Restriction map for pF1KB6684
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AlwI        
AlwNI       AocI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BlnI        BsaAI       BsaBI       BsaHI       BsaI        
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstUI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      EaeI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoRV       FauI        
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Van91I      
XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AciI          955  CCGC                 CCGC(-3/-1)
AfaI          585  GTAC                 GT^AC
AflIII        690  ACATGT               A^C[GA][CT]GT
Alw44I        790  GTGCAC               G^TGCAC
Aor51HI       608  AGCGCT               AGC^GCT
ApaI          503  GGGCCC               GGGCC^C
ApaLI         790  GTGCAC               G^TGCAC
AspI          872  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          421  CTCGAG               C^[CT]CG[GA]G
BanI          136  GGCACC               G^G[CT][GA]CC
BanII         503  GGGCCC               G[GA]GC[CT]^C
BbsI         1206  GTCTTC               GAAGAC(2/6)
BclI          774  TGATCA               T^GATCA
BglII         425  AGATCT               A^GATCT
BlpI           54  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       54  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1206  GTCTTC               GAAGAC(2/6)
BsaWI        1232  ACCGGA               [AT]^CCGG[AT]
BseRI         160  CTCCTC               GAGGAG(10/8)
BsgI         1257  CTGCAC               GTGCAG(16/14)
BsmI          336  GAATGC               GAATGC(1/-1)
BsoBI         421  CTCGAG               C^[CT]CG[GA]G
BspLU11I      690  ACATGT               A^CATGT
BspMI         751  GCAGGT               ACCTGC(4/8)
BsrBI         955  CCGCTC               CCGCTC(-3/-3)
BstAPI        331  GCAACGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         174  TTCGAA               TT^CGAA
BstEII       1074  GGTTACC              G^GT[ACGT]ACC
BstPI        1074  GGTTACC              G^GT[ACGT]ACC
BstYI         425  AGATCT               [GA]^GATC[CT]
CelII          54  GCTCAGC              GC^T[ACGT]AGC
DrdI          697  GACTACTTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      608  AGCGCT               AGC^GCT
EcoNI         908  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1074  GGTTACC              G^GT[ACGT]ACC
EcoRI         816  GAATTC               G^AATTC
EcoT22I      1012  ATGCAT               ATGCA^T
FbaI          774  TGATCA               T^GATCA
HapII        1233  CCGG                 C^CGG
HgaI          872  GACGC                GACGC(5/10)
HincII        706  GTCAAC               GT[CT]^[GA]AC
HindII        706  GTCAAC               GT[CT]^[GA]AC
HpaII        1233  CCGG                 C^CGG
MaeII         509  ACGT                 A^CGT
MflI          425  AGATCT               [GA]^GATC[CT]
MspI         1233  CCGG                 C^CGG
NcoI          264  CCATGG               C^CATGG
NsiI         1012  ATGCAT               ATGCA^T
NspV          174  TTCGAA               TT^CGAA
PaeR7I        421  CTCGAG               C^TCGAG
PflFI         872  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          380  GAGTC                GAGTC(4/5)
PpuMI           9  AGGTCCC              [GA]G^G[AT]CC[CT]
RsaI          585  GTAC                 GT^AC
SfcI          374  CTACAG               C^T[GA][CT]AG
SfuI          174  TTCGAA               TT^CGAA
SmlI          421  CTCGAG               C^T[CT][GA]AG
SphI         1010  GCATGC               GCATG^C
Tth111I       872  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          717  CCAAGACCAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          421  CTCGAG               C^TCGAG
XhoII         425  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          816  GAATTC               [GA]^AATT[CT]
             1192  AAATTC              
ApoI          816  GAATTC               [GA]^AATT[CT]
             1192  AAATTC              
AspHI         790  GTGCAC               G[AT]GC[AT]^C
              998  GTGCTC              
BfaI          182  CTAG                 C^TAG
              196  CTAG                
BpmI          620  CTGGAG               CTGGAG(16/14)
              811  CTGGAG              
BsiHKAI       790  GTGCAC               G[AT]GC[AT]^C
              998  GTGCTC              
BssSI         588  CACGAG               CACGAG(-5/-1)
             1024  CTCGTG              
DsaI          264  CCATGG               C^C[GA][CT]GG
              353  CCGTGG              
HphI         1097  TCACC                GGTGA(8/7)
             1115  TCACC               
MaeI          182  CTAG                 C^TAG
              196  CTAG                
MseI          410  TTAA                 T^TAA
              890  TTAA                
MspA1I        392  CAGCTG               C[AC]G^C[GT]G
              544  CAGCTG              
NspI          690  ACATGT               [GA]CATG^[CT]
             1010  GCATGC              
PvuII         392  CAGCTG               CAG^CTG
              544  CAGCTG              
SapI          604  GAAGAGC              GCTCTTC(1/4)
              531  GCTCTTC             
StuI          561  AGGCCT               AGG^CCT
              857  AGGCCT              
Tru9I         410  TTAA                 T^TAA
              890  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AccI        AluI        AvaII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HhaI        
HinP1I      HinfI       ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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