Restriction map for pF1KB6618
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AviII       AvrII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FseI        FspI        HaeII       Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         677  CGCG                 CG^CG
AlwNI        1169  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          175  ATTAAT               AT^TAAT
AsnI          175  ATTAAT               AT^TAAT
Asp700I      1218  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1124  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          932  CTCGAG               C^[CT]CG[GA]G
BamHI         981  GGATCC               G^GATCC
BbsI           30  GAAGAC               GAAGAC(2/6)
BcnI          652  CCCGG                CC^[GC]GG
BpuAI          30  GAAGAC               GAAGAC(2/6)
BseRI        1277  CTCCTC               GAGGAG(10/8)
BsoBI         932  CTCGAG               C^[CT]CG[GA]G
BspMI         492  GCAGGT               ACCTGC(4/8)
BsrDI         455  CATTGC               GCAATG(2/0)
BstUI         677  CGCG                 CG^CG
CfoI          676  GCGC                 GCG^C
DraII         320  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        668  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          499  GAAGAG               CTCTTC(1/4)
EcoO109I      320  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV        1099  GATATC               GAT^ATC
FauI          678  GCGGG                CCCGC(4/6)
HgaI         1160  GCGTC                GACGC(5/10)
HhaI          676  GCGC                 GCG^C
HinP1I        676  GCGC                 G^CGC
Ksp632I       499  GAAGAG               CTCTTC(1/4)
MslI         1121  CACGACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         25  CAGCGG               C[AC]G^C[GT]G
MvnI          677  CGCG                 CG^CG
NciI          652  CCCGG                CC^[GC]GG
PaeR7I        932  CTCGAG               C^TCGAG
PflFI        1124  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         320  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI         175  ATTAAT               AT^TAAT
PstI          242  CTGCAG               CTGCA^G
SfcI          242  CTGCAG               C^T[GA][CT]AG
SspI          940  AATATT               AAT^ATT
Tth111I      1124  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1041  TCTAGA               T^CTAGA
XhoI          932  CTCGAG               C^TCGAG
XmnI         1218  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AciI           27  GCGG                 CCGC(-3/-1)
              678  GCGG                
BalI          788  TGGCCA               TGG^CCA
              956  TGGCCA              
BanI           16  GGTGCC               G^G[CT][GA]CC
              387  GGCACC              
BbvI          241  GCTGC                GCAGC(8/12)
              636  GCTGC               
BclI          378  TGATCA               T^GATCA
              905  TGATCA              
BfaI          125  CTAG                 C^TAG
             1042  CTAG                
BpmI          259  CTCCAG               CTGGAG(16/14)
              866  CTGGAG              
BsgI         1164  CTGCAC               GTGCAG(16/14)
              883  GTGCAG              
Bsp1407I      292  TGTACA               T^GTACA
              735  TGTACA              
BsrGI         292  TGTACA               T^GTACA
              735  TGTACA              
BstXI         223  CCAGCACTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              356  CCAAGTTTGTGG        
BstYI         724  GGATCT               [GA]^GATC[CT]
              981  GGATCC              
EaeI          788  TGGCCA               [CT]^GGCC[GA]
              956  TGGCCA              
EcoT14I       609  CCTTGG               C^C[AT][AT]GG
              626  CCAAGG              
FbaI          378  TGATCA               T^GATCA
              905  TGATCA              
Fnu4HI        241  GCTGC                GC^[ACGT]GC
              636  GCTGC               
HapII           4  CCGG                 C^CGG
              653  CCGG                
HpaII           4  CCGG                 C^CGG
              653  CCGG                
HphI          751  GGTGA                GGTGA(8/7)
              871  GGTGA               
ItaI          241  GCTGC                GC^[ACGT]GC
              636  GCTGC               
MaeI          125  CTAG                 C^TAG
             1042  CTAG                
MaeII         341  ACGT                 A^CGT
              746  ACGT                
MflI          724  GGATCT               [GA]^GATC[CT]
              981  GGATCC              
MluNI         788  TGGCCA               TGG^CCA
              956  TGGCCA              
MscI          788  TGGCCA               TGG^CCA
              956  TGGCCA              
MspI            4  CCGG                 C^CGG
              653  CCGG                
NlaIII        486  CATG                 CATG^
             1287  CATG                
SacI           68  GAGCTC               GAGCT^C
              181  GAGCTC              
SmlI          166  CTCAAG               C^T[CT][GA]AG
              932  CTCGAG              
SspBI         292  TGTACA               T^GTACA
              735  TGTACA              
StyI          609  CCTTGG               C^C[AT][AT]GG
              626  CCAAGG              
TseI          241  GCTGC                G^C[AT]GC
              636  GCTGC               
Tsp45I        514  GTCAC                ^GT[GC]AC
              872  GTGAC               
XhoII         724  GGATCT               [GA]^GATC[CT]
              981  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AspHI       AvaII       BanII       BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      FokI        HaeIII      
HinfI       MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TfiI        Tru9I       Tsp509I     TspRI       
TthHB8I     XcmI        
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