Restriction map for pF1KB6566
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspHI       AvrII       BamHI       
BbeI        BbrPI       BfrI        BglII       BlnI        
BsaAI       BsaI        BseAI       BsgI        BsiHKAI     
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        HincII      HindII      HindIII     HpaI        
KasI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NruI        NsiI        NspV        
PacI        PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Van91I      XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        344  GGTACC               G^GTACC
AcyI          199  GGCGTC               G[GA]^CG[CT]C
AhdI         1102  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          364  GGGCCC               GGGCC^C
Asp718I       344  GGTACC               G^GTACC
AspEI        1102  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          207  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          961  GAAGAC               GAAGAC(2/6)
BbvCI         672  GCTGAGG              CCTCAGC(-5/-2)
BciVI         133  GTATCC               GTATCC(6/5)
BclI         1022  TGATCA               T^GATCA
BfaI          322  CTAG                 C^TAG
BlpI          939  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      939  GCTGAGC              GC^T[ACGT]AGC
BpuAI         961  GAAGAC               GAAGAC(2/6)
BsaBI         603  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         199  GGCGTC               G[GA]^CG[CT]C
BsaWI         788  ACCGGA               [AT]^CCGG[AT]
BsmAI         508  GAGAC                GTCTC(1/5)
BsmBI         508  GAGACG               CGTCTC(1/5)
BsmI          104  GCATTC               GAATGC(1/-1)
CelII         939  GCTGAGC              GC^T[ACGT]AGC
DsaI          126  CCGCGG               C^C[GA][CT]GG
EagI          123  CGGCCG               C^GGCCG
Eam1105I     1102  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          625  GAAGAG               CTCTTC(1/4)
EclXI         123  CGGCCG               C^GGCCG
Eco52I        123  CGGCCG               C^GGCCG
FbaI         1022  TGATCA               T^GATCA
HaeII        1017  GGCGCT               [GA]GCGC^[CT]
Hin1I         199  GGCGTC               G[GA]^CG[CT]C
KpnI          344  GGTACC               GGTAC^C
Ksp632I       625  GAAGAG               CTCTTC(1/4)
KspI          126  CCGCGG               CCGC^GG
MaeI          322  CTAG                 C^TAG
MaeII          34  ACGT                 A^CGT
MamI          603  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          528  CATGAGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI          122  GCGGCCGC             GC^GGCCGC
NspI          752  ACATGC               [GA]CATG^[CT]
PaeR7I       1150  CTCGAG               C^TCGAG
PflFI         207  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII         126  CCGCGG               CCGC^GG
SapI          625  GAAGAGC              GCTCTTC(1/4)
Tth111I       207  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          587  CCAGGACCGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1150  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwI          333  GATCC                GGATC(4/5)
              741  GATCC               
AvaI          171  CTCGGG               C^[CT]CG[GA]G
             1150  CTCGAG              
AviII         524  TGCGCA               TGC^GCA
              557  TGCGCA              
BalI          584  TGGCCA               TGG^CCA
              849  TGGCCA              
BcgI          725  GCACCGATCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1142  GCAGCCTGCTCG        
BseRI         168  CTCCTC               GAGGAG(10/8)
              655  GAGGAG              
BsiEI         123  CGGCCG               CG[GA][CT]^CG
              264  CGACCG              
BsmFI         413  GGGAC                GGGAC(10/14)
             1155  GGGAC               
BsoBI         171  CTCGGG               C^[CT]CG[GA]G
             1150  CTCGAG              
BsrBI         716  CCGCTC               CCGCTC(-3/-3)
             1069  GAGCGG              
BstYI         332  AGATCC               [GA]^GATC[CT]
              740  AGATCC              
EcoNI         282  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1146  CCTGCTCGAGG         
EcoT14I       956  CCTTGG               C^C[AT][AT]GG
             1190  CCAAGG              
FspI          524  TGCGCA               TGC^GCA
              557  TGCGCA              
HgaI          200  GCGTC                GACGC(5/10)
              211  GCGTC               
HphI          327  GGTGA                GGTGA(8/7)
              812  TCACC               
MflI          332  AGATCC               [GA]^GATC[CT]
              740  AGATCC              
MluNI         584  TGGCCA               TGG^CCA
              849  TGGCCA              
MscI          584  TGGCCA               TGG^CCA
              849  TGGCCA              
PpuMI         404  AGGACCT              [GA]G^G[AT]CC[CT]
             1193  AGGTCCT             
SfaNI         603  GATGC                GCATC(5/9)
              796  GATGC               
SmlI          916  CTTGAG               C^T[CT][GA]AG
             1150  CTCGAG              
StuI           48  AGGCCT               AGG^CCT
              615  AGGCCT              
StyI          956  CCTTGG               C^C[AT][AT]GG
             1190  CCAAGG              
Tsp45I        749  GTGAC                ^GT[GC]AC
              933  GTCAC               
Tsp509I       830  AATT                 ^AATT
             1177  AATT                
TspRI         699  CAGTG                CA[GC]TG[ACGT][ACGT]^
              968  CACTG               
XhoII         332  AGATCC               [GA]^GATC[CT]
              740  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AvaII       BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaJI       BsiYI       
BslI        Bsp1286I    BspMI       BsrI        BssHII      
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        TaqI        TfiI        
TseI        TthHB8I     
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