Restriction map for pF1KB5632
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfrI        BglI        BglII       
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraIII      DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       FbaI        FokI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          183  GTCTAC               GT^[AC][GT]AC
AcsI          474  GAATTT               [GA]^AATT[CT]
AlwI          697  GGATC                GGATC(4/5)
AlwNI         773  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          708  GGGCCC               GGGCC^C
ApoI          474  GAATTT               [GA]^AATT[CT]
AvaII         525  GGTCC                G^G[AT]CC
AvrII         528  CCTAGG               C^CTAGG
BanI          391  GGCACC               G^G[CT][GA]CC
BcgI          639  GCAGGCTCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI           20  CCGGG                CC^[GC]GG
BlnI          528  CCTAGG               C^CTAGG
BseRI         644  CTCCTC               GAGGAG(10/8)
BsmFI         654  GTCCC                GGGAC(10/14)
BspMI         169  ACCTGC               ACCTGC(4/8)
BsrFI          47  GCCGGC               [GA]^CCGG[CT]
BstYI         697  GGATCT               [GA]^GATC[CT]
Cfr10I         47  GCCGGC               [GA]^CCGG[CT]
DpnII         698  GATC                 ^GATC
DraI          307  TTTAAA               TTT^AAA
DraII         707  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI          424  GACATCGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      707  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         793  GATATC               GAT^ATC
EcoT22I       350  ATGCAT               ATGCA^T
FauI          200  GCGGG                CCCGC(4/6)
HgaI            7  GCGTC                GACGC(5/10)
HincII        204  GTTGAC               GT[CT]^[GA]AC
HindII        204  GTTGAC               GT[CT]^[GA]AC
MboI          698  GATC                 ^GATC
MboII         137  TCTTC                GAAGA(8/7)
MflI          697  GGATCT               [GA]^GATC[CT]
NaeI           47  GCCGGC               GCC^GGC
NciI           20  CCGGG                CC^[GC]GG
NdeII         698  GATC                 ^GATC
NgoMIV         47  GCCGGC               G^CCGGC
NlaIII        552  CATG                 CATG^
NsiI          350  ATGCAT               ATGCA^T
PshAI         207  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          606  CTGCAG               CTGCA^G
Sau3AI        698  GATC                 ^GATC
SpeI          187  ACTAGT               A^CTAGT
XhoII         697  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BanII          85  GGGCTC               G[GA]GC[CT]^C
              708  GGGCCC              
BfaI          188  CTAG                 C^TAG
              529  CTAG                
BmyI           85  GGGCTC               G[AGT]GC[ACT]^C
              708  GGGCCC              
BsaI          332  GAGACC               GGTCTC(1/5)
              532  GGTCTC              
BsiYI          14  CCGGAGCCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              228  CCAGCCAGAGG         
BslI           14  CCGGAGCCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              228  CCAGCCAGAGG         
Bsp1286I       85  GGGCTC               G[AGT]GC[ACT]^C
              708  GGGCCC              
CfoI           51  GCGC                 GCG^C
               79  GCGC                
DdeI          210  CTCAG                C^T[ACGT]AG
              668  CTGAG               
HaeII          50  GGCGCT               [GA]GCGC^[CT]
               78  GGCGCT              
HaeIII        240  GGCC                 GG^CC
              709  GGCC                
HhaI           51  GCGC                 GCG^C
               79  GCGC                
HinP1I         51  GCGC                 G^CGC
               79  GCGC                
MaeI          188  CTAG                 C^TAG
              529  CTAG                
MspA1I         95  CAGCTG               C[AC]G^C[GT]G
              321  CAGCTG              
MwoI            4  GCAGCGTCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               25  GCGGTGATTGC         
PleI          684  GAGTC                GAGTC(4/5)
              775  GAGTC               
PvuII          95  CAGCTG               CAG^CTG
              321  CAGCTG              
SfaNI         349  GATGC                GCATC(5/9)
              352  GCATC               
SfcI          570  CTATAG               C^T[GA][CT]AG
              606  CTGCAG              
TfiI          317  GAATC                G^A[AT]TC
              555  GATTC               
Tsp45I        627  GTCAC                ^GT[GC]AC
              755  GTGAC               
Tsp509I       295  AATT                 ^AATT
              475  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BpmI        
BsaJI       BsmAI       BsrI        BssKI       BstNI       
Cac8I       Cfr13I      EcoRII      EcoT14I     Fnu4HI      
HapII       HinfI       HpaII       HphI        ItaI        
MaeIII      MnlI        MseI        MslI        MspI        
MvaI        NlaIV       RsaI        Sau96I      ScrFI       
StyI        Tru9I       TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com