Restriction map for pF1KB3573
 (Linear) (Six-base) MAPSORT of: KIBB3573.seq  Check: 9781  from: 1  to: 1608



KIBB3573.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:      0    1310    1328    1608 
   Size:       1310      18     280

  Fragments arranged by size: 

                1310     280      18
 
Acc65I G'GTAC_C

Cuts at:      0     220     477    1052    1608 
   Size:        220     257     575     556

  Fragments arranged by size: 

                 575     556     257     220
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     210     467     540    1368    1608 
   Size:         76     134     257      73     828     240

  Fragments arranged by size: 

                 828     257     240     134      76      73
 
AflIII A'CryG_T

Cuts at:      0    1343    1608 
   Size:       1343     265
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     325    1608 
   Size:        325    1283
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     323     355    1608 
   Size:        323      32    1253

  Fragments arranged by size: 

                1253     323      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     379     650     861    1608 
   Size:        379     271     211     747

  Fragments arranged by size: 

                 747     379     271     211
 
ApaI G_GGCC'C

Cuts at:      0     281    1608 
   Size:        281    1327
 
ApoI r'AATT_y

Cuts at:      0     420    1608 
   Size:        420    1188
 
AvrII C'CTAG_G

Cuts at:      0     906    1086    1608 
   Size:        906     180     522

  Fragments arranged by size: 

                 906     522     180
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1036    1069    1608 
   Size:       1036      33     539

  Fragments arranged by size: 

                1036     539      33
 
BamHI G'GATC_C

Cuts at:      0    1536    1608 
   Size:       1536      72
 
BanI G'GyrC_C

Cuts at:      0     115     220     477    1052    1608 
   Size:        115     105     257     575     556

  Fragments arranged by size: 

                 575     556     257     115     105
 
BanII G_rGCy'C

Cuts at:      0     281     609    1150    1408    1608 
   Size:        281     328     541     258     200

  Fragments arranged by size: 

                 541     328     281     258     200
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1462    1496    1608 
   Size:       1462      34     112

  Fragments arranged by size: 

                1462     112      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     838    1608 
   Size:        838     770
 
BfrBI ATG'CAT

Cuts at:      0    1467    1608 
   Size:       1467     141
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1135    1608 
   Size:       1135     473
 
BlpI GC'TnA_GC

Cuts at:      0     779    1608 
   Size:        779     829
 
Bme1580I G_kGCm'C

Cuts at:      0     120     281     304    1608 
   Size:        120     161      23    1304

  Fragments arranged by size: 

                1304     161     120      23
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     996    1608 
   Size:        996     612
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1581    1608 
   Size:       1581      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1055    1576    1608 
   Size:       1055     521      32

  Fragments arranged by size: 

                1055     521      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568     655     691    1608 
   Size:        568      87      36     917

  Fragments arranged by size: 

                 917     568      87      36
 
BsaHI Gr'CG_yC

Cuts at:      0    1599    1608 
   Size:       1599       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     172     202    1608 
   Size:        172      30    1406

  Fragments arranged by size: 

                1406     172      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1497    1608 
   Size:       1497     111
 
BseYI C'CCAG_C

Cuts at:      0     620     829    1608 
   Size:        620     209     779

  Fragments arranged by size: 

                 779     620     209
 
BsiHKAI G_wGCw'C

Cuts at:      0     785    1608 
   Size:        785     823
 
Bsp1286I G_dGCh'C

Cuts at:      0     120     281     304     609     785    1150    1408    1608 
   Size:        120     161      23     305     176     365     258     200

  Fragments arranged by size: 

                 365     305     258     200     176     161     120      23
            
 
BsrBI CCG'CTC

Cuts at:      0     685    1505    1608 
   Size:        685     820     103

  Fragments arranged by size: 

                 820     685     103
 
BsrDI GCAATG_nn'

Cuts at:      0     177     893    1037    1608 
   Size:        177     716     144     571

  Fragments arranged by size: 

                 716     571     177     144
 
BsrGI T'GTAC_A

Cuts at:      0    1044    1608 
   Size:       1044     564
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     115    1170    1608 
   Size:        115    1055     438

  Fragments arranged by size: 

                1055     438     115
 
BstYI r'GATC_y

Cuts at:      0    1536    1608 
   Size:       1536      72
 
Bsu36I CC'TnA_GG

Cuts at:      0    1400    1608 
   Size:       1400     208
 
BtgI C'CryG_G

Cuts at:      0     482    1608 
   Size:        482    1126
 
BtsI GCAGTG_nn'

Cuts at:      0     104     255     809    1460    1608 
   Size:        104     151     554     651     148

  Fragments arranged by size: 

                 651     554     151     148     104
 
DraIII CAC_nnn'GTG

Cuts at:      0     250    1608 
   Size:        250    1358
 
EaeI y'GGCC_r

Cuts at:      0     485    1377    1608 
   Size:        485     892     231

  Fragments arranged by size: 

