Restriction map for
pF1KB6117
(Circular) (Six-base) MAPSORT of: pF1KB6117.seq Check: 7828 from: 1 to: 4976
pF1KB6117 4976 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 2014 2014
Size: 4976
Acc65I G'GTAC_C
Cuts at: 1992 1992
Size: 4976
AclI AA'CG_TT
Cuts at: 4816 4816
Size: 4976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 902 2801 3233 3980 4248 413
Size: 489 1899 432 747 268 1141
Fragments arranged by size:
1899 1141 747 489 432 268
AflIII A'CryG_T
Cuts at: 2328 3432 4409 2328
Size: 1104 977 2895
Fragments arranged by size:
2895 1104 977
AgeI A'CCGG_T
Cuts at: 3364 3364
Size: 4976
AlwNI CAG_nnn'CTG
Cuts at: 1099 1477 2281 3848 1099
Size: 378 804 1567 2227
Fragments arranged by size:
2227 1567 804 378
ApaLI G'TGCA_C
Cuts at: 3746 3746
Size: 4976
ApoI r'AATT_y
Cuts at: 935 1322 1357 1980 4329 4585 935
Size: 387 35 623 2349 256 1326
Fragments arranged by size:
2349 1326 623 387 256 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 4976
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4976
AvaI C'yCGr_G
Cuts at: 1996 1996
Size: 4976
BamHI G'GATC_C
Cuts at: 2001 2001
Size: 4976
BanI G'GyrC_C
Cuts at: 1647 1992 2406 2654 2689 1647
Size: 345 414 248 35 3934
Fragments arranged by size:
3934 414 345 248 35
BanII G_rGCy'C
Cuts at: 1990 3020 1990
Size: 1030 3946
BbeI G_GCGC'C
Cuts at: 2410 2658 2410
Size: 248 4728
BbsI GAAGACnn'nnnn_
Cuts at: 1559 1559
Size: 4976
BciVI GTATCCnnnnn_n'
Cuts at: 1143 1425 2874 3635 4976 1143
Size: 282 1449 761 1341 1143
Fragments arranged by size:
1449 1341 1143 761 282
BclI T'GATC_A
Cuts at: 1056 2496 1056
Size: 1440 3536
BfrBI ATG'CAT
Cuts at: 683 1577 683
Size: 894 4082
BglI GCCn_nnn'nGGC
Cuts at: 1489 1489
Size: 4976
BglII A'GATC_T
Cuts at: 2491 4528 2491
Size: 2037 2939
BlpI GC'TnA_GC
Cuts at: 2088 2088
Size: 4976
Bme1580I G_kGCm'C
Cuts at: 1147 1652 2601 2694 3750 1147
Size: 505 949 93 1056 2373
Fragments arranged by size:
2373 1056 949 505 93
BmrI ACTGGGnnnn_n'
Cuts at: 1646 1785 2188 2269 2604 4748 1646
Size: 139 403 81 335 2144 1874
Fragments arranged by size:
2144 1874 403 335 139 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3343 3343
Size: 4976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 2152 3031 3523 3821 755
Size: 1397 879 492 298 1910
Fragments arranged by size:
1910 1397 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1383 4680 1383
Size: 3297 1679
BsaAI yAC'GTr
Cuts at: 2329 2329
Size: 4976
BsaBI GATnn'nnATC
Cuts at: 6 274 782 2490 4527 6
Size: 268 508 1708 2037 455
Fragments arranged by size:
2037 1708 508 455 268
BsaHI Gr'CG_yC
Cuts at: 294 882 2407 2655 3357 4853 294
Size: 588 1525 248 702 1496 417
Fragments arranged by size:
1525 1496 702 588 417 248
BsaWI w'CCGG_w
Cuts at: 2165 2686 3364 3638 3785 4196 2165
Size: 521 678 274 147 411 2945
Fragments arranged by size:
2945 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2401 3159 3736 4232 2401
Size: 758 577 496 3145
Fragments arranged by size:
3145 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1202 565
Size: 637 4339
BsiEI CG_ry'CG
Cuts at: 104 2518 2564 3772 104
Size: 2414 46 1208 1308
Fragments arranged by size:
2414 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1071 1990 2768 2958 3750 1071
Size: 919 778 190 792 2297
Fragments arranged by size:
2297 919 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1071 1147 1652 1990 2601 2694 2768 2958 3020
Size: 76 505 338 611 93 74 190 62
Cuts at: 3020 3750 1071
Size: 730 2297
Fragments arranged by size:
2297 730 611 505 338 190 93 76
74 62
BspEI T'CCGG_A
Cuts at: 4196 4196
Size: 4976
BspHI T'CATG_A
Cuts at: 4152 4152
Size: 4976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2026 2240 2542 2923 2026
Size: 214 302 381 4079
Fragments arranged by size:
4079 381 302 214
BsrBI CCG'CTC
Cuts at: 50 248 3268 3322 4806 4971 50
Size: 198 3020 54 1484 165 55
Fragments arranged by size:
3020 1484 198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2888 2888
Size: 4976
BsrFI r'CCGG_y
Cuts at: 2974 3155 3364 2974
Size: 181 209 4586
Fragments arranged by size:
4586 209 181
BsrGI T'GTAC_A
Cuts at: 1192 1429 1192
Size: 237 4739
BssSI C'ACGA_G
Cuts at: 597 3247 3605 597
Size: 2650 358 1968
Fragments arranged by size:
2650 1968 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2353 2353
Size: 4976
BstBI TT'CG_AA
Cuts at: 119 3337 119
Size: 3218 1758
BstXI CCAn_nnnn'nTGG
Cuts at: 1369 1369
Size: 4976
BstYI r'GATC_y
Cuts at: 1 1095 2001 2468 2491 2826 3072 4084 4170
Size: 1094 906 467 23 335 246 1012 86
Cuts at: 4170 4182 4528 1
Size: 12 346 449
Fragments arranged by size:
1094 1012 906 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 398 107
Size: 291 4685
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2926 3107 3270 2926
Size: 181 163 4632
Fragments arranged by size:
4632 181 163
BtsI GCAGTG_nn'
Cuts at: 1190 1210 1941 2219 1190
Size: 20 731 278 3947
Fragments arranged by size:
3947 731 278 20
DraI TTT'AAA
Cuts at: 1975 1975
Size: 4976
DrdI GACnn_nn'nnGTC
Cuts at: 2682 3540 2682
Size: 858 4118
EaeI y'GGCC_r
Cuts at: 395 2561 2735 3126 3153 4498 395
Size: 2166 174 391 27 1345 873
Fragments arranged by size:
2166 1345 873 391 174 27
EagI C'GGCC_G
Cuts at: 2561 2561
Size: 4976
EarI CTCTTCn'nnn_
Cuts at: 116 238 1541 1542 2999 3209 116
Size: 122 1303 1 1457 210 1883
Fragments arranged by size:
1883 1457 1303 210 122 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1673 3184 3494 3640 4786 127
Size: 1546 1511 310 146 1146 317
Fragments arranged by size:
1546 1511 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1988 1988
Size: 4976
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 902 2801 3233 3980 4248 413
Size: 489 1899 432 747 268 1141
Fragments arranged by size:
1899 1141 747 489 432 268
EcoO109I rG'GnC_Cy
Cuts at: 2115 2115
Size: 4976
EcoRI G'AATT_C
Cuts at: 1980 1980
Size: 4976
EcoRV GAT'ATC
Cuts at: 301 301
Size: 4976
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1102 2995 1102
Size: 1893 3083
FspI TGC'GCA
Cuts at: 2757 2757
Size: 4976
HaeII r_GCGC'y
Cuts at: 232 876 2410 2658 3680 4659 232
Size: 644 1534 248 1022 979 549
Fragments arranged by size:
1534 1022 979 644 549 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 1945 1977 2818 2848 2850 2880 355
Size: 32 1558 32 841 30 2 30 2451
Fragments arranged by size:
2451 1558 841 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2015 2015
Size: 4976
KasI G'GCGC_C
Cuts at: 2406 2654 2406
Size: 248 4728
KpnI G_GTAC'C
Cuts at: 1996 1996
Size: 4976
MluI A'CGCG_T
Cuts at: 4409 4409
Size: 4976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3647 3831 3647
