Restriction map for pF1KB5893
 (Linear) (Six-base) MAPSORT of: KIBB5893.seq  Check: 1041  from: 1  to: 1497



KIBB5893.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AatII G_ACGT'C

Cuts at:      0    1229    1497 
   Size:       1229     268
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94    1497 
   Size:         94    1403
 
AfeI AGC'GCT

Cuts at:      0     475    1497 
   Size:        475    1022
 
AleI CACnn'nnGTG

Cuts at:      0     676    1432    1497 
   Size:        676     756      65

  Fragments arranged by size: 

                 756     676      65
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1360    1392    1497 
   Size:       1360      32     105

  Fragments arranged by size: 

                1360     105      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     636    1497 
   Size:        636     861
 
ApaI G_GGCC'C

Cuts at:      0    1139    1497 
   Size:       1139     358
 
AvaI C'yCGr_G

Cuts at:      0      44    1497 
   Size:         44    1453
 
BanII G_rGCy'C

Cuts at:      0      41      72      92     581     767     989    1139    1399 
   Size:         41      31      20     489     186     222     150     260

Cuts at:   1399    1497 
   Size:         98

  Fragments arranged by size: 

                 489     260     222     186     150      98      41      31
                  20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     551    1079    1326    1497 
   Size:        551     528     247     171

  Fragments arranged by size: 

                 551     528     247     171
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     405     439    1497 
   Size:        405      34    1058

  Fragments arranged by size: 

                1058     405      34
 
BclI T'GATC_A

Cuts at:      0    1046    1497 
   Size:       1046     451
 
BglI GCCn_nnn'nGGC

Cuts at:      0     259    1497 
   Size:        259    1238
 
BglII A'GATC_T

Cuts at:      0     932    1497 
   Size:        932     565
 
BlpI GC'TnA_GC

Cuts at:      0      35     667    1497 
   Size:         35     632     830

  Fragments arranged by size: 

                 830     632      35
 
Bme1580I G_kGCm'C

Cuts at:      0    1139    1497 
   Size:       1139     358
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     334    1017    1497 
   Size:        334     683     480

  Fragments arranged by size: 

                 683     480     334
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     675    1497 
   Size:        675     822
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1375    1497 
   Size:       1375     122
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1497 
   Size:         76    1421
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     778    1497 
   Size:        778     719
 
BsaHI Gr'CG_yC

Cuts at:      0     251    1226    1497 
   Size:        251     975     271

  Fragments arranged by size: 

                 975     271     251
 
BsaWI w'CCGG_w

Cuts at:      0     614    1097    1497 
   Size:        614     483     400

  Fragments arranged by size: 

                 614     483     400
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     460     490     811     841    1360    1390    1497 
   Size:        460      30     321      30     519      30     107

  Fragments arranged by size: 

                 519     460     321     107      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     739    1497 
   Size:        100     639     758

  Fragments arranged by size: 

                 758     639     100
 
BseYI C'CCAG_C

Cuts at:      0     175     243    1497 
   Size:        175      68    1254

  Fragments arranged by size: 

                1254     175      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    1497 
   Size:        595     902
 
BsiHKAI G_wGCw'C

Cuts at:      0      72      92     470     581     673     767     989    1497 
   Size:         72      20     378     111      92      94     222     508

  Fragments arranged by size: 

                 508     378     222     111      94      92      72      20
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     242     352    1497 
   Size:        242     110    1145

  Fragments arranged by size: 

                1145     242     110
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      72      92     470     581     673     767     989 
   Size:         41      31      20     378     111      92      94     222

Cuts at:    989    1139    1399    1497 
   Size:        150     260      98

  Fragments arranged by size: 

                 378     260     222     150     111      98      94      92
                  41      31      20
 
BspEI T'CCGG_A

Cuts at:      0    1097    1497 
   Size:       1097     400
 
BspHI T'CATG_A

Cuts at:      0     569    1497 
   Size:        569     928
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     224    1157    1497 
   Size:        224     933     340

  Fragments arranged by size: 

                 933     340     224
 
BsrBI CCG'CTC

Cuts at:      0     447    1110    1497 
   Size:        447     663     387

  Fragments arranged by size: 

                 663     447     387
 
BsrDI GCAATG_nn'

Cuts at:      0     186    1497 
   Size:        186    1311
 
BssSI C'ACGA_G

Cuts at:      0     676     910    1497 
   Size:        676     234     587

  Fragments arranged by size: 

                 676     587     234
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     227    1497 
   Size:        227    1270
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     270     318    1497 
   Size:        270      48    1179

  Fragments arranged by size: 

                1179     270      48
 
BstYI r'GATC_y

Cuts at:      0     932    1161    1497 
   Size:        932     229     336

  Fragments arranged by size: 

                 932     336     229
 
BtgI C'CryG_G

Cuts at:      0     479     729    1457    1497 
   Size:        479     250     728      40

  Fragments arranged by size: 

                 728     479     250      40
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      19     438    1497 
   Size:         19     419    1059

  Fragments arranged by size: 

                1059     419      19
 
BtsI GCAGTG_nn'

Cuts at:      0     592    1439    1497 
   Size:        592     847      58

  Fragments arranged by size: 

                 847     592      58
 
DraIII CAC_nnn'GTG

Cuts at:      0     227    1497 
   Size:        227    1270
 
EaeI y'GGCC_r

Cuts at:      0     732    1497 
   Size:        732     765
 
EarI CTCTTCn'nnn_

Cuts at:      0    1241    1497 
   Size:       1241     256
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      25    1497 
   Size:         25    1472
 
EcoICRI GAG'CTC

Cuts at:      0      70      90     579     765     987    1497 
   Size:         70      20     489     186     222     510

  Fragments arranged by size: 

                 510     489     222     186      70      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94    1375    1497 
   Size:         94    1281     122

  Fragments arranged by size: 

                1281     122      94
 
EcoO109I rG'GnC_Cy

Cuts at:      0     654    1135    1497 
   Size:        654     481     362

  Fragments arranged by size: 

                 654     481     362
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1257    1497 
   Size:       1257     240
 
FspI TGC'GCA

Cuts at:      0     220    1497 
   Size:        220    1277
 
HaeII r_GCGC'y

Cuts at:      0     477    1185    1497 
   Size:        477     708     312

  Fragments arranged by size: 

                 708     477     312
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     811     843    1497 
   Size:        811      32     654

  Fragments arranged by size: 

                 811     654      32
 
HindIII A'AGCT_T

Cuts at:      0     538    1497 
   Size:        538     959
 
MscI TGG'CCA

Cuts at:      0     734    1497 
   Size:        734     763
 
MslI CAynn'nnrTG

Cuts at:      0     676    1432    1497 
   Size:        676     756      65

  Fragments arranged by size: 

                 756     676      65
 
MspA1I CmG'CkG

Cuts at:      0     530    1322    1497 
   Size:        530     792     175

  Fragments arranged by size: 

                 792     530     175
 
NcoI C'CATG_G

Cuts at:      0     729    1457    1497 
   Size:        729     728      40

  Fragments arranged by size: 

                 729     728      40
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     636    1497 
   Size:        636     861
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1168    1200    1360    1392    1497 
   Size:       1168      32     160      32     105

  Fragments arranged by size: 

                1168     160     105      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     654    1497 
   Size:        654     843
 
PshAI GACnn'nnGTC

Cuts at:      0     364    1497 
   Size:        364    1133
 
PspOMI G'GGCC_C

Cuts at:      0    1135    1497 
   Size:       1135     362
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     919     951    1497 
   Size:        919      32     546

  Fragments arranged by size: 

                 919     546      32
 
PstI C_TGCA'G

Cuts at:      0    1484    1497 
   Size:       1484      13
 
SacI G_AGCT'C

Cuts at:      0      72      92     581     767     989    1497 
   Size:         72      20     489     186     222     508

  Fragments arranged by size: 

                 508     489     222     186      72      20
 
SfcI C'TryA_G

Cuts at:      0     150     836    1480    1497 
   Size:        150     686     644      17

  Fragments arranged by size: 

                 686     644     150      17
 
SmaI CCC'GGG

Cuts at:      0      46    1497 
   Size:         46    1451
 
SmlI C'TyrA_G

Cuts at:      0      91    1497 
   Size:         91    1406
 
StuI AGG'CCT

Cuts at:      0     439    1061    1237    1497 
   Size:        439     622     176     260

  Fragments arranged by size: 

                 622     439     260     176
 
StyI C'CwwG_G

Cuts at:      0     122     729    1232    1457    1497 
   Size:        122     607     503     225      40

  Fragments arranged by size: 

                 607     503     225     122      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     742     836    1497 
   Size:        742      94     661

  Fragments arranged by size: 

                 742     661      94
 
TatI w'GTAC_w

Cuts at:      0     281     524     965    1497 
   Size:        281     243     441     532

  Fragments arranged by size: 

                 532     441     281     243
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      15      47     460     492    1497 
   Size:         15      32     413      32    1005

  Fragments arranged by size: 

                1005     413      32      32      15
 
Tth111I GACn'n_nGTC

Cuts at:      0    1078    1497 
   Size:       1078     419
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1239    1497 
   Size:       1239     258
 
XmaI C'CCGG_G

Cuts at:      0      44    1497 
   Size:         44    1453
 
ZraI GAC'GTC

Cuts at:      0    1227    1497 
   Size:       1227     270
 

 Enzymes that do cut:

    AatII     AcuI     AfeI     AleI     AloI    AlwNI     ApaI     AvaI
    BanII     BbsI     BcgI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BssSI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FalI     FspI    HaeII    Hin4I  HindIII     MscI     MslI
   MspA1I     NcoI    PflMI     PpiI    PpuMI    PshAI   PspOMI     PsrI
     PstI     SacI     SfcI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI    BciVI    BfrBI    BmgBI     BmtI
   Bpu10I    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BsrFI    BsrGI
   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PsiI    PspXI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     XmnI
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