Restriction map for pF1KB6647
 (Linear) (Six-base) MAPSORT of: KIBB6647.seq  Check: 5157  from: 1  to: 1212



KIBB6647.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:      0     350    1212 
   Size:        350     862
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     612    1212 
   Size:        127     485     600

  Fragments arranged by size: 

                 600     485     127
 
AflIII A'CryG_T

Cuts at:      0     427    1212 
   Size:        427     785
 
AlwNI CAG_nnn'CTG

Cuts at:      0     144     994    1212 
   Size:        144     850     218

  Fragments arranged by size: 

                 850     218     144
 
ApaI G_GGCC'C

Cuts at:      0     123     929    1212 
   Size:        123     806     283

  Fragments arranged by size: 

                 806     283     123
 
AvaI C'yCGr_G

Cuts at:      0     590    1212 
   Size:        590     622
 
BamHI G'GATC_C

Cuts at:      0     269    1037    1212 
   Size:        269     768     175

  Fragments arranged by size: 

                 768     269     175
 
BanI G'GyrC_C

Cuts at:      0     293     492    1212 
   Size:        293     199     720

  Fragments arranged by size: 

                 720     293     199
 
BanII G_rGCy'C

Cuts at:      0     123     554     929    1212 
   Size:        123     431     375     283

  Fragments arranged by size: 

                 431     375     283     123
 
Bme1580I G_kGCm'C

Cuts at:      0     123     830     929    1212 
   Size:        123     707      99     283

  Fragments arranged by size: 

                 707     283     123      99
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1212 
   Size:        724     488
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     316    1212 
   Size:        316     896
 
BsaWI w'CCGG_w

Cuts at:      0     290    1212 
   Size:        290     922
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      14      44     313     343     500     530    1212 
   Size:         14      30     269      30     157      30     682

  Fragments arranged by size: 

                 682     269     157      30      30      30      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     218     611     721     788     988    1121    1126    1159 
   Size:        218     393     110      67     200     133       5      33

Cuts at:   1159    1162    1212 
   Size:          3      50

  Fragments arranged by size: 

                 393     218     200     133     110      67      50      33
                   5       3
 
BseYI C'CCAG_C

Cuts at:      0     137     585     788     844    1011    1212 
   Size:        137     448     203      56     167     201

  Fragments arranged by size: 

                 448     203     201     167     137      56
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1175    1212 
   Size:       1175      37
 
BsiHKAI G_wGCw'C

Cuts at:      0      69    1212 
   Size:         69    1143
 
Bsp1286I G_dGCh'C

Cuts at:      0      69     123     554     830     929    1212 
   Size:         69      54     431     276      99     283

  Fragments arranged by size: 

                 431     283     276      99      69      54
 
BsrDI GCAATG_nn'

Cuts at:      0     308    1212 
   Size:        308     904
 
BssSI C'ACGA_G

Cuts at:      0     227    1212 
   Size:        227     985
 
BstEII G'GTnAC_C

Cuts at:      0     579    1212 
   Size:        579     633
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     361     420    1212 
   Size:        361      59     792

  Fragments arranged by size: 

                 792     361      59
 
BstYI r'GATC_y

Cuts at:      0     269     600    1037    1212 
   Size:        269     331     437     175

  Fragments arranged by size: 

                 437     331     269     175
 
Bsu36I CC'TnA_GG

Cuts at:      0     878    1181    1212 
   Size:        878     303      31

  Fragments arranged by size: 

                 878     303      31
 
BtgI C'CryG_G

Cuts at:      0     921    1212 
   Size:        921     291
 
BtsI GCAGTG_nn'

Cuts at:      0     559    1212 
   Size:        559     653
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     432    1212 
   Size:        432     780
 
EaeI y'GGCC_r

Cuts at:      0     905    1212 
   Size:        905     307
 
EarI CTCTTCn'nnn_

Cuts at:      0     256     559    1136    1212 
   Size:        256     303     577      76

  Fragments arranged by size: 

                 577     303     256      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     612     724    1212 
   Size:        127     485     112     488

  Fragments arranged by size: 

                 488     485     127     112
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     545     869    1184    1212 
   Size:        545     324     315      28

  Fragments arranged by size: 

                 545     324     315      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     119     120     186     208     874     926    1122    1207 
   Size:        119       1      66      22     666      52     196      85

Cuts at:   1207    1212 
   Size:          5

  Fragments arranged by size: 

                 666     196     119      85      66      52      22       5
                   1
 
FspI TGC'GCA

Cuts at:      0     661    1212 
   Size:        661     551
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     313     332     345     364     373     405     861     893 
   Size:        313      19      13      19       9      32     456      32

Cuts at:    893    1212 
   Size:        319

  Fragments arranged by size: 

                 456     319     313      32      32      19      19      13
                   9
 
HindIII A'AGCT_T

Cuts at:      0     176    1212 
   Size:        176    1036
 
MfeI C'AATT_G

Cuts at:      0     715    1212 
   Size:        715     497
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     548    1151    1212 
   Size:        356     192     603      61

  Fragments arranged by size: 

                 603     356     192      61
 
MscI TGG'CCA

Cuts at:      0     907    1212 
   Size:        907     305
 
MslI CAynn'nnrTG

Cuts at:      0     476    1212 
   Size:        476     736
 
MspA1I CmG'CkG

Cuts at:      0     141     899    1212 
   Size:        141     758     313

  Fragments arranged by size: 

                 758     313     141
 
NspI r_CATG'y

Cuts at:      0     431    1212 
   Size:        431     781
 
PasI CC'CwG_GG

Cuts at:      0     198     212    1032    1212 
   Size:        198      14     820     180

  Fragments arranged by size: 

                 820     198     180      14
 
PciI A'CATG_T

Cuts at:      0     427    1212 
   Size:        427     785
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     144     720     736    1212 
   Size:        144     576      16     476

  Fragments arranged by size: 

                 576     476     144      16
 
PfoI T'CCnGG_A

Cuts at:      0     272    1212 
   Size:        272     940
 
PpuMI rG'GwC_Cy

Cuts at:      0     186    1212 
   Size:        186    1026
 
PspOMI G'GGCC_C

Cuts at:      0     119     925    1212 
   Size:        119     806     287

  Fragments arranged by size: 

                 806     287     119
 
PstI C_TGCA'G

Cuts at:      0     456     566     990    1212 
   Size:        456     110     424     222

  Fragments arranged by size: 

                 456     424     222     110
 
PvuII CAG'CTG

Cuts at:      0     899    1212 
   Size:        899     313
 
SapI GCTCTTCn'nnn_

Cuts at:      0     559    1212 
   Size:        559     653
 
SbfI CC_TGCA'GG

Cuts at:      0     456    1212 
   Size:        456     756
 
ScaI AGT'ACT

Cuts at:      0    1200    1212 
   Size:       1200      12
 
SfcI C'TryA_G

Cuts at:      0     452     562     621     894     986    1212 
   Size:        452     110      59     273      92     226

  Fragments arranged by size: 

                 452     273     226     110      92      59
 
SmaI CCC'GGG

Cuts at:      0     592    1212 
   Size:        592     620
 
StuI AGG'CCT

Cuts at:      0     638    1049    1212 
   Size:        638     411     163

  Fragments arranged by size: 

                 638     411     163
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      18     925    1212 
   Size:         18     907     287

  Fragments arranged by size: 

                 907     287      18
 
TatI w'GTAC_w

Cuts at:      0    1198    1212 
   Size:       1198      14
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      14      46     500     532    1212 
   Size:         14      32     454      32     680

  Fragments arranged by size: 

                 680     454      32      32      14
 
Tth111I GACn'n_nGTC

Cuts at:      0     327    1212 
   Size:        327     885
 
XmaI C'CCGG_G

Cuts at:      0     590    1212 
   Size:        590     622
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI     AvaI    BamHI     BanI
    BanII Bme1580I     BpmI     BsaI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BsrDI    BssSI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI     DrdI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I     FspI    Hin4I  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NspI     PasI     PciI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SapI     SbfI     ScaI     SfcI     SmaI     StuI
    TaqII     TatI     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI     XbaI     XcmI     XhoI     XmnI     ZraI
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