Restriction map for pF1KB6553
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HincII      HindII      HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NdeI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        Tru9I       Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII         882  CGCG                 CG^CG
AfaI          441  GTAC                 GT^AC
AlwNI         788  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         676  GTGCTC               G[AT]GC[AT]^C
BbrPI         781  CACGTG               CAC^GTG
BbsI          226  GTCTTC               GAAGAC(2/6)
BpuAI         226  GTCTTC               GAAGAC(2/6)
BsaAI         781  CACGTG               [CT]AC^GT[GA]
BsiHKAI       676  GTGCTC               G[AT]GC[AT]^C
BsrDI         161  GCAATG               GCAATG(2/0)
BstUI         882  CGCG                 CG^CG
BstYI         968  GGATCT               [GA]^GATC[CT]
EagI          472  CGGCCG               C^GGCCG
EarI          523  CTCTTC               CTCTTC(1/4)
EclXI         472  CGGCCG               C^GGCCG
Eco52I        472  CGGCCG               C^GGCCG
EcoT22I       974  ATGCAT               ATGCA^T
HindIII      1011  AAGCTT               A^AGCTT
Ksp632I       523  CTCTTC               CTCTTC(1/4)
MflI          968  GGATCT               [GA]^GATC[CT]
MslI          448  CATGGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          882  CGCG                 CG^CG
NaeI          197  GCCGGC               GCC^GGC
NcoI          447  CCATGG               C^CATGG
NgoMIV        197  GCCGGC               G^CCGGC
NheI          105  GCTAGC               G^CTAGC
NsiI          974  ATGCAT               ATGCA^T
NspI          365  GCATGT               [GA]CATG^[CT]
PflMI         959  CCAACCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1066  GAGTC                GAGTC(4/5)
PmaCI         781  CACGTG               CAC^GTG
PmlI          781  CACGTG               CAC^GTG
PstI          352  CTGCAG               CTGCA^G
RsaI          441  GTAC                 GT^AC
SapI          522  GCTCTTC              GCTCTTC(1/4)
SfcI          352  CTGCAG               C^T[GA][CT]AG
StuI          727  AGGCCT               AGG^CCT
TfiI          693  GATTC                G^A[AT]TC
Van91I        959  CCAACCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         968  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII        222  ACACGT               A^C[GA][CT]GT
              782  ACGTGT              
AlwI          387  GATCC                GGATC(4/5)
              968  GGATC               
BbeI          737  GGCGCC               GGCGC^C
              912  GGCGCC              
BseRI         375  CTCCTC               GAGGAG(10/8)
             1086  CTCCTC              
BsiEI         306  CGACCG               CG[GA][CT]^CG
              472  CGGCCG              
BstF5I         37  CATCC                GGATG(2/0)
              812  GGATG               
BstXI         395  CCATGACGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              447  CCATGGAAGTGG        
DpnII         387  GATC                 ^GATC
              969  GATC                
EcoNI         901  CCTCTGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1092  CCTGGCCAAGG         
EcoT14I       447  CCATGG               C^C[AT][AT]GG
             1097  CCAAGG              
FokI           37  CATCC                GGATG(9/13)
              812  GGATG               
HinfI         693  GATTC                G^A[ACGT]TC
             1066  GAGTC               
HphI          769  GGTGA                GGTGA(8/7)
              617  TCACC               
KasI          737  GGCGCC               G^GCGCC
              912  GGCGCC              
MaeIII        565  GTCAC                ^GT[ACGT]AC
              616  GTCAC               
MboI          387  GATC                 ^GATC
              969  GATC                
NarI          737  GGCGCC               GG^CGCC
              912  GGCGCC              
NdeII         387  GATC                 ^GATC
              969  GATC                
Sau3AI        387  GATC                 ^GATC
              969  GATC                
SfoI          737  GGCGCC               GGC^GCC
              912  GGCGCC              
StyI          447  CCATGG               C^C[AT][AT]GG
             1097  CCAAGG              
Tsp45I        565  GTCAC                ^GT[GC]AC
              616  GTCAC               
XcmI          134  CCATAGTCTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1082  CCAGCTCCTCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcyI        AluI        AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BsaHI       BsaJI       
BsgI        BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BstNI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
ItaI        MaeI        MaeII       MboII       MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PpuMI       
PvuII       Sau96I      ScrFI       SfaNI       SfiI        
SmaI        TseI        TspRI       XmaCI       XmaI        
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