Size: 184 4792
MscI TGG'CCA
Cuts at: 2737 2737
Size: 4976
MslI CAynn'nnrTG
Cuts at: 539 1582 2965 3092 539
Size: 1043 1383 127 2423
Fragments arranged by size:
2423 1383 1043 127
MspA1I CmG'CkG
Cuts at: 922 2087 2401 2761 3774 4019 922
Size: 1165 314 360 1013 245 1879
Fragments arranged by size:
1879 1165 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3157 3157
Size: 4976
NarI GG'CG_CC
Cuts at: 2407 2655 2407
Size: 248 4728
NcoI C'CATG_G
Cuts at: 107 107
Size: 4976
NgoMIV G'CCGG_C
Cuts at: 3155 3155
Size: 4976
NsiI A_TGCA'T
Cuts at: 685 1579 685
Size: 894 4082
NspI r_CATG'y
Cuts at: 1740 2029 2529 3436 1740
Size: 289 500 907 3280
Fragments arranged by size:
3280 907 500 289
PciI A'CATG_T
Cuts at: 3432 3432
Size: 4976
PflMI CCAn_nnn'nTGG
Cuts at: 947 947
Size: 4976
PmeI GTTT'AAAC
Cuts at: 1975 1975
Size: 4976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 4115
Size: 32 4944
PshAI GACnn'nnGTC
Cuts at: 1921 1921
Size: 4976
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1452 1484 1452
Size: 32 4944
PstI C_TGCA'G
Cuts at: 256 1095 1205 2023 2708 256
Size: 839 110 818 685 2524
Fragments arranged by size:
2524 839 818 685 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4976
PvuII CAG'CTG
Cuts at: 922 2401 2761 922
Size: 1479 360 3137
Fragments arranged by size:
3137 1479 360
RsrII CG'GwC_CG
Cuts at: 3171 3171
Size: 4976
SacI G_AGCT'C
Cuts at: 1990 1990
Size: 4976
SalI G'TCGA_C
Cuts at: 2013 2013
Size: 4976
SapI GCTCTTCn'nnn_
Cuts at: 238 1542 2999 3209 238
Size: 1304 1457 210 2005
Fragments arranged by size:
2005 1457 1304 210
SbfI CC_TGCA'GG
Cuts at: 2023 2023
Size: 4976
ScaI AGT'ACT
Cuts at: 1795 1884 4493 1795
Size: 89 2609 2278
Fragments arranged by size:
2609 2278 89
SfcI C'TryA_G
Cuts at: 33 252 380 1091 1201 1478 2019 2704 3697
Size: 219 128 711 110 277 541 685 993
Cuts at: 3697 3888 33
Size: 191 1121
Fragments arranged by size:
1121 993 711 685 541 277 219 191
128 110
SfoI GGC'GCC
Cuts at: 2408 2656 2408
Size: 248 4728
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4976
SmaI CCC'GGG
Cuts at: 1998 1998
Size: 4976
SmlI C'TyrA_G
Cuts at: 770 2131 3046 3538 3800 770
Size: 1361 915 492 262 1946
Fragments arranged by size:
1946 1361 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4976
SphI G_CATG'C
Cuts at: 2029 2529 2029
Size: 500 4476
SspI AAT'ATT
Cuts at: 894 1301 894
Size: 407 4569
StyI C'CwwG_G
Cuts at: 107 2110 2464 107
Size: 2003 354 2619
Fragments arranged by size:
2619 2003 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1407 2559 2692 3151 3360 1407
Size: 1152 133 459 209 3023
Fragments arranged by size:
3023 1152 459 209 133
TatI w'GTAC_w
Cuts at: 1192 1429 1793 1882 4491 1192
Size: 237 364 89 2609 1677
Fragments arranged by size:
2609 1677 364 237 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 183 215 433 465 4493 4525 183
Size: 32 218 32 4028 32 634
Fragments arranged by size:
4028 634 218 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2773 2773
Size: 4976
XbaI T'CTAG_A
Cuts at: 2007 2007
Size: 4976
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2471 2471
Size: 4976
XmaI C'CCGG_G
Cuts at: 1996 1996
Size: 4976
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PshAI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI