Restriction map for pF1KB5535
 (Linear) (Six-base) MAPSORT of: KIBB5535.seq  Check: 9634  from: 1  to: 1389



KIBB5535.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      87    1389 
   Size:         87    1302
 
AccI GT'mk_AC

Cuts at:      0     816    1389 
   Size:        816     573
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187    1389 
   Size:        187    1202
 
AflII C'TTAA_G

Cuts at:      0     466    1389 
   Size:        466     923
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210     979    1252    1389 
   Size:        210     769     273     137

  Fragments arranged by size: 

                 769     273     210     137
 
ApaI G_GGCC'C

Cuts at:      0      14    1106    1389 
   Size:         14    1092     283

  Fragments arranged by size: 

                1092     283      14
 
ApoI r'AATT_y

Cuts at:      0     471    1389 
   Size:        471     918
 
BamHI G'GATC_C

Cuts at:      0    1319    1389 
   Size:       1319      70
 
BanI G'GyrC_C

Cuts at:      0      58     109    1288    1389 
   Size:         58      51    1179     101

  Fragments arranged by size: 

                1179     101      58      51
 
BanII G_rGCy'C

Cuts at:      0      14     133    1106    1389 
   Size:         14     119     973     283

  Fragments arranged by size: 

                 973     283     119      14
 
BbeI G_GCGC'C

Cuts at:      0      62    1389 
   Size:         62    1327
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     111    1010    1389 
   Size:        111     899     379

  Fragments arranged by size: 

                 899     379     111
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      61      95    1389 
   Size:         61      34    1294

  Fragments arranged by size: 

                1294      61      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     207    1389 
   Size:        207    1182
 
BclI T'GATC_A

Cuts at:      0     510    1389 
   Size:        510     879
 
Bme1580I G_kGCm'C

Cuts at:      0      14     112    1106    1291    1389 
   Size:         14      98     994     185      98

  Fragments arranged by size: 

                 994     185      98      98      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     377     408    1389 
   Size:        377      31     981

  Fragments arranged by size: 

                 981     377      31
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     729    1389 
   Size:        117     612     660

  Fragments arranged by size: 

                 660     612     117
 
Bpu10I CC'TnA_GC

Cuts at:      0     847    1389 
   Size:        847     542
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     869    1105    1389 
   Size:        869     236     284

  Fragments arranged by size: 

                 869     284     236
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1175    1389 
   Size:       1175     214
 
BsaHI Gr'CG_yC

Cuts at:      0      59    1389 
   Size:         59    1330
 
BsaWI w'CCGG_w

Cuts at:      0     412    1316    1389 
   Size:        412     904      73

  Fragments arranged by size: 

                 904     412      73
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     600     630     850     880     969     999    1389 
   Size:        600      30     220      30      89      30     390

  Fragments arranged by size: 

                 600     390     220      89      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1014    1389 
   Size:       1014     375
 
BseYI C'CCAG_C

Cuts at:      0       7     105     143    1283    1295    1389 
   Size:          7      98      38    1140      12      94

  Fragments arranged by size: 

                1140      98      94      38      12       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     635    1389 
   Size:         72     563     754

  Fragments arranged by size: 

                 754     563      72
 
Bsp1286I G_dGCh'C

Cuts at:      0      14     112     133    1106    1291    1389 
   Size:         14      98      21     973     185      98

  Fragments arranged by size: 

                 973     185      98      98      21      14
 
BspEI T'CCGG_A

Cuts at:      0    1316    1389 
   Size:       1316      73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      87     110     657    1389 
   Size:         87      23     547     732

  Fragments arranged by size: 

                 732     547      87      23
 
BsrDI GCAATG_nn'

Cuts at:      0    1071    1389 
   Size:       1071     318
 
BsrFI r'CCGG_y

Cuts at:      0    1291    1389 
   Size:       1291      98
 
BstBI TT'CG_AA

Cuts at:      0     747    1389 
   Size:        747     642
 
BstEII G'GTnAC_C

Cuts at:      0     339    1389 
   Size:        339    1050
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     701     940    1171    1389 
   Size:        701     239     231     218

  Fragments arranged by size: 

                 701     239     231     218
 
BstYI r'GATC_y

Cuts at:      0    1157    1319    1389 
   Size:       1157     162      70

  Fragments arranged by size: 

