Restriction map for
pF1KB5960
(Circular) (Six-base) MAPSORT of: pF1KB5960.seq Check: 8509 from: 1 to: 4766
pF1KB5960 4766 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1188 1410 1188
Size: 222 4544
AatII G_ACGT'C
Cuts at: 821 821
Size: 4766
AccI GT'mk_AC
Cuts at: 112 1478 1804 112
Size: 1366 326 3074
Fragments arranged by size:
3074 1366 326
Acc65I G'GTAC_C
Cuts at: 1782 1782
Size: 4766
AclI AA'CG_TT
Cuts at: 254 4606 254
Size: 4352 414
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 2591 3023 3770 4038 445
Size: 2146 432 747 268 1173
Fragments arranged by size:
2146 1173 747 432 268
AflIII A'CryG_T
Cuts at: 404 2118 3222 4199 404
Size: 1714 1104 977 971
Fragments arranged by size:
1714 1104 977 971
AgeI A'CCGG_T
Cuts at: 3154 3154
Size: 4766
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1288 1288
Size: 4766
AlwNI CAG_nnn'CTG
Cuts at: 423 598 838 946 1051 1567 2071 3638 423
Size: 175 240 108 105 516 504 1567 1551
Fragments arranged by size:
1567 1551 516 504 240 175 108 105
ApaI G_GGCC'C
Cuts at: 211 868 1070 1121 1463 1510 1699 1729 211
Size: 657 202 51 342 47 189 30 3248
Fragments arranged by size:
3248 657 342 202 189 51 47 30
ApaLI G'TGCA_C
Cuts at: 3536 3536
Size: 4766
ApoI r'AATT_y
Cuts at: 1770 4119 4375 1770
Size: 2349 256 2161
Fragments arranged by size:
2349 2161 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4766
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 619 1722 1786 619
Size: 1103 64 3599
Fragments arranged by size:
3599 1103 64
BamHI G'GATC_C
Cuts at: 719 1791 719
Size: 1072 3694
BanI G'GyrC_C
Cuts at: 128 1323 1782 2196 2444 2479 128
Size: 1195 459 414 248 35 2415
Fragments arranged by size:
2415 1195 459 414 248 35
BanII G_rGCy'C
Cuts at: 211 607 868 1070 1121 1235 1463 1510 1699
Size: 396 261 202 51 114 228 47 189
Cuts at: 1699 1729 1780 2810 211
Size: 30 51 1030 2167
Fragments arranged by size:
2167 1030 396 261 228 202 189 114
51 51 47 30
BbeI G_GCGC'C
Cuts at: 132 2200 2448 132
Size: 2068 248 2450
Fragments arranged by size:
2450 2068 248
BbsI GAAGACnn'nnnn_
Cuts at: 813 1444 813
Size: 631 4135
BbvCI CC'TCA_GC
Cuts at: 1122 1379 1122
Size: 257 4509
BciVI GTATCCnnnnn_n'
Cuts at: 2664 3425 4766 2664
Size: 761 1341 2664
Fragments arranged by size:
2664 1341 761
BclI T'GATC_A
Cuts at: 2286 2286
Size: 4766
BglI GCCn_nnn'nGGC
Cuts at: 1505 1505
Size: 4766
BglII A'GATC_T
Cuts at: 2281 4318 2281
Size: 2037 2729
BlpI GC'TnA_GC
Cuts at: 488 1878 488
Size: 1390 3376
Bme1580I G_kGCm'C
Cuts at: 211 868 1070 1121 1463 1510 1699 1729 2391
Size: 657 202 51 342 47 189 30 662
Cuts at: 2391 2484 3540 211
Size: 93 1056 1437
Fragments arranged by size:
1437 1056 662 657 342 202 189 93
51 47 30
BmrI ACTGGGnnnn_n'
Cuts at: 788 1978 2059 2394 4538 788
Size: 1190 81 335 2144 1016
Fragments arranged by size:
2144 1190 1016 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 4766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 923 1014 1324 1541 1679 655
Size: 268 91 310 217 138 3742
Fragments arranged by size:
3742 310 268 217 138 91
Bpu10I CC'TnA_GC
Cuts at: 606 1122 1379 606
Size: 516 257 3993
Fragments arranged by size:
3993 516 257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 535 1942 2821 3313 3611 144
Size: 391 1407 879 492 298 1299
