Restriction map for pF1KB5865
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# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AvrII       BanI        BbeI        BbrPI       
BciVI       BfrI        BglI        BglII       BlnI        
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoT22I     
FauI        FseI        HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SspBI       SwaI        
Tru9I       XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII         991  CGCG                 CG^CG
AcsI          409  AAATTC               [GA]^AATT[CT]
AcyI         1204  GGCGTC               G[GA]^CG[CT]C
Alw44I        398  GTGCAC               G^TGCAC
AocI          945  CCTGAGG              CC^T[ACGT]AGG
ApaLI         398  GTGCAC               G^TGCAC
ApoI          409  AAATTC               [GA]^AATT[CT]
Asp700I      1026  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1012  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         654  TGCGCA               TGC^GCA
BamHI         262  GGATCC               G^GATCC
BbvCI         463  GCTGAGG              CCTCAGC(-5/-2)
BcnI         1224  CCGGG                CC^[GC]GG
BfaI           26  CTAG                 C^TAG
BlpI         1048  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1048  GCTGAGC              GC^T[ACGT]AGC
BsaAI         268  CACGTA               [CT]AC^GT[GA]
BsaHI        1204  GGCGTC               G[GA]^CG[CT]C
BsgI          958  GTGCAG               GTGCAG(16/14)
BsiWI         270  CGTACG               C^GTACG
BsmAI         535  GAGAC                GTCTC(1/5)
BsrBI         844  CCGCTC               CCGCTC(-3/-3)
BssSI         721  CTCGTG               CACGAG(-5/-1)
BstUI         991  CGCG                 CG^CG
BstXI        1111  CCAGGCCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        945  CCTGAGG              CC^T[ACGT]AGG
CelII        1048  GCTGAGC              GC^T[ACGT]AGC
DsaI          833  CCACGG               C^C[GA][CT]GG
Eco81I        945  CCTGAGG              CC^T[ACGT]AGG
EcoRV         358  GATATC               GAT^ATC
EcoT14I       773  CCAAGG               C^C[AT][AT]GG
FspI          654  TGCGCA               TGC^GCA
HapII        1224  CCGG                 C^CGG
Hin1I        1204  GGCGTC               G[GA]^CG[CT]C
HincII       1357  GTCAAC               GT[CT]^[GA]AC
HindII       1357  GTCAAC               GT[CT]^[GA]AC
HinfI         279  GAATC                G^A[ACGT]TC
HpaII        1224  CCGG                 C^CGG
MaeI           26  CTAG                 C^TAG
MaeII         269  ACGT                 A^CGT
MslI          972  CACGGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        910  CAGCGG               C[AC]G^C[GT]G
MspI         1224  CCGG                 C^CGG
MvnI          991  CGCG                 CG^CG
NciI         1224  CCGGG                CC^[GC]GG
PflFI        1012  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SmlI          391  CTCAAG               C^T[CT][GA]AG
SphI           44  GCATGC               GCATG^C
SspI          629  AATATT               AAT^ATT
StuI         1113  AGGCCT               AGG^CCT
StyI          773  CCAAGG               C^C[AT][AT]GG
TaqI          794  TCGA                 T^CGA
TfiI          279  GAATC                G^A[AT]TC
Tth111I      1012  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       794  TCGA                 T^CGA
XmnI         1026  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AvaI          928  CTCGGG               C^[CT]CG[GA]G
             1165  CCCGAG              
BbsI          445  GAAGAC               GAAGAC(2/6)
              680  GTCTTC              
BcgI         1167  CGAGCATGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1083  GCAGCAGTATCG        
BclI          563  TGATCA               T^GATCA
              692  TGATCA              
BpuAI         445  GAAGAC               GAAGAC(2/6)
              680  GTCTTC              
BsoBI         928  CTCGGG               C^[CT]CG[GA]G
             1165  CCCGAG              
BstAPI        657  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              810  GCAGAAGCTGC         
BstYI         262  GGATCC               [GA]^GATC[CT]
              387  GGATCT              
EcoNI         720  CCTCGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              954  CCTGGTGCAGG         
FbaI          563  TGATCA               T^GATCA
              692  TGATCA              
HaeII         751  GGCGCT               [GA]GCGC^[CT]
             1158  GGCGCT              
HgaI          216  GACGC                GACGC(5/10)
             1205  GCGTC               
MaeIII        997  GTCAC                ^GT[ACGT]AC
             1018  GTCAC               
MflI          262  GGATCC               [GA]^GATC[CT]
              387  GGATCT              
NspI           44  GCATGC               [GA]CATG^[CT]
              542  ACATGC              
SapI          132  GAAGAGC              GCTCTTC(1/4)
              477  GCTCTTC             
SbfI          256  CCTGCAGG             CCTGCA^GG
             1272  CCTGCAGG            
SexAI        1013  ACCTGGT              A^CC[AT]GGT
             1286  ACCAGGT             
SfaNI         187  GATGC                GCATC(5/9)
             1174  GATGC               
Sse8387I      256  CCTGCAGG             CCTGCA^GG
             1272  CCTGCAGG            
Tsp45I        997  GTCAC                ^GT[GC]AC
             1018  GTCAC               
Tsp509I       337  AATT                 ^AATT
              410  AATT                
XcmI          584  CCAGCTCCAGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              773  CCAAGGTCCAGGTGG     
XhoII         262  GGATCC               [GA]^GATC[CT]
              387  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BalI        BanII       BbvI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HphI        
ItaI        Ksp632I     MboI        MboII       MluNI       
MnlI        MscI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PflMI       PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
TseI        TspRI       Van91I      
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