Restriction map for pF1KE0914
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AvaII       AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BsrI        BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
Eam1105I    EarI        Eco47III    Eco81I      EcoNI       
EcoO109I    EcoRI       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       PvuI        RcaI        RsrII       
SacI        SalI        SapI        Sau96I      ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
TspRI       Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AclI           10  AACGTT               AA^CGTT
AcsI          154  AAATTC               [GA]^AATT[CT]
AcyI          129  GGCGCC               G[GA]^CG[CT]C
AfaI          528  GTAC                 GT^AC
AlwI          537  GGATC                GGATC(4/5)
ApoI          154  AAATTC               [GA]^AATT[CT]
Asp700I       150  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         715  GTGCTC               G[AT]GC[AT]^C
AspI          678  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          703  CCCGAG               C^[CT]CG[GA]G
BanI          129  GGCGCC               G^G[CT][GA]CC
BbeI          129  GGCGCC               GGCGC^C
BbsI          580  GTCTTC               GAAGAC(2/6)
BfaI          188  CTAG                 C^TAG
BpuAI         580  GTCTTC               GAAGAC(2/6)
BsaHI         129  GGCGCC               G[GA]^CG[CT]C
BsaI          364  GAGACC               GGTCTC(1/5)
BsgI           55  GTGCAG               GTGCAG(16/14)
BsiHKAI       715  GTGCTC               G[AT]GC[AT]^C
BsmBI         432  CGTCTC               CGTCTC(1/5)
BsmFI         183  GTCCC                GGGAC(10/14)
BsoBI         703  CCCGAG               C^[CT]CG[GA]G
BspMI         530  ACCTGC               ACCTGC(4/8)
BssHII        463  GCGCGC               G^CGCGC
BstEII        303  GGTGACC              G^GT[ACGT]ACC
BstF5I        387  CATCC                GGATG(2/0)
BstPI         303  GGTGACC              G^GT[ACGT]ACC
BstYI         537  GGATCT               [GA]^GATC[CT]
DpnII         538  GATC                 ^GATC
EcoO65I       303  GGTGACC              G^GT[ACGT]ACC
EcoRV         142  GATATC               GAT^ATC
FokI          387  CATCC                GGATG(9/13)
HgaI          506  GCGTC                GACGC(5/10)
Hin1I         129  GGCGCC               G[GA]^CG[CT]C
KasI          129  GGCGCC               G^GCGCC
KspI          495  CCGCGG               CCGC^GG
MaeI          188  CTAG                 C^TAG
MboI          538  GATC                 ^GATC
MflI          537  GGATCT               [GA]^GATC[CT]
NarI          129  GGCGCC               GG^CGCC
NdeII         538  GATC                 ^GATC
NlaIV         129  GGCGCC               GG[ACGT]^[ACGT]CC
PflFI         678  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I       10  AACGTT               AA^CGTT
PvuII         292  CAGCTG               CAG^CTG
RsaI          528  GTAC                 GT^AC
SacII         495  CCGCGG               CCGC^GG
Sau3AI        538  GATC                 ^GATC
SbfI          531  CCTGCAGG             CCTGCA^GG
SfaNI         563  GCATC                GCATC(5/9)
SfoI          129  GGCGCC               GGC^GCC
Sse8387I      531  CCTGCAGG             CCTGCA^GG
TfiI          661  GATTC                G^A[AT]TC
Tsp509I       155  AATT                 ^AATT
Tth111I       678  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         537  GGATCT               [GA]^GATC[CT]
XmnI          150  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         464  CGCG                 CG^CG
              496  CGCG                
AlwNI         504  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              535  CAGGATCTG           
BanII         202  GGGCTC               G[GA]GC[CT]^C
              706  GAGCCC              
BsiEI         284  CGGCCG               CG[GA][CT]^CG
              416  CGGCCG              
BsiYI         490  CCCGCCCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              570  CCCCTCGGTGG         
BslI          490  CCCGCCCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              570  CCCCTCGGTGG         
BsrBI         195  CCGCTC               CCGCTC(-3/-3)
              405  GAGCGG              
BstUI         464  CGCG                 CG^CG
              496  CGCG                
DsaI          281  CCACGG               C^C[GA][CT]GG
              495  CCGCGG              
EagI          284  CGGCCG               C^GGCCG
              416  CGGCCG              
EclXI         284  CGGCCG               C^GGCCG
              416  CGGCCG              
Eco52I        284  CGGCCG               C^GGCCG
              416  CGGCCG              
MaeII          11  ACGT                 A^CGT
              221  ACGT                
MaeIII        229  GTCAC                ^GT[ACGT]AC
              304  GTGAC               
MvnI          464  CGCG                 CG^CG
              496  CGCG                
NlaIII        327  CATG                 CATG^
              721  CATG                
PstI          118  CTGCAG               CTGCA^G
              532  CTGCAG              
SfcI          118  CTGCAG               C^T[GA][CT]AG
              532  CTGCAG              
Tsp45I        229  GTCAC                ^GT[GC]AC
              304  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BmyI        
BpmI        BsaJI       BseRI       BsmAI       Bsp1286I    
BssKI       BstNI       Cac8I       CfoI        DdeI        
EaeI        EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
PleI        ScrFI       TaqI        TseI        TthHB8I     
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