Restriction map for pF1KB5955
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AflIII      AgeI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstUI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
PvuII       RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TspRI       
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AclI         1401  AACGTT               AA^CGTT
AcsI         1259  AAATTC               [GA]^AATT[CT]
AhdI          175  GACGACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       382  AGCGCT               AGC^GCT
ApoI         1259  AAATTC               [GA]^AATT[CT]
AspEI         175  GACGACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1348  GAGCTC               G[AT]GC[AT]^C
AvaI          665  CCCGGG               C^[CT]CG[GA]G
BanI          369  GGCACC               G^G[CT][GA]CC
BfaI         1103  CTAG                 C^TAG
BsgI          591  GTGCAG               GTGCAG(16/14)
BsiHKAI      1348  GAGCTC               G[AT]GC[AT]^C
BsoBI         665  CCCGGG               C^[CT]CG[GA]G
BspHI         815  TCATGA               T^CATGA
BspMI        1037  ACCTGC               ACCTGC(4/8)
BsrBI        1012  GAGCGG               CCGCTC(-3/-3)
BsrI           67  CCAGT                ACTGG(1/-1)
BssSI         841  CTCGTG               CACGAG(-5/-1)
BstEII        861  GGTCACC              G^GT[ACGT]ACC
BstPI         861  GGTCACC              G^GT[ACGT]ACC
BstXI         674  CCATCAAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII        1034  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI         1296  GACCGCCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      175  GACGACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      382  AGCGCT               AGC^GCT
EcoNI        1302  CCTGTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1034  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       861  GGTCACC              G^GT[ACGT]ACC
HindIII       426  AAGCTT               A^AGCTT
MaeI         1103  CTAG                 C^TAG
NcoI          637  CCATGG               C^CATGG
PpuMI        1034  GGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I     1401  AACGTT               AA^CGTT
PstI          826  CTGCAG               CTGCA^G
RcaI          815  TCATGA               T^CATGA
SacI         1348  GAGCTC               GAGCT^C
SapI          930  GAAGAGC              GCTCTTC(1/4)
SmaI          665  CCCGGG               CCC^GGG
SmlI           33  CTTGAG               C^T[CT][GA]AG
TaqI         1010  TCGA                 T^CGA
TthHB8I      1010  TCGA                 T^CGA
XmaCI         665  CCCGGG               C^CCGGG
XmaI          665  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AciI         1298  CCGC                 CCGC(-3/-1)
             1014  GCGG                
AlwI          115  GATCC                GGATC(4/5)
              660  GATCC               
AvaII        1035  GGACC                G^G[AT]CC
             1217  GGTCC               
BalI          290  TGGCCA               TGG^CCA
              902  TGGCCA              
BanII         874  GAGCCC               G[GA]GC[CT]^C
             1348  GAGCTC              
BbsI           57  GAAGAC               GAAGAC(2/6)
              819  GAAGAC              
BmyI          874  GAGCCC               G[AGT]GC[ACT]^C
             1348  GAGCTC              
BpuAI          57  GAAGAC               GAAGAC(2/6)
              819  GAAGAC              
BsaI         1395  GAGACC               GGTCTC(1/5)
             1059  GGTCTC              
BseRI         217  GAGGAG               GAGGAG(10/8)
              871  GAGGAG              
Bsp1286I      874  GAGCCC               G[AGT]GC[ACT]^C
             1348  GAGCTC              
BstYI         114  AGATCC               [GA]^GATC[CT]
              659  AGATCC              
CfoI          383  GCGC                 GCG^C
              698  GCGC                
Cfr13I       1035  GGACC                G^G[ACGT]CC
             1217  GGTCC               
DpnII         115  GATC                 ^GATC
              660  GATC                
DsaI          637  CCATGG               C^C[GA][CT]GG
             1445  CCACGG              
EaeI          290  TGGCCA               [CT]^GGCC[GA]
              902  TGGCCA              
HaeII         382  AGCGCT               [GA]GCGC^[CT]
              697  GGCGCT              
HgaI          349  GACGC                GACGC(5/10)
             1421  GCGTC               
HhaI          383  GCGC                 GCG^C
              698  GCGC                
HinP1I        383  GCGC                 G^CGC
              698  GCGC                
MaeII        1289  ACGT                 A^CGT
             1402  ACGT                
MboI          115  GATC                 ^GATC
              660  GATC                
MflI          114  AGATCC               [GA]^GATC[CT]
              659  AGATCC              
MluNI         290  TGGCCA               TGG^CCA
              902  TGGCCA              
MscI          290  TGGCCA               TGG^CCA
              902  TGGCCA              
MslI          463  CATGGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1070  CACTCATGTG          
NdeII         115  GATC                 ^GATC
              660  GATC                
Sau3AI        115  GATC                 ^GATC
              660  GATC                
Sau96I       1035  GGACC                G^G[ACGT]CC
             1217  GGTCC               
SfcI          826  CTGCAG               C^T[GA][CT]AG
             1314  CTACAG              
XcmI          278  CCAGCAACTATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              628  CCACAGAAGCCATGG     
XhoII         114  AGATCC               [GA]^GATC[CT]
              659  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AfaI        AluI        BbvI        BcnI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BssKI       BstF5I      BstNI       Cac8I       DdeI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboII       MnlI        
MseI        MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       PleI        RsaI        ScrFI       
SfaNI       StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     
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