Restriction map for pF1KE0914
 (Linear) (Six-base) MAPSORT of: KIEE0914.seq  Check: 7632  from: 1  to: 771



KIEE0914.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AclI AA'CG_TT

Cuts at:      0      11     771 
   Size:         11     760
 
AlwNI CAG_nnn'CTG

Cuts at:      0     509     540     771 
   Size:        509      31     231

  Fragments arranged by size: 

                 509     231      31
 
ApoI r'AATT_y

Cuts at:      0     154     771 
   Size:        154     617
 
AvaI C'yCGr_G

Cuts at:      0     703     771 
   Size:        703      68
 
BanI G'GyrC_C

Cuts at:      0     129     771 
   Size:        129     642
 
BanII G_rGCy'C

Cuts at:      0     206     710     771 
   Size:        206     504      61

  Fragments arranged by size: 

                 504     206      61
 
BbeI G_GCGC'C

Cuts at:      0     133     771 
   Size:        133     638
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     573     771 
   Size:        573     198
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     504     621     771 
   Size:        316     188     117     150

  Fragments arranged by size: 

                 316     188     150     117
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     358     771 
   Size:        358     413
 
BsaHI Gr'CG_yC

Cuts at:      0     130     771 
   Size:        130     641
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     314     344     771 
   Size:        314      30     427

  Fragments arranged by size: 

                 427     314      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94      97     335     663     739     771 
   Size:         94       3     238     328      76      32

  Fragments arranged by size: 

                 328     238      94      76      32       3
 
BseYI C'CCAG_C

Cuts at:      0     133     315     771 
   Size:        133     182     456

  Fragments arranged by size: 

                 456     182     133
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     771 
   Size:         76     695
 
BsiEI CG_ry'CG

Cuts at:      0     287     419     771 
   Size:        287     132     352

  Fragments arranged by size: 

                 352     287     132
 
BsiHKAI G_wGCw'C

Cuts at:      0     719     771 
   Size:        719      52
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     438     771 
   Size:        438     333
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     710     719     771 
   Size:        206     504       9      52

  Fragments arranged by size: 

                 504     206      52       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     539     771 
   Size:        539     232
 
BsrBI CCG'CTC

Cuts at:      0     197     407     771 
   Size:        197     210     364

  Fragments arranged by size: 

                 364     210     197
 
BssHII G'CGCG_C

Cuts at:      0     463     771 
   Size:        463     308
 
BstEII G'GTnAC_C

Cuts at:      0     303     771 
   Size:        303     468
 
BstYI r'GATC_y

Cuts at:      0     537     771 
   Size:        537     234
 
BtgI C'CryG_G

Cuts at:      0     281     495     771 
   Size:        281     214     276

  Fragments arranged by size: 

                 281     276     214
 
EaeI y'GGCC_r

Cuts at:      0     284     416     498     771 
   Size:        284     132      82     273

  Fragments arranged by size: 

                 284     273     132      82
 
EagI C'GGCC_G

Cuts at:      0     284     416     771 
   Size:        284     132     355

  Fragments arranged by size: 

                 355     284     132
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     504     621     771 
   Size:        316     188     117     150

  Fragments arranged by size: 

                 316     188     150     117
 
EcoRV GAT'ATC

Cuts at:      0     144     771 
   Size:        144     627
 
HaeII r_GCGC'y

Cuts at:      0     133     548     644     771 
   Size:        133     415      96     127

  Fragments arranged by size: 

                 415     133     127      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     592     624     771 
   Size:         34      32     526      32     147

  Fragments arranged by size: 

                 526     147      34      32      32
 
KasI G'GCGC_C

Cuts at:      0     129     771 
   Size:        129     642
 
MspA1I CmG'CkG

Cuts at:      0     294     476     497     771 
   Size:        294     182      21     274

  Fragments arranged by size: 

                 294     274     182      21
 
NarI GG'CG_CC

Cuts at:      0     130     771 
   Size:        130     641
 
PfoI T'CCnGG_A

Cuts at:      0     389     771 
   Size:        389     382
 
PstI C_TGCA'G

Cuts at:      0     122     536     771 
   Size:        122     414     235

  Fragments arranged by size: 

                 414     235     122
 
PvuII CAG'CTG

Cuts at:      0     294     771 
   Size:        294     477
 
SacII CC_GC'GG

Cuts at:      0     498     771 
   Size:        498     273
 
SbfI CC_TGCA'GG

Cuts at:      0     536     771 
   Size:        536     235
 
SfcI C'TryA_G

Cuts at:      0     118     532     771 
   Size:        118     414     239

  Fragments arranged by size: 

                 414     239     118
 
SfoI GGC'GCC

Cuts at:      0     131     771 
   Size:        131     640
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     322     771 
   Size:        322     449
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     314     346     385     417     771 
   Size:        314      32      39      32     354

  Fragments arranged by size: 

                 354     314      39      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     681     771 
   Size:        681      90
 
XmnI GAAnn'nnTTC

Cuts at:      0     154     771 
   Size:        154     617
 

 Enzymes that do cut:

     AclI    AlwNI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
     BpmI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI   BssHII   BstEII    BstYI
     BtgI     EaeI     EagI  Eco57MI    EcoRV    HaeII    Hin4I     KasI
   MspA1I     NarI     PfoI     PstI    PvuII    SacII     SbfI     SfcI
     SfoI    TaqII     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsiWI
     BsmI    BspEI    BspHI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     XbaI     XcmI     XhoI     XmaI     ZraI
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