Restriction map for pF1KB5503
 (Circular) (Six-base) MAPSORT of: pF1KB5503.seq  Check: 1435  from: 1  to: 4778



pF1KB5503  4778 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:    836    1816     836 
   Size:        980    3798
 
Acc65I G'GTAC_C

Cuts at:   1794    1794 
   Size:       4778
 
AclI AA'CG_TT

Cuts at:   4618    4618 
   Size:       4778
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    262     783    2603    3035    3782    4050     262 
   Size:        521    1820     432     747     268     990

  Fragments arranged by size: 

                1820     990     747     521     432     268
 
AfeI AGC'GCT

Cuts at:    750     750 
   Size:       4778
 
AflIII A'CryG_T

Cuts at:   2130    3234    4211    2130 
   Size:       1104     977    2697

  Fragments arranged by size: 

                2697    1104     977
 
AgeI A'CCGG_T

Cuts at:   3166    3166 
   Size:       4778
 
AhdI GACnn_n'nnGTC

Cuts at:    822     822 
   Size:       4778
 
AleI CACnn'nnGTG

Cuts at:    307     364     307 
   Size:         57    4721
 
AlwNI CAG_nnn'CTG

Cuts at:   1452    2083    3650    1452 
   Size:        631    1567    2580

  Fragments arranged by size: 

                2580    1567     631
 
ApaI G_GGCC'C

Cuts at:    599     599 
   Size:       4778
 
ApaLI G'TGCA_C

Cuts at:   3548    3548 
   Size:       4778
 
ApoI r'AATT_y

Cuts at:   1782    4131    4387    1782 
   Size:       2349     256    2173

  Fragments arranged by size: 

                2349    2173     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4778
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4778
 
AvaI C'yCGr_G

Cuts at:    149    1798     149 
   Size:       1649    3129
 
BamHI G'GATC_C

Cuts at:   1042    1803    1042 
   Size:        761    4017
 
BanI G'GyrC_C

Cuts at:    294     895    1553    1794    2208    2456    2491     294 
   Size:        601     658     241     414     248      35    2581

  Fragments arranged by size: 

                2581     658     601     414     248     241      35
 
BanII G_rGCy'C

Cuts at:    529     599    1395    1792    2822     529 
   Size:         70     796     397    1030    2485

  Fragments arranged by size: 

                2485    1030     796     397      70
 
BbeI G_GCGC'C

Cuts at:   2212    2460    2212 
   Size:        248    4530
 
BbsI GAAGACnn'nnnn_

Cuts at:    223    1771     223 
   Size:       1548    3230
 
BciVI GTATCCnnnnn_n'

Cuts at:   2676    3437    4778    2676 
   Size:        761    1341    2676

  Fragments arranged by size: 

                2676    1341     761
 
BclI T'GATC_A

Cuts at:   2298    2298 
   Size:       4778
 
BglI GCCn_nnn'nGGC

Cuts at:    112     121     112 
   Size:          9    4769
 
BglII A'GATC_T

Cuts at:   1724    2293    4330    1724 
   Size:        569    2037    2172

  Fragments arranged by size: 

                2172    2037     569
 
BlpI GC'TnA_GC

Cuts at:   1307    1890    1307 
   Size:        583    4195
 
Bme1580I G_kGCm'C

Cuts at:    332     599    1556    1703    2403    2496    3552     332 
   Size:        267     957     147     700      93    1056    1558

  Fragments arranged by size: 

                1558    1056     957     700     267     147      93
 
BmgBI CAC'GTC

Cuts at:   1488    1488 
   Size:       4778
 
BmrI ACTGGGnnnn_n'

Cuts at:    849    1018    1990    2071    2406    4550     849 
   Size:        169     972      81     335    2144    1077

  Fragments arranged by size: 

                2144    1077     972     335     169      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    782    3145     782 
   Size:       2363    2415
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    963    1124    1753    1770     963 
   Size:        161     629      17    3971

  Fragments arranged by size: 

