Restriction map for pF1KB5940
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AclI        AflIII      AhdI        
Alw44I      AlwNI       AocI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbvCI       BciVI       BclI        BglI        BlnI        
BpmI        BsaAI       BsaBI       BseRI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       Bsu36I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoRV       FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MunI        NheI        NotI        NruI        NspV        
PacI        PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI          334  GTATAC               GT^[AC][GT]AC
AflII        1018  CTTAAG               C^TTAAG
AgeI         1301  ACCGGT               A^CCGGT
AlwI          411  GATCC                GGATC(4/5)
Aor51HI       144  AGCGCT               AGC^GCT
ApaI          943  GGGCCC               GGGCC^C
BalI         1515  TGGCCA               TGG^CCA
BanI          117  GGCGCC               G^G[CT][GA]CC
BbeI          117  GGCGCC               GGCGC^C
BbsI          493  GAAGAC               GAAGAC(2/6)
BcgI         1007  CGATTAGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1018  CTTAAG               C^TTAAG
BlpI         1345  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1345  GCTCAGC              GC^T[ACGT]AGC
BpuAI         493  GAAGAC               GAAGAC(2/6)
BsaI          950  GAGACC               GGTCTC(1/5)
BsmAI         950  GAGAC                GTCTC(1/5)
BspMI         461  ACCTGC               ACCTGC(4/8)
BsrDI        1132  GCAATG               GCAATG(2/0)
Bst1107I      334  GTATAC               GTA^TAC
BstEII        999  GGTGACC              G^GT[ACGT]ACC
BstPI         999  GGTGACC              G^GT[ACGT]ACC
BstZ17I       334  GTATAC               GTA^TAC
CelII        1345  GCTCAGC              GC^T[ACGT]AGC
DraI          589  TTTAAA               TTT^AAA
Eco47III      144  AGCGCT               AGC^GCT
EcoNI         466  CCTCACATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       999  GGTGACC              G^GT[ACGT]ACC
EcoRI        1119  GAATTC               G^AATTC
EcoT14I      1312  CCATGG               C^C[AT][AT]GG
EcoT22I      1051  ATGCAT               ATGCA^T
HgaI          103  GACGC                GACGC(5/10)
KasI          117  GGCGCC               G^GCGCC
MaeII         991  ACGT                 A^CGT
MluNI        1515  TGGCCA               TGG^CCA
MscI         1515  TGGCCA               TGG^CCA
NaeI          120  GCCGGC               GCC^GGC
NarI          117  GGCGCC               GG^CGCC
NcoI         1312  CCATGG               C^CATGG
NdeI          619  CATATG               CA^TATG
NgoMIV        120  GCCGGC               G^CCGGC
NsiI         1051  ATGCAT               ATGCA^T
PaeR7I        389  CTCGAG               C^TCGAG
PinAI        1301  ACCGGT               A^CCGGT
PpuMI         166  GGGACCC              [GA]G^G[AT]CC[CT]
SacI         1275  GAGCTC               GAGCT^C
SapI          976  GAAGAGC              GCTCTTC(1/4)
SfaNI        1053  GCATC                GCATC(5/9)
SfoI          117  GGCGCC               GGC^GCC
SmaI           50  CCCGGG               CCC^GGG
SphI         1028  GCATGC               GCATG^C
StyI         1312  CCATGG               C^C[AT][AT]GG
TaqI          390  TCGA                 T^CGA
TthHB8I       390  TCGA                 T^CGA
XbaI         1333  TCTAGA               T^CTAGA
XhoI          389  CTCGAG               C^TCGAG
XmaCI          50  CCCGGG               C^CCGGG
XmaI           50  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccIII        159  TCCGGA               T^CCGGA
              216  TCCGGA              
AcyI          103  GACGCC               G[GA]^CG[CT]C
              117  GGCGCC              
BanII         943  GGGCCC               G[GA]GC[CT]^C
             1275  GAGCTC              
BcnI           50  CCCGG                CC^[GC]GG
               51  CCGGG               
BfaI         1334  CTAG                 C^TAG
             1607  CTAG                
BsaHI         103  GACGCC               G[GA]^CG[CT]C
              117  GGCGCC              
BseAI         159  TCCGGA               T^CCGGA
              216  TCCGGA              
BsgI          133  CTGCAC               GTGCAG(16/14)
              968  GTGCAG              
BsmFI         166  GGGAC                GGGAC(10/14)
             1305  GTCCC               
BspEI         159  TCCGGA               T^CCGGA
              216  TCCGGA              
BsrFI         120  GCCGGC               [GA]^CCGG[CT]
             1301  ACCGGT              
BstXI          72  CCAGGTGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1283  CCACAAACATGG        
Cfr10I        120  GCCGGC               [GA]^CCGG[CT]
             1301  ACCGGT              
EaeI           65  TGGCCG               [CT]^GGCC[GA]
             1515  TGGCCA              
EarI         1266  CTCTTC               CTCTTC(1/4)
              976  GAAGAG              
FauI          170  CCCGC                CCCGC(4/6)
              937  GCGGG               
Hin1I         103  GACGCC               G[GA]^CG[CT]C
              117  GGCGCC              
Ksp632I      1266  CTCTTC               CTCTTC(1/4)
              976  GAAGAG              
KspI          171  CCGCGG               CCGC^GG
              820  CCGCGG              
MaeI         1334  CTAG                 C^TAG
             1607  CTAG                
MroI          159  TCCGGA               T^CCGGA
              216  TCCGGA              
NciI           50  CCCGG                CC^[GC]GG
               51  CCGGG               
NspI          723  ACATGC               [GA]CATG^[CT]
             1028  GCATGC              
PleI         1208  GACTC                GAGTC(4/5)
             1437  GAGTC               
PstI          182  CTGCAG               CTGCA^G
             1077  CTGCAG              
SacII         171  CCGCGG               CCGC^GG
              820  CCGCGG              
SfcI          182  CTGCAG               C^T[GA][CT]AG
             1077  CTGCAG              
SmlI          389  CTCGAG               C^T[CT][GA]AG
             1018  CTTAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
ApoI        AspHI       AvaI        AvaII       BbvI        
BglII       BmyI        BsaJI       BsaWI       BsiHKAI     
BsiYI       BslI        BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I      XhoII       
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