Restriction map for pF1KB5535
 (Circular) (Six-base) MAPSORT of: pF1KB5535.seq  Check: 5784  from: 1  to: 4502



pF1KB5535  4502 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    195     195 
   Size:       4502
 
AccI GT'mk_AC

Cuts at:    924    1540     924 
   Size:        616    3886
 
Acc65I G'GTAC_C

Cuts at:   1518    1518 
   Size:       4502
 
AclI AA'CG_TT

Cuts at:   4342    4342 
   Size:       4502
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    295    2327    2759    3506    3774     295 
   Size:       2032     432     747     268    1023

  Fragments arranged by size: 

                2032    1023     747     432     268
 
AflII C'TTAA_G

Cuts at:    574     574 
   Size:       4502
 
AflIII A'CryG_T

Cuts at:   1854    2958    3935    1854 
   Size:       1104     977    2421

  Fragments arranged by size: 

                2421    1104     977
 
AgeI A'CCGG_T

Cuts at:   2890    2890 
   Size:       4502
 
AlwNI CAG_nnn'CTG

Cuts at:    318    1087    1360    1807    3374     318 
   Size:        769     273     447    1567    1446

  Fragments arranged by size: 

                1567    1446     769     447     273
 
ApaI G_GGCC'C

Cuts at:    122    1214     122 
   Size:       1092    3410
 
ApaLI G'TGCA_C

Cuts at:   3272    3272 
   Size:       4502
 
ApoI r'AATT_y

Cuts at:    579    1506    3855    4111     579 
   Size:        927    2349     256     970

  Fragments arranged by size: 

                2349     970     927     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4502
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4502
 
AvaI C'yCGr_G

Cuts at:   1522    1522 
   Size:       4502
 
BamHI G'GATC_C

Cuts at:   1427    1527    1427 
   Size:        100    4402
 
BanI G'GyrC_C

Cuts at:    166     217    1396    1518    1932    2180    2215     166 
   Size:         51    1179     122     414     248      35    2453

  Fragments arranged by size: 

                2453    1179     414     248     122      51      35
 
BanII G_rGCy'C

Cuts at:    122     241    1214    1516    2546     122 
   Size:        119     973     302    1030    2078

  Fragments arranged by size: 

                2078    1030     973     302     119
 
BbeI G_GCGC'C

Cuts at:    170    1936    2184     170 
   Size:       1766     248    2488

  Fragments arranged by size: 

                2488    1766     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    219    1118     219 
   Size:        899    3603
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    169     203     169 
   Size:         34    4468
 
BciVI GTATCCnnnnn_n'

Cuts at:    315    2400    3161    4502     315 
   Size:       2085     761    1341     315

  Fragments arranged by size: 

                2085    1341     761     315
 
BclI T'GATC_A

Cuts at:    618    2022     618 
   Size:       1404    3098
 
BglII A'GATC_T

Cuts at:   2017    4054    2017 
   Size:       2037    2465
 
BlpI GC'TnA_GC

Cuts at:   1614    1614 
   Size:       4502
 
Bme1580I G_kGCm'C

Cuts at:    122     220    1214    1399    2127    2220    3276     122 
   Size:         98     994     185     728      93    1056    1348

  Fragments arranged by size: 

                1348    1056     994     728     185      98      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    485     516    1714    1795    2130    4274     485 
   Size:         31    1198      81     335    2144     713

  Fragments arranged by size: 

                2144    1198     713     335      81      31
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2869    2869 
   Size:       4502
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    225     837     225 
   Size:        612    3890
 
Bpu10I CC'TnA_GC

Cuts at:    955     955 
   Size:       4502
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    977    1213    1678    2557    3049    3347     977 
   Size:        236     465     879     492     298    2132

  Fragments arranged by size: 

                2132     879     492     465     298     236
 
BsaI GGTCTCn'nnnn_

Cuts at:   1283    4206    1283 
   Size:       2923    1579
 
BsaAI yAC'GTr

Cuts at:   1855    1855 
   Size:       4502
 
BsaBI GATnn'nnATC

Cuts at:      6    2016    4053       6 
   Size:       2010    2037     455

  Fragments arranged by size: 

                2037    2010     455
 
BsaHI Gr'CG_yC

Cuts at:    167    1933    2181    2883    4379     167 
   Size:       1766     248     702    1496     290

  Fragments arranged by size: 

                1766    1496     702     290     248
 
BsaWI w'CCGG_w

Cuts at:    520    1424    1691    2212    2890    3164    3311    3722     520 
   Size:        904     267     521     678     274     147     411    1300

  Fragments arranged by size: 

                1300     904     678     521     411     274     267     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    708     738     958     988    1077    1107     708 
   Size:         30     220      30      89      30    4103

  Fragments arranged by size: 

                4103     220      89      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1122    1122 
   Size:       4502
 
BseYI C'CCAG_C

Cuts at:    115     213     251    1391    1403    1927    2685    3262    3758 
   Size:         98      38    1140      12     524     758     577     496

Cuts at:   3758     115 
   Size:        859

  Fragments arranged by size: 

                1140     859     758     577     524     496      98      38
                  12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    180     743     180 
   Size:        563    3939
 
BsiEI CG_ry'CG

Cuts at:    104    2044    2090    3298     104 
   Size:       1940      46    1208    1308

  Fragments arranged by size: 

                1940    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1516    2294    2484    3276    1516 
   Size:        778     190     792    2742

  Fragments arranged by size: 

                2742     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    122     220     241    1214    1399    1516    2127    2220    2294 
   Size:         98      21     973     185     117     611      93      74

Cuts at:   2294    2484    2546    3276     122 
   Size:        190      62     730    1348

  Fragments arranged by size: 

                1348     973     730     611     190     185     117      98
                  93      74      62      21
 
BspEI T'CCGG_A

Cuts at:   1424    3722    1424 
   Size:       2298    2204
 
BspHI T'CATG_A

Cuts at:   3678    3678 
   Size:       4502
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    195     218     765    1552    1766    2068    2449     195 
   Size:         23     547     787     214     302     381    2248

  Fragments arranged by size: 

                2248     787     547     381     302     214      23
 
BsrBI CCG'CTC

Cuts at:     50    2794    2848    4332    4497      50 
   Size:       2744      54    1484     165      55

  Fragments arranged by size: 

                2744    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1179    2414    1179 
   Size:       1235    3267
 
BsrFI r'CCGG_y

Cuts at:   1399    2500    2681    2890    1399 
   Size:       1101     181     209    3011

  Fragments arranged by size: 

                3011    1101     209     181
 
BssSI C'ACGA_G

Cuts at:   2773    3131    2773 
   Size:        358    4144
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1879    1879 
   Size:       4502
 
BstBI TT'CG_AA

Cuts at:    855    2863     855 
   Size:       2008    2494
 
BstEII G'GTnAC_C

Cuts at:    447     447 
   Size:       4502
 
BstXI CCAn_nnnn'nTGG

Cuts at:    809    1048    1279     809 
   Size:        239     231    4032

  Fragments arranged by size: 

                4032     239     231
 
BstYI r'GATC_y

Cuts at:      1    1265    1427    1527    1994    2017    2352    2598    3610 
   Size:       1264     162     100     467      23     335     246    1012

Cuts at:   3610    3696    3708    4054       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1264    1012     467     449     346     335     246     162
                 100      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    925     925 
   Size:       4502
 
Bsu36I CC'TnA_GG

Cuts at:    962     962 
   Size:       4502
 
BtgI C'CryG_G

Cuts at:    107    1214     107 
   Size:       1107    3395
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    272    2452    2633    2796     272 
   Size:       2180     181     163    1978

  Fragments arranged by size: 

                2180    1978     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1475    1745    1475 
   Size:        270    4232
 
DraI TTT'AAA

Cuts at:   1501    1501 
   Size:       4502
 
DrdI GACnn_nn'nnGTC

Cuts at:   2208    3066    2208 
   Size:        858    3644
 
EaeI y'GGCC_r

Cuts at:    351     726    2087    2261    2652    2679    4024     351 
   Size:        375    1361     174     391      27    1345     829

  Fragments arranged by size: 

                1361    1345     829     391     375     174      27
 
EagI C'GGCC_G

Cuts at:   2087    2087 
   Size:       4502
 
EarI CTCTTCn'nnn_

Cuts at:    428    2525    2735     428 
   Size:       2097     210    2195

  Fragments arranged by size: 

                2195    2097     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2710    3020    3166    4312    2710 
   Size:        310     146    1146    2900

  Fragments arranged by size: 

                2900    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1514    1514 
   Size:       4502
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    225     295     837    2327    2759    3506    3774     225 
   Size:         70     542    1490     432     747     268     953

  Fragments arranged by size: 

                1490     953     747     542     432     268      70
 
EcoO109I rG'GnC_Cy

Cuts at:    119    1641     119 
   Size:       1522    2980
 
EcoRI G'AATT_C

Cuts at:    579    1506     579 
   Size:        927    3575
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1310    2521    1310 
   Size:       1211    3291
 
FspI TGC'GCA

Cuts at:   2283    2283 
   Size:       4502
 
HaeII r_GCGC'y

Cuts at:    170    1936    2184    3206    4185     170 
   Size:       1766     248    1022     979     487

  Fragments arranged by size: 

                1766    1022     979     487     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    958     990    1342    1374    2344    2374    2376    2406     958 
   Size:         32     352      32     970      30       2      30    3054

  Fragments arranged by size: 

                3054     970     352      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    264     561    1541     264 
   Size:        297     980    3225

  Fragments arranged by size: 

                3225     980     297
 
HindIII A'AGCT_T

Cuts at:   1064    1189    1064 
   Size:        125    4377
 
KasI G'GCGC_C

Cuts at:    166    1932    2180     166 
   Size:       1766     248    2488

  Fragments arranged by size: 