                 892     485     231
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     934    1608 
   Size:        934     674
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     210     467     540    1055    1368    1576    1608 
   Size:         76     134     257      73     515     313     208      32

  Fragments arranged by size: 

                 515     313     257     208     134      76      73      32
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     565    1608 
   Size:        565    1043
 
EcoO109I rG'GnC_Cy

Cuts at:      0     277     910    1285    1608 
   Size:        277     633     375     323

  Fragments arranged by size: 

                 633     375     323     277
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     452     484    1608 
   Size:        452      32    1124

  Fragments arranged by size: 

                1124     452      32
 
HincII GTy'rAC

Cuts at:      0    1311    1608 
   Size:       1311     297
 
HindIII A'AGCT_T

Cuts at:      0     138    1608 
   Size:        138    1470
 
KpnI G_GTAC'C

Cuts at:      0     224     481    1056    1608 
   Size:        224     257     575     552

  Fragments arranged by size: 

                 575     552     257     224
 
MfeI C'AATT_G

Cuts at:      0    1290    1608 
   Size:       1290     318
 
MscI TGG'CCA

Cuts at:      0     487    1379    1608 
   Size:        487     892     229

  Fragments arranged by size: 

                 892     487     229
 
MslI CAynn'nnrTG

Cuts at:      0     938    1472    1608 
   Size:        938     534     136

  Fragments arranged by size: 

                 938     534     136
 
MspA1I CmG'CkG

Cuts at:      0     491     811    1608 
   Size:        491     320     797

  Fragments arranged by size: 

                 797     491     320
 
NcoI C'CATG_G

Cuts at:      0     482    1608 
   Size:        482    1126
 
NsiI A_TGCA'T

Cuts at:      0    1469    1608 
   Size:       1469     139
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     650     861    1157    1416    1608 
   Size:        650     211     296     259     192

  Fragments arranged by size: 

                 650     296     259     211     192
 
PfoI T'CCnGG_A

Cuts at:      0     764    1553    1608 
   Size:        764     789      55

  Fragments arranged by size: 

                 789     764      55
 
PpuMI rG'GwC_Cy

Cuts at:      0     910    1285    1608 
   Size:        910     375     323

  Fragments arranged by size: 

                 910     375     323
 
PshAI GACnn'nnGTC

Cuts at:      0     908    1608 
   Size:        908     700
 
PspOMI G'GGCC_C

Cuts at:      0     277    1608 
   Size:        277    1331
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1216    1248    1608 
   Size:       1216      32     360

  Fragments arranged by size: 

                1216     360      32
 
PstI C_TGCA'G

Cuts at:      0     326     614    1608 
   Size:        326     288     994

  Fragments arranged by size: 

                 994     326     288
 
PvuII CAG'CTG

Cuts at:      0     491     811    1608 
   Size:        491     320     797

  Fragments arranged by size: 

                 797     491     320
 
SalI G'TCGA_C

Cuts at:      0    1309    1608 
   Size:       1309     299
 
ScaI AGT'ACT

Cuts at:      0     930    1608 
   Size:        930     678
 
SfcI C'TryA_G

Cuts at:      0     147     322     610    1608 
   Size:        147     175     288     998

  Fragments arranged by size: 

                 998     288     175     147
 
SmlI C'TyrA_G

Cuts at:      0     583     670     706    1608 
   Size:        583      87      36     902

  Fragments arranged by size: 

                 902     583      87      36
 
StuI AGG'CCT

Cuts at:      0     308    1138    1608 
   Size:        308     830     470

  Fragments arranged by size: 

                 830     470     308
 
StyI C'CwwG_G

Cuts at:      0     216     482     906    1086    1337    1374    1608 
   Size:        216     266     424     180     251      37     234

  Fragments arranged by size: 

                 424     266     251     234     216     180      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     675    1267    1284    1608 
   Size:        675     592      17     324

  Fragments arranged by size: 

                 675     592     324      17
 
TatI w'GTAC_w

Cuts at:      0     241     928    1044    1202    1608 
   Size:        241     687     116     158     406

  Fragments arranged by size: 

                 687     406     241     158     116
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AlfI     AloI    AlwNI     ApaI
     ApoI    AvrII     BaeI    BamHI     BanI    BanII     BcgI    BciVI
    BfrBI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI
    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrBI    BsrDI
    BsrGI   BstAPI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI
     EciI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII  HindIII     KpnI
     MfeI     MscI     MslI   MspA1I     NcoI     NsiI    PflMI     PfoI
    PpuMI    PshAI   PspOMI     PsrI     PstI    PvuII     SalI     ScaI
     SfcI     SmlI     StuI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI
    ApaLI     AscI     AseI    AsiSI     AvaI     BbeI     BbsI    BbvCI
     BclI    BglII    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EarI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI     PsiI    PspXI     PvuI    RsrII     SacI    SacII    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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