Restriction map for pF1KB6643
 (Linear) (Six-base) MAPSORT of: KIBB6643.seq  Check: 1236  from: 1  to: 1161



KIBB6643.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AccI GT'mk_AC

Cuts at:      0     176    1161 
   Size:        176     985
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299    1161 
   Size:        299     862
 
AhdI GACnn_n'nnGTC

Cuts at:      0     542    1161 
   Size:        542     619
 
ApaI G_GGCC'C

Cuts at:      0     778    1161 
   Size:        778     383
 
ApoI r'AATT_y

Cuts at:      0     882    1161 
   Size:        882     279
 
AvaI C'yCGr_G

Cuts at:      0      21    1161 
   Size:         21    1140
 
BamHI G'GATC_C

Cuts at:      0     472    1161 
   Size:        472     689
 
BanII G_rGCy'C

Cuts at:      0     778    1161 
   Size:        778     383
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     809    1161 
   Size:        809     352
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1043    1161 
   Size:       1043     118
 
BfrBI ATG'CAT

Cuts at:      0     618     910    1161 
   Size:        618     292     251

  Fragments arranged by size: 

                 618     292     251
 
Bme1580I G_kGCm'C

Cuts at:      0     778    1161 
   Size:        778     383
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     299    1161 
   Size:        299     862
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     830    1161 
   Size:        281     549     331

  Fragments arranged by size: 

                 549     331     281
 
BsaBI GATnn'nnATC

Cuts at:      0     200    1161 
   Size:        200     961
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     755    1161 
   Size:        755     406
 
BseYI C'CCAG_C

Cuts at:      0     947    1109    1161 
   Size:        947     162      52

  Fragments arranged by size: 

                 947     162      52
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329    1161 
   Size:        329     832
 
Bsp1286I G_dGCh'C

Cuts at:      0     778    1161 
   Size:        778     383
 
BsrBI CCG'CTC

Cuts at:      0     278    1161 
   Size:        278     883
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     159    1161 
   Size:        159    1002
 
BstEII G'GTnAC_C

Cuts at:      0     976    1161 
   Size:        976     185
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     382    1161 
   Size:        382     779
 
BstYI r'GATC_y

Cuts at:      0     472    1161 
   Size:        472     689
 
BstZ17I GTA'TAC

Cuts at:      0     177    1161 
   Size:        177     984
 
BtgI C'CryG_G

Cuts at:      0     468     476    1161 
   Size:        468       8     685

  Fragments arranged by size: 

                 685     468       8
 
DraIII CAC_nnn'GTG

Cuts at:      0    1015    1161 
   Size:       1015     146
 
EaeI y'GGCC_r

Cuts at:      0     372     894    1161 
   Size:        372     522     267

  Fragments arranged by size: 

                 522     372     267
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     677    1161 
   Size:        677     484
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     299     830    1161 
   Size:        281      18     531     331

  Fragments arranged by size: 

                 531     331     281      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     848    1161 
   Size:        848     313
 
EcoO109I rG'GnC_Cy

Cuts at:      0     256     337     409     774     841    1024    1161 
   Size:        256      81      72     365      67     183     137

  Fragments arranged by size: 

                 365     256     183     137      81      72      67
 
EcoRV GAT'ATC

Cuts at:      0     940    1161 
   Size:        940     221
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     528     560    1161 
   Size:        528      32     601

  Fragments arranged by size: 

                 601     528      32
 
HincII GTy'rAC

Cuts at:      0     714    1161 
   Size:        714     447
 
HpaI GTT'AAC

Cuts at:      0     714    1161 
   Size:        714     447
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      53    1161 
   Size:         53    1108
 
MscI TGG'CCA

Cuts at:      0     896    1161 
   Size:        896     265
 
MslI CAynn'nnrTG

Cuts at:      0     451    1161 
   Size:        451     710
 
MspA1I CmG'CkG

Cuts at:      0      55    1161 
   Size:         55    1106
 
NcoI C'CATG_G

Cuts at:      0     468     476    1161 
   Size:        468       8     685

  Fragments arranged by size: 

                 685     468       8
 
NdeI CA'TA_TG

Cuts at:      0     395     728    1161 
   Size:        395     333     433

  Fragments arranged by size: 

                 433     395     333
 
NsiI A_TGCA'T

Cuts at:      0     620     912    1161 
   Size:        620     292     249

  Fragments arranged by size: 

                 620     292     249
 
NspI r_CATG'y

Cuts at:      0     312     357     519    1161 
   Size:        312      45     162     642

  Fragments arranged by size: 

                 642     312     162      45
 
PasI CC'CwG_GG

Cuts at:      0     964    1161 
   Size:        964     197
 
PfoI T'CCnGG_A

Cuts at:      0     258    1161 
   Size:        258     903
 
PpuMI rG'GwC_Cy

Cuts at:      0     256     337     409     841    1024    1161 
   Size:        256      81      72     432     183     137

  Fragments arranged by size: 

                 432     256     183     137      81      72
 
PspOMI G'GGCC_C

Cuts at:      0     774    1161 
   Size:        774     387
 
ScaI AGT'ACT

Cuts at:      0     790    1161 
   Size:        790     371
 
SfcI C'TryA_G

Cuts at:      0     756    1161 
   Size:        756     405
 
SphI G_CATG'C

Cuts at:      0     519    1161 
   Size:        519     642
 
SspI AAT'ATT

Cuts at:      0     868    1161 
   Size:        868     293
 
StuI AGG'CCT

Cuts at:      0    1119    1161 
   Size:       1119      42
 
StyI C'CwwG_G

Cuts at:      0     468     476     603     675     717    1161 
   Size:        468       8     127      72      42     444

  Fragments arranged by size: 

                 468     444     127      72      42       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     249    1161 
   Size:        249     912
 
TatI w'GTAC_w

Cuts at:      0     522     788    1161 
   Size:        522     266     373

  Fragments arranged by size: 

                 522     373     266
 
Tth111I GACn'n_nGTC

Cuts at:      0     839    1055    1161 
   Size:        839     216     106

  Fragments arranged by size: 

                 839     216     106
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     826    1141    1161 
   Size:        826     315      20

  Fragments arranged by size: 

                 826     315      20
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     ApaI     ApoI     AvaI    BamHI    BanII
     BbsI    BciVI    BfrBI Bme1580I     BplI     BpmI    BsaBI    BseRI
    BseYI     BsgI Bsp1286I    BsrBI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I     BtgI   DraIII     EaeI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRV    Hin4I   HincII     HpaI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NsiI     NspI     PasI     PfoI    PpuMI   PspOMI
     ScaI     SfcI     SphI     SspI     StuI     StyI    TaqII     TatI
  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI     BclI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EagI     EarI  EcoICRI    EcoRI     FalI     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SwaI     TstI     XbaI     XhoI     XmaI
     XmnI     ZraI
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