Restriction map for pF1KB0430
 (Linear) (Six-base) MAPSORT of: KIBB0430.seq  Check: 3958  from: 1  to: 2556



KIBB0430.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     617    2556 
   Size:        617    1939
 
AccI GT'mk_AC

Cuts at:      0    1065    2556 
   Size:       1065    1491
 
Acc65I G'GTAC_C

Cuts at:      0    2217    2556 
   Size:       2217     339
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     951     975    1157    2556 
   Size:        951      24     182    1399

  Fragments arranged by size: 

                1399     951     182      24
 
AflIII A'CryG_T

Cuts at:      0    2255    2556 
   Size:       2255     301
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2135    2556 
   Size:       2135     421
 
AlwNI CAG_nnn'CTG

Cuts at:      0     726    1684    1867    2148    2202    2556 
   Size:        726     958     183     281      54     354

  Fragments arranged by size: 

                 958     726     354     281     183      54
 
ApaI G_GGCC'C

Cuts at:      0     221    1012    2556 
   Size:        221     791    1544

  Fragments arranged by size: 

                1544     791     221
 
ApaLI G'TGCA_C

Cuts at:      0    2371    2556 
   Size:       2371     185
 
ApoI r'AATT_y

Cuts at:      0     321    1687    2556 
   Size:        321    1366     869

  Fragments arranged by size: 

                1366     869     321
 
AvaI C'yCGr_G

Cuts at:      0      93    2493    2556 
   Size:         93    2400      63

  Fragments arranged by size: 

                2400      93      63
 
AvrII C'CTAG_G

Cuts at:      0    1498    2556 
   Size:       1498    1058
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1935    1968    2201    2234    2556 
   Size:       1935      33     233      33     322

  Fragments arranged by size: 

                1935     322     233      33      33
 
BanI G'GyrC_C

Cuts at:      0    1017    1209    1254    2217    2536    2556 
   Size:       1017     192      45     963     319      20

  Fragments arranged by size: 

                1017     963     319     192      45      20
 
BanII G_rGCy'C

Cuts at:      0     221     421     959    1012    1226    2024    2171    2556 
   Size:        221     200     538      53     214     798     147     385

  Fragments arranged by size: 

                 798     538     385     221     214     200     147      53
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1145    2089    2556 
   Size:       1145     944     467

  Fragments arranged by size: 

                1145     944     467
 
BbvCI CC'TCA_GC

Cuts at:      0    1849    1909    2556 
   Size:       1849      60     647

  Fragments arranged by size: 

                1849     647      60
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1647    2491    2556 
   Size:       1647     844      65

  Fragments arranged by size: 

                1647     844      65
 
BglI GCCn_nnn'nGGC

Cuts at:      0     192     714    1759    2465    2521    2556 
   Size:        192     522    1045     706      56      35

  Fragments arranged by size: 

                1045     706     522     192      56      35
 
BlpI GC'TnA_GC

Cuts at:      0    1604    2556 
   Size:       1604     952
 
Bme1580I G_kGCm'C

Cuts at:      0     221    1012    1022    2043    2375    2470    2539    2556 
   Size:        221     791      10    1021     332      95      69      17

  Fragments arranged by size: 

                1021     791     332     221      95      69      17      10
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1818    2556 
   Size:       1818     738
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     399     514     651    1086    1240    1642    1727    2556 
   Size:        399     115     137     435     154     402      85     829

  Fragments arranged by size: 

                 829     435     402     399     154     137     115      85
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     611    1527    1849    1909    2507    2556 
   Size:        611     916     322      60     598      49

  Fragments arranged by size: 

                 916     611     598     322      60      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     319    2038    2556 
   Size:        297      22    1719     518

  Fragments arranged by size: 

                1719     518     297      22
 
BsaHI Gr'CG_yC

Cuts at:      0     859    2556 
   Size:        859    1697
 
BsaWI w'CCGG_w

Cuts at:      0     470    1652    2556 
   Size:        470    1182     904

  Fragments arranged by size: 

                1182     904     470
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     634     664    1305    1335    1837    1867    2556 
   Size:        634      30     641      30     502      30     689

  Fragments arranged by size: 

                 689     641     634     502      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     715     718    1639    2149    2179    2556 
   Size:        715       3     921     510      30     377

  Fragments arranged by size: 

                 921     715     510     377      30       3
 
BseYI C'CCAG_C

Cuts at:      0      55     583     687     961    1387    1392    1809    1816 
   Size:         55     528     104     274     426       5     417       7

Cuts at:   1816    1998    2042    2047    2141    2463    2556 
   Size:        182      44       5      94     322      93

  Fragments arranged by size: 

                 528     426     417     322     274     182     104      94
                  93      55      44       7       5       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1680    2272    2556 
   Size:       1680     592     284

  Fragments arranged by size: 

                1680     592     284
 
BsiHKAI G_wGCw'C

Cuts at:      0    2032    2171    2375    2556 
   Size:       2032     139     204     181

  Fragments arranged by size: 

                2032     204     181     139
 
BsiWI C'GTAC_G

Cuts at:      0    2281    2556 
   Size:       2281     275
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     421     959    1012    1022    1226    2024    2032 
   Size:        221     200     538      53      10     204     798       8

Cuts at:   2032    2043    2171    2375    2470    2539    2556 
   Size:         11     128     204      95      69      17

  Fragments arranged by size: 

                 798     538     221     204     204     200     128      95
                  69      53      17      11      10       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       7      27     123     617    2556 
   Size:          7      20      96     494    1939

  Fragments arranged by size: 

                1939     494      96      20       7
 
BsrBI CCG'CTC

Cuts at:      0     128    1797    2428    2556 
   Size:        128    1669     631     128

  Fragments arranged by size: 

                1669     631     128     128
 
BsrDI GCAATG_nn'

Cuts at:      0     652     837    2556 
   Size:        652     185    1719

  Fragments arranged by size: 

                1719     652     185
 
BsrFI r'CCGG_y

Cuts at:      0       4    1946    2556 
   Size:          4    1942     610

  Fragments arranged by size: 

                1942     610       4
 
BssHII G'CGCG_C

Cuts at:      0       8    2556 
   Size:          8    2548
 
BssSI C'ACGA_G

Cuts at:      0    2023    2556 
   Size:       2023     533
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2202    2556 
   Size:       2202     354
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1051    2556 
   Size:       1051    1505
 
BstYI r'GATC_y

Cuts at:      0     356    2556 
   Size:        356    2200
 
Bsu36I CC'TnA_GG

Cuts at:      0     455     812    2556 
   Size:        455     357    1744

  Fragments arranged by size: 

                1744     455     357
 
BtgI C'CryG_G

Cuts at:      0      26      43     212     485     974    1250    1967    2360 
   Size:         26      17     169     273     489     276     717     393

Cuts at:   2360    2556 
   Size:        196

  Fragments arranged by size: 

                 717     489     393     276     273     196     169      26
                  17
 
BtsI GCAGTG_nn'

Cuts at:      0     862    1002    1262    1538    1556    1830    2556 
   Size:        862     140     260     276      18     274     726

  Fragments arranged by size: 

                 862     726     276     274     260     140      18
 
DraIII CAC_nnn'GTG

Cuts at:      0    2038    2556 
   Size:       2038     518
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1061    2556 
   Size:       1061    1495
 
EaeI y'GGCC_r

Cuts at:      0       2      23     631    1034    1735    1751    2434    2556 
   Size:          2      21     608     403     701      16     683     122

  Fragments arranged by size: 

                 701     683     608     403     122      21      16       2
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     271     409     667     772     994    1274    1294    2556 
   Size:        271     138     258     105     222     280      20    1262

  Fragments arranged by size: 

                1262     280     271     258     222     138     105      20
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     349     634    2371    2556 
   Size:        349     285    1737     185

  Fragments arranged by size: 

                1737     349     285     185
 
EcoICRI GAG'CTC

Cuts at:      0    2169    2556 
   Size:       2169     387
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     399     514     651     951     975    1086    1157    1240 
   Size:        399     115     137     300      24     111      71      83

Cuts at:   1240    1642    1727    2556 
   Size:        402      85     829

  Fragments arranged by size: 

                 829     402     399     300     137     115     111      85
                  83      71      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1328    2556 
   Size:       1328    1228
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39      90     217     218     383     451     693    1001 
   Size:         39      51     127       1     165      68     242     308

Cuts at:   1001    1664    1772    1845    1864    2556 
   Size:        663     108      73      19     692

  Fragments arranged by size: 

                 692     663     308     242     165     127     108      73
                  68      51      39      19       1
 
EcoRI G'AATT_C

Cuts at:      0    1687    2556 
   Size:       1687     869
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2001    2556 
   Size:       2001     555
 
FspI TGC'GCA

Cuts at:      0     467    2556 
   Size:        467    2089
 
FspAI rTGC'GCAy

Cuts at:      0     467    2556 
   Size:        467    2089
 
HaeII r_GCGC'y

Cuts at:      0    1109    1816    1986    2556 
   Size:       1109     707     170     570

  Fragments arranged by size: 

                1109     707     570     170
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      67      87      99     341     373    1837    1869 
   Size:         55      12      20      12     242      32    1464      32

Cuts at:   1869    2556 
   Size:        687

  Fragments arranged by size: 

                1464     687     242      55      32      32      20      12
                  12
 
HincII GTy'rAC

Cuts at:      0     981    2556 
   Size:        981    1575
 
KpnI G_GTAC'C

Cuts at:      0    2221    2556 
   Size:       2221     335
 
MfeI C'AATT_G

Cuts at:      0     735    2556 
   Size:        735    1821
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     773    2556 
   Size:        248     525    1783

  Fragments arranged by size: 

                1783     525     248
 
MslI CAynn'nnrTG

Cuts at:      0     523    2101    2556 
   Size:        523    1578     455

  Fragments arranged by size: 

                1578     523     455
 
MspA1I CmG'CkG

Cuts at:      0      28      45     146     804    1082    1344    1590    1820 
   Size:         28      17     101     658     278     262     246     230

Cuts at:   1820    1969    2089    2148    2202    2463    2556 
   Size:        149     120      59      54     261      93

  Fragments arranged by size: 

                 658     278     262     261     246     230     149     120
                 101      93      59      54      28      17
 
NaeI GCC'GGC

Cuts at:      0       6    2556 
   Size:          6    2550
 
NcoI C'CATG_G

Cuts at:      0    1250    2556 
   Size:       1250    1306
 
NgoMIV G'CCGG_C

Cuts at:      0       4    2556 
   Size:          4    2552
 
NspI r_CATG'y

Cuts at:      0    2259    2556 
   Size:       2259     297
 
PasI CC'CwG_GG

Cuts at:      0    1005    1660    1867    2556 
   Size:       1005     655     207     689

  Fragments arranged by size: 

                1005     689     655     207
 
PciI A'CATG_T

Cuts at:      0    2255    2556 
   Size:       2255     301
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1867    2556 
   Size:       1867     689
 
PfoI T'CCnGG_A

Cuts at:      0     491     725    2556 
   Size:        491     234    1831

  Fragments arranged by size: 

                1831     491     234
 
PpuMI rG'GwC_Cy

Cuts at:      0      39      90     693    1001    1664    1845    2556 
   Size:         39      51     603     308     663     181     711

  Fragments arranged by size: 

                 711     663     603     308     181      51      39
 
PshAI GACnn'nnGTC

Cuts at:      0      95    2556 
   Size:         95    2461
 
PspOMI G'GGCC_C

Cuts at:      0     217    1008    2556 
   Size:        217     791    1548

  Fragments arranged by size: 

                1548     791     217
 
PstI C_TGCA'G

Cuts at:      0     349     449     803     869    1277    1359    2081    2556 
   Size:        349     100     354      66     408      82     722     475