Fragments arranged by size:
1407 1299 879 492 391 298
BsaI GGTCTCn'nnnn_
Cuts at: 4470 4470
Size: 4766
BsaAI yAC'GTr
Cuts at: 441 1673 2119 441
Size: 1232 446 3088
Fragments arranged by size:
3088 1232 446
BsaBI GATnn'nnATC
Cuts at: 6 754 2280 4317 6
Size: 748 1526 2037 455
Fragments arranged by size:
2037 1526 748 455
BsaHI Gr'CG_yC
Cuts at: 129 321 818 1441 2197 2445 3147 4643 129
Size: 192 497 623 756 248 702 1496 252
Fragments arranged by size:
1496 756 702 623 497 252 248 192
BsaWI w'CCGG_w
Cuts at: 1955 2476 3154 3428 3575 3986 1955
Size: 521 678 274 147 411 2735
Fragments arranged by size:
2735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 391 397 421 619 649 781 811 1401
Size: 24 6 24 198 30 132 30 590
Cuts at: 1401 1431 367
Size: 30 3702
Fragments arranged by size:
3702 590 198 132 30 30 30 24
24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 670 673 712 727 775 805 670
Size: 3 39 15 48 30 4631
Fragments arranged by size:
4631 48 39 30 15 3
BseYI C'CCAG_C
Cuts at: 831 867 1044 1057 1189 1560 2191 2949 3526
Size: 36 177 13 132 371 631 758 577
Cuts at: 3526 4022 831
Size: 496 1575
Fragments arranged by size:
1575 758 631 577 496 371 177 132
36 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 436
Size: 4766
BsiEI CG_ry'CG
Cuts at: 104 1504 2308 2354 3562 104
Size: 1400 804 46 1208 1308
Fragments arranged by size:
1400 1308 1208 804 46
BsiHKAI G_wGCw'C
Cuts at: 1780 2558 2748 3540 1780
Size: 778 190 792 3006
Fragments arranged by size:
3006 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1639 1639
Size: 4766
Bsp1286I G_dGCh'C
Cuts at: 211 607 868 1070 1121 1235 1463 1510 1699
Size: 396 261 202 51 114 228 47 189
Cuts at: 1699 1729 1780 2391 2484 2558 2748 2810 3540
Size: 30 51 611 93 74 190 62 730
Cuts at: 3540 211
Size: 1437
Fragments arranged by size:
1437 730 611 396 261 228 202 190
189 114 93 74 62 51 51 47
30
BspEI T'CCGG_A
Cuts at: 3986 3986
Size: 4766
BspHI T'CATG_A
Cuts at: 275 3942 275
Size: 3667 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 746 1188 1410 1816 2030 2332 2713 746
Size: 442 222 406 214 302 381 2799
Fragments arranged by size:
2799 442 406 381 302 222 214
BsrBI CCG'CTC
Cuts at: 50 537 717 3058 3112 4596 4761 50
Size: 487 180 2341 54 1484 165 55
Fragments arranged by size:
2341 1484 487 180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 201 2678 201
Size: 2477 2289
BsrFI r'CCGG_y
Cuts at: 367 521 975 1143 1326 1499 2764 2945 3154
Size: 154 454 168 183 173 1265 181 209
Cuts at: 3154 367
Size: 1979
Fragments arranged by size:
1979 1265 454 209 183 181 173 168
154
BssSI C'ACGA_G
Cuts at: 189 3037 3395 189
Size: 2848 358 1560
Fragments arranged by size:
2848 1560 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2143 2143
Size: 4766
BstBI TT'CG_AA
Cuts at: 3127 3127
Size: 4766
BstEII G'GTnAC_C
Cuts at: 1175 1175
Size: 4766
BstYI r'GATC_y
Cuts at: 1 719 1161 1369 1791 2258 2281 2616 2862
Size: 718 442 208 422 467 23 335 246
Cuts at: 2862 3874 3960 3972 4318 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 718 467 449 442 422 346 335
246 208 86 23 12
BtgI C'CryG_G
Cuts at: 306 581 875 893 1081 306
Size: 275 294 18 188 3991
Fragments arranged by size:
3991 294 275 188 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2716 2897 3060 2716
Size: 181 163 4422
Fragments arranged by size:
4422 181 163
BtsI GCAGTG_nn'
Cuts at: 397 1270 2009 397
Size: 873 739 3154
Fragments arranged by size:
3154 873 739
DraI TTT'AAA
Cuts at: 1765 1765
Size: 4766
DrdI GACnn_nn'nnGTC
Cuts at: 1446 2472 3330 1446
Size: 1026 858 2882
Fragments arranged by size:
2882 1026 858
EaeI y'GGCC_r
Cuts at: 637 683 1501 2351 2525 2916 2943 4288 637
Size: 46 818 850 174 391 27 1345 1115
Fragments arranged by size:
1345 1115 850 818 391 174 46 27
EagI C'GGCC_G
Cuts at: 1501 2351 1501
Size: 850 3916
EarI CTCTTCn'nnn_
Cuts at: 527 2789 2999 527
Size: 2262 210 2294
Fragments arranged by size:
2294 2262 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 181 640 1568 2974 3284 3430 4576 181
Size: 459 928 1406 310 146 1146 371
Fragments arranged by size:
1406 1146 928 459 371 310 146
EcoICRI GAG'CTC
Cuts at: 1778 1778
Size: 4766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 655 923 1014 1324 1541 1679 2591 3023
Size: 210 268 91 310 217 138 912 432
Cuts at: 3023 3770 4038 445
Size: 747 268 1173
Fragments arranged by size:
1173 912 747 432 310 268 268 217
210 138 91
EcoO109I rG'GnC_Cy
Cuts at: 207 610 864 987 1015 1040 1066 1104 1118
Size: 403 254 123 28 25 26 38 14
Cuts at: 1118 1459 1507 1695 1696 1726 1905 207
Size: 341 48 188 1 30 179 3068
Fragments arranged by size:
3068 403 341 254 188 179 123 48
38 30 28 26 25 14 1
EcoRI G'AATT_C
Cuts at: 1770 1770
Size: 4766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4766
FspI TGC'GCA
Cuts at: 2547 2547
Size: 4766
HaeII r_GCGC'y
Cuts at: 132 1666 2200 2448 3470 4449 132
Size: 1534 534 248 1022 979 449
Fragments arranged by size:
1534 1022 979 534 449 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 781 813 1228 1260 1471 1503 2608
Size: 32 382 32 415 32 211 32 1105
Cuts at: 2608 2638 2640 2670 367
Size: 30 2 30 2463
Fragments arranged by size:
2463 1105 415 382 211 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 113 1026 1479 1805 113
Size: 913 453 326 3074
Fragments arranged by size:
3074 913 453 326
KasI G'GCGC_C
Cuts at: 128 2196 2444 128
Size: 2068 248 2450
Fragments arranged by size:
2450 2068 248
KpnI G_GTAC'C
Cuts at: 1786 1786
Size: 4766
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1559 3437 3621 1559
Size: 1878 184 2704
Fragments arranged by size:
2704 1878 184
MscI TGG'CCA
Cuts at: 685 2527 685
Size: 1842 2924
MslI CAynn'nnrTG
Cuts at: 280 2755 2882 280
Size: 2475 127 2164
Fragments arranged by size:
2475 2164 127
MspA1I CmG'CkG
Cuts at: 495 598 650 675 943 1057 1877 2191 2551
Size: 103 52 25 268 114 820 314 360
Cuts at: 2551 3564 3809 495
Size: 1013 245 1452
Fragments arranged by size:
1452 1013 820 360 314 268 245 114
103 52 25
NaeI GCC'GGC
Cuts at: 977 1328 1501 2947 977
Size: 351 173 1446 2796
Fragments arranged by size:
2796 1446 351 173
NarI GG'CG_CC
Cuts at: 129 2197 2445 129
Size: 2068 248 2450
Fragments arranged by size:
2450 2068 248
NcoI C'CATG_G
Cuts at: 306 875 1081 306
Size: 569 206 3991
Fragments arranged by size:
3991 569 206
NgoMIV G'CCGG_C
Cuts at: 975 1326 1499 2945 975
Size: 351 173 1446 2796
Fragments arranged by size:
2796 1446 351 173
NspI r_CATG'y
Cuts at: 408 1819 2319 3226 408
Size: 1411 500 907 1948