                3971     629     161      17
 
Bpu10I CC'TnA_GC

Cuts at:   1094    1094 
   Size:       4778
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    754    1954    2833    3325    3623     754 
   Size:       1200     879     492     298    1909

  Fragments arranged by size: 

                1909    1200     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    220    1729    4482     220 
   Size:       1509    2753     516

  Fragments arranged by size: 

                2753    1509     516
 
BsaAI yAC'GTr

Cuts at:    543    1089    2131     543 
   Size:        546    1042    3190

  Fragments arranged by size: 

                3190    1042     546
 
BsaBI GATnn'nnATC

Cuts at:      6    2292    4329       6 
   Size:       2286    2037     455

  Fragments arranged by size: 

                2286    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1546    2209    2457    3159    4655    1546 
   Size:        663     248     702    1496    1669

  Fragments arranged by size: 

                1669    1496     702     663     248
 
BsaWI w'CCGG_w

Cuts at:   1967    2488    3166    3440    3587    3998    1967 
   Size:        521     678     274     147     411    2747

  Fragments arranged by size: 

                2747     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    448     478     448 
   Size:         30    4748
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    466     469     549    1618     466 
   Size:          3      80    1069    3626

  Fragments arranged by size: 

                3626    1069      80       3
 
BseYI C'CCAG_C

Cuts at:    195    1038    1172    1313    2203    2961    3538    4034     195 
   Size:        843     134     141     890     758     577     496     939

  Fragments arranged by size: 

                 939     890     843     758     577     496     141     134
            
 
BsiEI CG_ry'CG

Cuts at:    104     116    2320    2366    3574     104 
   Size:         12    2204      46    1208    1308

  Fragments arranged by size: 

                2204    1308    1208      46      12
 
BsiHKAI G_wGCw'C

Cuts at:   1073    1792    2570    2760    3552    1073 
   Size:        719     778     190     792    2299

  Fragments arranged by size: 

                2299     792     778     719     190
 
BsmI GAATG_Cn'

Cuts at:    276     891     276 
   Size:        615    4163
 
Bsp1286I G_dGCh'C

Cuts at:    332     529     599    1073    1395    1556    1703    1792    2403 
   Size:        197      70     474     322     161     147      89     611

Cuts at:   2403    2496    2570    2760    2822    3552     332 
   Size:         93      74     190      62     730    1558

  Fragments arranged by size: 

                1558     730     611     474     322     197     190     161
                 147      93      89      74      70      62
 
BspEI T'CCGG_A

Cuts at:   3998    3998 
   Size:       4778
 
BspHI T'CATG_A

Cuts at:   3954    3954 
   Size:       4778
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1828    2042    2344    2725    1828 
   Size:        214     302     381    3881

  Fragments arranged by size: 

                3881     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     207    3070    3124    4608    4773      50 
   Size:        157    2863      54    1484     165      55

  Fragments arranged by size: 

                2863    1484     165     157      55      54
 
BsrDI GCAATG_nn'

Cuts at:    947    2690     947 
   Size:       1743    3035
 
BsrFI r'CCGG_y

Cuts at:   1379    2776    2957    3166    1379 
   Size:       1397     181     209    2991

  Fragments arranged by size: 

                2991    1397     209     181
 
BsrGI T'GTAC_A

Cuts at:   1115    1502    1115 
   Size:        387    4391
 
BssHII G'CGCG_C

Cuts at:    159     159 
   Size:       4778
 
BssSI C'ACGA_G

Cuts at:   3049    3407    3049 
   Size:        358    4420
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2155    2155 
   Size:       4778
 
BstBI TT'CG_AA

Cuts at:   3139    3139 
   Size:       4778
 
BstXI CCAn_nnnn'nTGG

Cuts at:    195     262     195 
   Size:         67    4711
 
BstYI r'GATC_y

Cuts at:      1    1042    1724    1803    2270    2293    2628    2874    3886 
   Size:       1041     682      79     467      23     335     246    1012

Cuts at:   3886    3972    3984    4330       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1041    1012     682     467     449     346     335     246
                  86      79      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    509     509 
   Size:       4778
 
BtgI C'CryG_G

Cuts at:    107     221     107 
   Size:        114    4664
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2728    2909    3072    2728 
   Size:        181     163    4434

  Fragments arranged by size: 

                4434     181     163
 
BtsI GCAGTG_nn'

Cuts at:    480     617    2021     480 
   Size:        137    1404    3237

  Fragments arranged by size: 

                3237    1404     137
 
DraI TTT'AAA

Cuts at:   1777    1777 
   Size:       4778
 
DrdI GACnn_nn'nnGTC

Cuts at:   1743    2484    3342    1743 
   Size:        741     858    3179

  Fragments arranged by size: 

                3179     858     741
 
EaeI y'GGCC_r

Cuts at:    113     185     654    1145    1412    2363    2537    2928    2955 
   Size:         72     469     491     267     951     174     391      27

Cuts at:   2955    4300     113 
   Size:       1345     591

  Fragments arranged by size: 

                1345     951     591     491     469     391     267     174
                  72      27
 
EagI C'GGCC_G

Cuts at:    113    2363     113 
   Size:       2250    2528
 
EarI CTCTTCn'nnn_

Cuts at:    175     751    1532    1687    2801    3011     175 
   Size:        576     781     155    1114     210    1942

  Fragments arranged by size: 

                1942    1114     781     576     210     155
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    274    2986    3296    3442    4588     274 
   Size:       2712     310     146    1146     464

  Fragments arranged by size: 

                2712    1146     464     310     146
 
EcoICRI GAG'CTC

Cuts at:   1790    1790 
   Size:       4778
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    262     783     963    1124    1753    1770    2603    3035    3782 
   Size:        521     180     161     629      17     833     432     747

Cuts at:   3782    4050     262 
   Size:        268     990

  Fragments arranged by size: 

                 990     833     747     629     521     432     268     180
                 161      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:    432     733     432 
   Size:        301    4477
 
EcoO109I rG'GnC_Cy

Cuts at:    459    1342    1917     459 
   Size:        883     575    3320

  Fragments arranged by size: 

                3320     883     575
 
EcoRI G'AATT_C

Cuts at:   1782    1782 
   Size:       4778
 
EcoRV GAT'ATC

Cuts at:    548     548 
   Size:       4778
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    372     795    2797     372 
   Size:        423    2002    2353

  Fragments arranged by size: 

                2353    2002     423
 
FspI TGC'GCA

Cuts at:   2559    2559 
   Size:       4778
 
HaeII r_GCGC'y

Cuts at:    752     867    1139    2212    2460    3482    4461     752 
   Size:        115     272    1073     248    1022     979    1069

  Fragments arranged by size: 

                1073    1069    1022     979     272     248     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    448     480     808     840    1477    1509    1531    1563    2620 
   Size:         32     328      32     637      32      22      32    1057

Cuts at:   2620    2650    2652    2682     448 
   Size:         30       2      30    2544

  Fragments arranged by size: 

                2544    1057     637     328      32      32      32      32
                  30      30      22       2
 
HincII GTy'rAC

Cuts at:   1200    1817    1200 
   Size:        617    4161
 
KasI G'GCGC_C

Cuts at:   2208    2456    2208 
   Size:        248    4530
 
KpnI G_GTAC'C

Cuts at:   1798    1798 
   Size:       4778
 
MluI A'CGCG_T

Cuts at:   4211    4211 
   Size:       4778
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3449    3633    3449 
   Size:        184    4594
 
MscI TGG'CCA

Cuts at:    187    1147    1414    2539     187 
   Size:        960     267    1125    2426

  Fragments arranged by size: 

                2426    1125     960     267
 
MslI CAynn'nnrTG

Cuts at:    307     364    2767    2894     307 
   Size:         57    2403     127    2191

  Fragments arranged by size: 

                2403    2191     127      57
 
MspA1I CmG'CkG

Cuts at:    901    1113    1172    1317    1889    2203    2563    3576    3821 
   Size:        212      59     145     572     314     360    1013     245

Cuts at:   3821     901 
   Size:       1858

  Fragments arranged by size: 

