################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccIII AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacI SacII ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 709 GACGTC GACGT^C AclI 145 AACGTT AA^CGTT AflIII 296 ACATGT A^C[GA][CT]GT Asp700I 318 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 6 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 575 TGGCCA TGG^CCA BamHI 611 GGATCC G^GATCC BbeI 20 GGCGCC GGCGC^C BglI 1391 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 379 GCTAAGC GC^T[ACGT]AGC Bpu1102I 379 GCTAAGC GC^T[ACGT]AGC BsaBI 642 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 307 GTGCAG GTGCAG(16/14) BsiEI 1393 CGGCCG CG[GA][CT]^CG BsmBI 1525 CGTCTC CGTCTC(1/5) BspHI 167 TCATGA T^CATGA BspLU11I 296 ACATGT A^CATGT BsrDI 86 GCAATG GCAATG(2/0) BssSI 81 CACGAG CACGAG(-5/-1) BstEII 1067 GGTCACC G^GT[ACGT]ACC BstPI 1067 GGTCACC G^GT[ACGT]ACC CelII 379 GCTAAGC GC^T[ACGT]AGC DrdI 1332 GACGCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1393 CGGCCG C^GGCCG EarI 424 GAAGAG CTCTTC(1/4) EclXI 1393 CGGCCG C^GGCCG Eco52I 1393 CGGCCG C^GGCCG EcoO65I 1067 GGTCACC G^GT[ACGT]ACC KasI 20 GGCGCC G^GCGCC Ksp632I 424 GAAGAG CTCTTC(1/4) MamI 642 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 575 TGGCCA TGG^CCA MscI 575 TGGCCA TGG^CCA MslI 168 CATGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 20 GGCGCC GG^CGCC NspI 296 ACATGT [GA]CATG^[CT] PflFI 6 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 145 AACGTT AA^CGTT RcaI 167 TCATGA T^CATGA SapI 424 GAAGAGC GCTCTTC(1/4) SbfI 901 CCTGCAGG CCTGCA^GG SfaNI 1608 GATGC GCATC(5/9) SfoI 20 GGCGCC GGC^GCC SmaI 1614 CCCGGG CCC^GGG Sse8387I 901 CCTGCAGG CCTGCA^GG TfiI 1112 GATTC G^A[AT]TC Tth111I 6 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1614 CCCGGG C^CCGGG XmaI 1614 CCCGGG C^CCGGG XmnI 318 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 3 GTCGAC GT^[AC][GT]AC 1369 GTCGAC AvaI 511 CCCGAG C^[CT]CG[GA]G 1614 CCCGGG BanI 20 GGCGCC G^G[CT][GA]CC 1215 GGTGCC BbsI 1329 GAAGAC GAAGAC(2/6) 712 GTCTTC BbvCI 1013 CCTCAGC CCTCAGC(-5/-2) 1270 GCTGAGG BpuAI 1329 GAAGAC GAAGAC(2/6) 712 GTCTTC BsaAI 331 TACGTG [CT]AC^GT[GA] 1563 CACGTA BsmAI 667 GAGAC GTCTC(1/5) 1526 GTCTC BsoBI 511 CCCGAG C^[CT]CG[GA]G 1614 CCCGGG BsrBI 427 GAGCGG CCGCTC(-3/-3) 607 GAGCGG BstF5I 104 CATCC GGATG(2/0) 624 GGATG FokI 104 CATCC GGATG(9/13) 624 GGATG HaeII 20 GGCGCC [GA]GCGC^[CT] 1554 AGCGCC PflMI 327 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1453 CCAGCCACTGG SalI 3 GTCGAC G^TCGAC 1369 GTCGAC SmlI 15 CTTGAG C^T[CT][GA]AG 442 CTCAAG StuI 889 AGGCCT AGG^CCT 1421 AGGCCT Van91I 327 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1453 CCAGCCACTGG XcmI 230 CCATGACGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 449 CCAAGGAAAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII