Restriction map for
pF1KB6593
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvrII BbeI BbrPI BcgI BclI
BfrI BglII BlnI BpmI BsaAI
BsaHI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI ScaI SexAI SfoI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 271 CGCG CG^CG
AcsI 613 GAATTT [GA]^AATT[CT]
AflIII 473 ACACGT A^C[GA][CT]GT
AgeI 496 ACCGGT A^CCGGT
ApaI 526 GGGCCC GGGCC^C
ApoI 613 GAATTT [GA]^AATT[CT]
AvaI 994 CCCGAG C^[CT]CG[GA]G
AvaII 990 GGACC G^G[AT]CC
AviII 320 TGCGCA TGC^GCA
BalI 900 TGGCCA TGG^CCA
BamHI 1035 GGATCC G^GATCC
BbvCI 730 GCTGAGG CCTCAGC(-5/-2)
BciVI 209 GTATCC GTATCC(6/5)
BfaI 1015 CTAG C^TAG
BlpI 932 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 932 GCTCAGC GC^T[ACGT]AGC
BsaWI 496 ACCGGT [AT]^CCGG[AT]
BsgI 258 CTGCAC GTGCAG(16/14)
BsmAI 56 GAGAC GTCTC(1/5)
BsoBI 994 CCCGAG C^[CT]CG[GA]G
BsrDI 327 CATTGC GCAATG(2/0)
BsrFI 496 ACCGGT [GA]^CCGG[CT]
BstAPI 718 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1055 TTCGAA TT^CGAA
BstUI 271 CGCG CG^CG
CelII 932 GCTCAGC GC^T[ACGT]AGC
CfoI 321 GCGC GCG^C
Cfr10I 496 ACCGGT [GA]^CCGG[CT]
DraII 989 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 390 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 989 AGGACCC [GA]G^G[ACGT]CC[CT]
FspI 320 TGCGCA TGC^GCA
HhaI 321 GCGC GCG^C
HinP1I 321 GCGC G^CGC
MaeI 1015 CTAG C^TAG
MluNI 900 TGGCCA TGG^CCA
MscI 900 TGGCCA TGG^CCA
MvnI 271 CGCG CG^CG
NspV 1055 TTCGAA TT^CGAA
PflMI 196 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 496 ACCGGT A^CCGGT
PleI 763 GACTC GAGTC(4/5)
PpuMI 989 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 713 CAGCTG CAG^CTG
SbfI 882 CCTGCAGG CCTGCA^GG
SfiI 1077 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1055 TTCGAA TT^CGAA
SmlI 391 CTCAAG C^T[CT][GA]AG
Sse8387I 882 CCTGCAGG CCTGCA^GG
TaqI 1056 TCGA T^CGA
TthHB8I 1056 TCGA T^CGA
Van91I 196 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 197 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
719 CAGCTTCTG
Asp700I 494 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1048 GAAGCCATTC
BanI 580 GGTGCC G^G[CT][GA]CC
598 GGTGCC
BanII 526 GGGCCC G[GA]GC[CT]^C
1068 GGGCTC
BbsI 760 GAAGAC GAAGAC(2/6)
1120 GAAGAC
BcnI 704 CCCGG CC^[GC]GG
737 CCGGG
BmyI 526 GGGCCC G[AGT]GC[ACT]^C
1068 GGGCTC
BpuAI 760 GAAGAC GAAGAC(2/6)
1120 GAAGAC
BsaBI 318 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
786 GATGACCATC
BsmFI 754 GGGAC GGGAC(10/14)
879 GTCCC
Bsp1286I 526 GGGCCC G[AGT]GC[ACT]^C
1068 GGGCTC
FauI 122 CCCGC CCCGC(4/6)
708 GCGGG
HincII 97 GTCAAC GT[CT]^[GA]AC
412 GTTGAC
HindII 97 GTCAAC GT[CT]^[GA]AC
412 GTTGAC
MaeIII 511 GTGAC ^GT[ACGT]AC
605 GTGAC
MamI 318 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
786 GATGACCATC
NciI 704 CCCGG CC^[GC]GG
737 CCGGG
NlaIII 585 CATG CATG^
870 CATG
SapI 673 GAAGAGC GCTCTTC(1/4)
850 GAAGAGC
TfiI 309 GAATC G^A[AT]TC
910 GAATC
Tsp45I 511 GTGAC ^GT[GC]AC
605 GTGAC
Tsp509I 424 AATT ^AATT
614 AATT
XcmI 798 CCAGCAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
929 CCAGCTCAGCTATGG
XmnI 494 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1048 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI BbvI BglI
BsaJI BseRI BsiYI BslI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NdeII NlaIV PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI TspRI XhoII