Restriction map for pF1KB5669
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiHKAI     BsiWI       
BsmBI       BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstXI       
BstZ17I     Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        Eam1105I    Eco47III    
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT14I     
FbaI        FseI        FspI        HaeII       Hin1I       
HpaI        HphI        KasI        KpnI        MamI        
MfeI        MluI        MroI        MslI        MunI        
NarI        NcoI        NheI        NotI        NruI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccI          589  GTAGAC               GT^[AC][GT]AC
AflIII        503  ACACGT               A^C[GA][CT]GT
AlwNI         337  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          610  CCTCAGG              CC^T[ACGT]AGG
AseI          226  ATTAAT               AT^TAAT
AsnI          226  ATTAAT               AT^TAAT
AvaI          103  CCCGAG               C^[CT]CG[GA]G
BalI          132  TGGCCA               TGG^CCA
BbsI          499  GAAGAC               GAAGAC(2/6)
BbvI           51  GCTGC                GCAGC(8/12)
BcnI          189  CCCGG                CC^[GC]GG
BglI           28  GCCGGCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          256  GCTAAGC              GC^T[ACGT]AGC
BmyI         1011  GGGCAC               G[AGT]GC[ACT]^C
Bpu1102I      256  GCTAAGC              GC^T[ACGT]AGC
BpuAI         499  GAAGAC               GAAGAC(2/6)
BsiEI          67  CGGCCG               CG[GA][CT]^CG
BsmI          923  GAATGC               GAATGC(1/-1)
BsoBI         103  CCCGAG               C^[CT]CG[GA]G
Bsp1286I     1011  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      441  TGTACA               T^GTACA
BsrFI          28  GCCGGC               [GA]^CCGG[CT]
BsrGI         441  TGTACA               T^GTACA
BstBI         920  TTCGAA               TT^CGAA
BstNI         135  CCAGG                CC^[AT]GG
Bsu36I        610  CCTCAGG              CC^T[ACGT]AGG
CelII         256  GCTAAGC              GC^T[ACGT]AGC
CfoI           49  GCGC                 GCG^C
Cfr10I         28  GCCGGC               [GA]^CCGG[CT]
EagI           67  CGGCCG               C^GGCCG
EclXI          67  CGGCCG               C^GGCCG
Eco52I         67  CGGCCG               C^GGCCG
Eco81I        610  CCTCAGG              CC^T[ACGT]AGG
EcoRI         268  GAATTC               G^AATTC
EcoRII        135  CCAGG                ^CC[AT]GG
EcoT22I       964  ATGCAT               ATGCA^T
HgaI           93  GCGTC                GACGC(5/10)
HhaI           49  GCGC                 GCG^C
HinP1I         49  GCGC                 G^CGC
HincII        728  GTCAAC               GT[CT]^[GA]AC
HindII        728  GTCAAC               GT[CT]^[GA]AC
KspI           64  CCGCGG               CCGC^GG
MaeII         505  ACGT                 A^CGT
MluNI         132  TGGCCA               TGG^CCA
MscI          132  TGGCCA               TGG^CCA
MvaI          135  CCAGG                CC^[AT]GG
NaeI           28  GCCGGC               GCC^GGC
NciI          189  CCCGG                CC^[GC]GG
NdeI          788  CATATG               CA^TATG
NgoMIV         28  GCCGGC               G^CCGGC
NsiI          964  ATGCAT               ATGCA^T
NspI          966  GCATGC               [GA]CATG^[CT]
NspV          920  TTCGAA               TT^CGAA
PshBI         226  ATTAAT               AT^TAAT
PvuII         541  CAGCTG               CAG^CTG
SacII          64  CCGCGG               CCGC^GG
SfuI          920  TTCGAA               TT^CGAA
SphI          966  GCATGC               GCATG^C
SspBI         441  TGTACA               T^GTACA
SspI          775  AATATT               AAT^ATT
TaqI          921  TCGA                 T^CGA
TfiI          950  GAATC                G^A[AT]TC
TseI           51  GCTGC                G^C[AT]GC
TthHB8I       921  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          442  GTAC                 GT^AC
              616  GTAC                
BamHI           6  GGATCC               G^GATCC
              148  GGATCC              
BfaI          284  CTAG                 C^TAG
              812  CTAG                
BpmI          165  CTCCAG               CTGGAG(16/14)
               55  CTGGAG              
BsaWI         432  ACCGGA               [AT]^CCGG[AT]
              462  TCCGGT              
BseRI          82  GAGGAG               GAGGAG(10/8)
              287  GAGGAG              
BsmAI         139  GAGAC                GTCTC(1/5)
              312  GAGAC               
BsmFI          95  GTCCC                GGGAC(10/14)
              101  GTCCC               
BssKI         135  CCAGG                ^CC[ACGT]GG
              189  CCCGG               
BstYI           6  GGATCC               [GA]^GATC[CT]
              148  GGATCC              
DsaI           38  CCGTGG               C^C[GA][CT]GG
               64  CCGCGG              
EaeI           67  CGGCCG               [CT]^GGCC[GA]
              132  TGGCCA              
EarI          993  CTCTTC               CTCTTC(1/4)
              309  GAAGAG              
FauI           46  CCCGC                CCCGC(4/6)
               77  GCGGG               
HindIII       259  AAGCTT               A^AGCTT
              274  AAGCTT              
Ksp632I       993  CTCTTC               CTCTTC(1/4)
              309  GAAGAG              
MaeI          284  CTAG                 C^TAG
              812  CTAG                
MaeIII        176  GTGAC                ^GT[ACGT]AC
              185  GTGAC               
MflI            6  GGATCC               [GA]^GATC[CT]
              148  GGATCC              
MspA1I         64  CCGCGG               C[AC]G^C[GT]G
              541  CAGCTG              
NlaIII        445  CATG                 CATG^
              967  CATG                
PleI          892  GACTC                GAGTC(4/5)
               58  GAGTC               
RsaI          442  GTAC                 GT^AC
              616  GTAC                
ScrFI         135  CCAGG                CC^[ACGT]GG
              189  CCCGG               
SfaNI         345  GATGC                GCATC(5/9)
              557  GCATC               
Tsp45I        176  GTGAC                ^GT[GC]AC
              185  GTGAC               
XhoII           6  GGATCC               [GA]^GATC[CT]
              148  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        AlwI        
ApoI        BsaJI       BsiYI       BslI        BsrI        
BstF5I      BstUI       Cac8I       DdeI        DpnII       
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        MboI        MboII       MnlI        
MseI        MspI        MvnI        MwoI        NdeII       
NlaIV       Sau3AI      Tru9I       Tsp509I     TspRI       
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