  Fragments arranged by size: 

                 722     475     408     354     349     100      82      66
            
 
PvuII CAG'CTG

Cuts at:      0     804    1082    1590    2089    2148    2202    2463    2556 
   Size:        804     278     508     499      59      54     261      93

  Fragments arranged by size: 

                 804     508     499     278     261      93      59      54
            
 
SacI G_AGCT'C

Cuts at:      0    2171    2556 
   Size:       2171     385
 
SacII CC_GC'GG

Cuts at:      0      29      46    1970    2556 
   Size:         29      17    1924     586

  Fragments arranged by size: 

                1924     586      29      17
 
SanDI GG'GwC_CC

Cuts at:      0     693    1001    1664    2556 
   Size:        693     308     663     892

  Fragments arranged by size: 

                 892     693     663     308
 
SapI GCTCTTCn'nnn_

Cuts at:      0     409     667    1274    2556 
   Size:        409     258     607    1282

  Fragments arranged by size: 

                1282     607     409     258
 
SbfI CC_TGCA'GG

Cuts at:      0     449    2556 
   Size:        449    2107
 
ScaI AGT'ACT

Cuts at:      0    2413    2556 
   Size:       2413     143
 
SfcI C'TryA_G

Cuts at:      0     288     345     445     799     865    1066    1273    1355 
   Size:        288      57     100     354      66     201     207      82

Cuts at:   1355    2077    2107    2556 
   Size:        722      30     449

  Fragments arranged by size: 

                 722     449     354     288     207     201     100      82
                  66      57      30
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1759    2556 
   Size:       1759     797
 
SmaI CCC'GGG

Cuts at:      0    2495    2556 
   Size:       2495      61
 
SmlI C'TyrA_G

Cuts at:      0     276     334    2053    2556 
   Size:        276      58    1719     503

  Fragments arranged by size: 

                1719     503     276      58
 
StuI AGG'CCT

Cuts at:      0     332    1217    2556 
   Size:        332     885    1339

  Fragments arranged by size: 

                1339     885     332
 
StyI C'CwwG_G

Cuts at:      0    1094    1205    1250    1427    1498    2541    2556 
   Size:       1094     111      45     177      71    1043      15

  Fragments arranged by size: 

                1094    1043     177     111      71      45      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1275    1383    1954    2295    2503    2556 
   Size:       1275     108     571     341     208      53

  Fragments arranged by size: 

                1275     571     341     208     108      53
 
TatI w'GTAC_w

Cuts at:      0    2093    2411    2556 
   Size:       2093     318     145

  Fragments arranged by size: 

                2093     318     145
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     613     634     645     666    1226    1258    1305    1337 
   Size:        613      21      11      21     560      32      47      32

Cuts at:   1337    1367    1399    1530    1562    1689    1721    2556 
   Size:         30      32     131      32     127      32     835

  Fragments arranged by size: 

                 835     613     560     131     127      47      32      32
                  32      32      32      30      21      21      11
 
Tth111I GACn'n_nGTC

Cuts at:      0     780    2269    2556 
   Size:        780    1489     287

  Fragments arranged by size: 

                1489     780     287
 
XmaI C'CCGG_G

Cuts at:      0    2493    2556 
   Size:       2493      63
 
XmnI GAAnn'nnTTC

Cuts at:      0     322    2556 
   Size:        322    2234
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    AvrII     BaeI     BanI    BanII     BbsI
    BbvCI    BciVI     BglI     BlpI Bme1580I     BplI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsiWI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KpnI     MfeI
     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI    SacII    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI
     AscI     AseI    AsiSI    BamHI     BbeI     BcgI     BclI    BfrBI
    BglII    BmgBI     BmrI     BmtI     BsaI    BsaAI    BsaBI    BsiEI
     BsmI    BsmBI    BspEI    BspHI    BsrGI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI     EagI    EcoRV     FseI  HindIII
     HpaI     KasI     MluI     MscI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SalI    SexAI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XcmI
     XhoI     ZraI
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