)
             1696  CACGAG              
BstAPI        285  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1311  GCAGGCCCTGC         
BstEII       1062  GGTCACC              G^GT[ACGT]ACC
             1809  GGTTACC             
BstPI        1062  GGTCACC              G^GT[ACGT]ACC
             1809  GGTTACC             
BstXI          47  CCACGCCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1265  CCACAGTGCTGG        
CelII         829  GCTGAGC              GC^T[ACGT]AGC
             1256  GCTCAGC             
EcoO65I      1062  GGTCACC              G^GT[ACGT]ACC
             1809  GGTTACC             
EcoT14I      1640  CCTTGG               C^C[AT][AT]GG
             1748  CCAAGG              
HgaI         1182  GACGC                GACGC(5/10)
             1247  GCGTC               
NspI          628  GCATGT               [GA]CATG^[CT]
             1673  ACATGC              
PmaCI        1222  CACGTG               CAC^GTG
             1324  CACGTG              
PmlI         1222  CACGTG               CAC^GTG
             1324  CACGTG              
SacI          217  GAGCTC               GAGCT^C
             1333  GAGCTC              
SapI         1570  GAAGAGC              GCTCTTC(1/4)
             1711  GAAGAGC             
SmaI           30  CCCGGG               CCC^GGG
             1085  CCCGGG              
StyI         1640  CCTTGG               C^C[AT][AT]GG
             1748  CCAAGG              
TaqI           90  TCGA                 T^CGA
              944  TCGA                
TthHB8I        90  TCGA                 T^CGA
              944  TCGA                
XcmI          470  CCAGCTGCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1619  CCATCAGTCTTCTGG     
XmaCI          30  CCCGGG               C^CCGGG
             1085  CCCGGG              
XmaI           30  CCCGGG               C^CCGGG
             1085  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BglI        
BmyI        BpmI        BpuAI       BsaAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TseI        Tsp45I      TspRI       
Van91I      XhoII       
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