Restriction map for pF1KB5909
 (Circular) (Six-base) MAPSORT of: pF1KB5909.seq  Check: 1664  from: 1  to: 4625



pF1KB5909  4625 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    723     723 
   Size:       4625
 
AccI GT'mk_AC

Cuts at:   1663    1663 
   Size:       4625
 
Acc65I G'GTAC_C

Cuts at:    220    1447    1641     220 
   Size:       1227     194    3204

  Fragments arranged by size: 

                3204    1227     194
 
AclI AA'CG_TT

Cuts at:   4465    4465 
   Size:       4625
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2450    2882    3629    3897    2450 
   Size:        432     747     268    3178

  Fragments arranged by size: 

                3178     747     432     268
 
AflII C'TTAA_G

Cuts at:   1378    1378 
   Size:       4625
 
AflIII A'CryG_T

Cuts at:   1977    3081    4058    1977 
   Size:       1104     977    2544

  Fragments arranged by size: 

                2544    1104     977
 
AgeI A'CCGG_T

Cuts at:   3013    3013 
   Size:       4625
 
AleI CACnn'nnGTG

Cuts at:    152     913    1135    1546     152 
   Size:        761     222     411    3231

  Fragments arranged by size: 

                3231     761     411     222
 
AlwNI CAG_nnn'CTG

Cuts at:    393     939    1339    1930    3497     393 
   Size:        546     400     591    1567    1521

  Fragments arranged by size: 

                1567    1521     591     546     400
 
ApaI G_GGCC'C

Cuts at:   1020    1288    1020 
   Size:        268    4357
 
ApaLI G'TGCA_C

Cuts at:   1172    3395    1172 
   Size:       2223    2402
 
ApoI r'AATT_y

Cuts at:   1629    3978    4234    1629 
   Size:       2349     256    2020

  Fragments arranged by size: 

                2349    2020     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4625
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4625
 
AvaI C'yCGr_G

Cuts at:    488    1281    1645     488 
   Size:        793     364    3468

  Fragments arranged by size: 

                3468     793     364
 
BamHI G'GATC_C

Cuts at:    776    1650     776 
   Size:        874    3751
 
BanI G'GyrC_C

Cuts at:    220    1447    1641    2055    2303    2338     220 
   Size:       1227     194     414     248      35    2507

  Fragments arranged by size: 

                2507    1227     414     248     194      35
 
BanII G_rGCy'C

Cuts at:    719    1020    1078    1288    1639    2669     719 
   Size:        301      58     210     351    1030    2675

  Fragments arranged by size: 

                2675    1030     351     301     210      58
 
BbeI G_GCGC'C

Cuts at:   2059    2307    2059 
   Size:        248    4377
 
BbsI GAAGACnn'nnnn_

Cuts at:    688     688 
   Size:       4625
 
BbvCI CC'TCA_GC

Cuts at:    306    1042     306 
   Size:        736    3889
 
BciVI GTATCCnnnnn_n'

Cuts at:    168    2523    3284    4625     168 
   Size:       2355     761    1341     168

  Fragments arranged by size: 

                2355    1341     761     168
 
BclI T'GATC_A

Cuts at:   1086    2145    1086 
   Size:       1059    3566
 
BglI GCCn_nnn'nGGC

Cuts at:   1283    1283 
   Size:       4625
 
BglII A'GATC_T

Cuts at:   1232    1481    2140    4177    1232 
   Size:        249     659    2037    1680

  Fragments arranged by size: 

                2037    1680     659     249
 
BlpI GC'TnA_GC

Cuts at:    718    1737     718 
   Size:       1019    3606
 
Bme1580I G_kGCm'C

Cuts at:    536    1020    1176    1288    1471    2250    2343    3399     536 
   Size:        484     156     112     183     779      93    1056    1762

  Fragments arranged by size: 

                1762    1056     779     484     183     156     112      93
            
 
BmrI ACTGGGnnnn_n'

Cuts at:   1104    1837    1918    2253    4397    1104 
   Size:        733      81     335    2144    1332

  Fragments arranged by size: 

                2144    1332     733     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2992    2992 
   Size:       4625
 
Bpu10I CC'TnA_GC

Cuts at:    306    1042    1072     306 
   Size:        736      30    3859

  Fragments arranged by size: 

                3859     736      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1588    1801    2680    3172    3470    1588 
   Size:        213     879     492     298    2743

  Fragments arranged by size: 

                2743     879     492     298     213
 
BsaI GGTCTCn'nnnn_

Cuts at:   1141    4329    1141 
   Size:       3188    1437
 
BsaAI yAC'GTr

Cuts at:    747    1978     747 
   Size:       1231    3394
 
BsaBI GATnn'nnATC

Cuts at:      6    1231    1486    2139    4176       6 
   Size:       1225     255     653    2037     455

  Fragments arranged by size: 

                2037    1225     653     455     255
 
BsaHI Gr'CG_yC

Cuts at:   2056    2304    3006    4502    2056 
   Size:        248     702    1496    2179

  Fragments arranged by size: 

                2179    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1814    2335    3013    3287    3434    3845    1814 
   Size:        521     678     274     147     411    2594

  Fragments arranged by size: 

                2594     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    268     709    1068    1338     268 
   Size:        441     359     270    3555

  Fragments arranged by size: 

                3555     441     359     270
 
BseYI C'CCAG_C

Cuts at:   2050    2808    3385    3881    2050 
   Size:        758     577     496    2794

  Fragments arranged by size: 

                2794     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    352     352 
   Size:       4625
 
BsiEI CG_ry'CG

Cuts at:    104    2167    2213    3421     104 
   Size:       2063      46    1208    1308

  Fragments arranged by size: 

                2063    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    149     719    1078    1130    1176    1639    2417    2607    3399 
   Size:        570     359      52      46     463     778     190     792

Cuts at:   3399     149 
   Size:       1375

  Fragments arranged by size: 

                1375     792     778     570     463     359     190      52
                  46
 
BsmI GAATG_Cn'

Cuts at:    767     767 
   Size:       4625
 
BsmBI CGTCTCn'nnnn_

Cuts at:    469     469 
   Size:       4625
 
Bsp1286I G_dGCh'C

Cuts at:    149     536     719    1020    1078    1130    1176    1288    1471 
   Size:        387     183     301      58      52      46     112     183

Cuts at:   1471    1639    2250    2343    2417    2607    2669    3399     149 
   Size:        168     611      93      74     190      62     730    1375

  Fragments arranged by size: 

                1375     730     611     387     301     190     183     183
                 168     112      93      74      62      58      52      46
            
 
BspEI T'CCGG_A

Cuts at:   3845    3845 
   Size:       4625
 
BspHI T'CATG_A

Cuts at:   3801    3801 
   Size:       4625
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    723    1675    1889    2191    2572     723 
   Size:        952     214     302     381    2776

  Fragments arranged by size: 

                2776     952     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     186    2917    2971    4455    4620      50 
   Size:        136    2731      54    1484     165      55

  Fragments arranged by size: 

                2731    1484     165     136      55      54
 
BsrDI GCAATG_nn'

Cuts at:    206     455    2537     206 
   Size:        249    2082    2294

  Fragments arranged by size: 

                2294    2082     249
 
BsrFI r'CCGG_y

Cuts at:    728    2623    2804    3013     728 
   Size:       1895     181     209    2340

  Fragments arranged by size: 

                2340    1895     209     181
 
BsrGI T'GTAC_A

Cuts at:   1002    1002 
   Size:       4625
 
BssSI C'ACGA_G

Cuts at:   2896    3254    2896 
   Size:        358    4267
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2002    2002 
   Size:       4625
 
BstBI TT'CG_AA

Cuts at:   2986    2986 
   Size:       4625
 
BstEII G'GTnAC_C

Cuts at:    906     906 
   Size:       4625
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1548    1548 
   Size:       4625
 
BstYI r'GATC_y

Cuts at:      1     776     815    1232    1365    1481    1650    2117    2140 
   Size:        775      39     417     133     116     169     467      23

Cuts at:   2140    2475    2721    3733    3819    3831    4177       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     775     467     449     417     346     335     246
                 169     133     116      86      39      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    692     692 
   Size:       4625
 
BtgI C'CryG_G

Cuts at:    659    1214     659 
   Size:        555    4070
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1284    1560    2575    2756    2919    1284 
   Size:        276    1015     181     163    2990

  Fragments arranged by size: 

                2990    1015     276     181     163
 
BtsI GCAGTG_nn'

Cuts at:    479    1868     479 
   Size:       1389    3236
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    548     583     548 
   Size:         35    4590
 
DraI TTT'AAA

Cuts at:   1624    1624 
   Size:       4625
 
DrdI GACnn_nn'nnGTC

Cuts at:   1351    2331    3189    1351 
   Size:        980     858    2787

  Fragments arranged by size: 

                2787     980     858
 
EaeI y'GGCC_r

Cuts at:    323    1262    1275    2210    2384    2775    2802    4147     323 
   Size:        939      13     935     174     391      27    1345     801

  Fragments arranged by size: 

                1345     939     935     801     391     174      27      13
            
 
EagI C'GGCC_G

Cuts at:   2210    2210 
   Size:       4625
 
EarI CTCTTCn'nnn_

Cuts at:    667     707    2648    2858     667 
   Size:         40    1941     210    2434

  Fragments arranged by size: 

                2434    1941     210      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    847    2833    3143    3289    4435     847 
   Size:       1986     310     146    1146    1037

  Fragments arranged by size: 

                1986    1146    1037     310     146
 
EcoICRI GAG'CTC

Cuts at:    717    1076    1637     717 
   Size:        359     561    3705

  Fragments arranged by size: 

                3705     561     359
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   2450    2882    3629    3897    2450 
   Size:        432     747     268    3178

  Fragments arranged by size: 

                3178     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    936    1285    1764     936 
   Size:        349     479    3797

  Fragments arranged by size: 

                3797     479     349
 
EcoRI G'AATT_C

Cuts at:   1629    1629 
   Size:       4625
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2644    2644 
   Size:       4625
 
FspI TGC'GCA

Cuts at:   1372    2406    1372 
   Size:       1034    3591
 
HaeII r_GCGC'y

Cuts at:   2059    2307    3329    4308    2059 
   Size:        248    1022     979    2376

  Fragments arranged by size: 

                2376    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1135    1167    1333    1365    1408    1440    2467    2497    2499 
   Size:         32     166      32      43      32    1027      30       2

Cuts at:   2499    2529    1135 
   Size:         30    3231

  Fragments arranged by size: 

                3231    1027     166      43      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1664    1664 
   Size:       4625
 
HindIII A'AGCT_T

Cuts at:    335     631    1375     335 
   Size:        296     744    3585

  Fragments arranged by size: 

                3585     744     296
 
KasI G'GCGC_C

Cuts at:   2055    2303    2055 
   Size:        248    4377
 
KpnI G_GTAC'C

Cuts at:    224    1451    1645     224 
   Size:       1227     194    3204

  Fragments arranged by size: 

                3204    1227     194
 
MluI A'CGCG_T

Cuts at:   4058    4058 
   Size:       4625
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    827    3296    3480     827 
   Size:       2469     184    1972

  Fragments arranged by size: 

                2469    1972     184
 
MscI TGG'CCA

Cuts at:   1264    1277    2386    1264 
   Size:         13    1109    3503

  Fragments arranged by size: 

                3503    1109      13
 
MslI CAynn'nnrTG

Cuts at:    152     179     913    1025    1135    1546    2614    2741     152 
   Size:         27     734     112     110     411    1068     127    2036

  Fragments arranged by size: 

                2036    1068     734     411     127     112     110      27
            
 
MspA1I CmG'CkG

Cuts at:    393    1336    1401    1736    2050    2410    3423    3668     393 
   Size:        943      65     335     314     360    1013     245    1350

  Fragments arranged by size: 

                1350    1013     943     360     335     314     245      65
            
 
NaeI GCC'GGC

Cuts at:   2806    2806 
   Size:       4625
 
NarI GG'CG_CC

Cuts at:   2056    2304    2056 
   Size:        248    4377
 
NcoI C'CATG_G

Cuts at:   1214    1214 
   Size:       4625
 
NgoMIV G'CCGG_C

Cuts at:   2804    2804 
   Size:       4625
 
NspI r_CATG'y

Cuts at:   1678    2178    3085    1678 
   Size:        500     907    3218

  Fragments arranged by size: 

                3218     907     500
 
PciI A'CATG_T

Cuts at:   3081    3081 
   Size:       4625
 
PflMI CCAn_nnn'nTGG

Cuts at:    520     939     520 
   Size:        419    4206
 
PmeI GTTT'AAAC

Cuts at:   1624    1624 
   Size:       4625
 
PmlI CAC'GTG

Cuts at:    747     747 
   Size:       4625
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3764    3796    3764 
   Size:         32    4593
 
PpuMI rG'GwC_Cy

Cuts at:    936     936 
   Size:       4625
 
PspOMI G'GGCC_C

Cuts at:   1016    1284    1016 
   Size:        268    4357
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    995    1027     995 
   Size:         32    4593
 
PstI C_TGCA'G

Cuts at:    398    1672    2357     398 
   Size:       1274     685    2666

  Fragments arranged by size: 

                2666    1274     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4625
 
PvuII CAG'CTG

Cuts at:    393    1401    2050    2410     393 
   Size:       1008     649     360    2608

  Fragments arranged by size: 

                2608    1008     649     360
 
RsrII CG'GwC_CG

Cuts at:   2820    2820 
   Size:       4625
 
SacI G_AGCT'C

Cuts at:    719    1078    1639     719 
   Size:        359     561    3705

  Fragments arranged by size: 

                3705     561     359
 
SalI G'TCGA_C

Cuts at:   1662    1662 
   Size:       4625
 
SapI GCTCTTCn'nnn_

Cuts at:    707    2648    2858     707 
   Size:       1941     210    2474

  Fragments arranged by size: 

                2474    1941     210
 
SbfI CC_TGCA'GG

Cuts at:   1672    1672 
   Size:       4625
 
ScaI AGT'ACT

Cuts at:   1093    4142    1093 
   Size:       3049    1576
 
SexAI A'CCwGG_T

Cuts at:    938     938 
   Size:       4625
 
SfcI C'TryA_G

Cuts at:     33     394    1331    1611    1668    2353    3346    3537      33 
   Size:        361     937     280      57     685     993     191    1121

  Fragments arranged by size: 

                1121     993     937     685     361     280     191      57
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1283    1283 
   Size:       4625
 
SfoI GGC'GCC

Cuts at:   2057    2305    2057 
   Size:        248    4377
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4625
 
SmaI CCC'GGG

Cuts at:   1647    1647 
   Size:       4625
 
SmlI C'TyrA_G

Cuts at:   1378    1603    1780    2695    3187    3449    1378 
   Size:        225     177     915     492     262    2554

  Fragments arranged by size: 

                2554     915     492     262     225     177
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4625
 
SphI G_CATG'C

Cuts at:   1678    2178    1678 
   Size:        500    4125
 
StuI AGG'CCT

Cuts at:   1040    1040 
   Size:       4625
 
StyI C'CwwG_G

Cuts at:    470    1133    1214    1759    2113     470 
   Size:        663      81     545     354    2982

  Fragments arranged by size: 

                2982     663     545     354      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    164     299     559     583     972    2208    2341    2800    3009 
   Size:        135     260      24     389    1236     133     459     209

Cuts at:   3009     164 
   Size:       1780

  Fragments arranged by size: 

                1780    1236     459     389     260     209     135     133
                  24
 
TatI w'GTAC_w

Cuts at:    203    1002    1091    4140     203 
   Size:        799      89    3049     688

  Fragments arranged by size: 

                3049     799     688      89
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4142    4174    4142 
   Size:         32    4593
 
Tth111I GACn'n_nGTC

Cuts at:   2422    2422 
   Size:       4625
 
XbaI T'CTAG_A

Cuts at:   1656    1656 
   Size:       4625
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2120    2120 
   Size:       4625
 
XmaI C'CCGG_G

Cuts at:   1645    1645 
   Size:       4625
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AlfI     AloI     AscI    AvrII     BaeI
     BcgI    BfrBI    BmgBI     BmtI     BpmI    BsaXI    BsiWI   BssHII
  BstZ17I     ClaI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI    PshAI     PsiI    PspXI    SacII    SanDI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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