Restriction map for pF1KB6618
 (Linear) (Six-base) MAPSORT of: KIBB6618.seq  Check: 4568  from: 1  to: 1299



KIBB6618.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     483    1299 
   Size:        483     816
 
AleI CACnn'nnGTG

Cuts at:      0    1125    1299 
   Size:       1125     174
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1174    1299 
   Size:       1174     125
 
ApoI r'AATT_y

Cuts at:      0     443    1024    1264    1299 
   Size:        443     581     240      35

  Fragments arranged by size: 

                 581     443     240      35
 
AseI AT'TA_AT

Cuts at:      0     176    1299 
   Size:        176    1123
 
AvaI C'yCGr_G

Cuts at:      0     932    1299 
   Size:        932     367
 
BamHI G'GATC_C

Cuts at:      0     981    1299 
   Size:        981     318
 
BanI G'GyrC_C

Cuts at:      0      16     387    1299 
   Size:         16     371     912

  Fragments arranged by size: 

                 912     371      16
 
BanII G_rGCy'C

Cuts at:      0      72     185     762     849    1299 
   Size:         72     113     577      87     450

  Fragments arranged by size: 

                 577     450     113      87      72
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      37    1299 
   Size:         37    1262
 
BclI T'GATC_A

Cuts at:      0     378     905    1299 
   Size:        378     527     394

  Fragments arranged by size: 

                 527     394     378
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     887    1299 
   Size:        244     643     412

  Fragments arranged by size: 

                 643     412     244
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151    1299 
   Size:        151    1148
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1268    1299 
   Size:       1268      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     904    1149    1299 
   Size:        904     245     150

  Fragments arranged by size: 

                 904     245     150
 
BsiHKAI G_wGCw'C

Cuts at:      0      72     118     185    1197    1299 
   Size:         72      46      67    1012     102

  Fragments arranged by size: 

                1012     102      72      67      46
 
Bsp1286I G_dGCh'C

Cuts at:      0      72     118     185     762     849    1197    1299 
   Size:         72      46      67     577      87     348     102

  Fragments arranged by size: 

                 577     348     102      87      72      67      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     483    1299 
   Size:        483     816
 
BsrDI GCAATG_nn'

Cuts at:      0     454    1299 
   Size:        454     845
 
BsrGI T'GTAC_A

Cuts at:      0     292     735    1299 
   Size:        292     443     564

  Fragments arranged by size: 

                 564     443     292
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     230     363    1299 
   Size:        230     133     936

  Fragments arranged by size: 

                 936     230     133
 
BstYI r'GATC_y

Cuts at:      0     724     981    1299 
   Size:        724     257     318

  Fragments arranged by size: 

                 724     318     257
 
DraIII CAC_nnn'GTG

Cuts at:      0     673    1299 
   Size:        673     626
 
EaeI y'GGCC_r

Cuts at:      0     788     956    1299 
   Size:        788     168     343

  Fragments arranged by size: 

                 788     343     168
 
EarI CTCTTCn'nnn_

Cuts at:      0     494    1299 
   Size:        494     805
 
EcoICRI GAG'CTC

Cuts at:      0      70     183    1299 
   Size:         70     113    1116

  Fragments arranged by size: 

                1116     113      70
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     887    1299 
   Size:        244     643     412

  Fragments arranged by size: 

                 643     412     244
 
EcoO109I rG'GnC_Cy

Cuts at:      0     321    1299 
   Size:        321     978
 
EcoRV GAT'ATC

Cuts at:      0    1101    1299 
   Size:       1101     198
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     826    1242    1299 
   Size:        826     416      57

  Fragments arranged by size: 

                 826     416      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1059    1091    1299 
   Size:       1059      32     208

  Fragments arranged by size: 

                1059     208      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     878    1030    1299 
   Size:        878     152     269

  Fragments arranged by size: 

                 878     269     152
 
MscI TGG'CCA

Cuts at:      0     790     958    1299 
   Size:        790     168     341

  Fragments arranged by size: 

                 790     341     168
 
MslI CAynn'nnrTG

Cuts at:      0    1125    1299 
   Size:       1125     174
 
MspA1I CmG'CkG

Cuts at:      0      27    1299 
   Size:         27    1272
 
PpuMI rG'GwC_Cy

Cuts at:      0     321    1299 
   Size:        321     978
 
PspXI vC'TCGA_Gb

Cuts at:      0     932    1299 
   Size:        932     367
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     260     292    1299 
   Size:        260      32    1007

  Fragments arranged by size: 

                1007     260      32
 
PstI C_TGCA'G

Cuts at:      0     246    1299 
   Size:        246    1053
 
SacI G_AGCT'C

Cuts at:      0      72     185    1299 
   Size:         72     113    1114

  Fragments arranged by size: 

                1114     113      72
 
SfcI C'TryA_G

Cuts at:      0     242    1299 
   Size:        242    1057
 
SmlI C'TyrA_G

Cuts at:      0     166     932    1299 
   Size:        166     766     367

  Fragments arranged by size: 

                 766     367     166
 
SspI AAT'ATT

Cuts at:      0     942    1299 
   Size:        942     357
 
StyI C'CwwG_G

Cuts at:      0     609     626    1299 
   Size:        609      17     673

  Fragments arranged by size: 

                 673     609      17
 
TatI w'GTAC_w

Cuts at:      0     292     735    1299 
   Size:        292     443     564

  Fragments arranged by size: 

                 564     443     292
 
Tth111I GACn'n_nGTC

Cuts at:      0    1127    1299 
   Size:       1127     172
 
XbaI T'CTAG_A

Cuts at:      0    1041    1299 
   Size:       1041     258
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      69     227     363     492    1299 
   Size:         69     158     136     129     807

  Fragments arranged by size: 

                 807     158     136     129      69
 
XhoI C'TCGA_G

Cuts at:      0     932    1299 
   Size:        932     367
 
XmnI GAAnn'nnTTC

Cuts at:      0    1222    1299 
   Size:       1222      77
 

 Enzymes that do cut:

     AarI     AleI    AlwNI     ApoI     AseI     AvaI    BamHI     BanI
    BanII     BbsI     BclI     BpmI    BpuEI    BseRI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrDI    BsrGI    BstXI    BstYI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I     MmeI
     MscI     MslI   MspA1I    PpuMI    PspXI     PsrI     PstI     SacI
     SfcI     SmlI     SspI     StyI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EciI    EcoNI
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI
     XmaI     ZraI
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