Restriction map for
pF1KB6117
##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
FauI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI Sau96I SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AspHI 959 GAGCAC G[AT]GC[AT]^C
BanI 1539 GGTGCC G^G[CT][GA]CC
BbsI 1444 GAAGAC GAAGAC(2/6)
BclI 948 TGATCA T^GATCA
BglI 1375 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1444 GAAGAC GAAGAC(2/6)
BsaI 1281 GAGACC GGTCTC(1/5)
BsiHKAI 959 GAGCAC G[AT]GC[AT]^C
BsrBI 138 GAGCGG CCGCTC(-3/-3)
BssSI 489 CACGAG CACGAG(-5/-1)
BstBI 10 TTCGAA TT^CGAA
BstXI 1254 CCAAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 987 AGATCC [GA]^GATC[CT]
DsaI 290 CCGTGG C^C[GA][CT]GG
EaeI 287 TGGCCG [CT]^GGCC[GA]
EcoRV 191 GATATC GAT^ATC
FbaI 948 TGATCA T^GATCA
HaeIII 288 GGCC GG^CC
HinfI 299 GAATC G^A[ACGT]TC
MaeII 1741 ACGT A^CGT
MflI 987 AGATCC [GA]^GATC[CT]
MspA1I 812 CAGCTG C[AC]G^C[GT]G
NlaIV 1539 GGTGCC GG[ACGT]^[ACGT]CC
NspI 1628 GCATGT [GA]CATG^[CT]
NspV 10 TTCGAA TT^CGAA
PflMI 833 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1809 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 812 CAGCTG CAG^CTG
SfuI 10 TTCGAA TT^CGAA
SmlI 662 CTCAAG C^T[CT][GA]AG
TaqI 11 TCGA T^CGA
TfiI 299 GAATC G^A[AT]TC
TthHB8I 11 TCGA T^CGA
Van91I 833 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 987 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 185 GACGCC G[GA]^CG[CT]C
773 GACGCC
AlwNI 986 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1364 CAGTGACTG
BciVI 1024 GTATCC GTATCC(6/5)
1306 GTATCC
BsaBI 162 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
670 GATAACTATC
BsaHI 185 GACGCC G[GA]^CG[CT]C
773 GACGCC
BsgI 436 GTGCAG GTGCAG(16/14)
1073 GTGCAG
Bsp1407I 1084 TGTACA T^GTACA
1321 TGTACA
BsrGI 1084 TGTACA T^GTACA
1321 TGTACA
CfoI 121 GCGC GCG^C
765 GCGC
EcoT22I 573 ATGCAT ATGCA^T
1467 ATGCAT
HaeII 120 GGCGCT [GA]GCGC^[CT]
764 AGCGCC
HhaI 121 GCGC GCG^C
765 GCGC
Hin1I 185 GACGCC G[GA]^CG[CT]C
773 GACGCC
HinP1I 121 GCGC G^CGC
765 GCGC
MamI 162 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
670 GATAACTATC
MslI 427 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1470 CATCTTTATG
NsiI 573 ATGCAT ATGCA^T
1467 ATGCAT
SapI 135 GAAGAGC GCTCTTC(1/4)
1427 GCTCTTC
ScaI 1685 AGTACT AGT^ACT
1774 AGTACT
SspBI 1084 TGTACA T^GTACA
1321 TGTACA
SspI 784 AATATT AAT^ATT
1191 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HgaI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII PstI
RsaI Sau3AI ScrFI SfaNI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI