Restriction map for pF1KB4568
 (Circular) (Six-base) MAPSORT of: pF1KB4568.seq  Check: 2531  from: 1  to: 4223



pF1KB4568  4223 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:   1261    1261 
   Size:       4223
 
Acc65I G'GTAC_C

Cuts at:   1239    1239 
   Size:       4223
 
AclI AA'CG_TT

Cuts at:    525    4063     525 
   Size:       3538     685
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2048    2480    3227    3495    2048 
   Size:        432     747     268    2776

  Fragments arranged by size: 

                2776     747     432     268
 
AflIII A'CryG_T

Cuts at:   1575    2679    3656    1575 
   Size:       1104     977    2142

  Fragments arranged by size: 

                2142    1104     977
 
AgeI A'CCGG_T

Cuts at:   2611    2611 
   Size:       4223
 
AleI CACnn'nnGTG

Cuts at:    625     625 
   Size:       4223
 
AlwNI CAG_nnn'CTG

Cuts at:   1528    3095    1528 
   Size:       1567    2656
 
ApaLI G'TGCA_C

Cuts at:   2993    2993 
   Size:       4223
 
ApoI r'AATT_y

Cuts at:    886    1227    3576    3832     886 
   Size:        341    2349     256    1277

  Fragments arranged by size: 

                2349    1277     341     256
 
AseI AT'TA_AT

Cuts at:     20     404      20 
   Size:        384    3839
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4223
 
AvaI C'yCGr_G

Cuts at:   1243    1243 
   Size:       4223
 
BamHI G'GATC_C

Cuts at:    374    1248     374 
   Size:        874    3349
 
BanI G'GyrC_C

Cuts at:    293     569     583    1239    1653    1901    1936     293 
   Size:        276      14     656     414     248      35    2580

  Fragments arranged by size: 

                2580     656     414     276     248      35      14
 
BanII G_rGCy'C

Cuts at:    265     451    1237    2267     265 
   Size:        186     786    1030    2221

  Fragments arranged by size: 

                2221    1030     786     186
 
BbeI G_GCGC'C

Cuts at:    297     573    1657    1905     297 
   Size:        276    1084     248    2615

  Fragments arranged by size: 

                2615    1084     276     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    345     345 
   Size:       4223
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4189
 
BciVI GTATCCnnnnn_n'

Cuts at:   2121    2882    4223    2121 
   Size:        761    1341    2121

  Fragments arranged by size: 

                2121    1341     761
 
BclI T'GATC_A

Cuts at:   1743    1743 
   Size:       4223
 
BglI GCCn_nnn'nGGC

Cuts at:    139     314     334     139 
   Size:        175      20    4028

  Fragments arranged by size: 

                4028     175      20
 
BglII A'GATC_T

Cuts at:    397    1738    3775     397 
   Size:       1341    2037     845

  Fragments arranged by size: 

                2037    1341     845
 
BlpI GC'TnA_GC

Cuts at:   1121    1335    1121 
   Size:        214    4009
 
Bme1580I G_kGCm'C

Cuts at:   1848    1941    2997    1848 
   Size:         93    1056    3074

  Fragments arranged by size: 

                3074    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    759    1435    1516    1851    3995     759 
   Size:        676      81     335    2144     987

  Fragments arranged by size: 

                2144     987     676     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2590    2590 
   Size:       4223
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    455     473     724     866    1072     455 
   Size:         18     251     142     206    3606

  Fragments arranged by size: 

                3606     251     206     142      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1399    2278    2770    3068    1399 
   Size:        879     492     298    2554

  Fragments arranged by size: 

                2554     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3927    3927 
   Size:       4223
 
BsaAI yAC'GTr

Cuts at:   1576    1576 
   Size:       4223
 
BsaBI GATnn'nnATC

Cuts at:      6    1737    3774       6 
   Size:       1731    2037     455

  Fragments arranged by size: 

                2037    1731     455
 
BsaHI Gr'CG_yC

Cuts at:    294     350     570    1654    1902    2604    4100     294 
   Size:         56     220    1084     248     702    1496     417

  Fragments arranged by size: 

                1496    1084     702     417     248     220      56
 
BsaWI w'CCGG_w

Cuts at:   1412    1933    2611    2885    3032    3443    1412 
   Size:        521     678     274     147     411    2192

  Fragments arranged by size: 

                2192     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    373     403     373 
   Size:         30    4193
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    268     382     397     400     268 
   Size:        114      15       3    4091

  Fragments arranged by size: 

                4091     114      15       3
 
BseYI C'CCAG_C

Cuts at:   1648    2406    2983    3479    1648 
   Size:        758     577     496    2392

  Fragments arranged by size: 

                2392     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    286    1045     286 
   Size:        759    3464
 
