Restriction map for pF1KB5955
 (Linear) (Six-base) MAPSORT of: KIBB5955.seq  Check: 720  from: 1  to: 1473



KIBB5955.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AclI AA'CG_TT

Cuts at:      0    1402    1473 
   Size:       1402      71
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     787     949    1473 
   Size:        787     162     524

  Fragments arranged by size: 

                 787     524     162
 
AfeI AGC'GCT

Cuts at:      0     384    1473 
   Size:        384    1089
 
AhdI GACnn_n'nnGTC

Cuts at:      0     180    1473 
   Size:        180    1293
 
AleI CACnn'nnGTG

Cuts at:      0    1074    1473 
   Size:       1074     399
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     970    1473 
   Size:        970     503
 
ApoI r'AATT_y

Cuts at:      0    1259    1473 
   Size:       1259     214
 
AvaI C'yCGr_G

Cuts at:      0     665    1473 
   Size:        665     808
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     726     759    1473 
   Size:        726      33     714

  Fragments arranged by size: 

                 726     714      33
 
BanI G'GyrC_C

Cuts at:      0     369    1473 
   Size:        369    1104
 
BanII G_rGCy'C

Cuts at:      0     878    1352    1473 
   Size:        878     474     121

  Fragments arranged by size: 

                 878     474     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      64     826    1473 
   Size:         64     762     647

  Fragments arranged by size: 

                 762     647      64
 
BmgBI CAC'GTC

Cuts at:      0    1290    1473 
   Size:       1290     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     407    1154    1473 
   Size:        235     172     747     319

  Fragments arranged by size: 

                 747     319     235     172
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54    1473 
   Size:         54    1419
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1065    1389    1473 
   Size:       1065     324      84

  Fragments arranged by size: 

                1065     324      84
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     122     152    1473 
   Size:        122      30    1321

  Fragments arranged by size: 

                1321     122      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     232     886    1473 
   Size:        232     654     587

  Fragments arranged by size: 

                 654     587     232
 
BseYI C'CCAG_C

Cuts at:      0     277     700    1473 
   Size:        277     423     773

  Fragments arranged by size: 

                 773     423     277
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     612    1473 
   Size:        612     861
 
BsiHKAI G_wGCw'C

Cuts at:      0    1352    1473 
   Size:       1352     121
 
Bsp1286I G_dGCh'C

Cuts at:      0     878    1352    1473 
   Size:        878     474     121

  Fragments arranged by size: 

                 878     474     121
 
BspHI T'CATG_A

Cuts at:      0     815    1473 
   Size:        815     658
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1046    1473 
   Size:       1046     427
 
BsrBI CCG'CTC

Cuts at:      0    1014    1473 
   Size:       1014     459
 
BssSI C'ACGA_G

Cuts at:      0     841    1473 
   Size:        841     632
 
BstEII G'GTnAC_C

Cuts at:      0     861    1473 
   Size:        861     612
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     681    1473 
   Size:        681     792
 
BstYI r'GATC_y

Cuts at:      0     114     659    1473 
   Size:        114     545     814

  Fragments arranged by size: 

                 814     545     114
 
BtgI C'CryG_G

Cuts at:      0     637    1445    1473 
   Size:        637     808      28

  Fragments arranged by size: 

                 808     637      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     950    1473 
   Size:        950     523
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1302    1473 
   Size:       1302     171
 
EaeI y'GGCC_r

Cuts at:      0     290     902    1473 
   Size:        290     612     571

  Fragments arranged by size: 

                 612     571     290
 
EarI CTCTTCn'nnn_

Cuts at:      0     311     925    1385    1473 
   Size:        311     614     460      88

  Fragments arranged by size: 

                 614     460     311      88
 
EcoICRI GAG'CTC

Cuts at:      0    1350    1473 
   Size:       1350     123
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     407     787     949    1154    1473 
   Size:        235     172     380     162     205     319

  Fragments arranged by size: 

                 380     319     235     205     172     162
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1306    1473 
   Size:       1306     167
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1035    1473 
   Size:       1035     438
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     973    1473 
   Size:        973     500
 
HaeII r_GCGC'y

Cuts at:      0     386     701    1473 
   Size:        386     315     772

  Fragments arranged by size: 

                 772     386     315
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198    1201    1233    1473 
   Size:        166      32    1003      32     240

  Fragments arranged by size: 

                1003     240     166      32      32
 
HindIII A'AGCT_T

Cuts at:      0     426    1473 
   Size:        426    1047
 
MscI TGG'CCA

Cuts at:      0     292     904    1473 
   Size:        292     612     569

  Fragments arranged by size: 

                 612     569     292
 
MslI CAynn'nnrTG

Cuts at:      0     467    1074    1473 
   Size:        467     607     399

  Fragments arranged by size: 

                 607     467     399
 
NcoI C'CATG_G

Cuts at:      0     637    1473 
   Size:        637     836
 
PasI CC'CwG_GG

Cuts at:      0    1270    1308    1473 
   Size:       1270      38     165

  Fragments arranged by size: 

                1270     165      38
 
PfoI T'CCnGG_A

Cuts at:      0     212     797    1424    1473 
   Size:        212     585     627      49

  Fragments arranged by size: 

                 627     585     212      49
 
PpuMI rG'GwC_Cy

Cuts at:      0    1035    1473 
   Size:       1035     438
 
PstI C_TGCA'G

Cuts at:      0     830    1473 
   Size:        830     643
 
SacI G_AGCT'C

Cuts at:      0    1352    1473 
   Size:       1352     121
 
SapI GCTCTTCn'nnn_

Cuts at:      0     925    1473 
   Size:        925     548
 
SfcI C'TryA_G

Cuts at:      0     826    1314    1473 
   Size:        826     488     159

  Fragments arranged by size: 

                 826     488     159
 
SmaI CCC'GGG

Cuts at:      0     667    1473 
   Size:        667     806
 
SmlI C'TyrA_G

Cuts at:      0      33    1473 
   Size:         33    1440
 
StyI C'CwwG_G

Cuts at:      0     637     746     905    1473 
   Size:        637     109     159     568

  Fragments arranged by size: 

                 637     568     159     109
 
TatI w'GTAC_w

Cuts at:      0     896    1124    1473 
   Size:        896     228     349

  Fragments arranged by size: 

                 896     349     228
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     122     154    1311    1343    1473 
   Size:        122      32    1157      32     130

  Fragments arranged by size: 

                1157     130     122      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     285     635    1473 
   Size:        285     350     838

  Fragments arranged by size: 

                 838     350     285
 
XmaI C'CCGG_G

Cuts at:      0     665    1473 
   Size:        665     808
 

 Enzymes that do cut:

     AclI     AcuI     AfeI     AhdI     AleI     AlfI     ApoI     AvaI
     BaeI     BanI    BanII     BbsI    BmgBI     BpmI    BpuEI     BsaI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I  HindIII     MscI     MslI     NcoI     PasI     PfoI    PpuMI
     PstI     SacI     SapI     SfcI     SmaI     SmlI     StyI     TatI
     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I    AflII   AflIII     AgeI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmnI
     ZraI
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