Restriction map for pF1KB5909
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflIII      AgeI        AhdI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvrII       BbeI        
BcgI        BlnI        BpmI        BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BspHI       BspLU11I    BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FseI        HaeII       HgaI        
Hin1I       HincII      HindII      HpaI        KasI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tsp509I     Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AflII        1270  CTTAAG               C^TTAAG
Alw44I       1064  GTGCAC               G^TGCAC
AocI          583  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1064  GTGCAC               G^TGCAC
AviII        1262  TGCGCA               TGC^GCA
BamHI         668  GGATCC               G^GATCC
BbrPI         637  CACGTG               CAC^GTG
BbsI          573  GAAGAC               GAAGAC(2/6)
BciVI          49  GTATCC               GTATCC(6/5)
BclI          978  TGATCA               T^GATCA
BcnI          213  CCCGG                CC^[GC]GG
BfaI         1187  CTAG                 C^TAG
BfrI         1270  CTTAAG               C^TTAAG
BglI         1169  GCCACTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          609  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      609  GCTCAGC              GC^T[ACGT]AGC
BpuAI         573  GAAGAC               GAAGAC(2/6)
BsaAI         637  CACGTG               [CT]AC^GT[GA]
BsaI         1039  GAGACC               GGTCTC(1/5)
BsgI          259  CTGCAC               GTGCAG(16/14)
BsmBI         355  CGTCTC               CGTCTC(1/5)
BsmI          659  GCATTC               GAATGC(1/-1)
Bsp1407I      894  TGTACA               T^GTACA
BspMI         624  GCAGGT               ACCTGC(4/8)
BsrBI          76  GAGCGG               CCGCTC(-3/-3)
BsrFI         620  ACCGGC               [GA]^CCGG[CT]
BsrGI         894  TGTACA               T^GTACA
BstEII        798  GGTCACC              G^GT[ACGT]ACC
BstPI         798  GGTCACC              G^GT[ACGT]ACC
BstXI        1433  CCACAGGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        583  CCTGAGG              CC^T[ACGT]AGG
CelII         609  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        620  ACCGGC               [GA]^CCGG[CT]
DrdI         1237  GACTCCTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        583  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       798  GGTCACC              G^GT[ACGT]ACC
FbaI          978  TGATCA               T^GATCA
FspI         1262  TGCGCA               TGC^GCA
MaeI         1187  CTAG                 C^TAG
NciI          213  CCCGG                CC^[GC]GG
NcoI         1106  CCATGG               C^CATGG
PmaCI         637  CACGTG               CAC^GTG
PmlI          637  CACGTG               CAC^GTG
PpuMI         827  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          286  CTGCAG               CTGCA^G
SapI          604  GAAGAGC              GCTCTTC(1/4)
ScaI          983  AGTACT               AGT^ACT
SexAI         830  ACCTGGT              A^CC[AT]GGT
SfiI         1168  GGCCACTCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI         894  TGTACA               T^GTACA
StuI          930  AGGCCT               AGG^CCT
TfiI         1401  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

Acc65I        112  GGTACC               G^GTACC
             1339  GGTACC              
ApaI          908  GGGCCC               GGGCC^C
             1176  GGGCCC              
Asp718I       112  GGTACC               G^GTACC
             1339  GGTACC              
AvaI          380  CCCGAG               C^[CT]CG[GA]G
             1173  CTCGGG              
BalI         1154  TGGCCA               TGG^CCA
             1167  TGGCCA              
BanI          112  GGTACC               G^G[CT][GA]CC
             1339  GGTACC              
BbvCI         197  CCTCAGC              CCTCAGC(-5/-2)
              933  CCTCAGC             
BglII        1124  AGATCT               A^GATCT
             1373  AGATCT              
BsaBI        1119  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1374  GATCTACATC          
BsoBI         380  CCCGAG               C^[CT]CG[GA]G
             1173  CTCGGG              
BsrDI          99  CATTGC               GCAATG(2/0)
              348  CATTGC              
DraII         827  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1176  GGGCCCC             
DsaI          551  CCACGG               C^C[GA][CT]GG
             1106  CCATGG              
EarI          564  GAAGAG               CTCTTC(1/4)
              604  GAAGAG              
EcoO109I      827  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1176  GGGCCCC             
FauI          296  CCCGC                CCCGC(4/6)
              466  GCGGG               
KpnI          112  GGTACC               GGTAC^C
             1339  GGTACC              
Ksp632I       564  GAAGAG               CTCTTC(1/4)
              604  GAAGAG              
MaeII         638  ACGT                 A^CGT
             1143  ACGT                
MamI         1119  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1374  GATCTACATC          
MluNI        1154  TGGCCA               TGG^CCA
             1167  TGGCCA              
MscI         1154  TGGCCA               TGG^CCA
             1167  TGGCCA              
MseI         1271  TTAA                 T^TAA
             1280  TTAA                
PflMI         406  CCACAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              825  CCAGGACCTGG         
PvuII         283  CAGCTG               CAG^CTG
             1291  CAGCTG              
SacI          607  GAGCTC               GAGCT^C
              966  GAGCTC              
SmlI         1270  CTTAAG               C^T[CT][GA]AG
             1495  CTCAAG              
TaqI           89  TCGA                 T^CGA
              158  TCGA                
Tru9I        1271  TTAA                 T^TAA
             1280  TTAA                
TthHB8I        89  TCGA                 T^CGA
              158  TCGA                
Van91I        406  CCACAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              825  CCAGGACCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BanII       BbvI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TseI        Tsp45I      
TspRI       XhoII       
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