Restriction map for pF1KB5815
 (Linear) (Six-base) MAPSORT of: KIBB5815.seq  Check: 8813  from: 1  to: 1413



KIBB5815.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AleI CACnn'nnGTG

Cuts at:      0    1300    1413 
   Size:       1300     113
 
AlwNI CAG_nnn'CTG

Cuts at:      0     355    1332    1413 
   Size:        355     977      81

  Fragments arranged by size: 

                 977     355      81
 
ApaI G_GGCC'C

Cuts at:      0     851    1413 
   Size:        851     562
 
ApoI r'AATT_y

Cuts at:      0     585    1413 
   Size:        585     828
 
AvaI C'yCGr_G

Cuts at:      0     164    1413 
   Size:        164    1249
 
BanI G'GyrC_C

Cuts at:      0     408     446    1263    1413 
   Size:        408      38     817     150

  Fragments arranged by size: 

                 817     408     150      38
 
BanII G_rGCy'C

Cuts at:      0     851     893    1084    1413 
   Size:        851      42     191     329

  Fragments arranged by size: 

                 851     329     191      42
 
BbeI G_GCGC'C

Cuts at:      0     450    1413 
   Size:        450     963
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1337    1413 
   Size:       1337      76
 
BglII A'GATC_T

Cuts at:      0    1280    1413 
   Size:       1280     133
 
BlpI GC'TnA_GC

Cuts at:      0    1030    1413 
   Size:       1030     383
 
Bme1580I G_kGCm'C

Cuts at:      0     413     851    1268    1413 
   Size:        413     438     417     145

  Fragments arranged by size: 

                 438     417     413     145
 
BmgBI CAC'GTC

Cuts at:      0     279    1413 
   Size:        279    1134
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     282     547    1413 
   Size:        282     265     866

  Fragments arranged by size: 

                 866     282     265
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     624     741    1314    1413 
   Size:        624     117     573      99

  Fragments arranged by size: 

                 624     573     117      99
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838    1413 
   Size:        838     575
 
BsaAI yAC'GTr

Cuts at:      0     456     921    1413 
   Size:        456     465     492

  Fragments arranged by size: 

                 492     465     456
 
BsaHI Gr'CG_yC

Cuts at:      0     447    1048    1256    1413 
   Size:        447     601     208     157

  Fragments arranged by size: 

                 601     447     208     157
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     226     499    1162    1180    1189    1413 
   Size:        226     273     663      18       9     224

  Fragments arranged by size: 

                 663     273     226     224      18       9
 
BseYI C'CCAG_C

Cuts at:      0     972    1413 
   Size:        972     441
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586    1111    1224    1413 
   Size:        586     525     113     189

  Fragments arranged by size: 

                 586     525     189     113
 
BsiEI CG_ry'CG

Cuts at:      0     135    1413 
   Size:        135    1278
 
BsiHKAI G_wGCw'C

Cuts at:      0     218     302    1036    1084    1413 
   Size:        218      84     734      48     329

  Fragments arranged by size: 

                 734     329     218      84      48
 
BsmI GAATG_Cn'

Cuts at:      0    1026    1413 
   Size:       1026     387
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     302     413     851     893    1036    1084    1268 
   Size:        218      84     111     438      42     143      48     184

Cuts at:   1268    1413 
   Size:        145

  Fragments arranged by size: 

                 438     218     184     145     143     111      84      48
                  42
 
BspHI T'CATG_A

Cuts at:      0    1127    1413 
   Size:       1127     286
 
BsrBI CCG'CTC

Cuts at:      0      24      51      92     608    1413 
   Size:         24      27      41     516     805

  Fragments arranged by size: 

                 805     516      41      27      24
 
BsrDI GCAATG_nn'

Cuts at:      0     947    1413 
   Size:        947     466
 
BsrFI r'CCGG_y

Cuts at:      0      65     134     405    1413 
   Size:         65      69     271    1008

  Fragments arranged by size: 

                1008     271      69      65
 
BssSI C'ACGA_G

Cuts at:      0     244    1413 
   Size:        244    1169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     544    1359    1413 
   Size:        544     815      54

  Fragments arranged by size: 

                 815     544      54
 
BstYI r'GATC_y

Cuts at:      0     674    1235    1280    1413 
   Size:        674     561      45     133

  Fragments arranged by size: 

                 674     561     133      45
 
Bsu36I CC'TnA_GG

Cuts at:      0    1145    1413 
   Size:       1145     268
 
BtgI C'CryG_G

Cuts at:      0     413     884    1413 
   Size:        413     471     529

  Fragments arranged by size: 

                 529     471     413
 
BtsI GCAGTG_nn'

Cuts at:      0     353    1413 
   Size:        353    1060
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     931    1413 
   Size:        931     482
 
EaeI y'GGCC_r

Cuts at:      0    1101    1413 
   Size:       1101     312
 
EarI CTCTTCn'nnn_

Cuts at:      0     365    1413 
   Size:        365    1048
 
EcoICRI GAG'CTC

Cuts at:      0    1082    1413 
   Size:       1082     331
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     871    1413 
   Size:        871     542
 
EcoO109I rG'GnC_Cy

Cuts at:      0     540     848    1116    1329    1413 
   Size:        540     308     268     213      84

  Fragments arranged by size: 

                 540     308     268     213      84
 
HaeII r_GCGC'y

Cuts at:      0     161     206     450    1413 
   Size:        161      45     244     963

  Fragments arranged by size: 

                 963     244     161      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1349    1372    1381    1404    1413 
   Size:       1349      23       9      23       9

  Fragments arranged by size: 

                1349      23      23       9       9
 
KasI G'GCGC_C

Cuts at:      0     446    1413 
   Size:        446     967
 
MslI CAynn'nnrTG

Cuts at:      0     310    1132    1300    1413 
   Size:        310     822     168     113

  Fragments arranged by size: 

                 822     310     168     113
 
MspA1I CmG'CkG

Cuts at:      0     153     972    1413 
   Size:        153     819     441

  Fragments arranged by size: 

                 819     441     153
 
NarI GG'CG_CC

Cuts at:      0     447    1413 
   Size:        447     966
 
NspI r_CATG'y

Cuts at:      0     714     969    1413 
   Size:        714     255     444

  Fragments arranged by size: 

                 714     444     255
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     633    1413 
   Size:        633     780
 
PpuMI rG'GwC_Cy

Cuts at:      0    1116    1413 
   Size:       1116     297
 
PshAI GACnn'nnGTC

Cuts at:      0    1114    1413 
   Size:       1114     299
 
PspOMI G'GGCC_C

Cuts at:      0     847    1413 
   Size:        847     566
 
PvuII CAG'CTG

Cuts at:      0     972    1413 
   Size:        972     441
 
SacI G_AGCT'C

Cuts at:      0    1084    1413 
   Size:       1084     329
 
SapI GCTCTTCn'nnn_

Cuts at:      0     365    1413 
   Size:        365    1048
 
SexAI A'CCwGG_T

Cuts at:      0     818     866    1413 
   Size:        818      48     547

  Fragments arranged by size: 

                 818     547      48
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1337    1413 
   Size:       1337      76
 
SfoI GGC'GCC

Cuts at:      0     448    1413 
   Size:        448     965
 
SgrAI Cr'CCGG_yG

Cuts at:      0     405    1413 
   Size:        405    1008
 
SmaI CCC'GGG

Cuts at:      0     166    1413 
   Size:        166    1247
 
SmlI C'TyrA_G

Cuts at:      0     853    1413 
   Size:        853     560
 
SphI G_CATG'C

Cuts at:      0     969    1413 
   Size:        969     444
 
StuI AGG'CCT

Cuts at:      0     684     859    1413 
   Size:        684     175     554

  Fragments arranged by size: 

                 684     554     175
 
StyI C'CwwG_G

Cuts at:      0     896    1413 
   Size:        896     517
 
TatI w'GTAC_w

Cuts at:      0     317    1413 
   Size:        317    1096
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     659     691    1413 
   Size:        659      32     722

  Fragments arranged by size: 

                 722     659      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     820    1255    1413 
   Size:        820     435     158

  Fragments arranged by size: 

                 820     435     158
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     703    1413 
   Size:        703     710
 
XmaI C'CCGG_G

Cuts at:      0     164    1413 
   Size:        164    1249
 

 Enzymes that do cut:

     AleI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BbeI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI    BpuEI
    BsaAI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BssSI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     DrdI     EaeI     EarI  EcoICRI    EcoNI
 EcoO109I    HaeII    Hin4I     KasI     MslI   MspA1I     NarI     NspI
    PflMI    PpuMI    PshAI   PspOMI    PvuII     SacI     SapI    SexAI
     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI     StuI     StyI
     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BmtI     BpmI   Bpu10I     BsaI    BsaBI    BsaWI
    BsaXI    BsiWI    BsmBI    BspEI    BspMI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EciI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI     SfcI     SgfI    SnaBI     SpeI     SrfI
     SspI     SwaI    TaqII     XbaI     XhoI     XmnI     ZraI
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