Fragments arranged by size:
1948 1411 907 500
PasI CC'CwG_GG
Cuts at: 203 857 917 953 1166 1339 203
Size: 654 60 36 213 173 3630
Fragments arranged by size:
3630 654 213 173 60 36
PciI A'CATG_T
Cuts at: 404 3222 404
Size: 2818 1948
PflMI CCAn_nnn'nTGG
Cuts at: 441 1567 441
Size: 1126 3640
PfoI T'CCnGG_A
Cuts at: 1282 1282
Size: 4766
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4734
PpuMI rG'GwC_Cy
Cuts at: 987 1015 1040 1104 987
Size: 28 25 64 4649
Fragments arranged by size:
4649 64 28 25
PspOMI G'GGCC_C
Cuts at: 207 864 1066 1117 1459 1506 1695 1725 207
Size: 657 202 51 342 47 189 30 3248
Fragments arranged by size:
3248 657 342 202 189 51 47 30
PstI C_TGCA'G
Cuts at: 668 749 1014 1056 1407 1813 2498 668
Size: 81 265 42 351 406 685 2936
Fragments arranged by size:
2936 685 406 351 265 81 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4766
PvuII CAG'CTG
Cuts at: 495 598 675 943 1057 2191 2551 495
Size: 103 77 268 114 1134 360 2710
Fragments arranged by size:
2710 1134 360 268 114 103 77
RsrII CG'GwC_CG
Cuts at: 2961 2961
Size: 4766
SacI G_AGCT'C
Cuts at: 1780 1780
Size: 4766
SalI G'TCGA_C
Cuts at: 111 1477 1803 111
Size: 1366 326 3074
Fragments arranged by size:
3074 1366 326
SanDI GG'GwC_CC
Cuts at: 987 1040 1104 987
Size: 53 64 4649
Fragments arranged by size:
4649 64 53
SapI GCTCTTCn'nnn_
Cuts at: 527 2789 2999 527
Size: 2262 210 2294
Fragments arranged by size:
2294 2262 210
SbfI CC_TGCA'GG
Cuts at: 1014 1813 1014
Size: 799 3967
ScaI AGT'ACT
Cuts at: 4283 4283
Size: 4766
SfcI C'TryA_G
Cuts at: 33 664 745 1010 1052 1403 1809 2494 3487
Size: 631 81 265 42 351 406 685 993
Cuts at: 3487 3678 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 631 406 351 265 191
81 42
SfoI GGC'GCC
Cuts at: 130 2198 2446 130
Size: 2068 248 2450
Fragments arranged by size:
2450 2068 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 1724 1788 1724
Size: 64 4702
SmlI C'TyrA_G
Cuts at: 123 550 1921 2836 3328 3590 123
Size: 427 1371 915 492 262 1299
Fragments arranged by size:
1371 1299 915 492 427 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4766
SphI G_CATG'C
Cuts at: 1819 2319 1819
Size: 500 4266
StuI AGG'CCT
Cuts at: 999 1531 999
Size: 532 4234
StyI C'CwwG_G
Cuts at: 306 557 875 1030 1081 1526 1686 1900 2254
Size: 251 318 155 51 445 160 214 354
Cuts at: 2254 306
Size: 2818
Fragments arranged by size:
2818 445 354 318 251 214 160 155
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 888 1271 1683 2349 2482 2941 3150 888
Size: 383 412 666 133 459 209 2504
Fragments arranged by size:
2504 666 459 412 383 209 133
TatI w'GTAC_w
Cuts at: 4281 4281
Size: 4766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 619 651 1062 1094 1401 1433 4283 4315 619
Size: 32 411 32 307 32 2850 32 1070
Fragments arranged by size:
2850 1070 411 307 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 117 2563 117
Size: 2446 2320
XbaI T'CTAG_A
Cuts at: 1797 1797
Size: 4766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 345 564 2261 345
Size: 219 1697 2850
Fragments arranged by size:
2850 1697 219
XmaI C'CCGG_G
Cuts at: 1722 1786 1722
Size: 64 4702
XmnI GAAnn'nnTTC
Cuts at: 430 430
Size: 4766
ZraI GAC'GTC
Cuts at: 819 819
Size: 4766
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI