Restriction map for pF1KB5503
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BlnI        BsaBI       BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      EcoO65I     
EcoRI       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        StyI        SwaI        Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          727  GTCTAC               GT^[AC][GT]AC
AcyI         1437  GGCGTC               G[GA]^CG[CT]C
AhdI          709  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1339  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          400  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       640  AGCGCT               AGC^GCT
ApaI          487  GGGCCC               GGGCC^C
AspEI         709  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         961  GTGCTC               G[AT]GC[AT]^C
AvaI           41  CCCGGG               C^[CT]CG[GA]G
AvaII         336  GGACC                G^G[AT]CC
BamHI         934  GGATCC               G^GATCC
BglI            7  GCCGCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1616  AGATCT               A^GATCT
BlpI         1198  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1198  GCTCAGC              GC^T[ACGT]AGC
BsaHI        1437  GGCGTC               G[GA]^CG[CT]C
BsiEI           5  CGGCCG               CG[GA][CT]^CG
BsiHKAI       961  GTGCTC               G[AT]GC[AT]^C
BsoBI          41  CCCGGG               C^[CT]CG[GA]G
BsrBI          97  CCGCTC               CCGCTC(-3/-3)
BsrDI         832  GCAATG               GCAATG(2/0)
BsrFI        1271  ACCGGC               [GA]^CCGG[CT]
BssHII         51  GCGCGC               G^CGCGC
Bsu36I        400  CCTCAGG              CC^T[ACGT]AGG
CelII        1198  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1271  ACCGGC               [GA]^CCGG[CT]
DrdI         1629  GACCCGCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          113  CCACGG               C^C[GA][CT]GG
EagI            5  CGGCCG               C^GGCCG
Eam1105I      709  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI           5  CGGCCG               C^GGCCG
Eco47III      640  AGCGCT               AGC^GCT
Eco52I          5  CGGCCG               C^GGCCG
Eco81I        400  CCTCAGG              CC^T[ACGT]AGG
EcoRV         438  GATATC               GAT^ATC
HgaI         1438  GCGTC                GACGC(5/10)
Hin1I        1437  GGCGTC               G[GA]^CG[CT]C
HincII       1090  GTTGAC               GT[CT]^[GA]AC
HindII       1090  GTTGAC               GT[CT]^[GA]AC
NotI            4  GCGGCCGC             GC^GGCCGC
NspI          887  ACATGC               [GA]CATG^[CT]
PvuII        1207  CAGCTG               CAG^CTG
SfcI         1403  CTGTAG               C^T[GA][CT]AG
SmaI           41  CCCGGG               CCC^GGG
SmlI          661  CTCAAG               C^T[CT][GA]AG
TaqI         1133  TCGA                 T^CGA
TthHB8I      1133  TCGA                 T^CGA
XmaCI          41  CCCGGG               C^CCGGG
XmaI           41  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII          52  CGCG                 CG^CG
              209  CGCG                
AlwI          935  GATCC                GGATC(4/5)
              934  GGATC               
BbsI          108  GAAGAC               GAAGAC(2/6)
             1656  GAAGAC              
BfaI           31  CTAG                 C^TAG
             1267  CTAG                
BpuAI         108  GAAGAC               GAAGAC(2/6)
             1656  GAAGAC              
BsaAI         433  TACGTG               [CT]AC^GT[GA]
              979  TACGTG              
BsaI          118  GAGACC               GGTCTC(1/5)
             1627  GAGACC              
BsmAI         118  GAGAC                GTCTC(1/5)
             1627  GAGAC               
BsmI          162  GAATGC               GAATGC(1/-1)
              777  GAATGC              
Bsp1407I     1007  TGTACA               T^GTACA
             1394  TGTACA              
BsrGI        1007  TGTACA               T^GTACA
             1394  TGTACA              
BstUI          52  CGCG                 CG^CG
              209  CGCG                
BstXI          80  CCATCGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              147  CCAGATCAGTGG        
BstYI         934  GGATCC               [GA]^GATC[CT]
             1616  AGATCT              
DraII         350  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1233  AGGGCCT             
EcoNI         320  CCTATCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              621  CCTTCCAGAGG         
EcoO109I      350  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1233  AGGGCCT             
MaeI           31  CTAG                 C^TAG
             1267  CTAG                
MflI          934  GGATCC               [GA]^GATC[CT]
             1616  AGATCT              
MseI          536  TTAA                 T^TAA
             1370  TTAA                
MslI          195  CACTGTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              252  CACCGTTGTG          
MvnI           52  CGCG                 CG^CG
              209  CGCG                
SfaNI        1384  GCATC                GCATC(5/9)
             1453  GCATC               
SspBI        1007  TGTACA               T^GTACA
             1394  TGTACA              
StuI          317  AGGCCT               AGG^CCT
              530  AGGCCT              
Tru9I         536  TTAA                 T^TAA
             1370  TTAA                
XcmI          625  CCAGAGGACAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              872  CCAGCTACTATTTGG     
XhoII         934  GGATCC               [GA]^GATC[CT]
             1616  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        BalI        BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       TfiI        TseI        Tsp45I      TspRI       
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