Restriction map for pF1KB5443
 (Linear) (Six-base) MAPSORT of: KIBB5443.seq  Check: 355  from: 1  to: 579



KIBB5443.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     362     579 
   Size:        362     217
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     178     579 
   Size:        178     401
 
ApaI G_GGCC'C

Cuts at:      0      52     579 
   Size:         52     527
 
AvaI C'yCGr_G

Cuts at:      0     446     579 
   Size:        446     133
 
BanII G_rGCy'C

Cuts at:      0      52      80     579 
   Size:         52      28     499

  Fragments arranged by size: 

                 499      52      28
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     294     328     579 
   Size:        294      34     251

  Fragments arranged by size: 

                 294     251      34
 
BclI T'GATC_A

Cuts at:      0     245     579 
   Size:        245     334
 
BglI GCCn_nnn'nGGC

Cuts at:      0     184     579 
   Size:        184     395
 
Bme1580I G_kGCm'C

Cuts at:      0      52     145     579 
   Size:         52      93     434

  Fragments arranged by size: 

                 434      93      52
 
BmgBI CAC'GTC

Cuts at:      0     128     441     579 
   Size:        128     313     138

  Fragments arranged by size: 

                 313     138     128
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     520     579 
   Size:        520      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     109     532     579 
   Size:         34      75     423      47

  Fragments arranged by size: 

                 423      75      47      34
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490     579 
   Size:        490      89
 
BsaAI yAC'GTr

Cuts at:      0     351     579 
   Size:        351     228
 
BsaHI Gr'CG_yC

Cuts at:      0       8     579 
   Size:          8     571
 
BsaWI w'CCGG_w

Cuts at:      0     556     579 
   Size:        556      23
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      82     112     579 
   Size:         82      30     467

  Fragments arranged by size: 

                 467      82      30
 
BseYI C'CCAG_C

Cuts at:      0       3      27     579 
   Size:          3      24     552

  Fragments arranged by size: 

                 552      24       3
 
BsiEI CG_ry'CG

Cuts at:      0     495     579 
   Size:        495      84
 
BsiHKAI G_wGCw'C

Cuts at:      0     208     579 
   Size:        208     371
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      64     579 
   Size:         64     515
 
Bsp1286I G_dGCh'C

Cuts at:      0      52      80     145     208     579 
   Size:         52      28      65      63     371

  Fragments arranged by size: 

                 371      65      63      52      28
 
BspEI T'CCGG_A

Cuts at:      0     556     579 
   Size:        556      23
 
BsrBI CCG'CTC

Cuts at:      0     516     579 
   Size:        516      63
 
BsrFI r'CCGG_y

Cuts at:      0     408     579 
   Size:        408     171
 
BssSI C'ACGA_G

Cuts at:      0     214     579 
   Size:        214     365
 
BstEII G'GTnAC_C

Cuts at:      0     430     579 
   Size:        430     149
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      87     579 
   Size:         87     492
 
BstYI r'GATC_y

Cuts at:      0     329     579 
   Size:        329     250
 
BtgI C'CryG_G

Cuts at:      0      57     137     579 
   Size:         57      80     442

  Fragments arranged by size: 

                 442      80      57
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     297     579 
   Size:        297     282
 
BtsI GCAGTG_nn'

Cuts at:      0     143     579 
   Size:        143     436
 
EaeI y'GGCC_r

Cuts at:      0     406     579 
   Size:        406     173
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     109     532     579 
   Size:         34      75     423      47

  Fragments arranged by size: 

                 423      75      47      34
 
HaeII r_GCGC'y

Cuts at:      0     179     579 
   Size:        179     400
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     219     251     458     490     579 
   Size:        219      32     207      32      89

  Fragments arranged by size: 

                 219     207      89      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     155     579 
   Size:        143      12     424

  Fragments arranged by size: 

                 424     143      12
 
MslI CAynn'nnrTG

Cuts at:      0     160     382     579 
   Size:        160     222     197

  Fragments arranged by size: 

                 222     197     160
 
MspA1I CmG'CkG

Cuts at:      0      59     579 
   Size:         59     520
 
PasI CC'CwG_GG

Cuts at:      0     150     579 
   Size:        150     429
 
PfoI T'CCnGG_A

Cuts at:      0      11     579 
   Size:         11     568
 
PmlI CAC'GTG

Cuts at:      0     351     579 
   Size:        351     228
 
PspOMI G'GGCC_C

Cuts at:      0      48     579 
   Size:         48     531
 
SacII CC_GC'GG

Cuts at:      0      60     579 
   Size:         60     519
 
ScaI AGT'ACT

Cuts at:      0     544     579 
   Size:        544      35
 
SexAI A'CCwGG_T

Cuts at:      0     260     579 
   Size:        260     319
 
SfcI C'TryA_G

Cuts at:      0     333     579 
   Size:        333     246
 
SmaI CCC'GGG

Cuts at:      0     448     579 
   Size:        448     131
 
SmlI C'TyrA_G

Cuts at:      0     505     579 
   Size:        505      74
 
SpeI A'CTAG_T

Cuts at:      0     267     579 
   Size:        267     312
 
StyI C'CwwG_G

Cuts at:      0     179     579 
   Size:        179     400
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     509     579 
   Size:        509      70
 
TatI w'GTAC_w

Cuts at:      0     542     579 
   Size:        542      37
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      82     114     205     237     551     579 
   Size:         82      32      91      32     314      28

  Fragments arranged by size: 

                 314      91      82      32      32      28
 
XmaI C'CCGG_G

Cuts at:      0     446     579 
   Size:        446     133
 

 Enzymes that do cut:

     AccI     AlfI     ApaI     AvaI    BanII     BcgI     BclI     BglI
 Bme1580I    BmgBI     BplI     BpmI    BpuEI    BsaAI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BsrBI
    BsrFI    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     EaeI  Eco57MI    HaeII    Hin4I     MmeI     MslI   MspA1I     PasI
     PfoI     PmlI   PspOMI    SacII     ScaI    SexAI     SfcI     SmaI
     SmlI     SpeI     StyI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI    BciVI    BfrBI    BglII     BlpI     BmrI     BmtI   Bpu10I
     BsaI    BsaBI    BseRI     BsgI    BsiWI     BsmI    BspHI    BspMI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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