Restriction map for pF1KB5815
 (Circular) (Six-base) MAPSORT of: pF1KB5815.seq  Check: 945  from: 1  to: 4526



pF1KB5815  4526 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AccI GT'mk_AC

Cuts at:   1564    1564 
   Size:       4526
 
Acc65I G'GTAC_C

Cuts at:   1542    1542 
   Size:       4526
 
AclI AA'CG_TT

Cuts at:   4366    4366 
   Size:       4526
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2351    2783    3530    3798    2351 
   Size:        432     747     268    3079

  Fragments arranged by size: 

                3079     747     432     268
 
AflIII A'CryG_T

Cuts at:   1878    2982    3959    1878 
   Size:       1104     977    2445

  Fragments arranged by size: 

                2445    1104     977
 
AgeI A'CCGG_T

Cuts at:   2914    2914 
   Size:       4526
 
AleI CACnn'nnGTG

Cuts at:   1408    1408 
   Size:       4526
 
AlwNI CAG_nnn'CTG

Cuts at:    463    1440    1831    3398     463 
   Size:        977     391    1567    1591

  Fragments arranged by size: 

                1591    1567     977     391
 
ApaI G_GGCC'C

Cuts at:    959     959 
   Size:       4526
 
ApaLI G'TGCA_C

Cuts at:   3296    3296 
   Size:       4526
 
ApoI r'AATT_y

Cuts at:    693    1530    3879    4135     693 
   Size:        837    2349     256    1084

  Fragments arranged by size: 

                2349    1084     837     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4526
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4526
 
AvaI C'yCGr_G

Cuts at:    272    1546     272 
   Size:       1274    3252
 
BamHI G'GATC_C

Cuts at:   1551    1551 
   Size:       4526
 
BanI G'GyrC_C

Cuts at:    516     554    1371    1542    1956    2204    2239     516 
   Size:         38     817     171     414     248      35    2803

  Fragments arranged by size: 

                2803     817     414     248     171      38      35
 
BanII G_rGCy'C

Cuts at:    959    1001    1192    1540    2570     959 
   Size:         42     191     348    1030    2915

  Fragments arranged by size: 

                2915    1030     348     191      42
 
BbeI G_GCGC'C

Cuts at:    558    1960    2208     558 
   Size:       1402     248    2876

  Fragments arranged by size: 

                2876    1402     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   2424    3185    4526    2424 
   Size:        761    1341    2424

  Fragments arranged by size: 

                2424    1341     761
 
BclI T'GATC_A

Cuts at:   2046    2046 
   Size:       4526
 
BglI GCCn_nnn'nGGC

Cuts at:   1445    1445 
   Size:       4526
 
BglII A'GATC_T

Cuts at:   1388    2041    4078    1388 
   Size:        653    2037    1836

  Fragments arranged by size: 

                2037    1836     653
 
BlpI GC'TnA_GC

Cuts at:   1138    1638    1138 
   Size:        500    4026
 
Bme1580I G_kGCm'C

Cuts at:    521     959    1376    2151    2244    3300     521 
   Size:        438     417     775      93    1056    1747

  Fragments arranged by size: 

                1747    1056     775     438     417      93
 
BmgBI CAC'GTC

Cuts at:    387     387 
   Size:       4526
 
BmrI ACTGGGnnnn_n'

Cuts at:    390     655    1738    1819    2154    4298     390 
   Size:        265    1083      81     335    2144     618

  Fragments arranged by size: 

                2144    1083     618     335     265      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    732     849    1422    2893     732 
   Size:        117     573    1471    2365

  Fragments arranged by size: 

                2365    1471     573     117
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    946    1702    2581    3073    3371     946 
   Size:        756     879     492     298    2101

  Fragments arranged by size: 

                2101     879     756     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4230    4230 
   Size:       4526
 
BsaAI yAC'GTr

Cuts at:    564    1029    1879     564 
   Size:        465     850    3211

  Fragments arranged by size: 

                3211     850     465
 
BsaBI GATnn'nnATC

Cuts at:      6    2040    4077       6 
   Size:       2034    2037     455

  Fragments arranged by size: 

                2037    2034     455
 
BsaHI Gr'CG_yC

Cuts at:    555    1156    1364    1957    2205    2907    4403     555 
   Size:        601     208     593     248     702    1496     678

  Fragments arranged by size: 

                1496     702     678     601     593     248     208
 
BsaWI w'CCGG_w

Cuts at:   1715    2236    2914    3188    3335    3746    1715 
   Size:        521     678     274     147     411    2495

  Fragments arranged by size: 

                2495     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    334     607    1270    1288    1297     334 
   Size:        273     663      18       9    3563

  Fragments arranged by size: 

                3563     663     273      18       9
 
BseYI C'CCAG_C

Cuts at:   1080    1951    2709    3286    3782    1080 
   Size:        871     758     577     496    1824

  Fragments arranged by size: 

                1824     871     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    694    1219    1332     694 
   Size:        525     113    3888

  Fragments arranged by size: 

                3888     525     113
 
BsiEI CG_ry'CG

Cuts at:    104     243    2068    2114    3322     104 
   Size:        139    1825      46    1208    1308