BsiEI CG_ry'CG

Cuts at:    104     246     285     309    1765    1811    3019     104 
   Size:        142      39      24    1456      46    1208    1308

  Fragments arranged by size: 

                1456    1308    1208     142      46      39      24
 
BsiHKAI G_wGCw'C

Cuts at:    265     451    1237    2015    2205    2997     265 
   Size:        186     786     778     190     792    1491

  Fragments arranged by size: 

                1491     792     786     778     190     186
 
Bsp1286I G_dGCh'C

Cuts at:    265     451    1237    1848    1941    2015    2205    2267    2997 
   Size:        186     786     611      93      74     190      62     730

Cuts at:   2997     265 
   Size:       1491

  Fragments arranged by size: 

                1491     786     730     611     190     186      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3443    3443 
   Size:       4223
 
BspHI T'CATG_A

Cuts at:   3399    3399 
   Size:       4223
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1273    1487    1789    2170    1273 
   Size:        214     302     381    3326

  Fragments arranged by size: 

                3326     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2515    2569    4053    4218      50 
   Size:       2465      54    1484     165      55

  Fragments arranged by size: 

                2465    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2135    2135 
   Size:       4223
 
BsrFI r'CCGG_y

Cuts at:    296     346    2221    2402    2611     296 
   Size:         50    1875     181     209    1908

  Fragments arranged by size: 

                1908    1875     209     181      50
 
BssHII G'CGCG_C

Cuts at:    216     218     216 
   Size:          2    4221
 
BssSI C'ACGA_G

Cuts at:   2494    2852    2494 
   Size:        358    3865
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1600    1600 
   Size:       4223
 
BstBI TT'CG_AA

Cuts at:   2584    2584 
   Size:       4223
 
BstEII G'GTnAC_C

Cuts at:    753     753 
   Size:       4223
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1089    1089 
   Size:       4223
 
BstYI r'GATC_y

Cuts at:      1     374     397    1248    1715    1738    2073    2319    3331 
   Size:        373      23     851     467      23     335     246    1012

Cuts at:   3331    3417    3429    3775       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     851     467     449     373     346     335     246
                  86      23      23      12
 
BtgI C'CryG_G

Cuts at:    115     115 
   Size:       4223
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2173    2354    2517    2173 
   Size:        181     163    3879

  Fragments arranged by size: 

                3879     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1466    1466 
   Size:       4223
 
DraI TTT'AAA

Cuts at:   1222    1222 
   Size:       4223
 
DrdI GACnn_nn'nnGTC

Cuts at:   1929    2787    1929 
   Size:        858    3365
 
EaeI y'GGCC_r

Cuts at:    128     176     243     282     306     315    1067    1808    1982 
   Size:         48      67      39      24       9     752     741     174

Cuts at:   1982    2373    2400    3745     128 
   Size:        391      27    1345     606

  Fragments arranged by size: 

                1345     752     741     606     391     174      67      48
                  39      27      24       9
 
EagI C'GGCC_G

Cuts at:    243     282     306    1808     243 
   Size:         39      24    1502    2658

  Fragments arranged by size: 

                2658    1502      39      24
 
EarI CTCTTCn'nnn_

Cuts at:   2246    2456    2246 
   Size:        210    4013
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2431    2741    2887    4033    2431 
   Size:        310     146    1146    2621

  Fragments arranged by size: 

                2621    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    263     449    1235     263 
   Size:        186     786    3251

  Fragments arranged by size: 

                3251     786     186
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    455     473     724     866    1072    2048    2480    3227    3495 
   Size:         18     251     142     206     976     432     747     268

Cuts at:   3495     455 
   Size:       1183

  Fragments arranged by size: 

                1183     976     747     432     268     251     206     142
                  18
 
EcoO109I rG'GnC_Cy

Cuts at:    159    1362     159 
   Size:       1203    3020
 
EcoRI G'AATT_C

Cuts at:    886    1227     886 
   Size:        341    3882
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    570    2242     570 
   Size:       1672    2551
 
FspI TGC'GCA

Cuts at:   2004    2004 
   Size:       4223
 
HaeII r_GCGC'y

Cuts at:    172     297     573    1657    1905    2927    3906     172 
   Size:        125     276    1084     248    1022     979     489

  Fragments arranged by size: 

                1084    1022     979     489     276     248     125
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1099    1131    2065    2095    2097    2127    1099 
   Size:         32     934      30       2      30    3195

  Fragments arranged by size: 

                3195     934      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1262    1262 
   Size:       4223
 
KasI G'GCGC_C

Cuts at:    293     569    1653    1901     293 
   Size:        276    1084     248    2615

  Fragments arranged by size: 

                2615    1084     276     248
 
KpnI G_GTAC'C

Cuts at:   1243    1243 
   Size:       4223
 
MluI A'CGCG_T

Cuts at:   3656    3656 
   Size:       4223
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    401    1000    2894    3078     401 
   Size:        599    1894     184    1546