                1157     162      70
 
BstZ17I GTA'TAC

Cuts at:      0     817    1389 
   Size:        817     572
 
Bsu36I CC'TnA_GG

Cuts at:      0     854    1389 
   Size:        854     535
 
BtgI C'CryG_G

Cuts at:      0    1106    1389 
   Size:       1106     283
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     164    1389 
   Size:        164    1225
 
BtsI GCAGTG_nn'

Cuts at:      0    1367    1389 
   Size:       1367      22
 
EaeI y'GGCC_r

Cuts at:      0     243     618    1389 
   Size:        243     375     771

  Fragments arranged by size: 

                 771     375     243
 
EarI CTCTTCn'nnn_

Cuts at:      0     320    1389 
   Size:        320    1069
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     187     729    1389 
   Size:        117      70     542     660

  Fragments arranged by size: 

                 660     542     117      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0      11    1389 
   Size:         11    1378
 
EcoRI G'AATT_C

Cuts at:      0     471    1389 
   Size:        471     918
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1202    1389 
   Size:       1202     187
 
HaeII r_GCGC'y

Cuts at:      0      62    1389 
   Size:         62    1327
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     850     882    1234    1266    1389 
   Size:        850      32     352      32     123

  Fragments arranged by size: 

                 850     352     123      32      32
 
HincII GTy'rAC

Cuts at:      0     156     453    1389 
   Size:        156     297     936

  Fragments arranged by size: 

                 936     297     156
 
HindIII A'AGCT_T

Cuts at:      0     956    1081    1389 
   Size:        956     125     308

  Fragments arranged by size: 

                 956     308     125
 
KasI G'GCGC_C

Cuts at:      0      58    1389 
   Size:         58    1331
 
MfeI C'AATT_G

Cuts at:      0    1346    1389 
   Size:       1346      43
 
MscI TGG'CCA

Cuts at:      0     245     620    1389 
   Size:        245     375     769

  Fragments arranged by size: 

                 769     375     245
 
MslI CAynn'nnrTG

Cuts at:      0    1360    1389 
   Size:       1360      29
 
MspA1I CmG'CkG

Cuts at:      0     137     770    1283    1389 
   Size:        137     633     513     106

  Fragments arranged by size: 

                 633     513     137     106
 
NarI GG'CG_CC

Cuts at:      0      59    1389 
   Size:         59    1330
 
NcoI C'CATG_G

Cuts at:      0    1106    1389 
   Size:       1106     283
 
NdeI CA'TA_TG

Cuts at:      0     222    1389 
   Size:        222    1167
 
PasI CC'CwG_GG

Cuts at:      0     591    1389 
   Size:        591     798
 
PspOMI G'GGCC_C

Cuts at:      0      10    1102    1389 
   Size:         10    1092     287

  Fragments arranged by size: 

                1092     287      10
 
PvuII CAG'CTG

Cuts at:      0     770    1283    1389 
   Size:        770     513     106

  Fragments arranged by size: 

                 770     513     106
 
SfoI GGC'GCC

Cuts at:      0      60    1389 
   Size:         60    1329
 
SmlI C'TyrA_G

Cuts at:      0     466     884    1084    1389 
   Size:        466     418     200     305

  Fragments arranged by size: 

                 466     418     305     200
 
StyI C'CwwG_G

Cuts at:      0     231     267     637    1106    1389 
   Size:        231      36     370     469     283

  Fragments arranged by size: 

                 469     370     283     231      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     359     363    1389 
   Size:        359       4    1026

  Fragments arranged by size: 

                1026     359       4
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     407     439     600     632     969    1001    1362    1389 
   Size:        407      32     161      32     337      32     361      27

  Fragments arranged by size: 

                 407     361     337     161      32      32      32      27
            
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     760    1389 
   Size:        760     629
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII    AlwNI     ApaI     ApoI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I   HincII  HindIII     KasI     MfeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     PasI   PspOMI    PvuII     SfoI     SmlI
     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BbvCI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI    BsaAI    BsaBI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspHI    BsrBI    BsrGI   BssHII    BssSI   BstAPI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI     HpaI     KpnI     MluI     MmeI     NaeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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