Restriction map for pF1KB3382
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AgeI        Alw44I      AlwI        AlwNI       
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BcgI        BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstYI       BstZ17I     CelII       
ClaI        CpoI        DpnII       DraI        DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MboI        MfeI        
MflI        MluI        MseI        MunI        MvnI        
NarI        NcoI        NdeI        NdeII       NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfcI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tth111I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII        195  TCCGGA               T^CCGGA
AcsI          870  GAATTC               [GA]^AATT[CT]
AcyI         1150  GACGCC               G[GA]^CG[CT]C
AflIII        779  ACGTGT               A^C[GA][CT]GT
AhdI          665  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          533  CCTCAGG              CC^T[ACGT]AGG
ApoI          870  GAATTC               [GA]^AATT[CT]
AspEI         665  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI          418  GTCTTC               GAAGAC(2/6)
BciVI         187  GTATCC               GTATCC(6/5)
BpuAI         418  GTCTTC               GAAGAC(2/6)
BsaHI        1150  GACGCC               G[GA]^CG[CT]C
BsaI           72  GGTCTC               GGTCTC(1/5)
BsaWI         195  TCCGGA               [AT]^CCGG[AT]
BseAI         195  TCCGGA               T^CCGGA
BspEI         195  TCCGGA               T^CCGGA
BspMI         498  GCAGGT               ACCTGC(4/8)
BsrDI         278  GCAATG               GCAATG(2/0)
BstEII        571  GGTGACC              G^GT[ACGT]ACC
BstPI         571  GGTGACC              G^GT[ACGT]ACC
BstXI         807  CCATCAGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        533  CCTCAGG              CC^T[ACGT]AGG
CfoI           12  GCGC                 GCG^C
DsaI          639  CCGTGG               C^C[GA][CT]GG
Eam1105I      665  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        533  CCTCAGG              CC^T[ACGT]AGG
EcoNI         981  CCTTGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       571  GGTGACC              G^GT[ACGT]ACC
EcoRI         870  GAATTC               G^AATTC
HgaI         1150  GACGC                GACGC(5/10)
HhaI           12  GCGC                 GCG^C
Hin1I        1150  GACGCC               G[GA]^CG[CT]C
HinP1I         12  GCGC                 G^CGC
MroI          195  TCCGGA               T^CCGGA
MslI          306  CACATCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          227  GCATGC               [GA]CATG^[CT]
PflMI         878  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          665  GACTC                GAGTC(4/5)
SphI          227  GCATGC               GCATG^C
StuI          654  AGGCCT               AGG^CCT
TaqI          659  TCGA                 T^CGA
Tsp509I       871  AATT                 ^AATT
TspRI         311  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       659  TCGA                 T^CGA
Van91I        878  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BalI          255  TGGCCA               TGG^CCA
              903  TGGCCA              
BbrPI         715  CACGTG               CAC^GTG
              778  CACGTG              
BbvCI         214  GCTGAGG              CCTCAGC(-5/-2)
              994  GCTGAGG             
BfaI          118  CTAG                 C^TAG
              610  CTAG                
BglI          605  GCCAGCTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              647  GCCGGCAAGGC         
BsaAI         715  CACGTG               [CT]AC^GT[GA]
              778  CACGTG              
BseRI        1041  CTCCTC               GAGGAG(10/8)
              997  GAGGAG              
BsrFI         177  GCCGGC               [GA]^CCGG[CT]
              647  GCCGGC              
Cfr10I        177  GCCGGC               [GA]^CCGG[CT]
              647  GCCGGC              
EarI          480  GAAGAG               CTCTTC(1/4)
             1002  GAAGAG              
FauI          593  GCGGG                CCCGC(4/6)
             1217  GCGGG               
Ksp632I       480  GAAGAG               CTCTTC(1/4)
             1002  GAAGAG              
MaeI          118  CTAG                 C^TAG
              610  CTAG                
MluNI         255  TGGCCA               TGG^CCA
              903  TGGCCA              
MscI          255  TGGCCA               TGG^CCA
              903  TGGCCA              
NaeI          177  GCCGGC               GCC^GGC
              647  GCCGGC              
NgoMIV        177  GCCGGC               G^CCGGC
              647  GCCGGC              
PmaCI         715  CACGTG               CAC^GTG
              778  CACGTG              
PmlI          715  CACGTG               CAC^GTG
              778  CACGTG              
PpuMI          28  AGGTCCC              [GA]G^G[AT]CC[CT]
               82  AGGTCCC             
SapI          480  GAAGAGC              GCTCTTC(1/4)
             1002  GAAGAGC             
SfiI          604  GGCCAGCTAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              646  GGCCGGCAAGGCC       
XcmI          878  CCAAGTCCTGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1118  CCAACTACCAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DraII       DraIII      
EaeI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PvuII       RsaI        
Sau96I      ScrFI       SfaNI       StyI        TfiI        
TseI        Tsp45I      
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