Restriction map for
pF1KB5893
(Circular) (Six-base) MAPSORT of: pF1KB5893.seq Check: 6968 from: 1 to: 4610
pF1KB5893 4610 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AatII G_ACGT'C
Cuts at: 1337 1337
Size: 4610
AccI GT'mk_AC
Cuts at: 1648 1648
Size: 4610
Acc65I G'GTAC_C
Cuts at: 1626 1626
Size: 4610
AclI AA'CG_TT
Cuts at: 4450 4450
Size: 4610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 2435 2867 3614 3882 202
Size: 2233 432 747 268 930
Fragments arranged by size:
2233 930 747 432 268
AfeI AGC'GCT
Cuts at: 583 583
Size: 4610
AflIII A'CryG_T
Cuts at: 1962 3066 4043 1962
Size: 1104 977 2529
Fragments arranged by size:
2529 1104 977
AgeI A'CCGG_T
Cuts at: 2998 2998
Size: 4610
AleI CACnn'nnGTG
Cuts at: 784 1540 784
Size: 756 3854
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1468 1500 1468
Size: 32 4578
AlwNI CAG_nnn'CTG
Cuts at: 744 1915 3482 744
Size: 1171 1567 1872
Fragments arranged by size:
1872 1567 1171
ApaI G_GGCC'C
Cuts at: 1247 1247
Size: 4610
ApaLI G'TGCA_C
Cuts at: 3380 3380
Size: 4610
ApoI r'AATT_y
Cuts at: 1614 3963 4219 1614
Size: 2349 256 2005
Fragments arranged by size:
2349 2005 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
AvaI C'yCGr_G
Cuts at: 152 1630 152
Size: 1478 3132
BamHI G'GATC_C
Cuts at: 1635 1635
Size: 4610
BanI G'GyrC_C
Cuts at: 1626 2040 2288 2323 1626
Size: 414 248 35 3913
Fragments arranged by size:
3913 414 248 35
BanII G_rGCy'C
Cuts at: 149 180 200 689 875 1097 1247 1507 1624
Size: 31 20 489 186 222 150 260 117
Cuts at: 1624 2654 149
Size: 1030 2105
Fragments arranged by size:
2105 1030 489 260 222 186 150 117
31 20
BbeI G_GCGC'C
Cuts at: 2044 2292 2044
Size: 248 4362
BbsI GAAGACnn'nnnn_
Cuts at: 659 1187 1434 659
Size: 528 247 3835
Fragments arranged by size:
3835 528 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 513 547 513
Size: 34 4576
BciVI GTATCCnnnnn_n'
Cuts at: 2508 3269 4610 2508
Size: 761 1341 2508
Fragments arranged by size:
2508 1341 761
BclI T'GATC_A
Cuts at: 1154 2130 1154
Size: 976 3634
BglI GCCn_nnn'nGGC
Cuts at: 367 367
Size: 4610
BglII A'GATC_T
Cuts at: 1040 2125 4162 1040
Size: 1085 2037 1488
Fragments arranged by size:
2037 1488 1085
BlpI GC'TnA_GC
Cuts at: 143 775 1722 143
Size: 632 947 3031
Fragments arranged by size:
3031 947 632
Bme1580I G_kGCm'C
Cuts at: 1247 2235 2328 3384 1247
Size: 988 93 1056 2473
Fragments arranged by size:
2473 1056 988 93
BmrI ACTGGGnnnn_n'
Cuts at: 442 1125 1822 1903 2238 4382 442
Size: 683 697 81 335 2144 670
Fragments arranged by size:
2144 697 683 670 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 783 2977 783
Size: 2194 2416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 1483
Size: 4610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1786 2665 3157 3455 184
Size: 1602 879 492 298 1339
Fragments arranged by size:
1602 1339 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 886 4314 886
Size: 3428 1182
BsaAI yAC'GTr
Cuts at: 1963 1963
Size: 4610
BsaBI GATnn'nnATC
Cuts at: 6 2124 4161 6
Size: 2118 2037 455
Fragments arranged by size:
2118 2037 455
BsaHI Gr'CG_yC
Cuts at: 359 1334 2041 2289 2991 4487 359
Size: 975 707 248 702 1496 482
Fragments arranged by size:
1496 975 707 702 482 248
BsaWI w'CCGG_w
Cuts at: 722 1205 1799 2320 2998 3272 3419 3830 722
Size: 483 594 521 678 274 147 411 1502
Fragments arranged by size:
1502 678 594 521 483 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 568 598 919 949 1468 1498 568
Size: 30 321 30 519 30 3680
Fragments arranged by size:
3680 519 321 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 847 208
Size: 639 3971
BseYI C'CCAG_C
Cuts at: 283 351 2035 2793 3370 3866 283
Size: 68 1684 758 577 496 1027
Fragments arranged by size:
1684 1027 758 577 496 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 703
Size: 4610
BsiEI CG_ry'CG
Cuts at: 104 2152 2198 3406 104
Size: 2048 46 1208 1308
Fragments arranged by size:
2048 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 180 200 578 689 781 875 1097 1624 2402
Size: 20 378 111 92 94 222 527 778
Cuts at: 2402 2592 3384 180
Size: 190 792 1406
Fragments arranged by size:
1406 792 778 527 378 222 190 111
94 92 20
BsmBI CGTCTCn'nnnn_
Cuts at: 350 460 350
Size: 110 4500
Bsp1286I G_dGCh'C
Cuts at: 149 180 200 578 689 781 875 1097 1247
Size: 31 20 378 111 92 94 222 150
Cuts at: 1247 1507 1624 2235 2328 2402 2592 2654 3384
Size: 260 117 611 93 74 190 62 730
Cuts at: 3384 149
Size: 1375
Fragments arranged by size:
1375 730 611 378 260 222 190 150
117 111 94 93 92 74 62 31
20
BspEI T'CCGG_A
Cuts at: 1205 3830 1205
Size: 2625 1985
BspHI T'CATG_A
Cuts at: 677 3786 677
Size: 3109 1501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 1265 1660 1874 2176 2557 332
Size: 933 395 214 302 381 2385
Fragments arranged by size:
2385 933 395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 555 1218 2902 2956 4440 4605 50
Size: 505 663 1684 54 1484 165 55
Fragments arranged by size:
1684 1484 663 505 165 55 54
BsrDI GCAATG_nn'
Cuts at: 294 2522 294
Size: 2228 2382
BsrFI r'CCGG_y
Cuts at: 2608 2789 2998 2608
Size: 181 209 4220
Fragments arranged by size:
4220 209 181
BssSI C'ACGA_G
Cuts at: 784 1018 2881 3239 784
Size: 234 1863 358 2155
Fragments arranged by size:
2155 1863 358 234
BstAPI GCAn_nnn'nTGC
Cuts at: 335 1987 335
Size: 1652 2958
BstBI TT'CG_AA
Cuts at: 2971 2971
Size: 4610
BstXI CCAn_nnnn'nTGG
Cuts at: 114 378 426 114
Size: 264 48 4298
Fragments arranged by size:
4298 264 48
BstYI r'GATC_y
Cuts at: 1 1040 1269 1635 2102 2125 2460 2706 3718
Size: 1039 229 366 467 23 335 246 1012
Cuts at: 3718 3804 3816 4162 1
Size: 86 12 346 449
Fragments arranged by size:
1039 1012 467 449 366 346 335 246
229 86 23 12
BtgI C'CryG_G
Cuts at: 107 587 837 1565 107
Size: 480 250 728 3152
Fragments arranged by size:
3152 728 480 250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 127 546 2560 2741 2904 127
Size: 419 2014 181 163 1833
Fragments arranged by size:
2014 1833 419 181 163
BtsI GCAGTG_nn'
Cuts at: 700 1547 1853 700
Size: 847 306 3457
Fragments arranged by size:
3457 847 306
DraI TTT'AAA
Cuts at: 1609 1609
Size: 4610
DraIII CAC_nnn'GTG
Cuts at: 335 335
Size: 4610
DrdI GACnn_nn'nnGTC
Cuts at: 2316 3174 2316
Size: 858 3752
EaeI y'GGCC_r
Cuts at: 840 2195 2369 2760 2787 4132 840
Size: 1355 174 391 27 1345 1318
Fragments arranged by size:
1355 1345 1318 391 174 27
EagI C'GGCC_G
Cuts at: 2195 2195
Size: 4610
EarI CTCTTCn'nnn_
Cuts at: 1349 2633 2843 1349
Size: 1284 210 3116
Fragments arranged by size:
3116 1284 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 2818 3128 3274 4420 133
Size: 2685 310 146 1146 323
Fragments arranged by size:
2685 1146 323 310 146
EcoICRI GAG'CTC
Cuts at: 178 198 687 873 1095 1622 178
Size: 20 489 186 222 527 3166
Fragments arranged by size:
3166 527 489 222 186 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1483 2435 2867 3614 3882 202
Size: 1281 952 432 747 268 930
Fragments arranged by size:
1281 952 930 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 762 1243 1749 762
Size: 481 506 3623
Fragments arranged by size:
3623 506 481
EcoRI G'AATT_C
Cuts at: 1614 1614
Size: 4610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1365 2629 1365
Size: 1264 3346
FspI TGC'GCA
Cuts at: 328 2391 328
Size: 2063 2547
HaeII r_GCGC'y
Cuts at: 585 1293 2044 2292 3314 4293 585
Size: 708 751 248 1022 979 902
Fragments arranged by size:
1022 979 902 751 708 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 919 951 2452 2482 2484 2514 919
Size: 32 1501 30 2 30 3015
Fragments arranged by size:
3015 1501 32 30 30 2
HincII GTy'rAC
Cuts at: 1649 1649
Size: 4610
HindIII A'AGCT_T
Cuts at: 646 646
Size: 4610
KasI G'GCGC_C
Cuts at: 2040 2288 2040
Size: 248 4362
KpnI G_GTAC'C
Cuts at: 1630 1630
Size: 4610
MluI A'CGCG_T
Cuts at: 4043 4043
Size: 4610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3281 3465 3281
Size: 184 4426
MscI TGG'CCA
Cuts at: 842 2371 842
Size: 1529 3081
MslI CAynn'nnrTG
Cuts at: 112 784 1540 2599 2726 112
Size: 672 756 1059 127 1996
Fragments arranged by size:
1996 1059 756 672 127
MspA1I CmG'CkG
Cuts at: 638 1430 1721 2035 2395 3408 3653 638
Size: 792 291 314 360 1013 245 1595
Fragments arranged by size:
1595 1013 792 360 314 291 245
NaeI GCC'GGC
Cuts at: 2791 2791
Size: 4610
NarI GG'CG_CC
Cuts at: 2041 2289 2041
Size: 248 4362
NcoI C'CATG_G
Cuts at: 107 837 1565 107
Size: 730 728 3152
Fragments arranged by size:
3152 730 728
NgoMIV G'CCGG_C
Cuts at: 2789 2789
Size: 4610
NspI r_CATG'y
Cuts at: 1663 2163 3070 1663
Size: 500 907 3203
Fragments arranged by size:
3203 907 500
PciI A'CATG_T
Cuts at: 3066 3066
Size: 4610
PflMI CCAn_nnn'nTGG
Cuts at: 744 744
Size: 4610
PmeI GTTT'AAAC
Cuts at: 1609 1609
Size: 4610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1276 1308 1468 1500 3749 3781 1276
Size: 32 160 32 2249 32 2105
Fragments arranged by size:
2249 2105 160 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 762 762
Size: 4610
PshAI GACnn'nnGTC
Cuts at: 472 472
Size: 4610
PspOMI G'GGCC_C
Cuts at: 1243 1243
Size: 4610
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1027 1059 1027
Size: 32 4578
PstI C_TGCA'G
Cuts at: 1592 1657 2342 1592
Size: 65 685 3860
Fragments arranged by size:
3860 685 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4610
PvuII CAG'CTG
Cuts at: 2035 2395 2035
Size: 360 4250
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 4610
SacI G_AGCT'C
Cuts at: 180 200 689 875 1097 1624 180
Size: 20 489 186 222 527 3166
Fragments arranged by size:
3166 527 489 222 186 20
SalI G'TCGA_C
Cuts at: 1647 1647
Size: 4610
SapI GCTCTTCn'nnn_
Cuts at: 2633 2843 2633
Size: 210 4400
SbfI CC_TGCA'GG
Cuts at: 1657 1657
Size: 4610
ScaI AGT'ACT
Cuts at: 4127 4127
Size: 4610
SfcI C'TryA_G
Cuts at: 33 258 944 1588 1653 2338 3331 3522 33
Size: 225 686 644 65 685 993 191 1121
Fragments arranged by size:
1121 993 686 685 644 225 191 65
SfoI GGC'GCC
Cuts at: 2042 2290 2042
Size: 248 4362
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
SmaI CCC'GGG
Cuts at: 154 1632 154
Size: 1478 3132
SmlI C'TyrA_G
Cuts at: 199 1765 2680 3172 3434 199
Size: 1566 915 492 262 1375
Fragments arranged by size:
1566 1375 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4610
SphI G_CATG'C
Cuts at: 1663 2163 1663
Size: 500 4110
StuI AGG'CCT
Cuts at: 547 1169 1345 547
Size: 622 176 3812
Fragments arranged by size:
3812 622 176
StyI C'CwwG_G
Cuts at: 107 230 837 1340 1565 1744 2098 107
Size: 123 607 503 225 179 354 2619
Fragments arranged by size:
2619 607 503 354 225 179 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 850 944 2193 2326 2785 2994 850
Size: 94 1249 133 459 209 2466
Fragments arranged by size:
2466 1249 459 209 133 94
TatI w'GTAC_w
Cuts at: 389 632 1073 4125 389
Size: 243 441 3052 874
Fragments arranged by size:
3052 874 441 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 123 155 568 600 4127 4159 123
Size: 32 413 32 3527 32 574
Fragments arranged by size:
3527 574 413 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1186 2407 1186
Size: 1221 3389
XbaI T'CTAG_A
Cuts at: 1641 1641
Size: 4610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1347 2105 1347
Size: 758 3852
XmaI C'CCGG_G
Cuts at: 152 1630 152
Size: 1478 3132
ZraI GAC'GTC
Cuts at: 1335 1335
Size: 4610
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI