Restriction map for pF1KB0430
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
AspEI       BalI        BamHI       BbeI        BbrPI       
BcgI        BclI        BfrI        BglII       BsaAI       
BsaBI       BsaI        BseAI       BsiEI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        KasI        MamI        MluI        MluNI       
MroI        MscI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SexAI       SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        TfiI        XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I       2217  GGTACC               G^GTACC
AccI         1064  GTCTAC               GT^[AC][GT]AC
AcyI          858  GACGCC               G[GA]^CG[CT]C
AflIII       2255  ACATGT               A^C[GA][CT]GT
Alw44I       2371  GTGCAC               G^TGCAC
ApaLI        2371  GTGCAC               G^TGCAC
Asp700I       318  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2217  GGTACC               G^GTACC
AviII         465  TGCGCA               TGC^GCA
AvrII        1498  CCTAGG               C^CTAGG
BfaI         1499  CTAG                 C^TAG
BlnI         1498  CCTAGG               C^CTAGG
BlpI         1603  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1603  GCTAAGC              GC^T[ACGT]AGC
BsaHI         858  GACGCC               G[GA]^CG[CT]C
BsiWI        2281  CGTACG               C^GTACG
BspLU11I     2255  ACATGT               A^CATGT
BssHII          8  GCGCGC               G^CGCGC
BssSI        2023  CTCGTG               CACGAG(-5/-1)
BstAPI       2196  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1044  CCACCTCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         356  AGATCC               [GA]^GATC[CT]
CelII        1603  GCTAAGC              GC^T[ACGT]AGC
DraIII       2033  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1055  GACACAGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1324  CCTGCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1687  GAATTC               G^AATTC
FspI          465  TGCGCA               TGC^GCA
Hin1I         858  GACGCC               G[GA]^CG[CT]C
HincII        979  GTTGAC               GT[CT]^[GA]AC
HindII        979  GTTGAC               GT[CT]^[GA]AC
KpnI         2217  GGTACC               GGTAC^C
MaeI         1499  CTAG                 C^TAG
MfeI          735  CAATTG               C^AATTG
MflI          356  AGATCC               [GA]^GATC[CT]
MseI         2183  TTAA                 T^TAA
MunI          735  CAATTG               C^AATTG
NaeI            4  GCCGGC               GCC^GGC
NcoI         1250  CCATGG               C^CATGG
NgoMIV          4  GCCGGC               G^CCGGC
NspI         2255  ACATGT               [GA]CATG^[CT]
PflMI        1861  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI          91  GACCCGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2167  GAGCTC               GAGCT^C
SbfI          444  CCTGCAGG             CCTGCA^GG
ScaI         2411  AGTACT               AGT^ACT
SfiI         1752  GGCCACATTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         2493  CCCGGG               CCC^GGG
Sse8387I      444  CCTGCAGG             CCTGCA^GG
Tru9I        2183  TTAA                 T^TAA
Van91I       1861  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         356  AGATCC               [GA]^GATC[CT]
XmaCI        2493  CCCGGG               C^CCGGG
XmaI         2493  CCCGGG               C^CCGGG
XmnI          318  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI          321  GAATTT               [GA]^AATT[CT]
             1687  GAATTC              
AlwI          357  GATCC                GGATC(4/5)
              843  GATCC               
AocI          454  CCTGAGG              CC^T[ACGT]AGG
              811  CCTGAGG             
ApaI          217  GGGCCC               GGGCC^C
             1008  GGGCCC              
ApoI          321  GAATTT               [GA]^AATT[CT]
             1687  GAATTC              
AspI          777  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2266  GACTACGTC           
AvaI           93  CCCGAG               C^[CT]CG[GA]G
             2493  CCCGGG              
BbsI         1138  GAAGAC               GAAGAC(2/6)
             2082  GAAGAC              
BbvCI        1848  CCTCAGC              CCTCAGC(-5/-2)
             1908  CCTCAGC             
BciVI        1636  GTATCC               GTATCC(6/5)
             2480  GTATCC              
BpuAI        1138  GAAGAC               GAAGAC(2/6)
             2082  GAAGAC              
BsaWI         470  ACCGGA               [AT]^CCGG[AT]
             1652  TCCGGT              
BsgI         1695  CTGCAC               GTGCAG(16/14)
             2287  CTGCAC              
BsoBI          93  CCCGAG               C^[CT]CG[GA]G
             2493  CCCGGG              
BsrDI         645  GCAATG               GCAATG(2/0)
              830  GCAATG              
BsrFI           4  GCCGGC               [GA]^CCGG[CT]
             1946  ACCGGC              
Bsu36I        454  CCTGAGG              CC^T[ACGT]AGG
              811  CCTGAGG             
Cfr10I          4  GCCGGC               [GA]^CCGG[CT]
             1946  ACCGGC              
DpnII         357  GATC                 ^GATC
              843  GATC                
Eco81I        454  CCTGAGG              CC^T[ACGT]AGG
              811  CCTGAGG             
HgaI          858  GACGC                GACGC(5/10)
             1880  GCGTC               
MaeII         824  ACGT                 A^CGT
             2270  ACGT                
MboI          357  GATC                 ^GATC
              843  GATC                
MslI          519  CATCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2097  CATCCTTATG          
NdeII         357  GATC                 ^GATC
              843  GATC                
PflFI         777  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2266  GACTACGTC           
Sau3AI        357  GATC                 ^GATC
              843  GATC                
StuI          330  AGGCCT               AGG^CCT
             1215  AGGCCT              
Tth111I       777  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2266  GACTACGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BanI        BanII       BbvI        
BcnI        BglI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeIII      
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
SacII       SapI        Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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