  Fragments arranged by size: 

                1825    1308    1208     139      46
 
BsiHKAI G_wGCw'C

Cuts at:    326     410    1144    1192    1540    2318    2508    3300     326 
   Size:         84     734      48     348     778     190     792    1552

  Fragments arranged by size: 

                1552     792     778     734     348     190      84      48
            
 
BsmI GAATG_Cn'

Cuts at:   1134    1134 
   Size:       4526
 
Bsp1286I G_dGCh'C

Cuts at:    326     410     521     959    1001    1144    1192    1376    1540 
   Size:         84     111     438      42     143      48     184     164

Cuts at:   1540    2151    2244    2318    2508    2570    3300     326 
   Size:        611      93      74     190      62     730    1552

  Fragments arranged by size: 

                1552     730     611     438     190     184     164     143
                 111      93      84      74      62      48      42
 
BspEI T'CCGG_A

Cuts at:   3746    3746 
   Size:       4526
 
BspHI T'CATG_A

Cuts at:   1235    3702    1235 
   Size:       2467    2059
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1576    1790    2092    2473    1576 
   Size:        214     302     381    3629

  Fragments arranged by size: 

                3629     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     132     159     200     716    2818    2872    4356    4521 
   Size:         82      27      41     516    2102      54    1484     165

Cuts at:   4521      50 
   Size:         55

  Fragments arranged by size: 

                2102    1484     516     165      82      55      54      41
                  27
 
BsrDI GCAATG_nn'

Cuts at:   1055    2438    1055 
   Size:       1383    3143
 
BsrFI r'CCGG_y

Cuts at:    173     242     513    2524    2705    2914     173 
   Size:         69     271    2011     181     209    1785

  Fragments arranged by size: 

                2011    1785     271     209     181      69
 
BssSI C'ACGA_G

Cuts at:    352    2797    3155     352 
   Size:       2445     358    1723

  Fragments arranged by size: 

                2445    1723     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1903    1903 
   Size:       4526
 
BstBI TT'CG_AA

Cuts at:   2887    2887 
   Size:       4526
 
BstXI CCAn_nnnn'nTGG

Cuts at:    652    1467     652 
   Size:        815    3711
 
BstYI r'GATC_y

Cuts at:      1     782    1343    1388    1551    2018    2041    2376    2622 
   Size:        781     561      45     163     467      23     335     246

Cuts at:   2622    3634    3720    3732    4078       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     781     561     467     449     346     335     246
                 163      86      45      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1253    1253 
   Size:       4526
 
BtgI C'CryG_G

Cuts at:    521     992     521 
   Size:        471    4055
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2476    2657    2820    2476 
   Size:        181     163    4182

  Fragments arranged by size: 

                4182     181     163
 
BtsI GCAGTG_nn'

Cuts at:    461    1769     461 
   Size:       1308    3218
 
DraI TTT'AAA

Cuts at:   1525    1525 
   Size:       4526
 
DrdI GACnn_nn'nnGTC

Cuts at:   1039    2232    3090    1039 
   Size:       1193     858    2475

  Fragments arranged by size: 

                2475    1193     858
 
EaeI y'GGCC_r

Cuts at:   1209    2111    2285    2676    2703    4048    1209 
   Size:        902     174     391      27    1345    1687

  Fragments arranged by size: 

                1687    1345     902     391     174      27
 
EagI C'GGCC_G

Cuts at:   2111    2111 
   Size:       4526
 
EarI CTCTTCn'nnn_

Cuts at:    473    2549    2759     473 
   Size:       2076     210    2240

  Fragments arranged by size: 

                2240    2076     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2734    3044    3190    4336    2734 
   Size:        310     146    1146    2924

  Fragments arranged by size: 

                2924    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1190    1538    1190 
   Size:        348    4178
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   2351    2783    3530    3798    2351 
   Size:        432     747     268    3079

  Fragments arranged by size: 

                3079     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    979     979 
   Size:       4526
 
EcoO109I rG'GnC_Cy

Cuts at:    648     956    1224    1437    1665     648 
   Size:        308     268     213     228    3509

  Fragments arranged by size: 

                3509     308     268     228     213
 
EcoRI G'AATT_C

Cuts at:   1530    1530 
   Size:       4526
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2545    2545 
   Size:       4526
 
FspI TGC'GCA

Cuts at:   2307    2307 
   Size:       4526
 
HaeII r_GCGC'y

Cuts at:    269     314     558    1960    2208    3230    4209     269 
   Size:         45     244    1402     248    1022     979     586

  Fragments arranged by size: 

                1402    1022     979     586     248     244      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1457    1480    1489    1512    2368    2398    2400    2430    1457 
   Size:         23       9      23     856      30       2      30    3553

  Fragments arranged by size: 

                3553     856      30      30      23      23       9       2
            
 
HincII GTy'rAC

Cuts at:   1565    1565 
   Size:       4526
 
KasI G'GCGC_C

Cuts at:    554    1956    2204     554 
   Size:       1402     248    2876

  Fragments arranged by size: 

                2876    1402     248
 
KpnI G_GTAC'C

Cuts at:   1546    1546 
   Size:       4526
 
MluI A'CGCG_T

Cuts at:   3959    3959 
   Size:       4526
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:     92    3197    3381      92 
   Size:       3105     184    1237

  Fragments arranged by size: 

                3105    1237     184
 
MscI TGG'CCA

Cuts at:   2287    2287 
   Size:       4526
 
MslI CAynn'nnrTG

Cuts at:    418    1240    1408    2515    2642     418 
   Size:        822     168    1107     127    2302

  Fragments arranged by size: 

                2302    1107     822     168     127
 
MspA1I CmG'CkG

Cuts at:    261    1080    1637    1951    2311    3324    3569     261 
   Size:        819     557     314     360    1013     245    1218

  Fragments arranged by size: 

                1218    1013     819     557     360     314     245
 
NaeI GCC'GGC

Cuts at:   2707    2707 
   Size:       4526
 
NarI GG'CG_CC

Cuts at:    555    1957    2205     555 
   Size:       1402     248    2876

  Fragments arranged by size: 

                2876    1402     248
 
NgoMIV G'CCGG_C

Cuts at:   2705    2705 
   Size:       4526
 
NspI r_CATG'y

Cuts at:    822    1077    1579    2079    2986     822 
   Size:        255     502     500     907    2362

  Fragments arranged by size: 

                2362     907     502     500     255
 
PciI A'CATG_T

Cuts at:   2982    2982 
   Size:       4526
 
PflMI CCAn_nnn'nTGG

Cuts at:    741     741 
   Size:       4526
 
PmeI GTTT'AAAC

Cuts at:   1525    1525 
   Size:       4526
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3665    3697    3665 
   Size:         32    4494
 
PpuMI rG'GwC_Cy

Cuts at:   1224    1224 
   Size:       4526
 
PshAI GACnn'nnGTC

Cuts at:   1222    1222 
   Size:       4526
 
PspOMI G'GGCC_C

Cuts at:    955     955 
   Size:       4526
 
PstI C_TGCA'G

Cuts at:   1573    2258    1573 
   Size:        685    3841
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4526
 
PvuII CAG'CTG

Cuts at:   1080    1951    2311    1080 
   Size:        871     360    3295

  Fragments arranged by size: 

                3295     871     360
 
RsrII CG'GwC_CG

Cuts at:   2721    2721 
   Size:       4526
 
SacI G_AGCT'C

Cuts at:   1192    1540    1192 
   Size:        348    4178
 
SalI G'TCGA_C

Cuts at:   1563    1563 
   Size:       4526
 
SapI GCTCTTCn'nnn_

Cuts at:    473    2549    2759     473 
   Size:       2076     210    2240

  Fragments arranged by size: 

                2240    2076     210
 
SbfI CC_TGCA'GG

Cuts at:   1573    1573 
   Size:       4526
 
ScaI AGT'ACT

Cuts at:   4043    4043 
   Size:       4526
 
SexAI A'CCwGG_T

Cuts at:    926     974     926 
   Size:         48    4478
 
SfcI C'TryA_G

Cuts at:     33    1569    2254    3247    3438      33 
   Size:       1536     685     993     191    1121

  Fragments arranged by size: 

                1536    1121     993     685     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1445    1445 
   Size:       4526
 
SfoI GGC'GCC

Cuts at:    556    1958    2206     556 
   Size:       1402     248    2876

  Fragments arranged by size: 

                2876    1402     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4526
 
SgrAI Cr'CCGG_yG

Cuts at:    513     513 
   Size:       4526
 
SmaI CCC'GGG

Cuts at:    274    1548     274 
   Size:       1274    3252
 
SmlI C'TyrA_G

Cuts at:    961    1681    2596    3088    3350     961 
   Size:        720     915     492     262    2137

  Fragments arranged by size: 

                2137     915     720     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4526
 
SphI G_CATG'C

Cuts at:   1077    1579    2079    1077 
   Size:        502     500    3524

  Fragments arranged by size: 

                3524     502     500
 
StuI AGG'CCT

Cuts at:    792     967     792 
   Size:        175    4351
 
StyI C'CwwG_G

Cuts at:   1004    1660    2014    1004 
   Size:        656     354    3516

  Fragments arranged by size: 

                3516     656     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2109    2242    2701    2910    2109 
   Size:        133     459     209    3725

  Fragments arranged by size: 

                3725     459     209     133
 
TatI w'GTAC_w

Cuts at:    425    4041     425 
   Size:       3616     910
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    767     799    4043    4075     767 
   Size:         32    3244      32    1218

  Fragments arranged by size: 

                3244    1218      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    928    1363    2323     928 
   Size:        435     960    3131

  Fragments arranged by size: 

                3131     960     435
 
XbaI T'CTAG_A

Cuts at:   1557    1557 
   Size:       4526
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    811    2021     811 
   Size:       1210    3316
 
XmaI C'CCGG_G

Cuts at:    272    1546     272 
   Size:       1274    3252
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BmtI     BpmI
   Bpu10I    BsaXI    BsiWI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I
     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmlI     PsiI    PspXI     PsrI    SacII    SanDI
    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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