                2488    1766     248
 
KpnI G_GTAC'C

Cuts at:   1522    1522 
   Size:       4502
 
MfeI C'AATT_G

Cuts at:   1454    1454 
   Size:       4502
 
MluI A'CGCG_T

Cuts at:   3935    3935 
   Size:       4502
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3173    3357    3173 
   Size:        184    4318
 
MscI TGG'CCA

Cuts at:    353     728    2263     353 
   Size:        375    1535    2592

  Fragments arranged by size: 

                2592    1535     375
 
MslI CAynn'nnrTG

Cuts at:   1468    2491    2618    1468 
   Size:       1023     127    3352

  Fragments arranged by size: 

                3352    1023     127
 
MspA1I CmG'CkG

Cuts at:    245     878    1391    1613    1927    2287    3300    3545     245 
   Size:        633     513     222     314     360    1013     245    1202

  Fragments arranged by size: 

                1202    1013     633     513     360     314     245     222
            
 
NaeI GCC'GGC

Cuts at:   2683    2683 
   Size:       4502
 
NarI GG'CG_CC

Cuts at:    167    1933    2181     167 
   Size:       1766     248    2488

  Fragments arranged by size: 

                2488    1766     248
 
NcoI C'CATG_G

Cuts at:    107    1214     107 
   Size:       1107    3395
 
NdeI CA'TA_TG

Cuts at:    330     330 
   Size:       4502
 
NgoMIV G'CCGG_C

Cuts at:   2681    2681 
   Size:       4502
 
NspI r_CATG'y

Cuts at:   1555    2055    2962    1555 
   Size:        500     907    3095

  Fragments arranged by size: 

                3095     907     500
 
PasI CC'CwG_GG

Cuts at:    699     699 
   Size:       4502
 
PciI A'CATG_T

Cuts at:   2958    2958 
   Size:       4502
 
PmeI GTTT'AAAC

Cuts at:   1501    1501 
   Size:       4502
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3641    3673    3641 
   Size:         32    4470
 
PspOMI G'GGCC_C

Cuts at:    118    1210     118 
   Size:       1092    3410
 
PstI C_TGCA'G

Cuts at:   1549    2234    1549 
   Size:        685    3817
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4502
 
PvuII CAG'CTG

Cuts at:    878    1391    1927    2287     878 
   Size:        513     536     360    3093

  Fragments arranged by size: 

                3093     536     513     360
 
RsrII CG'GwC_CG

Cuts at:   2697    2697 
   Size:       4502
 
SacI G_AGCT'C

Cuts at:   1516    1516 
   Size:       4502
 
SalI G'TCGA_C

Cuts at:   1539    1539 
   Size:       4502
 
SapI GCTCTTCn'nnn_

Cuts at:   2525    2735    2525 
   Size:        210    4292
 
SbfI CC_TGCA'GG

Cuts at:   1549    1549 
   Size:       4502
 
ScaI AGT'ACT

Cuts at:   4019    4019 
   Size:       4502
 
SfcI C'TryA_G

Cuts at:     33    1545    2230    3223    3414      33 
   Size:       1512     685     993     191    1121

  Fragments arranged by size: 

                1512    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:    168    1934    2182     168 
   Size:       1766     248    2488

  Fragments arranged by size: 

                2488    1766     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4502
 
SmaI CCC'GGG

Cuts at:   1524    1524 
   Size:       4502
 
SmlI C'TyrA_G

Cuts at:    574     992    1192    1657    2572    3064    3326     574 
   Size:        418     200     465     915     492     262    1750

  Fragments arranged by size: 

                1750     915     492     465     418     262     200
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4502
 
SphI G_CATG'C

Cuts at:   1555    2055    1555 
   Size:        500    4002
 
StyI C'CwwG_G

Cuts at:    107     339     375     745    1214    1636    1990     107 
   Size:        232      36     370     469     422     354    2619

  Fragments arranged by size: 

                2619     469     422     370     354     232      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    100     467     471    2085    2218    2677    2886     100 
   Size:        367       4    1614     133     459     209    1716

  Fragments arranged by size: 

                1716    1614     459     367     209     133       4
 
TatI w'GTAC_w

Cuts at:   4017    4017 
   Size:       4502
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    515     547     708     740    1077    1109    1470    1502    4019 
   Size:         32     161      32     337      32     361      32    2517

Cuts at:   4019    4051     515 
   Size:         32     966

  Fragments arranged by size: 

                2517     966     361     337     161      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2299    2299 
   Size:       4502
 
XbaI T'CTAG_A

Cuts at:   1533    1533 
   Size:       4502
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    868    1997     868 
   Size:       1129    3373
 
XmaI C'CCGG_G

Cuts at:   1522    1522 
   Size:       4502
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NspI     PasI     PciI     PmeI     PpiI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AleI     AlfI     AloI     AscI    AvrII
     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI     HpaI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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