Restriction map for pF1KB6684
 (Linear) (Six-base) MAPSORT of: KIBB6684.seq  Check: 7701  from: 1  to: 1368



KIBB6684.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:      0     373     383     977    1368 
   Size:        373      10     594     391

  Fragments arranged by size: 

                 594     391     373      10
 
AfeI AGC'GCT

Cuts at:      0     610    1368 
   Size:        610     758
 
AflIII A'CryG_T

Cuts at:      0     690    1368 
   Size:        690     678
 
ApaI G_GGCC'C

Cuts at:      0     507    1368 
   Size:        507     861
 
ApaLI G'TGCA_C

Cuts at:      0     790    1368 
   Size:        790     578
 
ApoI r'AATT_y

Cuts at:      0     816    1192    1368 
   Size:        816     376     176

  Fragments arranged by size: 

                 816     376     176
 
AvaI C'yCGr_G

Cuts at:      0     421    1368 
   Size:        421     947
 
BanI G'GyrC_C

Cuts at:      0     136    1368 
   Size:        136    1232
 
BanII G_rGCy'C

Cuts at:      0     507    1368 
   Size:        507     861
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1199    1368 
   Size:       1199     169
 
BclI T'GATC_A

Cuts at:      0     774    1368 
   Size:        774     594
 
BfrBI ATG'CAT

Cuts at:      0    1014    1368 
   Size:       1014     354
 
BglII A'GATC_T

Cuts at:      0     425    1368 
   Size:        425     943
 
BlpI GC'TnA_GC

Cuts at:      0      55    1368 
   Size:         55    1313
 
Bme1580I G_kGCm'C

Cuts at:      0     507     794    1368 
   Size:        507     287     574

  Fragments arranged by size: 

                 574     507     287
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     646    1368 
   Size:        646     722
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     832    1368 
   Size:        641     191     536

  Fragments arranged by size: 

                 641     536     191
 
Bpu10I CC'TnA_GC

Cuts at:      0    1084    1124    1368 
   Size:       1084      40     244

  Fragments arranged by size: 

                1084     244      40
 
BsaWI w'CCGG_w

Cuts at:      0    1232    1368 
   Size:       1232     136
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     151    1368 
   Size:        151    1217
 
BseYI C'CCAG_C

Cuts at:      0     456     546    1368 
   Size:        456      90     822

  Fragments arranged by size: 

                 822     456      90
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1242    1368 
   Size:       1242     126
 
BsiHKAI G_wGCw'C

Cuts at:      0     794    1002    1368 
   Size:        794     208     366

  Fragments arranged by size: 

                 794     366     208
 
BsmI GAATG_Cn'

Cuts at:      0     342    1368 
   Size:        342    1026
 
Bsp1286I G_dGCh'C

Cuts at:      0     507     794    1002    1368 
   Size:        507     287     208     366

  Fragments arranged by size: 

                 507     366     287     208
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     742    1368 
   Size:        742     626
 
BsrBI CCG'CTC

Cuts at:      0     957    1368 
   Size:        957     411
 
BssSI C'ACGA_G

Cuts at:      0     588    1024    1368 
   Size:        588     436     344

  Fragments arranged by size: 

                 588     436     344
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     337    1368 
   Size:        337    1031
 
BstBI TT'CG_AA

Cuts at:      0     175    1368 
   Size:        175    1193
 
BstEII G'GTnAC_C

Cuts at:      0    1074    1368 
   Size:       1074     294
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      14     226     487    1368 
   Size:         14     212     261     881

  Fragments arranged by size: 

                 881     261     212      14
 
BstYI r'GATC_y

Cuts at:      0     425    1368 
   Size:        425     943
 
BtgI C'CryG_G

Cuts at:      0     264     353    1368 
   Size:        264      89    1015

  Fragments arranged by size: 

                1015     264      89
 
BtsI GCAGTG_nn'

Cuts at:      0     216    1259    1368 
   Size:        216    1043     109

  Fragments arranged by size: 

                1043     216     109
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     703    1368 
   Size:        703     665
 
