Restriction map for
pF1KB7966
(Circular) (Six-base) MAPSORT of: pF1KB7966.seq Check: 4107 from: 1 to: 4316
pF1KB7966 4316 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 727 1354 727
Size: 627 3689
Acc65I G'GTAC_C
Cuts at: 1332 1332
Size: 4316
AclI AA'CG_TT
Cuts at: 4156 4156
Size: 4316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 1218 1276 2141 2573 3320 3588 668
Size: 550 58 865 432 747 268 1396
Fragments arranged by size:
1396 865 747 550 432 268 58
AflIII A'CryG_T
Cuts at: 1668 2772 3749 1668
Size: 1104 977 2235
Fragments arranged by size:
2235 1104 977
AgeI A'CCGG_T
Cuts at: 2704 2704
Size: 4316
AlwNI CAG_nnn'CTG
Cuts at: 526 1621 3188 526
Size: 1095 1567 1654
Fragments arranged by size:
1654 1567 1095
ApaI G_GGCC'C
Cuts at: 419 419
Size: 4316
ApaLI G'TGCA_C
Cuts at: 3086 3086
Size: 4316
ApoI r'AATT_y
Cuts at: 1320 3669 3925 1320
Size: 2349 256 1711
Fragments arranged by size:
2349 1711 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4316
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
AvaI C'yCGr_G
Cuts at: 211 981 1336 211
Size: 770 355 3191
Fragments arranged by size:
3191 770 355
AvrII C'CTAG_G
Cuts at: 948 948
Size: 4316
BamHI G'GATC_C
Cuts at: 1341 1341
Size: 4316
BanI G'GyrC_C
Cuts at: 485 1332 1746 1994 2029 485
Size: 847 414 248 35 2772
Fragments arranged by size:
2772 847 414 248 35
BanII G_rGCy'C
Cuts at: 148 166 419 859 1330 2360 148
Size: 18 253 440 471 1030 2104
Fragments arranged by size:
2104 1030 471 440 253 18
BbeI G_GCGC'C
Cuts at: 489 1750 1998 489
Size: 1261 248 2807
Fragments arranged by size:
2807 1261 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 625 659 625
Size: 34 4282
BciVI GTATCCnnnnn_n'
Cuts at: 2214 2975 4316 2214
Size: 761 1341 2214
Fragments arranged by size:
2214 1341 761
BclI T'GATC_A
Cuts at: 1836 1836
Size: 4316
BglI GCCn_nnn'nGGC
Cuts at: 423 423
Size: 4316
BglII A'GATC_T
Cuts at: 1831 3868 1831
Size: 2037 2279
BlpI GC'TnA_GC
Cuts at: 1428 1428
Size: 4316
Bme1580I G_kGCm'C
Cuts at: 387 419 1941 2034 3090 387
Size: 32 1522 93 1056 1613
Fragments arranged by size:
1613 1522 1056 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 216 1528 1609 1944 4088 216
Size: 1312 81 335 2144 444
Fragments arranged by size:
2144 1312 444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4316
Bpu10I CC'TnA_GC
Cuts at: 591 968 591
Size: 377 3939
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1492 2371 2863 3161 1492
Size: 879 492 298 2647
Fragments arranged by size:
2647 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 901 4020 901
Size: 3119 1197
BsaAI yAC'GTr
Cuts at: 1669 1669
Size: 4316
BsaBI GATnn'nnATC
Cuts at: 6 809 877 1830 3867 6
Size: 803 68 953 2037 455
Fragments arranged by size:
2037 953 803 455 68
BsaHI Gr'CG_yC
Cuts at: 250 486 1747 1995 2697 4193 250
Size: 236 1261 248 702 1496 373
Fragments arranged by size:
1496 1261 702 373 248 236
BsaWI w'CCGG_w
Cuts at: 1215 1505 2026 2704 2978 3125 3536 1215
Size: 290 521 678 274 147 411 1995
Fragments arranged by size:
1995 678 521 411 290 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 344 470 763 919 1190 344
Size: 126 293 156 271 3470
Fragments arranged by size:
3470 293 271 156 126
BseYI C'CCAG_C
Cuts at: 291 399 412 710 931 1741 2499 3076 3572
Size: 108 13 298 221 810 758 577 496
Cuts at: 3572 291
Size: 1035
Fragments arranged by size:
1035 810 758 577 496 298 221 108
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 4316
BsiEI CG_ry'CG
Cuts at: 104 427 1070 1858 1904 3112 104
Size: 323 643 788 46 1208 1308
Fragments arranged by size:
1308 1208 788 643 323 46
BsiHKAI G_wGCw'C
Cuts at: 229 665 859 1330 2108 2298 3090 229
Size: 436 194 471 778 190 792 1455
Fragments arranged by size:
1455 792 778 471 436 194 190
Bsp1286I G_dGCh'C
Cuts at: 148 166 229 387 419 665 859 1330 1941
Size: 18 63 158 32 246 194 471 611
Cuts at: 1941 2034 2108 2298 2360 3090 148
Size: 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 471 246 194 190 158
93 74 63 62 32 18
BspEI T'CCGG_A
Cuts at: 3536 3536
Size: 4316
BspHI T'CATG_A
Cuts at: 3492 3492
Size: 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1366 1580 1882 2263 1366
Size: 214 302 381 3419
Fragments arranged by size:
3419 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2608 2662 4146 4311 50
Size: 2558 54 1484 165 55
Fragments arranged by size:
2558 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2228 2228
Size: 4316
BsrFI r'CCGG_y
Cuts at: 360 422 2314 2495 2704 360
Size: 62 1892 181 209 1972
Fragments arranged by size:
1972 1892 209 181 62
BsrGI T'GTAC_A
Cuts at: 925 925
Size: 4316
BssHII G'CGCG_C
Cuts at: 364 364
Size: 4316
BssSI C'ACGA_G
Cuts at: 2587 2945 2587
Size: 358 3958
BstAPI GCAn_nnn'nTGC
Cuts at: 1693 1693
Size: 4316
BstBI TT'CG_AA
Cuts at: 2677 2677
Size: 4316
BstXI CCAn_nnnn'nTGG
Cuts at: 299 299
Size: 4316
BstYI r'GATC_y
Cuts at: 1 804 1341 1808 1831 2166 2412 3424 3510
Size: 803 537 467 23 335 246 1012 86
Cuts at: 3510 3522 3868 1
Size: 12 346 449
Fragments arranged by size:
1012 803 537 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 193 236 267 298 445 831 107
Size: 86 43 31 31 147 386 3592
Fragments arranged by size:
3592 386 147 86 43 31 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2266 2447 2610 2266
Size: 181 163 3972
Fragments arranged by size:
3972 181 163
BtsI GCAGTG_nn'
Cuts at: 537 1559 537
Size: 1022 3294
DraI TTT'AAA
Cuts at: 1315 1315
Size: 4316
DraIII CAC_nnn'GTG
Cuts at: 1183 1183
Size: 4316
DrdI GACnn_nn'nnGTC
Cuts at: 2022 2880 2022
Size: 858 3458
EaeI y'GGCC_r
Cuts at: 424 1106 1901 2075 2466 2493 3838 424
Size: 682 795 174 391 27 1345 902
Fragments arranged by size:
1345 902 795 682 391 174 27
EagI C'GGCC_G
Cuts at: 424 1901 424
Size: 1477 2839
EarI CTCTTCn'nnn_
Cuts at: 2339 2549 2339
Size: 210 4106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2524 2834 2980 4126 2524
Size: 310 146 1146 2714
Fragments arranged by size:
2714 1146 310 146
EcoICRI GAG'CTC
Cuts at: 857 1328 857
Size: 471 3845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 1218 1276 2141 2573 3320 3588 668
Size: 550 58 865 432 747 268 1396
Fragments arranged by size:
1396 865 747 550 432 268 58
EcoNI CCTnn'n_nnAGG
Cuts at: 622 1225 622
Size: 603 3713
EcoO109I rG'GnC_Cy
Cuts at: 335 416 1455 335
Size: 81 1039 3196
Fragments arranged by size:
3196 1039 81
EcoRI G'AATT_C
Cuts at: 1320 1320
Size: 4316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 4316
FspI TGC'GCA
Cuts at: 2097 2097
Size: 4316
HaeII r_GCGC'y
Cuts at: 298 489 1750 1998 3020 3999 298
Size: 191 1261 248 1022 979 615
Fragments arranged by size:
1261 1022 979 615 248 191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2158 2188 2190 2220 2158
Size: 30 2 30 4254
Fragments arranged by size:
4254 30 30 2
HincII GTy'rAC
Cuts at: 1355 1355
Size: 4316
KasI G'GCGC_C
Cuts at: 485 1746 1994 485
Size: 1261 248 2807
Fragments arranged by size:
2807 1261 248
KpnI G_GTAC'C
Cuts at: 1336 1336
Size: 4316
MluI A'CGCG_T
Cuts at: 3749 3749
Size: 4316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2987 3171 2987
Size: 184 4132
MscI TGG'CCA
Cuts at: 2077 2077
Size: 4316
MslI CAynn'nnrTG
Cuts at: 2305 2432 2305
Size: 127 4189
MspA1I CmG'CkG
Cuts at: 269 447 586 931 1309 1427 1741 2101 3114
Size: 178 139 345 378 118 314 360 1013
Cuts at: 3114 3359 269
Size: 245 1226
Fragments arranged by size:
1226 1013 378 360 345 314 245 178
139 118
NaeI GCC'GGC
Cuts at: 424 2497 424
Size: 2073 2243
NarI GG'CG_CC
Cuts at: 486 1747 1995 486
Size: 1261 248 2807
Fragments arranged by size:
2807 1261 248
NcoI C'CATG_G
Cuts at: 107 193 107
Size: 86 4230
NgoMIV G'CCGG_C
Cuts at: 422 2495 422
Size: 2073 2243
NspI r_CATG'y
Cuts at: 786 1369 1869 2776 786
Size: 583 500 907 2326
Fragments arranged by size:
2326 907 583 500
PasI CC'CwG_GG
Cuts at: 459 459
Size: 4316
PciI A'CATG_T
Cuts at: 2772 2772
Size: 4316
PfoI T'CCnGG_A
Cuts at: 494 494
Size: 4316
PmeI GTTT'AAAC
Cuts at: 1315 1315
Size: 4316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 4284
PpuMI rG'GwC_Cy
Cuts at: 335 335
Size: 4316
PspOMI G'GGCC_C
Cuts at: 415 415
Size: 4316
PstI C_TGCA'G
Cuts at: 1281 1363 2048 1281
Size: 82 685 3549
Fragments arranged by size:
3549 685 82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4316
PvuII CAG'CTG
Cuts at: 931 1309 1741 2101 931
Size: 378 432 360 3146
Fragments arranged by size:
3146 432 378 360
RsrII CG'GwC_CG
Cuts at: 2511 2511
Size: 4316
SacI G_AGCT'C
Cuts at: 859 1330 859
Size: 471 3845
SacII CC_GC'GG
Cuts at: 270 448 270
Size: 178 4138
SalI G'TCGA_C
Cuts at: 1353 1353
Size: 4316
SapI GCTCTTCn'nnn_
Cuts at: 2339 2549 2339
Size: 210 4106
SbfI CC_TGCA'GG
Cuts at: 1363 1363
Size: 4316
ScaI AGT'ACT
Cuts at: 3833 3833
Size: 4316
SfcI C'TryA_G
Cuts at: 33 733 1277 1359 2044 3037 3228 33
Size: 700 544 82 685 993 191 1121
Fragments arranged by size:
1121 993 700 685 544 191 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 423 423
Size: 4316
SfoI GGC'GCC
Cuts at: 487 1748 1996 487
Size: 1261 248 2807
Fragments arranged by size:
2807 1261 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
SgrAI Cr'CCGG_yG
Cuts at: 360 360
Size: 4316
SmaI CCC'GGG
Cuts at: 213 983 1338 213
Size: 770 355 3191
Fragments arranged by size:
3191 770 355
SmlI C'TyrA_G
Cuts at: 1471 2386 2878 3140 1471
Size: 915 492 262 2647
Fragments arranged by size:
2647 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4316
SphI G_CATG'C
Cuts at: 1369 1869 1369
Size: 500 3816
StyI C'CwwG_G
Cuts at: 107 193 407 566 623 948 1450 1804 107
Size: 86 214 159 57 325 502 354 2619
Fragments arranged by size:
2619 502 354 325 214 159 86 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1056 1899 2032 2491 2700 1056
Size: 843 133 459 209 2672
Fragments arranged by size:
2672 843 459 209 133
TatI w'GTAC_w
Cuts at: 925 3831 925
Size: 2906 1410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3833 3865 3833
Size: 32 4284
Tth111I GACn'n_nGTC
Cuts at: 2113 2113
Size: 4316
XbaI T'CTAG_A
Cuts at: 900 1347 900
Size: 447 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 243 1234 1811 243
Size: 991 577 2748
Fragments arranged by size:
2748 991 577
XmaI C'CCGG_G
Cuts at: 211 981 1336 211
Size: 770 355 3191
Fragments arranged by size:
3191 770 355
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI
BsaXI BsiWI BsmI BsmBI BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI