Restriction map for pF1KB5909
 (Linear) (Six-base) MAPSORT of: KIBB5909.seq  Check: 1504  from: 1  to: 1512



KIBB5909.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     615    1512 
   Size:        615     897
 
Acc65I G'GTAC_C

Cuts at:      0     112    1339    1512 
   Size:        112    1227     173

  Fragments arranged by size: 

                1227     173     112
 
AflII C'TTAA_G

Cuts at:      0    1270    1512 
   Size:       1270     242
 
AleI CACnn'nnGTG

Cuts at:      0      44     805    1027    1438    1512 
   Size:         44     761     222     411      74

  Fragments arranged by size: 

                 761     411     222      74      44
 
AlwNI CAG_nnn'CTG

Cuts at:      0     285     831    1231    1512 
   Size:        285     546     400     281

  Fragments arranged by size: 

                 546     400     285     281
 
ApaI G_GGCC'C

Cuts at:      0     912    1180    1512 
   Size:        912     268     332

  Fragments arranged by size: 

                 912     332     268
 
ApaLI G'TGCA_C

Cuts at:      0    1064    1512 
   Size:       1064     448
 
AvaI C'yCGr_G

Cuts at:      0     380    1173    1512 
   Size:        380     793     339

  Fragments arranged by size: 

                 793     380     339
 
BamHI G'GATC_C

Cuts at:      0     668    1512 
   Size:        668     844
 
BanI G'GyrC_C

Cuts at:      0     112    1339    1512 
   Size:        112    1227     173

  Fragments arranged by size: 

                1227     173     112
 
BanII G_rGCy'C

Cuts at:      0     611     912     970    1180    1512 
   Size:        611     301      58     210     332

  Fragments arranged by size: 

                 611     332     301     210      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     580    1512 
   Size:        580     932
 
BbvCI CC'TCA_GC

Cuts at:      0     198     934    1512 
   Size:        198     736     578

  Fragments arranged by size: 

                 736     578     198
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      60    1512 
   Size:         60    1452
 
BclI T'GATC_A

Cuts at:      0     978    1512 
   Size:        978     534
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1175    1512 
   Size:       1175     337
 
BglII A'GATC_T

Cuts at:      0    1124    1373    1512 
   Size:       1124     249     139

  Fragments arranged by size: 

                1124     249     139
 
BlpI GC'TnA_GC

Cuts at:      0     610    1512 
   Size:        610     902
 
Bme1580I G_kGCm'C

Cuts at:      0     428     912    1068    1180    1363    1512 
   Size:        428     484     156     112     183     149

  Fragments arranged by size: 

                 484     428     183     156     149     112
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     996    1512 
   Size:        996     516
 
Bpu10I CC'TnA_GC

Cuts at:      0     198     934     964    1512 
   Size:        198     736      30     548

  Fragments arranged by size: 

                 736     548     198      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1480    1512 
   Size:       1480      32
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1033    1512 
   Size:       1033     479
 
BsaAI yAC'GTr

Cuts at:      0     639    1512 
   Size:        639     873
 
BsaBI GATnn'nnATC

Cuts at:      0    1123    1378    1512 
   Size:       1123     255     134

  Fragments arranged by size: 

                1123     255     134
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     601     960    1230    1512 
   Size:        160     441     359     270     282

  Fragments arranged by size: 

                 441     359     282     270     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244    1512 
   Size:        244    1268
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     611     970    1022    1068    1512 
   Size:         41     570     359      52      46     444

  Fragments arranged by size: 

                 570     444     359      52      46      41
 
BsmI GAATG_Cn'

Cuts at:      0     659    1512 
   Size:        659     853
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     361    1512 
   Size:        361    1151
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     428     611     912     970    1022    1068    1180 
   Size:         41     387     183     301      58      52      46     112

Cuts at:   1180    1363    1512 
   Size:        183     149

  Fragments arranged by size: 

                 387     301     183     183     149     112      58      52
                  46      41
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     615    1512 
   Size:        615     897
 
BsrBI CCG'CTC

Cuts at:      0      78    1512 
   Size:         78    1434
 
BsrDI GCAATG_nn'

Cuts at:      0      98     347    1512 
   Size:         98     249    1165

  Fragments arranged by size: 

                1165     249      98
 
BsrFI r'CCGG_y

Cuts at:      0     620    1512 
   Size:        620     892
 
BsrGI T'GTAC_A

Cuts at:      0     894    1512 
   Size:        894     618
 
BstEII G'GTnAC_C

Cuts at:      0     798    1512 
   Size:        798     714
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1440    1512 
   Size:       1440      72
 
BstYI r'GATC_y

Cuts at:      0     668     707    1124    1257    1373    1512 
   Size:        668      39     417     133     116     139

  Fragments arranged by size: 

                 668     417     139     133     116      39
 
Bsu36I CC'TnA_GG

Cuts at:      0     584    1512 
   Size:        584     928
 
BtgI C'CryG_G

Cuts at:      0     551    1106    1512 
   Size:        551     555     406

  Fragments arranged by size: 

                 555     551     406
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1176    1452    1512 
   Size:       1176     276      60

  Fragments arranged by size: 

                1176     276      60
 
BtsI GCAGTG_nn'

Cuts at:      0     371    1512 
   Size:        371    1141
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     440     475    1512 
   Size:        440      35    1037

  Fragments arranged by size: 

                1037     440      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1243    1512 
   Size:       1243     269
 
EaeI y'GGCC_r

Cuts at:      0     215    1154    1167    1512 
   Size:        215     939      13     345

  Fragments arranged by size: 

                 939     345     215      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     559     599    1512 
   Size:        559      40     913

  Fragments arranged by size: 

                 913     559      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     739    1512 
   Size:        739     773
 
EcoICRI GAG'CTC

Cuts at:      0     609     968    1512 
   Size:        609     359     544

  Fragments arranged by size: 

                 609     544     359
 
EcoO109I rG'GnC_Cy

Cuts at:      0     828    1177    1512 
   Size:        828     349     335

  Fragments arranged by size: 

                 828     349     335
 
FspI TGC'GCA

Cuts at:      0    1264    1512 
   Size:       1264     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1027    1059    1225    1257    1300    1332    1512 
   Size:       1027      32     166      32      43      32     180

  Fragments arranged by size: 

                1027     180     166      43      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     227     523    1267    1512 
   Size:        227     296     744     245

  Fragments arranged by size: 

                 744     296     245     227
 
KpnI G_GTAC'C

Cuts at:      0     116    1343    1512 
   Size:        116    1227     169

  Fragments arranged by size: 

                1227     169     116
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     719    1512 
   Size:        719     793
 
MscI TGG'CCA

Cuts at:      0    1156    1169    1512 
   Size:       1156      13     343

  Fragments arranged by size: 

                1156     343      13
 
MslI CAynn'nnrTG

Cuts at:      0      44      71     805     917    1027    1438    1512 
   Size:         44      27     734     112     110     411      74

  Fragments arranged by size: 

                 734     411     112     110      74      44      27
 
MspA1I CmG'CkG

Cuts at:      0     285    1228    1293    1512 
   Size:        285     943      65     219

  Fragments arranged by size: 

                 943     285     219      65
 
NcoI C'CATG_G

Cuts at:      0    1106    1512 
   Size:       1106     406
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     412     831    1512 
   Size:        412     419     681

  Fragments arranged by size: 

                 681     419     412
 
PmlI CAC'GTG

Cuts at:      0     639    1512 
   Size:        639     873
 
PpuMI rG'GwC_Cy

Cuts at:      0     828    1512 
   Size:        828     684
 
PspOMI G'GGCC_C

Cuts at:      0     908    1176    1512 
   Size:        908     268     336

  Fragments arranged by size: 

                 908     336     268
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     887     919    1512 
   Size:        887      32     593

  Fragments arranged by size: 

                 887     593      32
 
PstI C_TGCA'G

Cuts at:      0     290    1512 
   Size:        290    1222
 
PvuII CAG'CTG

Cuts at:      0     285    1293    1512 
   Size:        285    1008     219

  Fragments arranged by size: 

                1008     285     219
 
SacI G_AGCT'C

Cuts at:      0     611     970    1512 
   Size:        611     359     542

  Fragments arranged by size: 

                 611     542     359
 
SapI GCTCTTCn'nnn_

Cuts at:      0     599    1512 
   Size:        599     913
 
ScaI AGT'ACT

Cuts at:      0     985    1512 
   Size:        985     527
 
SexAI A'CCwGG_T

Cuts at:      0     830    1512 
   Size:        830     682
 
SfcI C'TryA_G

Cuts at:      0     286    1223    1503    1512 
   Size:        286     937     280       9

  Fragments arranged by size: 

                 937     286     280       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1175    1512 
   Size:       1175     337
 
SmlI C'TyrA_G

Cuts at:      0    1270    1495    1512 
   Size:       1270     225      17

  Fragments arranged by size: 

                1270     225      17
 
StuI AGG'CCT

Cuts at:      0     932    1512 
   Size:        932     580
 
StyI C'CwwG_G

Cuts at:      0     362    1025    1106    1512 
   Size:        362     663      81     406

  Fragments arranged by size: 

                 663     406     362      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      56     191     451     475     864    1512 
   Size:         56     135     260      24     389     648

  Fragments arranged by size: 

                 648     389     260     135      56      24
 
TatI w'GTAC_w

Cuts at:      0      95     894     983    1512 
   Size:         95     799      89     529

  Fragments arranged by size: 

                 799     529      95      89
 

 Enzymes that do cut:

     AarI   Acc65I    AflII     AleI    AlwNI     ApaI    ApaLI     AvaI
    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI     EarI     EciI
  EcoICRI EcoO109I     FspI    Hin4I  HindIII     KpnI     MmeI     MscI
     MslI   MspA1I     NcoI    PflMI     PmlI    PpuMI   PspOMI     PsrI
     PstI    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SfiI
     SmlI     StuI     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI     BcgI    BfrBI    BmgBI     BmtI     BplI     BpmI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BspEI    BspHI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I     ClaI     DraI   DraIII     EagI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII
   HincII     HpaI     KasI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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