Restriction map for pF1KB6593
 (Circular) (Six-base) MAPSORT of: pF1KB6593.seq  Check: 4594  from: 1  to: 4244



pF1KB6593  4244 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AccI GT'mk_AC

Cuts at:   1282    1282 
   Size:       4244
 
Acc65I G'GTAC_C

Cuts at:   1260    1260 
   Size:       4244
 
AclI AA'CG_TT

Cuts at:   4084    4084 
   Size:       4244
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    418     887     910    1096    2069    2501    3248    3516     418 
   Size:        469      23     186     973     432     747     268    1146

  Fragments arranged by size: 

                1146     973     747     469     432     268     186      23
            
 
AflIII A'CryG_T

Cuts at:    581    1596    2700    3677     581 
   Size:       1015    1104     977    1148

  Fragments arranged by size: 

                1148    1104    1015     977
 
AgeI A'CCGG_T

Cuts at:    604    2632     604 
   Size:       2028    2216
 
AleI CACnn'nnGTG

Cuts at:    127     127 
   Size:       4244
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1090    1122    1090 
   Size:         32    4212
 
AlwNI CAG_nnn'CTG

Cuts at:    310     832    1549    3116     310 
   Size:        522     717    1567    1438

  Fragments arranged by size: 

                1567    1438     717     522
 
ApaI G_GGCC'C

Cuts at:    638     638 
   Size:       4244
 
ApaLI G'TGCA_C

Cuts at:   3014    3014 
   Size:       4244
 
ApoI r'AATT_y

Cuts at:    721    1248    3597    3853     721 
   Size:        527    2349     256    1112

  Fragments arranged by size: 

                2349    1112     527     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4244
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4244
 
AvaI C'yCGr_G

Cuts at:   1102    1264    1102 
   Size:        162    4082
 
BamHI G'GATC_C

Cuts at:   1143    1269    1143 
   Size:        126    4118
 
BanI G'GyrC_C

Cuts at:    688     706    1260    1674    1922    1957     688 
   Size:         18     554     414     248      35    2975

  Fragments arranged by size: 

                2975     554     414     248      35      18
 
BanII G_rGCy'C

Cuts at:    638    1180    1258    2288     638 
   Size:        542      78    1030    2594

  Fragments arranged by size: 

                2594    1030     542      78
 
BbeI G_GCGC'C

Cuts at:   1678    1926    1678 
   Size:        248    3996
 
BbsI GAAGACnn'nnnn_

Cuts at:    875    1235     875 
   Size:        360    3884
 
BbvCI CC'TCA_GC

Cuts at:    839     839 
   Size:       4244
 
BciVI GTATCCnnnnn_n'

Cuts at:    328    2142    2903    4244     328 
   Size:       1814     761    1341     328

  Fragments arranged by size: 

                1814    1341     761     328
 
BclI T'GATC_A

Cuts at:   1764    1764 
   Size:       4244
 
BglI GCCn_nnn'nGGC

Cuts at:    391     633     817    1192     391 
   Size:        242     184     375    3443

  Fragments arranged by size: 

                3443     375     242     184
 
BglII A'GATC_T

Cuts at:   1759    3796    1759 
   Size:       2037    2207
 
BlpI GC'TnA_GC

Cuts at:   1041    1356    1041 
   Size:        315    3929
 
Bme1580I G_kGCm'C

Cuts at:    638    1869    1962    3018     638 
   Size:       1231      93    1056    1864

  Fragments arranged by size: 

                1864    1231    1056      93
 
BmgBI CAC'GTC

Cuts at:   1089    1089 
   Size:       4244
 
BmrI ACTGGGnnnn_n'

Cuts at:    448     632     731     869     935    1456    1537    1872    4016 
   Size:        184      99     138      66     521      81     335    2144

Cuts at:   4016     448 
   Size:        676

  Fragments arranged by size: 

                2144     676     521     335     184     138      99      81
                  66
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2611    2611 
   Size:       4244
 
Bpu10I CC'TnA_GC

Cuts at:    749     839     940     749 
   Size:         90     101    4053

  Fragments arranged by size: 

                4053     101      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    484    1420    2299    2791    3089     484 
   Size:        936     879     492     298    1639

  Fragments arranged by size: 

                1639     936     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3948    3948 
   Size:       4244
 
BsaAI yAC'GTr

Cuts at:   1597    1597 
   Size:       4244
 
BsaBI GATnn'nnATC

Cuts at:      6     430     898    1758    3795       6 
   Size:        424     468     860    2037     455

  Fragments arranged by size: 

                2037     860     468     455     424
 
BsaHI Gr'CG_yC

Cuts at:   1675    1923    2625    4121    1675 
   Size:        248     702    1496    1798

  Fragments arranged by size: 