EarI CTCTTCn'nnn_

Cuts at:      0     538     599     637    1107    1368 
   Size:        538      61      38     470     261

  Fragments arranged by size: 

                 538     470     261      61      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     832    1368 
   Size:        641     191     536

  Fragments arranged by size: 

                 641     536     191
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     912    1368 
   Size:        912     456
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10      39     503    1368 
   Size:         10      29     464     865

  Fragments arranged by size: 

                 865     464      29      10
 
EcoRI G'AATT_C

Cuts at:      0     816    1368 
   Size:        816     552
 
HaeII r_GCGC'y

Cuts at:      0     122     612     809    1368 
   Size:        122     490     197     559

  Fragments arranged by size: 

                 559     490     197     122
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     759     791    1368 
   Size:        759      32     577

  Fragments arranged by size: 

                 759     577      32
 
HincII GTy'rAC

Cuts at:      0     708    1368 
   Size:        708     660
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     446     666    1235    1368 
   Size:         42     404     220     569     133

  Fragments arranged by size: 

                 569     404     220     133      42
 
MspA1I CmG'CkG

Cuts at:      0     394     546    1368 
   Size:        394     152     822

  Fragments arranged by size: 

                 822     394     152
 
NcoI C'CATG_G

Cuts at:      0     264    1368 
   Size:        264    1104
 
NsiI A_TGCA'T

Cuts at:      0    1016    1368 
   Size:       1016     352
 
NspI r_CATG'y

Cuts at:      0     694    1014    1368 
   Size:        694     320     354

  Fragments arranged by size: 

                 694     354     320
 
PasI CC'CwG_GG

Cuts at:      0      14    1368 
   Size:         14    1354
 
PciI A'CATG_T

Cuts at:      0     690    1368 
   Size:        690     678
 
PfoI T'CCnGG_A

Cuts at:      0     295    1368 
   Size:        295    1073
 
PpuMI rG'GwC_Cy

Cuts at:      0      10    1368 
   Size:         10    1358
 
PspOMI G'GGCC_C

Cuts at:      0     503    1368 
   Size:        503     865
 
PvuII CAG'CTG

Cuts at:      0     394     546    1368 
   Size:        394     152     822

  Fragments arranged by size: 

                 822     394     152
 
SapI GCTCTTCn'nnn_

Cuts at:      0     538     599    1368 
   Size:        538      61     769

  Fragments arranged by size: 

                 769     538      61
 
SfcI C'TryA_G

Cuts at:      0     374    1368 
   Size:        374     994
 
SmlI C'TyrA_G

Cuts at:      0     421    1368 
   Size:        421     947
 
SphI G_CATG'C

Cuts at:      0    1014    1368 
   Size:       1014     354
 
StuI AGG'CCT

Cuts at:      0     563     859    1368 
   Size:        563     296     509

  Fragments arranged by size: 

                 563     509     296
 
StyI C'CwwG_G

Cuts at:      0     264     913     962    1368 
   Size:        264     649      49     406

  Fragments arranged by size: 

                 649     406     264      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21     448    1368 
   Size:         21     427     920

  Fragments arranged by size: 

                 920     427      21
 
TatI w'GTAC_w

Cuts at:      0     584    1368 
   Size:        584     784
 
Tth111I GACn'n_nGTC

Cuts at:      0     875    1368 
   Size:        875     493
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     724    1368 
   Size:        724     644
 
XhoI C'TCGA_G

Cuts at:      0     421    1368 
   Size:        421     947
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbsI     BclI    BfrBI    BglII     BlpI Bme1580I     BplI
     BpmI   Bpu10I    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrBI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI     BtsI     DrdI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    HaeII    Hin4I   HincII     MmeI   MspA1I     NcoI     NsiI
     NspI     PasI     PciI     PfoI    PpuMI   PspOMI    PvuII     SapI
     SfcI     SmlI     SphI     StuI     StyI    TaqII     TatI  Tth111I
     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BglI    BmgBI
     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI     TstI     XbaI     XmaI
     XmnI     ZraI
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