Restriction map for pF1KB6647
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvrII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstUI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MluI        MroI        MseI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StyI        SwaI        Tru9I       XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          349  GTCTAC               GT^[AC][GT]AC
AfaI         1199  GTAC                 GT^AC
AflIII        427  ACATGT               A^C[GA][CT]GT
AspHI          65  GTGCTC               G[AT]GC[AT]^C
AspI          324  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          590  CCCGGG               C^[CT]CG[GA]G
AviII         659  TGCGCA               TGC^GCA
BalI          905  TGGCCA               TGG^CCA
BbvI            4  GCTGC                GCAGC(8/12)
BfaI         1021  CTAG                 C^TAG
BsaI          322  GAGACC               GGTCTC(1/5)
BsaWI         290  TCCGGT               [AT]^CCGG[AT]
BsgI         1154  GTGCAG               GTGCAG(16/14)
BsiHKAI        65  GTGCTC               G[AT]GC[AT]^C
BsoBI         590  CCCGGG               C^[CT]CG[GA]G
BspLU11I      427  ACATGT               A^CATGT
BsrDI         301  GCAATG               GCAATG(2/0)
BssSI         227  CTCGTG               CACGAG(-5/-1)
BstEII        579  GGTGACC              G^GT[ACGT]ACC
BstPI         579  GGTGACC              G^GT[ACGT]ACC
CfoI          660  GCGC                 GCG^C
DrdI          426  GACATGTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          921  CCACGG               C^C[GA][CT]GG
EaeI          905  TGGCCA               [CT]^GGCC[GA]
EcoO65I       579  GGTGACC              G^GT[ACGT]ACC
FspI          659  TGCGCA               TGC^GCA
HhaI          660  GCGC                 GCG^C
HinP1I        660  GCGC                 G^CGC
HindIII       176  AAGCTT               A^AGCTT
HphI          579  GGTGA                GGTGA(8/7)
MaeI         1021  CTAG                 C^TAG
MfeI          715  CAATTG               C^AATTG
MluNI         905  TGGCCA               TGG^CCA
MscI          905  TGGCCA               TGG^CCA
MslI          472  CACTTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          715  CAATTG               C^AATTG
NlaIII        428  CATG                 CATG^
NspI          427  ACATGT               [GA]CATG^[CT]
PflFI         324  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         185  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         897  CAGCTG               CAG^CTG
RsaI         1199  GTAC                 GT^AC
SapI          552  GCTCTTC              GCTCTTC(1/4)
SbfI          451  CCTGCAGG             CCTGCA^GG
ScaI         1198  AGTACT               AGT^ACT
SmaI          590  CCCGGG               CCC^GGG
Sse8387I      451  CCTGCAGG             CCTGCA^GG
TaqI          962  TCGA                 T^CGA
TseI            4  GCTGC                G^C[AT]GC
Tsp45I        580  GTGAC                ^GT[GC]AC
Tth111I       324  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       962  TCGA                 T^CGA
XmaCI         590  CCCGGG               C^CCGGG
XmaI          590  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwNI         139  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              989  CAGGCTCTG           
AocI          877  CCTCAGG              CC^T[ACGT]AGG
             1180  CCTGAGG             
ApaI          119  GGGCCC               GGGCC^C
              925  GGGCCC              
BamHI         269  GGATCC               G^GATCC
             1037  GGATCC              
BanI          293  GGTGCC               G^G[CT][GA]CC
              492  GGTGCC              
BpmI          703  CTGGAG               CTGGAG(16/14)
             1202  CTGGAG              
BstXI         354  CCAGATTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              413  CCAATAAGGTGG        
Bsu36I        877  CCTCAGG              CC^T[ACGT]AGG
             1180  CCTGAGG             
Eco81I        877  CCTCAGG              CC^T[ACGT]AGG
             1180  CCTGAGG             
MaeIII        580  GTGAC                ^GT[ACGT]AC
              763  GTAAC               
MspA1I        139  CAGCGG               C[AC]G^C[GT]G
              897  CAGCTG              
PleI          459  GACTC                GAGTC(4/5)
              341  GAGTC               
SfaNI         950  GCATC                GCATC(5/9)
              956  GCATC               
StuI          636  AGGCCT               AGG^CCT
             1047  AGGCCT              
Tsp509I       375  AATT                 ^AATT
              716  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanII       
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoNI       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MboI        MboII       MflI        MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIV       PflMI       PstI        Sau3AI      Sau96I      
ScrFI       SfcI        TfiI        TspRI       Van91I      
XhoII       
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