                1798    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    604    1433    1954    2632    2906    3053    3464     604 
   Size:        829     521     678     274     147     411    1384

  Fragments arranged by size: 

                1384     829     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    612     642     612 
   Size:         30    4214
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    559     817     820    1087     559 
   Size:        258       3     267    3716

  Fragments arranged by size: 

                3716     267     258       3
 
BseYI C'CCAG_C

Cuts at:    191     696    1669    2427    3004    3500     191 
   Size:        505     973     758     577     496     935

  Fragments arranged by size: 

                 973     935     758     577     505     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    351     351 
   Size:       4244
 
BsiEI CG_ry'CG

Cuts at:    104    1786    1832    3040     104 
   Size:       1682      46    1208    1308

  Fragments arranged by size: 

                1682    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1258    2036    2226    3018    1258 
   Size:        778     190     792    2484

  Fragments arranged by size: 

                2484     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    638    1180    1258    1869    1962    2036    2226    2288    3018 
   Size:        542      78     611      93      74     190      62     730

Cuts at:   3018     638 
   Size:       1864

  Fragments arranged by size: 

                1864     730     611     542     190      93      78      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3464    3464 
   Size:       4244
 
BspHI T'CATG_A

Cuts at:   3420    3420 
   Size:       4244
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1294    1508    1810    2191    1294 
   Size:        214     302     381    3347

  Fragments arranged by size: 

                3347     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2536    2590    4074    4239      50 
   Size:       2486      54    1484     165      55

  Fragments arranged by size: 

                2486    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    434    2156     434 
   Size:       1722    2522
 
BsrFI r'CCGG_y

Cuts at:    604    2242    2423    2632     604 
   Size:       1638     181     209    2216

  Fragments arranged by size: 

                2216    1638     209     181
 
BssSI C'ACGA_G

Cuts at:   2515    2873    2515 
   Size:        358    3886
 
BstAPI GCAn_nnn'nTGC

Cuts at:    832    1621     832 
   Size:        789    3455
 
BstBI TT'CG_AA

Cuts at:   1164    2605    1164 
   Size:       1441    2803
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     114 
   Size:       4244
 
BstYI r'GATC_y

Cuts at:      1     474     732    1143    1269    1736    1759    2094    2340 
   Size:        473     258     411     126     467      23     335     246

Cuts at:   2340    3352    3438    3450    3796       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     473     467     449     411     346     335     258
                 246     126      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4244
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2194    2375    2538    2194 
   Size:        181     163    3900

  Fragments arranged by size: 

                3900     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1487    1487 
   Size:       4244
 
DraI TTT'AAA

Cuts at:   1243    1243 
   Size:       4244
 
DrdI GACnn_nn'nnGTC

Cuts at:   1950    2808    1950 
   Size:        858    3386
 
EaeI y'GGCC_r

Cuts at:    312     918    1008    1829    2003    2394    2421    3766     312 
   Size:        606      90     821     174     391      27    1345     790

  Fragments arranged by size: 

                1345     821     790     606     391     174      90      27
            
 
EagI C'GGCC_G

Cuts at:   1829    1829 
   Size:       4244
 
EarI CTCTTCn'nnn_

Cuts at:    536     776     953    2267    2477     536 
   Size:        240     177    1314     210    2303

  Fragments arranged by size: 

                2303    1314     240     210     177
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1030    2452    2762    2908    4054    1030 
   Size:       1422     310     146    1146    1220

  Fragments arranged by size: 

                1422    1220    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1256    1256 
   Size:       4244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    418     887     910    1096    2069    2501    3248    3516     418 
   Size:        469      23     186     973     432     747     268    1146

  Fragments arranged by size: 

                1146     973     747     469     432     268     186      23
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    502     502 
   Size:       4244
 
EcoO109I rG'GnC_Cy

Cuts at:   1098    1383    1098 
   Size:        285    3959
 
EcoRI G'AATT_C

Cuts at:   1248    1248 
   Size:       4244
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    378    2263     378 
   Size:       1885    2359
 
FspI TGC'GCA

Cuts at:    430    2025     430 
   Size:       1595    2649
 
FspAI rTGC'GCAy

Cuts at:    430     430 
   Size:       4244
 
HaeII r_GCGC'y

Cuts at:   1678    1926    2948    3927    1678 
   Size:        248    1022     979    1995

  Fragments arranged by size: 

                1995    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    612     644    1024    1056    2086    2116    2118    2148     612 
   Size:         32     380      32    1030      30       2      30    2708

  Fragments arranged by size: 

                2708    1030     380      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    207     522    1283     207 
   Size:        315     761    3168

  Fragments arranged by size: 

                3168     761     315
 
KasI G'GCGC_C

Cuts at:   1674    1922    1674 
   Size:        248    3996
 
KpnI G_GTAC'C

Cuts at:   1264    1264 
   Size:       4244
 
MluI A'CGCG_T

Cuts at:   3677    3677 
   Size:       4244
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:     95     366    2915    3099      95 
   Size:        271    2549     184    1240

  Fragments arranged by size: 

                2549    1240     271     184
 
MscI TGG'CCA

Cuts at:   1010    2005    1010 
   Size:        995    3249
 
MslI CAynn'nnrTG

Cuts at:    127     379     982    2233    2360     127 
   Size:        252     603    1251     127    2011

  Fragments arranged by size: 

                2011    1251     603     252     127
 
MspA1I CmG'CkG

Cuts at:    789     795     823    1355    1669    2029    3042    3287     789 
   Size:          6      28     532     314     360    1013     245    1746

  Fragments arranged by size: 

                1746    1013     532     360     314     245      28       6
            
 
NaeI GCC'GGC

Cuts at:   2425    2425 
   Size:       4244
 
NarI GG'CG_CC

Cuts at:   1675    1923    1675 
   Size:        248    3996
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4244
 
NgoMIV G'CCGG_C

Cuts at:   2423    2423 
   Size:       4244
 
NspI r_CATG'y

Cuts at:   1297    1797    2704    1297 
   Size:        500     907    2837

  Fragments arranged by size: 

                2837     907     500
 
PciI A'CATG_T

Cuts at:   2700    2700 
   Size:       4244
 
PflMI CCAn_nnn'nTGG

Cuts at:    310     310 
   Size:       4244
 
PmeI GTTT'AAAC

Cuts at:   1243    1243 
   Size:       4244
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    594     626    3383    3415     594 
   Size:         32    2757      32    1423

  Fragments arranged by size: 

                2757    1423      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1098    1098 
   Size:       4244
 
PspOMI G'GGCC_C

Cuts at:    634     634 
   Size:       4244
 
PstI C_TGCA'G

Cuts at:    188     828     995    1291    1976     188 
   Size:        640     167     296     685    2456

  Fragments arranged by size: 

                2456     685     640     296     167
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4244
 
PvuII CAG'CTG

Cuts at:    823    1669    2029     823 
   Size:        846     360    3038

  Fragments arranged by size: 

                3038     846     360
 
RsrII CG'GwC_CG

Cuts at:   2439    2439 
   Size:       4244
 
SacI G_AGCT'C

Cuts at:   1258    1258 
   Size:       4244
 
SalI G'TCGA_C

Cuts at:   1281    1281 
   Size:       4244
 
SapI GCTCTTCn'nnn_

Cuts at:    776     953    2267    2477     776 
   Size:        177    1314     210    2543

  Fragments arranged by size: 

                2543    1314     210     177
 
SbfI CC_TGCA'GG

Cuts at:    995    1291     995 
   Size:        296    3948
 
ScaI AGT'ACT

Cuts at:   3761    3761 
   Size:       4244
 
SfcI C'TryA_G

Cuts at:     33     184     824     991    1287    1972    2965    3156      33 
   Size:        151     640     167     296     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     640     296     191     167     151
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1192    1192 
   Size:       4244
 
SfoI GGC'GCC

Cuts at:   1676    1924    1676 
   Size:        248    3996
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4244
 
SmaI CCC'GGG

Cuts at:   1266    1266 
   Size:       4244
 
SmlI C'TyrA_G

Cuts at:    499    1399    2314    2806    3068     499 
   Size:        900     915     492     262    1675

  Fragments arranged by size: 

                1675     915     900     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4244
 
SphI G_CATG'C

Cuts at:   1297    1797    1297 
   Size:        500    3744
 
StyI C'CwwG_G

Cuts at:    107     347     758    1067    1199    1378    1732     107 
   Size:        240     411     309     132     179     354    2619

  Fragments arranged by size: 

                2619     411     354     309     240     179     132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1827    1960    2419    2628    1827 
   Size:        133     459     209    3443

  Fragments arranged by size: 

                3443     459     209     133
 
TatI w'GTAC_w

Cuts at:   3759    3759 
   Size:       4244
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    440     472     915     947    3761    3793     440 
   Size:         32     443      32    2814      32     891

  Fragments arranged by size: 

                2814     891     443      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2041    2041 
   Size:       4244
 
XbaI T'CTAG_A

Cuts at:   1275    1275 
   Size:       4244
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    913    1044    1739     913 
   Size:        131     695    3418

  Fragments arranged by size: 

                3418     695     131
 
XmaI C'CCGG_G

Cuts at:   1264    1264 
   Size:       4244
 
XmnI GAAnn'nnTTC

Cuts at:    606    1160     606 
   Size:        554    3690
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    FspAI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AscI    AvrII
     BaeI     BcgI    BfrBI     BmtI     BpmI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoRV     FseI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PshAI     PsiI
    PspXI     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     ZraI
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