                1858    1013     572     360     314     245     212     145
                  59
 
NaeI GCC'GGC

Cuts at:   2959    2959 
   Size:       4778
 
NarI GG'CG_CC

Cuts at:   2209    2457    2209 
   Size:        248    4530
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4778
 
NgoMIV G'CCGG_C

Cuts at:   2957    2957 
   Size:       4778
 
NotI GC'GGCC_GC

Cuts at:    113     113 
   Size:       4778
 
NspI r_CATG'y

Cuts at:    999    1831    2331    3238     999 
   Size:        832     500     907    2539

  Fragments arranged by size: 

                2539     907     832     500
 
PciI A'CATG_T

Cuts at:   3234    3234 
   Size:       4778
 
PfoI T'CCnGG_A

Cuts at:   1730    1747    1730 
   Size:         17    4761
 
PmeI GTTT'AAAC

Cuts at:   1777    1777 
   Size:       4778
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3917    3949    3917 
   Size:         32    4746
 
PspOMI G'GGCC_C

Cuts at:    595     595 
   Size:       4778
 
PstI C_TGCA'G

Cuts at:   1825    2510    1825 
   Size:        685    4093
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4778
 
PvuII CAG'CTG

Cuts at:   1317    2203    2563    1317 
   Size:        886     360    3532

  Fragments arranged by size: 

                3532     886     360
 
RsrII CG'GwC_CG

Cuts at:   2973    2973 
   Size:       4778
 
SacI G_AGCT'C

Cuts at:   1792    1792 
   Size:       4778
 
SalI G'TCGA_C

Cuts at:   1815    1815 
   Size:       4778
 
SapI GCTCTTCn'nnn_

Cuts at:   2801    3011    2801 
   Size:        210    4568
 
SbfI CC_TGCA'GG

Cuts at:   1825    1825 
   Size:       4778
 
ScaI AGT'ACT

Cuts at:   4295    4295 
   Size:       4778
 
SfcI C'TryA_G

Cuts at:     33    1511    1821    2506    3499    3690      33 
   Size:       1478     310     685     993     191    1121

  Fragments arranged by size: 

                1478    1121     993     685     310     191
 
SfoI GGC'GCC

Cuts at:   2210    2458    2210 
   Size:        248    4530
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4778
 
SmaI CCC'GGG

Cuts at:    151    1800     151 
   Size:       1649    3129
 
SmlI C'TyrA_G

Cuts at:    769    1933    2848    3340    3602     769 
   Size:       1164     915     492     262    1945

  Fragments arranged by size: 

                1945    1164     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4778
 
SphI G_CATG'C

Cuts at:   1831    2331    1831 
   Size:        500    4278
 
StuI AGG'CCT

Cuts at:    427     640     427 
   Size:        213    4565
 
StyI C'CwwG_G

Cuts at:    107    1912    2266     107 
   Size:       1805     354    2619

  Fragments arranged by size: 

                2619    1805     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1385    2361    2494    2953    3162    1385 
   Size:        976     133     459     209    3001

  Fragments arranged by size: 

                3001     976     459     209     133
 
TatI w'GTAC_w

Cuts at:   1115    1502    4293    1115 
   Size:        387    2791    1600

  Fragments arranged by size: 

                2791    1600     387
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4295    4327    4295 
   Size:         32    4746
 
Tth111I GACn'n_nGTC

Cuts at:   2575    2575 
   Size:       4778
 
XbaI T'CTAG_A

Cuts at:   1809    1809 
   Size:       4778
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    740     987    2273     740 
   Size:        247    1286    3245

  Fragments arranged by size: 

                3245    1286     247
 
XmaI C'CCGG_G

Cuts at:    149    1798     149 
   Size:       1649    3129
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NotI     NspI     PciI     PfoI     PmeI     PpiI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AlfI     AloI     AscI    AvrII     BaeI
    BbvCI     BcgI    BfrBI     BmtI     BsgI    BsiWI    BsmBI   BstEII
  BstZ17I     ClaI    CspCI   DraIII     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NruI     NsiI     PacI     PasI    PflMI
     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com