  Fragments arranged by size: 

                1894    1546     599     184
 
MscI TGG'CCA

Cuts at:    178     317    1069    1984     178 
   Size:        139     752     915    2417

  Fragments arranged by size: 

                2417     915     752     139
 
MslI CAynn'nnrTG

Cuts at:    625     835     866    2212    2339     625 
   Size:        210      31    1346     127    2509

  Fragments arranged by size: 

                2509    1346     210     127      31
 
MspA1I CmG'CkG

Cuts at:    117     657    1190    1334    1648    2008    3021    3266     117 
   Size:        540     533     144     314     360    1013     245    1074

  Fragments arranged by size: 

                1074    1013     540     533     360     314     245     144
            
 
NaeI GCC'GGC

Cuts at:    298     348    2404     298 
   Size:         50    2056    2117

  Fragments arranged by size: 

                2117    2056      50
 
NarI GG'CG_CC

Cuts at:    294     570    1654    1902     294 
   Size:        276    1084     248    2615

  Fragments arranged by size: 

                2615    1084     276     248
 
NdeI CA'TA_TG

Cuts at:    906     906 
   Size:       4223
 
NgoMIV G'CCGG_C

Cuts at:    296     346    2402     296 
   Size:         50    2056    2117

  Fragments arranged by size: 

                2117    2056      50
 
NspI r_CATG'y

Cuts at:   1041    1276    1776    2683    1041 
   Size:        235     500     907    2581

  Fragments arranged by size: 

                2581     907     500     235
 
PciI A'CATG_T

Cuts at:   2679    2679 
   Size:       4223
 
PfoI T'CCnGG_A

Cuts at:    377    1049     377 
   Size:        672    3551
 
PmeI GTTT'AAAC

Cuts at:   1222    1222 
   Size:       4223
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3362    3394    3362 
   Size:         32    4191
 
PstI C_TGCA'G

Cuts at:   1270    1955    1270 
   Size:        685    3538
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4223
 
PvuII CAG'CTG

Cuts at:    657    1190    1648    2008     657 
   Size:        533     458     360    2872

  Fragments arranged by size: 

                2872     533     458     360
 
RsrII CG'GwC_CG

Cuts at:   2418    2418 
   Size:       4223
 
SacI G_AGCT'C

Cuts at:    265     451    1237     265 
   Size:        186     786    3251

  Fragments arranged by size: 

                3251     786     186
 
SacII CC_GC'GG

Cuts at:    118     118 
   Size:       4223
 
SalI G'TCGA_C

Cuts at:   1260    1260 
   Size:       4223
 
SapI GCTCTTCn'nnn_

Cuts at:   2246    2456    2246 
   Size:        210    4013
 
SbfI CC_TGCA'GG

Cuts at:   1270    1270 
   Size:       4223
 
ScaI AGT'ACT

Cuts at:   3740    3740 
   Size:       4223
 
SfcI C'TryA_G

Cuts at:     33    1266    1951    2944    3135      33 
   Size:       1233     685     993     191    1121

  Fragments arranged by size: 

                1233    1121     993     685     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    314     314 
   Size:       4223
 
SfoI GGC'GCC

Cuts at:    295     571    1655    1903     295 
   Size:        276    1084     248    2615

  Fragments arranged by size: 

                2615    1084     276     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4223
 
SmaI CCC'GGG

Cuts at:   1245    1245 
   Size:       4223
 
SmlI C'TyrA_G

Cuts at:   1378    2293    2785    3047    1378 
   Size:        915     492     262    2554

  Fragments arranged by size: 

                2554     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4223
 
SphI G_CATG'C

Cuts at:   1041    1276    1776    1041 
   Size:        235     500    3488

  Fragments arranged by size: 

                3488     500     235
 
StyI C'CwwG_G

Cuts at:   1148    1357    1711    1148 
   Size:        209     354    3660

  Fragments arranged by size: 

                3660     354     209
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    601    1806    1939    2398    2607     601 
   Size:       1205     133     459     209    2217

  Fragments arranged by size: 

                2217    1205     459     209     133
 
TatI w'GTAC_w

Cuts at:    776    3738     776 
   Size:       2962    1261
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3740    3772    3740 
   Size:         32    4191
 
Tth111I GACn'n_nGTC

Cuts at:   2020    2020 
   Size:       4223
 
XbaI T'CTAG_A

Cuts at:   1254    1254 
   Size:       4223
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1718    1718 
   Size:       4223
 
XmaI C'CCGG_G

Cuts at:   1243    1243 
   Size:       4223
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I
    BsiWI     BsmI    BsmBI    BsrGI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SanDI
    SexAI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com