Restriction map for pF1KB5407
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BamHI       
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrGI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MaeI        
MamI        MfeI        MflI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        PvuII       
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AlwI          878  GGATC                GGATC(4/5)
AviII         884  TGCGCA               TGC^GCA
BalI          782  TGGCCA               TGG^CCA
BbsI         1009  GTCTTC               GAAGAC(2/6)
BglI          862  GCCGTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1009  GTCTTC               GAAGAC(2/6)
BsaAI         799  TACGTG               [CT]AC^GT[GA]
BsiEI         467  CGGCCG               CG[GA][CT]^CG
BspHI         761  TCATGA               T^CATGA
BsrFI        1397  GCCGGC               [GA]^CCGG[CT]
BssSI        1273  CACGAG               CACGAG(-5/-1)
Cfr10I       1397  GCCGGC               [GA]^CCGG[CT]
EagI          467  CGGCCG               C^GGCCG
EarI          721  CTCTTC               CTCTTC(1/4)
EclXI         467  CGGCCG               C^GGCCG
Eco52I        467  CGGCCG               C^GGCCG
EcoNI        1437  CCTGACCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1031  CCATGG               C^C[AT][AT]GG
FspI          884  TGCGCA               TGC^GCA
HincII         79  GTCAAC               GT[CT]^[GA]AC
HindII         79  GTCAAC               GT[CT]^[GA]AC
HinfI         526  GAGTC                G^A[ACGT]TC
Ksp632I       721  CTCTTC               CTCTTC(1/4)
KspI         1262  CCGCGG               CCGC^GG
MluNI         782  TGGCCA               TGG^CCA
MscI          782  TGGCCA               TGG^CCA
MseI         1061  TTAA                 T^TAA
NaeI         1397  GCCGGC               GCC^GGC
NcoI         1031  CCATGG               C^CATGG
NgoMIV       1397  GCCGGC               G^CCGGC
PleI          526  GAGTC                GAGTC(4/5)
PshAI        1104  GACCACCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          761  TCATGA               T^CATGA
SacII        1262  CCGCGG               CCGC^GG
StyI         1031  CCATGG               C^C[AT][AT]GG
TaqI          269  TCGA                 T^CGA
Tru9I        1061  TTAA                 T^TAA
TthHB8I       269  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BbeI          482  GGCGCC               GGCGC^C
             1380  GGCGCC              
BsmBI         579  CGTCTC               CGTCTC(1/5)
              676  GAGACG              
BsmFI         113  GGGAC                GGGAC(10/14)
              242  GGGAC               
BsrI          969  ACTGG                ACTGG(1/-1)
             1074  CCAGT               
BssHII        424  GCGCGC               G^CGCGC
              892  GCGCGC              
BstAPI        858  GCAGGCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              867  GCAGGCGCTGC         
DpnII         420  GATC                 ^GATC
              879  GATC                
KasI          482  GGCGCC               G^GCGCC
             1380  GGCGCC              
MaeIII        729  GTCAC                ^GT[ACGT]AC
             1340  GTGAC               
MboI          420  GATC                 ^GATC
              879  GATC                
NarI          482  GGCGCC               GG^CGCC
             1380  GGCGCC              
NdeII         420  GATC                 ^GATC
              879  GATC                
SacI           45  GAGCTC               GAGCT^C
              151  GAGCTC              
Sau3AI        420  GATC                 ^GATC
              879  GATC                
SfoI          482  GGCGCC               GGC^GCC
             1380  GGCGCC              
SmlI          358  CTCAAG               C^T[CT][GA]AG
              949  CTCAAG              
Tsp45I        729  GTCAC                ^GT[GC]AC
             1340  GTGAC               
TspRI         995  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1338  CAGTG               
XcmI         1133  CCAAGACCACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1268  CCAAGCACGAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BspMI       BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        MaeII       MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
PpuMI       PstI        RsaI        Sau96I      ScrFI       
SfaNI       SfcI        TseI        
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