Restriction map for pF1KB5632
 (Linear) (Six-base) MAPSORT of: KIBB5632.seq  Check: 3585  from: 1  to: 807



KIBB5632.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     184     807 
   Size:        184     623
 
AleI CACnn'nnGTG

Cuts at:      0     250     807 
   Size:        250     557
 
AlwNI CAG_nnn'CTG

Cuts at:      0     778     807 
   Size:        778      29
 
ApaI G_GGCC'C

Cuts at:      0     712     807 
   Size:        712      95
 
ApoI r'AATT_y

Cuts at:      0     474     807 
   Size:        474     333
 
AvrII C'CTAG_G

Cuts at:      0     528     807 
   Size:        528     279
 
BanI G'GyrC_C

Cuts at:      0     391     807 
   Size:        391     416
 
BanII G_rGCy'C

Cuts at:      0      89     712     807 
   Size:         89     623      95

  Fragments arranged by size: 

                 623      95      89
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     628     662     807 
   Size:        628      34     145

  Fragments arranged by size: 

                 628     145      34
 
BfrBI ATG'CAT

Cuts at:      0     352     807 
   Size:        352     455
 
Bme1580I G_kGCm'C

Cuts at:      0     712     807 
   Size:        712      95
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     706     807 
   Size:        706     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     240     350     807 
   Size:        119     121     110     457

  Fragments arranged by size: 

                 457     121     119     110
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     326     538     807 
   Size:        326     212     269

  Fragments arranged by size: 

                 326     269     212
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     635     807 
   Size:        635     172
 
BseYI C'CCAG_C

Cuts at:      0     323     807 
   Size:        323     484
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     712     807 
   Size:         89     623      95

  Fragments arranged by size: 

                 623      95      89
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     178     807 
   Size:        178     629
 
BsrFI r'CCGG_y

Cuts at:      0      47     807 
   Size:         47     760
 
BstYI r'GATC_y

Cuts at:      0     697     807 
   Size:        697     110
 
DraI TTT'AAA

Cuts at:      0     309     807 
   Size:        309     498
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     430     807 
   Size:        430     377
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     758     807 
   Size:        758      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     240     350     807 
   Size:        119     121     110     457

  Fragments arranged by size: 

                 457     121     119     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     708     807 
   Size:        708      99
 
EcoRV GAT'ATC

Cuts at:      0     795     807 
   Size:        795      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     304     807 
   Size:        304     503
 
HaeII r_GCGC'y

Cuts at:      0      54      82     807 
   Size:         54      28     725

  Fragments arranged by size: 

                 725      54      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     610     642     807 
   Size:        610      32     165

  Fragments arranged by size: 

                 610     165      32
 
HincII GTy'rAC

Cuts at:      0     206     807 
   Size:        206     601
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     686     807 
   Size:        686     121
 
MslI CAynn'nnrTG

Cuts at:      0     250     550     752     807 
   Size:        250     300     202      55

  Fragments arranged by size: 

                 300     250     202      55
 
MspA1I CmG'CkG

Cuts at:      0      97     323     807 
   Size:         97     226     484

  Fragments arranged by size: 

                 484     226      97
 
NaeI GCC'GGC

Cuts at:      0      49     807 
   Size:         49     758
 
NgoMIV G'CCGG_C

Cuts at:      0      47     807 
   Size:         47     760
 
NsiI A_TGCA'T

Cuts at:      0     354     807 
   Size:        354     453
 
PfoI T'CCnGG_A

Cuts at:      0     217     807 
   Size:        217     590
 
PshAI GACnn'nnGTC

Cuts at:      0     211     807 
   Size:        211     596
 
PspOMI G'GGCC_C

Cuts at:      0     708     807 
   Size:        708      99
 
PstI C_TGCA'G

Cuts at:      0     610     807 
   Size:        610     197
 
PvuII CAG'CTG

Cuts at:      0      97     323     807 
   Size:         97     226     484

  Fragments arranged by size: 

                 484     226      97
 
SfcI C'TryA_G

Cuts at:      0     570     606     807 
   Size:        570      36     201

  Fragments arranged by size: 

                 570     201      36
 
SpeI A'CTAG_T

Cuts at:      0     187     807 
   Size:        187     620
 
StyI C'CwwG_G

Cuts at:      0     110     273     288     528     807 
   Size:        110     163      15     240     279

  Fragments arranged by size: 

                 279     240     163     110      15
 
TatI w'GTAC_w

Cuts at:      0     610     807 
   Size:        610     197
 

 Enzymes that do cut:

     AccI     AleI    AlwNI     ApaI     ApoI    AvrII     BanI    BanII
     BcgI    BfrBI Bme1580I     BmrI     BpmI     BsaI    BseRI    BseYI
 Bsp1286I    BspMI    BsrFI    BstYI     DraI     DrdI     EciI  Eco57MI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII     MmeI     MslI
   MspA1I     NaeI   NgoMIV     NsiI     PfoI    PshAI   PspOMI     PstI
    PvuII     SfcI     SpeI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     EaeI     EagI
     EarI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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